Inclusion of β-1,3/1,6-glucan in the ornamental fish,Jewel tetra (Hyphessobrycon eques), and its effects on growth,blood glucose,and intestinal histology     

Furlan-Murari  Pâmela Juliana  de Lima  Ed Christian Suzuki  de Souza  Felipe Pinheiro  Urrea-Rojas  Angela Maria  Pupim  Andreia Carla Eugenio  de Almeida Araújo  Eduardo José  Meletti  Paulo César  Leal  Cindy Namie Seino  Fernandes  Letícia Lima  Lopera-Barrero  Nelson Mauricio 《Aquaculture International》2022,30(1):501-515

The effects of β-1,3/1,6-glucan on Hyphessobrycon eques were assessed after 42 days of feeding diets containing 0 (control group given commercial feed), 0.5, 1, or 2 g β-glucan/kg diet. In total, 180 fish, with an initial weight of 0.43?±?0.03 g, were used. There were 15 fish in each of twelve 42-L aquariums, and there were 3 aquariums of fish for each dietary treatment. The fish were fed until apparent satiety. Performance parameters (final weight, total length, standard length, feed intake, survival rate, weight gain, feed conversion, specific growth rate, and condition factor) and plasma glucose concentration were measured. Histological analysis of the proximal portion of the intestine (width and height of the villi, depth of the crypts, height of the enterocytes, thickness of the muscle layer, and number of goblet cells) was performed. Different levels of the additive did not influence fish performance (for example, final weight: control: 0.63 g, 0.5: 0.60, 1: 0.58, and 2: 0.61). Likewise, there was no influence on the plasma glucose concentration (control: 81.80 mg/dL, 0.5: 75.33, 1: 85.00, and 2: 81.00) and intestinal morphometry of the animals. However, the results showed that 2.0 g/kg of β-1,3/1,6-glucan provided a greater abundance of goblet cells secreting acidic and neutral mucus present in the epithelium (periodic acid-Schiff: 66.67 cells, Alcian blue pH 1.0: 72,67 cells, and Alcian blue pH 2.5: 95.00 cells), showing significant differences when compared to animals in the control group, which may represent better protection of the intestinal epithelium of H. eques.

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Mapping resistance gene analogs (RGAs) in cultivated tetraploid cotton using RGA-AFLP analysis     

Chen Niu  Yingzhi Lu  Youlu Yuan  Richard G. Percy  Mauricio Ulloa  Jinfa Zhang 《Euphytica》2011,181(1):65-76

Diseases cause significant losses in cotton production throughout the US Cotton Belt. Growing resistant cultivars can significantly improve cotton yields and effectively reduce production inputs. Disease resistance (R) genes have been isolated in numerous plant species and the R genes with domains of nucleotide binding sites (NB) and leucine rich repeats (LRR) represent the largest R gene family. Degenerate primers designed based on conserved motifs of plant disease resistance genes were used alone or in combination with AFLP primers to analyze disease resistance gene analogs (RGAs) in a recombinant inbred line (RIL) population of Pima (Gossypium barbadense) 3–79 and Upland cotton (G. hirsutum) line NM 24016. Eighty-eight polymorphic RGA markers were amplified by 8 pairs of RGA degenerate primers, while 131 polymorphic RGA-AFLP markers were produced from six pairs of RGA-AFLP primer combinations. Of the 219 polymorphic RGA and RGA-AFLP markers that were identified, 212 were assigned to 18 chromosomes and linkage groups based on existing SSR markers that are on known chromosomes. However, the RGA and RGA-AFLP markers are not evenly distributed among chromosomes in that 189 RGA and RGA-AFLP markers (88%) are assigned onto three “giant” chromosomes, i.e., C6, C12, and C15, suggesting RGA clusters in the cotton genome. Several RGA and RGA-AFLP markers were mapped to the same linkage group carrying a root-knot nematode resistance gene. The identification and mapping of RGA and RGA-AFLP markers provide a framework to facilitate marker-assisted selection of disease resistance in cotton breeding and to understand the physical relationship of cotton resistance genes.  相似文献   

Identification and isolation of psittacid herpesvirus from psittacids in Brazil     

Luppi MM  Luiz AP  Coelho FM  Malta Mde C  Preis IS  Ecco R  da Fonseca FG  Resende M 《Veterinary microbiology》2011,154(1-2):69-77

Psittacid herpesvirus (PsHV) was isolated from 41 birds kept in captivity in Belo Horizonte, Minas Gerais/Brazil using chicken embryo fibroblasts (CEF) cell cultures. For this study, leukocytes or cloacal swabs of live birds were used. Also, portions of liver, spleen or kidney from birds collected at necropsy were utilized for these tests. PCR tests confirmed the presence of PsHV in 100% of samples. Thirty-three of the PCR products were sequenced and the results disclosed a 99% and 100% identity when compared with other sequences PsHV-1 (AY372243.1 and AF261756.1), previously deposited in GenBank. In addition, histopathology was performed and 19 of the 29 birds contained random multifocal lymphoplasmacytic hepatitis with necrotic foci, suggestive of viral infection. Three samples were examined by electron microscopy to visualize the viral particles obtained from cell culture. The viral structures measured 269 nm in average, had envelopes with an icosahedral capsid and tegument, consistent with herpesvirus. Thus, a total of 41 isolates were obtained from PsHV cell cultivation in CEF, confirming the circulation of the virus between parrots kept in captivity in Belo Horizonte, and affirming the importance of further studies in this area.  相似文献   

Identification and characterization of genes differentially expressed in cherimoya (Annona cherimola Mill) after exposure to chilling injury conditions     

González-Agüero M  Cifuentes-Esquivel N  Ibañez-Carrasco F  Gudenschwager O  Campos-Vargas R  Defilippi BG 《Journal of agricultural and food chemistry》2011,59(24):13295-13299

Cherimoyas (Annona cherimola), like other subtropical/tropical fruits, are susceptible to damage from exposure to temperatures between 0 and 5 °C (chilling injury, CI), which may affect fruit quality. To increase our understanding of the molecular mechanisms involved in the CI response, a forward suppression subtractive hybridization (SSH) cDNA library was constructed. In this work, we obtained 75 genes that could potentially be involved in the CI response. The CI induced activation of genes that are involved in a range of metabolic pathways, such as primary metabolism, transport, and endomembrane traffic, among others. We also characterized the expression of 12 selected genes in different A. cherimola tissues by polymerase chain reaction (PCR), and we confirmed the differential expression of a subset in CI fruits by real-time quantitative PCR (qPCR). The expression of six A. cherimola genes: annexin (AcAnex), UDP-glucose pyrophosphorylase (AcUGP), syntaxin of plants 71 (AcSyp71), 1-aminocyclopropane-1-carboxylic-acid synthase (AcACS), ubiquitin carrier-like protein (AcUCP), and enolase (AcEnol), was up-regulated after cold storage for 12 days at 0 °C. These results imply that selected genes could be related to the development of internal browning observed in cherimoyas after exposure to CI conditions. The information generated in this study provides new clues that may aid in understanding the cherimoya ripening process.  相似文献   

Effects of habitat fragmentation on pollen flow and genetic diversity of the endangered tropical tree Swietenia humilis (Meliaceae)     

Fernando Rosas  Mauricio Quesada  Jorge A. Lobo  Victoria L. Sork 《Biological conservation》2011,144(12):3082-3088

Fragmentation of tropical forest represents a major threat to some tree populations by reducing local population size and gene flow from other populations. Both processes can decrease outcrossing rates and genetic variation in remnant stands. Despite these risks, some tree species have pollen vectors that mitigate these negative consequences for fragmented populations. In this paper, we assess both pollen flow and diversity of pollen sources in continuous forest and isolated stands of Swietenia humilis, a tropical tree species pollinated by small insects. Using seven nuclear microsatellite markers, we test the hypothesis that genetic diversity and the number of pollen donors are lower in remnant populations. Results show that allelic richness of seeds is lower in isolated populations (6.1 vs. 8.3 alleles per locus), even though adult populations do not show this difference.Pollen pool structure is greater in isolated patches (Φ_Iso = 0.26) than in continuous forest (Φ_For = 0.14), which yields estimates of the average effective number of pollen donors (N_ep) of 1.9 and 3.6 respectively. In addition, estimates of number of sires per mother indicate that isolated trees have half the number of pollen sources (4.98) than trees in the forest (9.8). Although extensive pollen movement (>2000 m) was recorded on both habitat conditions, indicating that fragmented patches are not isolated from pollen-mediated gene flow, this extensive pollen flow among trees in fragmented landscapes may not serve to counteract deleterious reproductive and genetic consequences of habitat fragmentation.  相似文献   

DIAGNOSTIC SENSITIVITY OF BONE SCINTIGRAPHY FOR EQUINE STIFLE DISORDERS          下载免费PDF全文

Sarah Graham  Mauricio Solano  James Sutherland‐Smith  Amy F Sato  Louise Maranda 《Veterinary radiology & ultrasound》2015,56(1):96-102

Disorders of the stifle are a common cause of lameness in horses yet the accuracy of scintigraphy for diagnosis of stifle conditions is controversial. The aim of retrospective cross‐sectional study was to determine the diagnostic sensitivity (Se) of bone scintigraphy in detecting stifle disease and to determine if two orthogonal scintigraphic images improve diagnostic Se. Horses that underwent scintigraphic examination during a two‐year period were included. Horses were divided into two groups: group 1 (N = 23) had lameness that was localized to the stifle by intra‐articular analgesia and group 2 (N = 182) had lameness that was localized to a different location. Scintigraphic studies (one image or two images) were independently and retrospectively analyzed by two radiologists (R1 and R2). Sensitivity, specificity (Sp) and predictive values (PV), and were calculated for each type of study (one image or two images) and for each radiologist (R1 or R2). The Se to detect stifle disorders varied between radiologists (29.2% and 20.8%). The Sp was 84.5% and 88.3%. When two images were evaluated a decrease in the positive PV for both readers occurred. The Cohen kappa coefficient (κ) between readers was poor when one image (0.084) or two images (0.117) were evaluated. Findings from this study indicated that bone‐phase nuclear scintigraphy is reasonably specific but highly insensitive for detecting lameness originating from the stifle in a diverse population of both normal and affected horses. The addition of a caudal scintigraphic image acquisition did not improve diagnostic sensitivity.  相似文献   

Influence of blending on the content of different compounds in the biological aging of sherry dry wines     

Berlanga TM  Peinado R  Millán C  Mauricio JC  Ortega JM 《Journal of agricultural and food chemistry》2004,52(9):2577-2581

Principal components analysis to examine the effect of blending (viz. the mixing and transfer of wine between cask rows in a "criaderas and solera" system) on metabolic activity in flor yeasts during biological aging of sherry dry wines was carried out. The variables used in the analysis were the wine compounds most deeply involved in the flor yeast metabolism, namely ethanol, acetaldehyde, glycerol, acetic acid, and l-proline. The greatest blending effect was found to be on the third and second "criadera", which are the stages where the yeasts show a high metabolic activity. The stages holding the oldest wine (viz. the first criadera and the solera) exhibited no differences before and after blending; therefore, the yeasts have a decreased biological activity in them and physical-chemical aging processes seemingly prevail over it.  相似文献   

Effect of Schizosaccharomyces pombe on aromatic compounds in dry sherry wines containing high levels of gluconic acid     

Peinado RA  Mauricio JC  Medina M  Moreno JJ 《Journal of agricultural and food chemistry》2004,52(14):4529-4534

Volatile compounds have been determined in control dry sherry wines and those supplemented with gluconic acid, which were inoculated with the Schizosaccharomyces pombe 1379 (ATCC 26760) yeast strain. These compounds were grouped, according to volatiles exhibiting the identical odor quality, into nine groups of the same odor character (aromatic series) as a way of establishing the aroma profile for the studied wines. Control and supplemented wines showed changes in the balsamic, spicy, roasty, and fruity aromatic series, and tasters judged the aroma as typical of wines subjected to biological aging. This fission yeast may be used as a treatment to reduce gluconic acid contents in wines obtained from rotten grapes, making feasible the incorporation of these wines into the biological aging process. In addition, this procedure may also help to accelerate the traditional biological aging in sherry winemaking due to the contribution of some specific compounds by S. pombe to the wine.  相似文献   

Diversity of <Emphasis Type="Italic">Ullucus tuberosus</Emphasis> (Basellaceae) in the Colombian Andes and notes on ulluco domestication based on morphological and molecular data     

Mauricio Parra-Quijano  Sauris Panda  Nohra Rodríguez  Elena Torres 《Genetic Resources and Crop Evolution》2012,59(1):49-66

Ullucus tuberosus (common name ‘ulluco’) is a popular tuber crop of Andean highlands. Until now attention has been focused on ulluco from Bolivia, Peru and Ecuador, but little is known about its diversity in Colombia. Thirty-six accessions of cultivated ulluco preserved in the Gene Bank of the Universidad Nacional de Colombia were studied to assess the genetic diversity and spatial genetic structure of Colombian ulluco. We used morphological characters, molecular markers (total proteins, isozymes and RAPDs) and ploidy level. High morphological variability, especially for tuber shape and colour characters, was found. Eight accessions from north-east Colombian Andes showed some typical character-states of wild ulluco, suggesting that they could be partially domesticated forms. Genetic analysis using RAPD markers indicated that these accessions were very similar to other cultivated ullucos belonging to the same region. The regional structure observed in isozyme and RAPD dendrograms was confirmed by AMOVA results (52.6% among-region variation) and the spatial correlogram, showing the presence of two gene pools of ulluco in Colombia. Our results and the accumulated data suggest that ulluco was introduced to Colombia at least two times. Initially, semi-domesticated forms would have come from the central Andes to north-east Colombian Andes, where the native inhabitants would have completed the domestication process. Again, fully domesticated ullucos might have been introduced to south-west Colombian Andes.  相似文献   

Evaluation of vegetation indices and apparent soil electrical conductivity for site-specific vineyard management in Chile     

Rodrigo Ortega-Blu  Mauricio Molina-Roco 《Precision Agriculture》2016,17(4):434-450

Spatial variability of Chilean vineyards, in terms of yield and quality, is high, which fully justifies site-specific management, particularly differential harvest. In this study, the most common zoning tools (NDVI and ECa measurements) were evaluated and compared. Comparisons also included a calibrated GVI. Two contrasting large field experiments (pruning, irrigation, and N fertilization treatments) were established in vineyards to (1) evaluate two vegetation indices: (i) a non-calibrated airplane-based NDVI and (ii) calibrated satellite-based GVI and to (2) evaluate the ECa measurements. The GVI was also assessed at the commercial level, in different vineyards and valleys. The GVI was more sensitive in discriminating grape yields and quality while the NDVI failed to adequately sense vigor patterns and fruit quality in the more homogeneous site. Thus, a calibrated GVI can be recommended as a better tool than NDVI for defining management zones as well as making spatial and temporal comparisons among fields and seasons. In general, ECa explained few differences in the alluvial soil properties and did not predict differences in plant vigor as measured by either vegetation indices, therefore ECa by itself was not a good estimator of the most commonly measured soil properties for establishing management zones in these fields with low variability in terms of EC and other soil characteristics.  相似文献