In Vitro Assessment of the Equine Hoof Wall Strains in Flat Weight Bearing and After Heel Elevation     

Maria Clia Ramos Bellenzani PhD  Associate Professor  Júlia Maria D'andrea Greve PhD  Professor-Physiatrics  Csar Augusto Martins Pereira 《Journal of Equine Veterinary Science》2007,27(11):475-480

Horseshoeing is a common practice, but effects on the hoof wall are poorly understood. Strain gauges were used to document and compare hoof behavior in vitro during flat weight bearing and after artificial heel elevation. Ten front limbs of Thoroughbred race horses, shod with conventional flat shoes, were used. Eight strain gauges were symmetrically distributed around the toe, quarters, and heels. Each limb was mounted to a testing machine (Kratos K5002; Kratos Dynamômetros, Ltda., Cotia-SP-Brazil) and subjected to a load equivalent to 30% of the donor's body weight. Strains (μ) were acquired by means of a computerized system and the results compared using Friedman and Wilcoxon statistical tests. There was greater strain variation when the heels were elevated. Compression predominated during flat weight bearing, with a tendency to horizontal traction after heel elevation. The changes in strain caused by heel elevation were not always symmetrical. Elevation of the heels tensed the toe and the medial quarter horizontally, increased load at the posterior portion of the hoof capsule, and hindered its expansion.  相似文献   

Treatment of doxorubicin extravasation with intravenous dexrazoxane in a cat     

Mahoney JA  Bergman PJ  Camps-Palau MA  Hull TL 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(4):872-873

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A monoclonal antibody against a canine CD45 homologue: analysis of tissue distribution, biochemical properties and in vitro immunological activity     

Aguiar PH  Barrouin-Melo SM  Franke CR  dos Santos RR  Silva TM  Mengel JO  dos-Santos WL  Pontes-de-Carvalho L 《Veterinary journal (London, England : 1997)》2007,173(1):158-166

This report describes the characterisation of a monoclonal antibody (mAb), AB6, which recognises specifically a cluster of canine leukocyte surface molecules. The immunogen used for obtaining the AB6 mAb was a lysate of canine peripheral blood mononuclear cells (PBMC). This novel mAb belongs to the IgG2a isotype, and reacted in Western blot with four different canine leukocyte glycoproteins with apparent molecular weights of 180, 190, 205 and 220 kDa. The AB6 mAb recognised the majority of canine peripheral blood leukocytes as determined by flow cytometry (97%). It also exhibited a broad reactivity pattern against lymphoid and myeloid cells, inhibited the proliferation of mitogen-stimulated canine PBMC and did not recognise human PBMC and murine splenocytes. The biochemical properties, cell and tissue specificity, and in vitro biological activity of the AB6 mAb indicate that it recognises a canine CD45 homologue. The mAb could become a valuable diagnostic and research tool for the evaluation of immune functions in dogs.  相似文献   

A ten-year review of commercial vaccine performance for control of tick infestations on cattle   总被引：3，自引：0，他引：3  

de la Fuente J  Almazán C  Canales M  Pérez de la Lastra JM  Kocan KM  Willadsen P 《Animal health research reviews / Conference of Research Workers in Animal Diseases》2007,8(1):23-28

Ticks are important ectoparasites of domestic and wild animals, and tick infestations economically impact cattle production worldwide. Control of cattle tick infestations has been primarily by application of acaricides which has resulted in selection of resistant ticks and environmental pollution. Herein we discuss data from tick vaccine application in Australia, Cuba, Mexico and other Latin American countries. Commercial tick vaccines for cattle based on the Boophilus microplus Bm86 gut antigen have proven to be a feasible tick control method that offers a cost-effective, environmentally friendly alternative to the use of acaricides. Commercial tick vaccines reduced tick infestations on cattle and the intensity of acaricide usage, as well as increasing animal production and reducing transmission of some tick-borne pathogens. Although commercialization of tick vaccines has been difficult owing to previous constraints of antigen discovery, the expense of testing vaccines in cattle, and company restructuring, the success of these vaccines over the past decade has clearly demonstrated their potential as an improved method of tick control for cattle. Development of improved vaccines in the future will be greatly enhanced by new and efficient molecular technologies for antigen discovery and the urgent need for a tick control method to reduce or replace the use of acaricides, especially in regions where extensive tick resistance has occurred.  相似文献   

Cytokine mRNA profiles in pigs exposed prenatally and postnatally to Schistosoma japonicum     

Techau ME  Johansen MV  Aasted B  Lind P  Ornbjerg N  Oswald IP 《Veterinary research》2007,38(1):25-36

The pig is a natural host for Schistosoma japonicum and a useful animal model of human infection. The aim of the present study was to assess the differences between the cytokine profiles in prenatally or postnatally S. japonicum exposed pigs. Seven prenatally exposed pigs, 7 postnatally exposed pigs and 4 uninfected control pigs were compared 27 weeks post exposure. Variables included worm burdens, tissue egg counts, liver pathology and mRNA levels of IL-2, IL-4, IL-10, IL-12, TNF-alpha, TGF-beta1 and IFN-gamma in the liver and the caecum, assessed by RT-PCR. Infection intensity and level of septal fibrosis were significantly higher in the postnatal group compared to the prenatal group (P < 0.05). A significant increase of IL-4 (P < 0.01), IL-10 (P < 0.01), IL-12 (P < 0.01) and TNF-alpha (P < 0.05) mRNA level was also observed in the caecum of prenatally infected animals compared to the control group (P < 0.01). The prenatal group showed higher levels of TGF-beta1 in the liver compared with the postnatally infected group (P< 0.05) and the control group (P< 0.01). This suppressive immune response correlated with previously reported low hepatic pathogenesis in prenatally exposed pigs.  相似文献   

Risk factors associated with sero-positivity to Toxoplasma gondii in captive neotropical felids from Brazil     

Ramos Silva JC  Marvulo MF  Dias RA  Ferreira F  Amaku M  Adania CH  Ferreira Neto JS 《Preventive veterinary medicine》2007,78(3-4):286-295

From September 1995 to February 2001, blood samples were collected from 865 neotropical felids belonging to 8 different species. These animals were housed in 86 institutions located in 78 cities of 20 Brazilian states. Our goal was to identify the risk factors associated with sero-positivity to Toxoplasma gondii in captive neotropical felids from Brazil. All serum samples were tested by the modified agglutination test (MAT), using formalin-fixed whole tachyzoites and mercaptoethanol. For each animal an individual questionnaire was filled with questions about tattoo number, felid species, age, sex, origin, number of animals in the group, introduction of new animals in the group, time in the institution, eating meat previously frozen for a period <7 days in the last 6 months, eating meat of run-over or euthanized animals in the last 6 months, predation of rodents or birds in the last 6 months and presence of domestic cats near the enclosures in the last 6 months. The total sero-prevalence was 55% (95% CI: 52%, 57%). We estimated a prevalence of 46% (95% CI: 40%, 54%) for jaguarundi (Puma yagouaroundi); 58% (95% CI: 53%, 63%) for ocelot (Leopardus pardalis); 50% (95% CI: 45%, 56%) for oncilla (L. tigrinus); 54% (95% CI: 46%, 62%) for margay (L. wiedii); 12% (95% CI: 4%, 31%) for Pampas-cat (L. colocolo); 83% (95% CI: 65%, 93%) for Geoffroy's-cat (L. geoffroyi); 64% (95% CI: 50%, 68%) for jaguar (Panthera onca) and 48% (95% CI: 42%, 54%) for puma (Puma concolor). Multiple logistic regression was used to evaluate the association between the variables in the questionnaire and sero-positivity to T. gondii. We concluded that the independent risk factors for toxoplasmosis were: age >3 years (OR = 4.75 [2.75; 8.2]), eating meat previously frozen for a period <7 days (OR = 2.23 [1.24; 4.01]), and consumption of animals that were run-over or euthanized (OR = 1.64; [1.14; 2.37]).  相似文献   

Dermoid sinus and bone defects of the fifth thoracic vertebrae in a shih-tzu     

Colón JA  Maritato KC  Mauterer JV 《The Journal of small animal practice》2007,48(3):180

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Synthesis,insecticidal activity,and ensembled docking of nitroguanidines bearing S- and R-proline     

Alfredo Rodríguez-Hernández  Israel Bonilla-Landa  Abraham Vidal-Limon  Arturo Ibarra-Juárez  Felipe Barrera-Méndez  Francisco Javier Enríquez Medrano  Ramón E. Díaz de León  José Luis Olivares-Romero 《Pest management science》2023,79(5):1912-1921

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First report of Seville root-knot nematode (Meloidogyne hispanica) infecting tobacco in the Brazil     

de Ramos  Eduarda Kalena Kirsch  Pazdiora  Paulo Cesar  Dallagnol  Leandro José  de Souza  Mayara Rodrigues  de Araujo Filho  Jeronimo Vieira 《植物病害和植物保护杂志》2023,130(1):211-214

Journal of Plant Diseases and Protection - Tobacco (Nicotiana tabacum L.) is one of the major nonfood crops in the Southern of Brazil. During 2019/2020 and 2020/2021 crop seasons, Root Knot...  相似文献   

Being exposed to low concentrations of pirimiphos-methyl and chlorfenapyr has detrimental effects on the mobility of Trogoderma granarium     

Nickolas G. Kavallieratos  Maria C. Boukouvala  Artemis Phoebe A. Pappa  Angelo Canale  Giovanni Benelli 《Pest management science》2023,79(12):5230-5236

BACKGROUND

Sublethal effects of insecticides may negatively affect several biological and behavioral traits of insects. The lethal effects of pirimiphos-methyl and chlorfenapyr have been previously showed on Trogoderma granarium, but little knowledge is available about their sublethal effects at low concentrations on both sexes. Herein, the sublethal effects of pirimiphos-methyl and chlorfenapyr on the mobility of T. granarium males and females were investigated.

RESULTS

Lethal concentration (LC) values of pirimiphos-methyl and chlorfenapyr were lower for T. granarium females than males. LC values on males were LC₁₀ = 0.000788 and 0.00139 mg active ingredient (a.i.) cm⁻², LC₃₀ = 0.00350 and 0.00535 mg a.i. cm⁻², and LC₅₀ = 0.00986 and 0.0136 mg a.i. cm⁻² for pirimiphos-methyl and chlorfenapyr respectively. LC on females were LC₁₀ = 0.000704 and 0.00110 mg a.i. cm⁻², LC₃₀ = 0.00323 and 0.00428 mg a.i. cm⁻², and LC₅₀ = 0.00925 and 0.0110 mg a.i. cm⁻² for pirimiphos-methyl and chlorfenapyr respectively. The walking duration of beetles exposed to LC₃₀ of pirimiphos-methyl was significantly lower than the individuals exposed to LC₁₀ and LC₃₀ of both insecticides and control ones. Pirimiphos-methyl LC₃₀-exposed males remained more time on their back (101.7 s) than females (46.9 s), while the latter stayed immobile longer than males (381.7 s versus 371.9 s). The highest speed was recorded for control beetles (14.17 mm s⁻¹ females vs. 12.44 mm s⁻¹ males), while the lowest speed was observed in pirimiphos-methyl LC₃₀-treated males (8.36 mm s⁻¹) and females (9.66 mm s⁻¹).

CONCLUSIONS

Overall, males and females exposed to low concentrations of pirimiphos-methyl and chlorfenapyr showed reduced motility. This knowledge can be exploited further to unlock behavioral effects of insecticides for effective pest management programs in warehouses. © 2023 Society of Chemical Industry.  相似文献