Assessing genetic diversity between breeds for conservation   总被引：1，自引：0，他引：1  

Miguel Angel Toro 《Zeitschrift für Tierzüchtung und Züchtungsbiologie》2006,123(5):289-289

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Improvements in somatic embryogenesis protocol in Feijoa (Acca sellowiana (Berg) Burret): Induction,conversion and synthetic seeds     

Gabriela Claudia Cangahuala-Inocente  Lírio Luiz Dal Vesco  Douglas Steinmacher  Antonio Carlos Torres  Miguel Pedro Guerra 《Scientia Horticulturae》2007

Pineapple guava (Acca sellowiana) syn. Feijoa sellowiana, a Brazilian indigenous Myrtaceae is under domestication in South Brazil. Previous works showed that this species is responsive to somatic embryogenesis and recalcitrant to conventional methods of clonal propagation. In the present work it was evaluated the role of components of culture medium in the induction and development of somatic embryos. The technology of synthetic seeds was also evaluated. Zygotic embryos were inoculated in LPm medium supplemented with 8 mM glutamic acid and 8 mM l-glutamine, 2,4-dichlophenoxiacetic acid (20 μM) and myo-inositol. For conversion of somatic embryos and synthetic seeds it was tested the effect of 6-benzylaminopurine and gibberellic acid combined or not with activated charcoal. The highest values for embryogenetic induction (100%) and number of somatic embryos/explant (113) were observed in the LPm medium supplemented with Glu (8 mM), and 2,4-D. The culture medium supplemented with BA (0.5 μM) and GA₃ (1 μM) and activated charcoal (1.5 g L⁻¹) enhanced the conversion of somatic embryos to plantlets. Pre-germinated somatic embryos encapsulated in sodium alginate with BA (0.5 μM) and GA₃ (1 μM) developed radicles. The use of synthetic seed was a requisite for the survival of plantlets.  相似文献   

Lack of biocontrol capacity in a non-pathogenic mutant of <Emphasis Type="Italic">Fusarium oxysporum</Emphasis> f. sp. <Emphasis Type="Italic">melonis</Emphasis>     

Floriane L’Haridon  Sébastien Aimé  Claude Alabouvette  Chantal Olivain 《European journal of plant pathology / European Foundation for Plant Pathology》2007,118(3):239-246

The aim of this study was to assess the biocontrol capacity of rev157, a non-pathogenic mutant of a pathogenic strain of Fusarium oxysporum f. sp. melonis (Fom24). Inoculated in association with the virulent parental strain, the mutant rev157 did not protect the host plant (muskmelon) against infection by Fom24. Applied on flax, a non-host plant, the mutant rev157 was not able to protect it against its specific pathogen F. oxysporum f. sp. lini. On the contrary the parental strain Fom24 did protect flax as well as a soil-borne biocontrol strain (Fo47). Since the mutant rev157 was affected neither in its growth in vitro nor in its capacity to penetrate into the roots, it can be speculated that the mutation has affected traits responsible for interactions within the plant. In F. oxysporum the pair of strains Fom24/rev157 is a good candidate to identify genes involved in the biocontrol capacity of F. oxysporum and to test the hypothesis of a link between capacity to induce the disease and capacity to induce resistance in the plant.  相似文献   

Advances and Perspectives in the Biological Control of Greenhouse Pests with Special Reference to Colombia     

Clara I. Nicholls  Michael P. Parrella  Miguel A. Altieri 《Integrated Pest Management Reviews》1998,3(2):99-109

This paper analyses the state of the art of biological control of greenhouse pests with special reference to Colombia, one of the world's largest producers of cut flowers. After a discussion of the use and impacts of selected natural enemies on a variety of arthropod pests in greenhouses, suggestions on how to enhance the effectiveness of inundative release strategies further to achieve successful biological control of key pests are provided.  相似文献   

RECENT PROGRESS IN MALACOLOGY     

Dall WH 《Science (New York, N.Y.)》1898,7(167):334-337

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Real time polymerase chain reaction for rapid and quantitative determination of Cystofilobasidium infirmominiatum on the surfaces of apple,pear, and sweet cherry fruit     

Robert A. Spotts  Kelly M. Wallis  Maryna Serdani  Daniel T. O’Gorman  Peter L. Sholberg 《Postharvest Biology and Technology》2009,51(2):227-231

The objectives of this study were to develop primers and a real time PCR protocol for the postharvest biocontrol yeast Cystofilobasidium infirmominiatum (Cim). The application of this technology was developed to quantify Cim on the surfaces of apple, two pear cultivars, and sweet cherry fruit treated over a range of concentrations. Statistically significant relationships were observed between Cim DNA on fruit surfaces, expressed as μg/m², and CFU/L of dip suspensions for apple, pear, and sweet cherry. In addition, the relationship for each fruit was significantly different from the other three fruits. Threshold values of concentrations of Cim DNA on the fruit surface were calculated based on regression equations and a dose of 2.0 × 10¹¹ CFU/L of dip suspension, the dose for optimum decay control, and were 4.8, 7.0, 16.5, and 25.2 μg/m² for Bosc pear, Lapins sweet cherry, d’Anjou pear, and Golden Delicious apple, respectively. Monitoring Cim DNA concentration on fruit surfaces will assure that Cim is being properly applied to fruit and that a sufficient number of cells are present for optimum decay control.  相似文献   

RECENT WORK ON BRACHIOPODS     

Dall WH 《Science (New York, N.Y.)》1884,3(58):325

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Public awareness activities on Eichhornia crassipes in the Victoria Falls World Heritage Site,Livingstone, Zambia1     

M. Nang’alelwa 《EPPO Bulletin》2008,38(3):464-469

Invasive Alien Species (IAS) are a major threat to biodiversity and national economies worldwide. Public awareness and support is critical for the success of any interventions aimed at controlling and managing such invasions. Various communication tools have been identified for the implementation of public awareness programs. Assessment of public perceptions, attitudes and knowledge levels should be an integral part of any public awareness activities. This paper reports the communication actions undertaken in the Victoria Falls World Heritage Site by the Removing Barriers to Invasive Plant Management in Africa (RBIPMA) project during the year 2007. Several awareness meetings with various stakeholders were conducted during the year. A number of posters, leaflets, brochures and newsletters have been produced. Production of radio and television documentaries is currently ongoing. A pre‐knowledge survey was conducted to determine the baseline status as regards knowledge and awareness levels on IAS in the project area. A post‐knowledge survey will be conducted at a subsequent stage to assess the effectiveness of the project interventions on public awareness and knowledge levels. Recommendations for future action are also presented.  相似文献   

Mount St. Elias     

Dall WH 《Science (New York, N.Y.)》1890,16(408):303-304

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Gin einfaches Rivellirinftrument     

Fürftl. Hohenzollern’fcher Revier-Bermalter Jofeph Hurth 《European Journal of Forest Research》1868,12(1):296-298

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