USE OF ULTRASOUND TO DIAGNOSE DIAPHRAGMATIC RUPTURE IN DOGS AND CATS     

Giliola  Spattini  DVM  Federica  Rossi  DVM  Massimo  Vignoli  DVM  Christopher R.  Lamb  MA  VetMB 《Veterinary radiology & ultrasound》2003,44(2):226-230

Twenty-one cats and six dogs that presented to a first-opinion clinic with signs of dyspnea and muffled cardiac auscultation received ultrasonography to look for signs of diaphragmatic rupture. The presence or absence of diaphragmatic rupture was subsequently determined on the basis of unequivocal radiographic signs, surgical findings, or necropsy. Consistent findings in animals with diaphragmatic rupture were irregular or asymmetric cranial aspect of the liver and abdominal viscera in the thorax. Accuracy of ultrasonography was 25/27 (93%). One false-negative result occurred in a cat with a chronic diaphragmatic rupture in which adhesions between the liver and lung simulated the appearance of an intact diaphragm. One false-positive result occurred in a dog with an abscess involving the left lung and pleural cavity, which was misinterpreted as the stomach. The results of this study support use of ultrasonography in animals with suspected diaphragmatic rupture.  相似文献   

Toxicity and persistence of three pyrethroids for the control of cereal bugs on common wheat     

Pansa  Marco G.  Blandino  Massimo  Ingegno  Barbara L.  Ferrari  Ester  Reyneri  Amedeo  Tavella  Luciana 《Journal of pest science》2015,88(1):201-208

Journal of Pest Science - Sunn pests are responsible for serious kernel damage on wheat (Triticum aestivum L., Poaceae), affecting bread-making quality from their feeding at the late milk-ripe...  相似文献   

Molecular genotyping of “Rizor” and “Holly” rhizomania resistances in sugar beet     

Piergiorgio Stevanato  Marco De Biaggi  Chiara Broccanello  Enrico Biancardi  Massimo Saccomani 《Euphytica》2015,206(2):427-431

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Pasta made from durum wheat semolina fermented with selected lactobacilli as a tool for a potential decrease of the gluten intolerance   总被引：3，自引：0，他引：3  

di Cagno R  de Angelis M  Alfonsi G  de Vincenzi M  Silano M  Vincentini O  Gobbetti M 《Journal of agricultural and food chemistry》2005,53(11):4393-4402

A pool of selected lactic acid bacteria was used to ferment durum wheat semolina under liquid conditions. After fermentation, the dough was freeze-dried, mixed with buckwheat flour at a ratio of 3:7, and used to produce the "fusilli" type Italian pasta. Pasta without prefermentation was used as the control. Ingredients and pastas were characterized for compositional analysis. As shown by two-dimensional electrophoresis, 92 of the 130 durum wheat gliadin spots were hydrolyzed almost totally during fermentation by lactic acid bacteria. Mass spectrometry matrix-assisted laser desorption/ionization time-of-flight and reversed phase high-performance liquid chromatography analyses confirmed the hydrolysis of gliadins. As shown by immunological analysis by R5-Western blot, the concentration of gluten decreased from 6280 ppm in the control pasta to 1045 ppm in the pasta fermented with lactic acid bacteria. Gliadins were extracted from fermented and nonfermented durum wheat dough semolina and used to produce a peptic-tryptic (PT) digest for in vitro agglutination tests on cells of human origin. The whole PT digests did not cause agglutination. Affinity chromatography on Sepharose-6-B mannan column separated the PT digests in three fractions. Fraction C showed agglutination activity. The minimal agglutinating activity of fraction C from the PT digest of fermented durum wheat semolina was ca. 80 times higher than that of durum wheat semolina. Pasta was subjected to sensory analysis: The scores for stickiness and firmness were slightly lower than those found for the pasta control. Odor and flavor did not differ between the two types of pasta. These results showed that a pasta biotechnology that uses a prefermentation of durum wheat semolina by selected lactic acid bacteria and tolerated buckwheat flour could be considered as a novel tool to potentially decrease gluten intolerance and the risk of gluten contamination in gluten-free products.  相似文献   

The proportion of immature breeders as a reliable early warning signal of population decline: evidence from the Spanish imperial eagle in Doñana     

Miguel Ferrer  Vincenzo Penteriani  Massimo Pandolfi 《Biological conservation》2003,114(3):463-466

Methods to evaluate population trends have recently received particular attention because of perceived declines in several species during the 20th century. We investigated whether age at first breeding could be used as an “early warning signal” to detect possible changes in population trends in long-lived species with deferred maturity using data from the Spanish imperial eagle (Aquila adalberti) population in Doñana National Park (Spain). This bird of prey is an endangered species that has suffered a rapid decline in this population during the last 10 years. As a result of our 27-year monitoring (1976-2002) study, we detected that an increase in immature breeding birds occurred before population decline became evident. The proportion of immature-plumaged breeders in the population was significantly higher during the period of decline than during the period of stability. In our case, more than 10% of immature breeders can be considered as an “early warning signal” that anticipates population decline. Owing to the ignorance of this warning signal, urgent actions for the recovery of this eagle population started 10 years later than necessary, and when population size had been reduced.  相似文献   

Total and EDTA-extractable element contents in ophiolitic soils from Tuscany (Italy)     

Massimo Angelone  Orlando Vaselli  Claudio Bini  Nara Coradossi  Maria G. Pancani 《植物养料与土壤学杂志》1991,154(3):217-223

Total and EDTA extractable element distributions in ophiolitic soils from Tuscany (Central Italy) were studied. The concentration levels recorded for total elements point to a slight pedogeochemical evolution of the soils investigated, since most of them fall in the concentration ranges of the parent materials. The levels of the EDTA extractable elements are consistent with the total element distribution within the soil profiles, and with the weathering patterns of the most common rock-forming minerals. They fall in the ranges which are considered “not toxic” to plants, especially those of heavy metals. It is suggested that the well-known infertility of such soils could be the consequence of major and trace elements synergism.  相似文献   

Incidence and type of brain herniation associated with intracranial meningioma in dogs and cats     

Simone MINATO  Giunio B. CHERUBINI  Daniele DELLA SANTA  Stefano SALVADORI  Massimo BARONI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(2):267

The incidence of brain herniation (BH) in association with intracranial meningioma (ICM) in dogs and cats is poorly described. The aim of this study was to evaluate the rate and type of brain herniations in client-owned dogs and cats with ICMs and to determine the meningioma volume (MV) relative to cranial cavity volume (CCV). A retrospective magnetic resonance imaging (MRI) analysis study of 24 cats and 45 dogs with ICMs was conducted to ascertain the presence and characteristics of BH. MV and CCV were measured and their ratio was calculated for each animal. Correlations of MV/CCV with independent variables were analyzed. BH was encountered in 24/24 cats (100%) and 30/45 dogs (66.7%) with ICMs. In cats, the most frequent presentation was foramenal herniation (FMH; 23/24, 95.8%), followed by caudotentorial (CTH; 21/24, 87.5%) and subfalcine (SH; 18/24, 75.0%) herniation. In dogs, the most frequent presentation was SH (28/45; 62.2%), followed by CTH (9/45; 20%) and FMH (2/45; 4.4%). Relative to dogs, cats with ICM had greater incidences of FMH (P<0.001) and CTH (P<0.001). Mean MV/CCV ratio was higher in cats (0.098) than in dogs (0.038; P<0.001). The most common clinical sign of ICM was altered behavior in cats (43%, P<0.01) and seizures in dogs (74.4%, P<0.001). In conclusion, cats were found to be more likely than dogs to present FMH and CTH, with a proportionally greater neoplasia volume.  相似文献   

Preventing invasions of Asian longhorn beetle and citrus longhorn beetle: are we on the right track?     

Branco  Sofia  Faccoli  Massimo  Brockerhoff  Eckehard G.  Roux  Géraldine  Jactel  Hervé  Desneux  Nicolas  Gachet  Emmanuel  Mouttet  Raphaelle  Streito  Jean-Claude  Branco  Manuela 《Journal of pest science》2022,95(1):41-66

Two Asian longhorn beetles, Anoplophora glabripennis and Anoplophora chinensis, are among the most serious alien invasive species attacking forest and urban trees, both in North America and Europe. Major efforts have been put into preventing further entry and establishment of the two species as well as promoting their successful eradication. Here, we review these efforts, their progress and outcome, and scientific advancements in monitoring and control methods. The combined international activities and harmonizing legislative changes in detection and eradication methods have proven worthwhile, with more than 45% of eradication programmes successful in the last 12 years. Some countries were able to completely eradicate all populations and others managed to reduce the area affected. Although the costs of the eradication programmes can be very high, the benefits outweigh inaction. Attempts to eradicate A. chinensis have been more challenging in comparison with those targeting A. glabripennis. For both species, efforts are hampered by the ongoing arrival of new beetles, both from their native regions in Asia and from other invaded regions via bridgehead effects. The methods used for eradication have not changed much during the last decade, and host removal is still the method most commonly used. On the other hand, detection methods have diversified during the last decade with advances in semiochemical research and use of detection dogs. The next decade will determine if eradications continue to be successful, particularly in the case of A. chinensis, which has been targeted in some countries for containment instead of eradication.

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Cyclin D1 immunohistochemical expression and somatic mutations in canine oral melanoma     

Clarissa Zamboni  Ginevra Brocca  Serena Ferraresso  Silvia Ferro  Alessandro Sammarco  Chiara Dal Corso  Selina Iussich  Paloma J. de Andres  Elena M. Martìnez de Merlo  Laura Cavicchioli  Valentina Zappulli  Massimo Castagnaro 《Veterinary and comparative oncology》2020,18(2):231-238

Canine oral melanoma (COM) is the most frequent tumour with oral localization in dogs. Copy number gains and amplifications of CCND1, a gene coding for Cyclin D1, are the most frequent chromosomal aberrations described in human non‐UV induced melanomas. Twenty‐eight cases of COM were retrieved from paraffin‐blocks archives. A total of 4 μm thick sections were immunostained with an antibody against human Cyclin D1 and Ki‐67. Cyclin D1 and Ki‐67 expressions were scored through two counting methods. DNA was extracted from 20 μm thick sections of formalin‐fixed paraffin‐embedded blocks. Pathological and surrounding healthy tissue was extracted independently. Cyclin D1 immunolabelling was detected in 69% (18/26) while Ki‐67 was present in 88.5% (23/26) of cases. Statistical analysis revealed correlation between two counting methods for Cyclin D1 (r = 0.54; P = .004) and Ki‐67 (r = 0.56; P = .003). The correlation found between Ki‐67 and Cyclin D1 indexes in 16/26 cases labelled by both antibodies (r = 0.7947; P = .0002) suggests a possible use of Cyclin D1 index as prognostic marker. Polymerase chain reaction analysis on CCND1 coding sequence revealed the presence of nine somatic mutations in seven samples producing synonymous, missense and stop codons. Since none of the single‐nucleotide polymorphisms was found to be recurrent, it is suggested that overexpression of Cyclin D1 may be the consequence of alterations of CCND1 upstream regions or other genetic aberrations not detectable with the methodology used in this study. Future studies are needed to verify the potential use of Cyclin D1 index as prognostic indicator and to highlight the molecular events responsible for Cyclin D1 overexpression in COMs.  相似文献   

Root and stem rot,and wilting of olive tree caused by Dematophora necatrix and associated with Emmia lacerata in Central Italy     

Fusco  Valeria  Pasciuta  Vittorio  Lumia  Valentina  Matere  Antonio  Battaglia  Valerio  Bertinelli  Giorgia  Sansone  Domenico  Brunetti  Angela  Pilotti  Massimo 《European journal of plant pathology / European Foundation for Plant Pathology》2022,163(1):71-96

European Journal of Plant Pathology - Lethal wilting was observed on young olive trees cv Favolosa in a grove in central Italy. White mycelial strands wrapped the basal portion of the stems that...  相似文献