犬瘟热病毒的静水压灭活   总被引：4，自引：1，他引：3  

池元斌  夏咸柱  王雪梅  余春  王琰第  何洪彬  范泉水  黄耕  王允海  刘海涛  张春红 《中国兽医学报》2001,21(1):35-37

以250MPa的静水压力对犬瘟热病毒（CDV）处理30min之后，CDV被灭活，由其制成的灭活疫苗诱导犬产生中和抗体的能力明显优于传统的福尔马林灭活疫苗，免疫保护率达90％。电镜观察显示，经不同程度静水压力处理后的CDV粒子均发生变形，表面呈现凸起，但CDV H基因部分片段的RT－PCR扩增结果揭示，高达510MPa的静水压力作用30min也未能使其基因片段受到破坏。  相似文献   

Investigation of Cervical Patency and Uterine Appearance in Domestic Cats by Fluoroscopy and Scintigraphy     

K Chatdarong  N Kampa  E Axnr  C Linde‐Forsberg 《Reproduction in domestic animals》2002,37(5):275-281

The cervical patency of six domestic female cats was monitored under sedation by infusion of contrast medium (Omnipaque) into the cranial vagina during early oestrus, mid‐oestrus, late oestrus and interoestrus or a radiopharmaceutical (^99mTc‐HSA) during mid‐ and interoestrus in a non‐ovulatory oestrous cycle. The transport of the contrast medium or the radiopharmaceutical through the cervix and within the uterine horns was observed under fluoroscopy and with the aid of scintigraphy. In three of the queens, transcervical transport of contrast medium was demonstrated in all stages of oestrus, in one queen during mid‐oestrus, late oestrus and 1 day after oestrus, and in two queens only during late oestrus. The relations between the cervical patency to the contrast medium and the oestrous behaviour, cornification of the vaginal cells and the serum oestradiol‐17β concentration were evaluated, and a relationship was found between the cervical patency and the degree of vaginal cornification. Transcervical transport of the radiopharmaceutical was observed in three queens during mid‐oestrus. When the cervix was open, hysterography under a fluoroscope and hysteroscintigraphy were performed. The fluoroscopic and scintigraphic recordings revealed the patterns of the uterine contractions during oestrus in both ascending and descending directions, and the movement of the uterine contents back and forth between the uterine horns. The hysterograms were classified according to the shape of the uterine horns and the appearance of the endometrial lining. Spiral‐shaped uterine horns with a smooth inner contour were observed in two queens, and a corkscrew appearance with irregular filling defects in the uterine lumen was shown in two queens that had developed subclinical cystic endometrial hyperplasia. These findings demonstrated that fluids or particles deposited in the cranial vagina of the cat can be transported into the uterus during some stages of the oestrous cycle. The fluoroscopic and scintigraphic techniques developed in this study may be further modified to permit more detailed studies of uterine contractile patterns and sperm transport in the feline female reproductive tract. Hysterography proved useful to diagnose uterine disease. The information on cervical patency is of value also for the development of techniques for artificial insemination in this species, and should be studied also in the ovulatory cycle.  相似文献   

全沟硬蜱围食膜初步观察研究     

朱振勤  夏明仪  陈季武  夏晶  傅蓓蓓 《中国兽医寄生虫病》2002,10(4):1-4

本工作用光镜和电镜技术对全沟硬蜱的围食膜进行了初步观察研究。结果表明，饥饿幼虫、若虫、成虫以及吸血期雄性成虫不具围食膜。吸血期幼虫、若虫及雌性成虫最晚分别于吸血开始后20、20及18小时出现了单层结构的围食膜；缓慢吸血期围食膜的厚度持续增加，而在快速吸血期其厚度却会显著减小。饱血后发育期围食膜不断增厚，愈来愈拱曲，与肠壁的距离不断扩大，并形成多层结构；至少分别于饱血后12、25及11天，仍能在幼虫、若虫及雌性成虫肠腔观察到完好状态的围食膜。全沟硬蜱是我国北方地区多种血液传播病原体的重要媒介，其围食膜的发现及围食膜形态变化的初步描述，对进一步认识这些病原体在全沟硬蜱体内迁移扩散规律、发育动态以及向宿主的传播途径，可提供有益的启示。  相似文献   

一种估测亚麻出麻率的简便方法     

李明  杨学 《中国麻业科学》2001,23(1):9-10

统计分析显示亚麻茎中段的出麻率与单株出麻率相关极显著，可以在育种的单株选择过程中，利用茎中段的出麻率来快速估算单株出麻率，以减轻工作量，提高工作效率。  相似文献   

传染性法氏囊病疫苗免疫母鸡后子代雏鸡外周血液的B细胞和免疫球蛋白含量变化   总被引：2，自引：1，他引：1  

葛铭  张瑞莉  郑世民 《黑龙江畜牧兽医》2001,(3):7-8

应用免疫SPA菌体花环法和间接ELISE法对传染性法氏囊病（IBD）疫苗免疫母鸡后，其子代雏鸡外周血液B细胞数量和IgG、IgM、IgA含量进行了动态研究。结果发现：IBD疫苗免疫后，其子代雏鸡外周血液B细胞数量和IgG、IgM、IgA含量均不同程度地高于未免疫的相应对照雏鸡，表明IBD疫苗免疫母鸡后，其子代雏鸡外周血液的体液免疫功能明显增强。而传染性法氏囊病超强毒株攻击子代雏鸡后，未免疫子代雏鸡外周血液的上述各项指标均明显低于疫苗免疫的子代雏鸡，这与IBDV感染雏鸡后，其体液免疫中枢器官法氏囊严重损害，淋巴细胞变性坏死等有关，也是导致感染雏鸡免疫抑制的基础。  相似文献   

冰川棘豆的主要饲料营养成分分析   总被引：9，自引：0，他引：9  

谭远友  王建华 《中国草食动物》2001,3(3):37-38

测得冰川棘豆干样中干物质，粗蛋白，粗脂肪，粗纤维和粗灰分的含量分别为93．54％，14．18％，3．32％，25．10％和12．93％。  相似文献   

Reproductive roles predict sexual dimorphism in internal and external morphology of lake whitefish,Coregonus clupeaformis     

S. J. Casselman  A. I. Schulte‐Hostedde 《Ecology of Freshwater Fish》2004,13(3):217-222

Abstract – The different reproductive roles of the sexes can predict the direction and magnitude of sexual dimorphism of external and internal morphology. Males should have enlarged structures that enhance the acquisition of mating opportunities, whereas females are predicted to have enlarged organs that are associated with the production of eggs. We tested these predictions in male and female lake whitefish, a species in which both sexes have similar overall body size and shape. After controlling for body size, male lake whitefish had significantly longer jaws and pectoral and pelvic fins, larger hearts, and more muscle than females. Sexual dimorphism in relative muscle mass may be one of the most fundamental morphological differences between males and females. Females had relatively heavier livers than males. Because the liver is important for the breakdown of fats and vitellogenesis, selection should favour an enlarged liver in females for the processing of energy and the production of large numbers of eggs.  相似文献   

PCR扩增TK基因检测鸡传染性喉气管炎病毒的研究   总被引：3，自引：0，他引：3  

彭大新  甘军纪  高崧  吴艳涛  刘金彪 《动物医学进展》2001,22(3):59-61

根据已发表序列设计一对包含鸡传染性喉气管炎病毒（ILTV）TK基因全长核苷酸的1259bp引物，对2株ILTV强毒和1株ILTV疫苗毒进行PCR扩增，均能扩增出预期大小的目的片段，酶切分析证实了目的片段的特异性，而其它禽病原体的扩增均为阴性。PCR检测ILTV DNA的最小检测量为75pg。此方法检测人工接种鸡的气管棉拭样品，均能检测到ILTV，因此可用于临诊样品鸡传染性喉气管炎病的检测和诊断。  相似文献   

我国住宅现代化生产相关问题研究     

杨明  曹峰军  金虹 《林业科技情报》2002,34(2):21-22

本文从我国住宅产业发展现状入手,针对我国住宅现代化生产相关问题进行分析和研究,提出住宅产业现代化的是解决住房矛盾的前提、模数协调是住宅现代化生产的技术基础、认证制度是住宅现代化生产的有力保证、住宅性能评价体系是住宅现代化生产的重要内容的观点,对当前形势下我国住宅产业的发展方向具有一定的指导意义。  相似文献   

甜瓜种质资源的判别分析   总被引：6，自引：0，他引：6  

张鲁刚  王鸣 《园艺学报》1992,19(1):35-40

  相似文献