Isolation of Mycoplasma mycoides from outbreaks of caprine mycoplasmosis in Brazil     

E R Nascimento  M da G Nascimento  E A Freundt  H Andersen 《The British veterinary journal》1986,142(3):246-257

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Estudio micologico del aparato respiratorio de Gallus gallus     

Aída Cubillos  Horacio Prüssing 《Zoonoses and public health》1979,26(8):605-613

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Flow cytometric assay in peripheral blood of dogs--reference values for leukocytes from Brazilian beagles     

Fujiwara RT  Bueno LL  Vale AM  Franca-Silva JC  da Costa RT  Quetz Jda S  Machado-Coelho GL  Reis AB  Martins Filho OA  Genaro O  Nascimento E  Mayrink W 《Polish journal of veterinary sciences》2005,8(1):17-22

Use of domestic reference values in the flow cytometry analysis is known to improve its accuracy by integrating local variations as gender, race and age. Up to date application of flow cytometry in veterinary medicine has been limited to describe the percentual values just for peripheral lymphocytes subsets of blood. We now report establishment of reference values for a wide range of proportional and absolute numbers of peripheral blood leukocytes, including T cells subsets, B cells, monocytes and eosinophils, applicable to the healthy population of Beagles in Brazil and other regions with similar demographic characteristics. Normal reference values were also established to estimate the gender-related differences. This information will provide clinical aid in the evaluation of immunologic status as well as standard values for experimental animals of dogs from Brazil and other similar regions.  相似文献   

Detection of the enterotoxins A, B, and C genes in Staphylococcus aureus from goat and bovine mastitis in Brazilian dairy herds   总被引：2，自引：0，他引：2  

da Silva ER  do Carmo LS  da Silva N 《Veterinary microbiology》2005,106(1-2):103-107

To determine the distribution of genes that encode enterotoxins A, B and C, 36 strains of Staphylococcus aureus isolated from goat mastitis and 64 isolated from bovine mastitis were analyzed by Multiplex PCR. Of the total strains studied, 37 (37%) were detected to have some of the SEs genes. From the bovine mastitis strains, 4 (6.3%) co-amplified the sea and seb genes and 2 (3.1%) were positive for the sec gene. From the goat mastitis strains, 31 (86%) tested positive to the Multiplex, and the sec gene was detected in all of them. The production of SE was detected in all strains harboring the corresponding gene. The results demonstrated that S. aureus isolated from goat mastitis had a higher enterotoxigenic potential than those isolated from bovine mastitis. Additionally, the presence of the sec gene in the majority of goat mastitis strains suggests a possible involvement of SEC in goat mastitis pathogenesis.  相似文献   

Prevalence of equine infectious anaemia (swamp fever) in Guyana     

O Bamigboye  R M da Silva 《The British veterinary journal》1981,137(5):538-540

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Infection due to Mammomonogamus laryngeus in the buffalo (Bubalus bubalis) in Para State, Brazil.     

N M da Serra Freire 《Tropical animal health and production》1979,11(2):69-70

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Cervical syringohydromyelia secondary to a brainstem tumor in a dog     

da Costa RC  Parent JM  Poma R  Duque MC 《Journal of the American Veterinary Medical Association》2004,225(7):1061-4, 1048

An 11-year-old male Pekingese was evaluated because of a history of head tilt. Neurologic examination revealed a right-sided head tilt, ataxia, scoliosis, and proprioceptive deficits. Diagnostic testing included magnetic resonance imaging (MRI) of the head and neck. After IV administration of gadopentetate dimeglumine, an extra-axial, highly contrast-enhanced mass in the brainstem, cerebellar herniation, and syringohydromyelia were detected via MRI. The dog was treated with corticosteroids and radiation therapy of the mass for 4 weeks (total dose, 42.5 Gy). Magnetic resonance imaging was repeated 9 weeks and 6 months after radiation therapy; compared with the initial findings, a reduction in the size of the brainstem mass was observed in both MRI scans. The third MRI scan also revealed a normal cerebellar shape, no evidence of herniation, and resolution of syringohydromyelia in the dog at that time. It is recommended that whenever syringohydromyelia is observed via MRI, a primary cause (cranial or caudal to the affected region) should be sought.  相似文献   

Cervical diskospondylitis in a calf: clinical, radiographic, and necroscopy findings          下载免费PDF全文

Paiva Della Libera AM  Leal ML  Gregory L  Rodrigues da Silva DY  Unruh SM  Birgel Júnior EH  Benesi FJ 《The Canadian veterinary journal. La revue veterinaire canadienne》2004,45(8):700-701

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Preservation of cadavers for surgical technique training     

Guimarães da Silva RM  Matera JM  Ribeiro AA 《Veterinary surgery : VS》2004,33(6):606-608

OBJECTIVE: To evaluate a technique for preservation of organoleptic tissue characteristics (color, odor, texture, and flexibility) in cadavers used for surgical instruction. STUDY DESIGN: Experimental study. ANIMALS: Forty-three canine cadavers. METHODS: Cadavers were preserved with a modified Larssen solution of the Hospital Cochim, Paris and cryopreservation. Tissue handling qualities were evaluated in surgical laboratory sessions. RESULTS: All cadavers kept texture and tissues consistency, especially skin and muscle, similar to those of live animals. Some skin desquamation and pallor of the mucous membranes occurred with repetitive freeze-thaw cycles. CONCLUSIONS: This preservation technique provides acceptable cadaver quality and tissue handling for use in surgical instruction. CLINICAL RELEVANCE: Preparation of patient cadavers by intravascular injection of modified Larssen solution yielded suitable instructional models for surgical training.  相似文献   

Enrofloxacin assay validation and pharmacokinetics following a single oral dose in chickens     

da Silva RG  Reyes FG  Sartori JR  Rath S 《Journal of veterinary pharmacology and therapeutics》2006,29(5):365-372

The pharmacokinetics of enrofloxacin (ENRO), a fluoroquinolone antimicrobial agent, was studied in male broiler chickens (Cobb) after single oral administration of 10 mg of ENRO/kg b.w. A high-performance liquid chromatography-photodiode array detector (DAD) (HPLC-DAD) method was developed and validated and used for quantitation of ENRO and its major metabolite ciprofloxacin in plasma. The HPLC analyses were carried out using a cationic-octadecyl mixed column and 0.05 mol/L phosphate buffer (pH 2.5)/acetonitrile as mobile phase. The sample preparation of plasma consisted of the precipitation of proteins followed by solid phase extraction on cationic-octadecyl mixed cartridges. The method was validated considering linear range, linearity, selectivity, sensitivity, limit of detection (LOD), limit of quantitation (LOQ), intra- and inter-day precisions and accuracy. The LOD and LOQ for both fluoroquinolones were 60 and 200 ng/mL for plasma. The plasma concentration vs. time graph was characteristic of a two-compartment open model. The maximal plasma concentration of 1.5 +/- 0.2 mg/mL was achieved at 9 +/- 2 h. The elimination half-life and the mean residence time of ENRO were 1.5 +/- 0.2 and 15.64 h, respectively. The area under the concentration-time curve was calculated as 35 +/- 4 mgxh/mL.  相似文献