Real-time NASBA for Detection of Strawberry Vein Banding Virus     

D. Vašková  J. Špak  M.M. Klerks  C.D. Schoen  J.R. Thompson  W. Jelkmann 《European journal of plant pathology / European Foundation for Plant Pathology》2004,110(2):213-221

An assay for the detection of Strawberry vein banding virus (SVBV) in Fragaria spp. based on nucleic acid sequence based amplification (NASBA) and real-time detection using molecular beacons (real-time NASBA) is described. This assay was compared both with biological indexing, the current method for certification of SVBV, and a newly optimised PCR-based detection method. Performance of the assay was tested on three SVBV isolates in Fragaria indicator plants and 317 field strawberries from five European countries. The assay was shown to be SVBV-specific, testing negative for other common aphid-borne strawberry viruses. The virus was detected in purified total RNA preparations diluted a millionfold, which is an amount equivalent to 1ng of fresh material. The real-time NASBA method developed here offers the potential of a fast, sensitive and reliable approach for the routine diagnosis of strawberry stock material.  相似文献   

A Natural Population of Recombinant Plum Pox Virus is Viable and Competitive under Field Conditions     

Miroslav Glasa  Véronique Marie-Jeanne  Gérard Labonne  Zdeno Šubr  Otakar Kúdela  Jean-Bernard Quiot 《European journal of plant pathology / European Foundation for Plant Pathology》2002,108(9):843-853

The isolate BOR-3, collected in Slovakia in 1996, was recently identified as a natural recombinant between an M and D type of Plum pox virus (PPV). Biological assays demonstrated its capacity to be aphid- and graft-transmitted to various Prunus spp. hosts. A study was carried out to determine the further presence of PPV recombinants in two epidemiologically distinct areas – Slovakia and France. Tools based on PPV-M and D subgroup typing, targeting P3–6K₁, CI and CP regions of the PPV genome were used for recombinant identification. Closely related recombinant variants were detected in different Prunus spp. during a survey conducted in Slovakia in 2001, but not within a set of selected PPV isolates from France collected between 1985 and 2001. Sequence analysis of the (Cter)NIb–(Nter)CP region of 10 recombinant isolates from Slovakia showed their high homology, reaching more than 98%. All the recombinant isolates shared the same recombination breakpoint situated in the C terminus of the NIb gene. Our study demonstrates that the PPV recombinants are viable and competitive with conventional PPV-M and D isolates. The present work indicates that the occurrence of recombinants within PPV isolates might be more common than previously assumed.  相似文献   

Position of the head is not associated with changes in horse vision     

L. BARTOŠ  J. BARTOŠOVÁ  L. STAROSTOVÁ 《Equine veterinary journal》2008,40(6):599-601

It has become accepted that the horse cannot see directly in front when the nose is lowered and must therefore rely on the rider. We tested the hypothesis that this conclusion would be correct only if the horse did not adjust the eyeball horizontal axis to changes of the head position. The results of the present study suggest that it is unlikely that horses have limited vision in relation to their head position when driven by the rider, and that the horse maintains the optimal horizontal eyeball position regardless of head position relative to the ground.  相似文献   

Monitoring of the Roundup Ready soybean in the Vojvodina province in Serbia     

Zorica Nikolić  Ksenija Taški-Ajduković  Mladen Tatić  Svetlana Balešević-Tubić 《Industrial Crops and Products》2009,29(2-3):638-641

The Vojvodina province is the most important agricultural area of the Republic of Serbia and is its largest soybean producer. Serbian low forbids the introduction of genetically modified organisms (GMO) into the environment and demands labeling of food containing more than 0.9% GMO. This aim of this study is to monitor the Roundup Ready (RR) soybean in fields and in food products using PCR and immunoassays. A duplex PCR reaction was performed to analyze the lectin gene and 35S promoter using DNA isolated from soybean leaf and seed sample. Specific detection of RR markers was performed using nested PCR and verified by immunoassay. RR soybeans were found in 68 fields in samples from 2003 and 28 fields in 2004. The presence of the RR soybean was confirmed in 44 seed samples out of 7142 samples examined in 2006 and in 108 out of 7171 samples examined in 2007. The data presented here are the first to demonstrate the presence of RR soybean in the Serbia fields.  相似文献   

Quercetin influences response in Nicotiana megalosiphon infected by satellite-associated cucumber mosaic virus     

Rusak  Gordana  Krajačićl  Mladen  Krsnik-Rasol  Marijana  Gutzeit  Herwig O. 《植物病害和植物保护杂志》2007,114(4):145-150

Journal of Plant Diseases and Protection - Quercetin is a prominent flavonoid with a remarkable spectrum of biochemical activities. Its impact on human health, as well as its role in the normal...  相似文献   

The introns in FLOWERING LOCUS T-LIKE (FTL) genes are useful markers for tracking paternity in tetraploid Chenopodium quinoa Willd.     

Helena Štorchová  Jana Drabešová  David Cháb  Jan Kolář  Eric N. Jellen 《Genetic Resources and Crop Evolution》2015,62(6):913-925

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Effect of soil CO2 concentration on microbial biomass   总被引：1，自引：0，他引：1  

H. Šantrůčková  M. Šimek 《Biology and Fertility of Soils》1997,25(3):269-273

The effect of increasing soil CO₂ concentration was studied in six different soils. The soils were incubated in ambient air (0.05 vol.% CO₂) or in air enriched with CO₂ (up to 5.0 vol.% CO₂). Carbon dioxide evolution, microbial biomass, growth or death rate quotients and glucose decay rate were measured at 6, 12 and 24 h of CO₂ exposure. The decrease in soil respiration ranged from 7% to 78% and was followed by a decrease in microbial biomass by 10–60% in most cases. High CO₂ treatments did not affect glucose decay rate but the portion of C_gluc mineralized to CO₂ was lowered and a larger portion of C_gluc remained in soils. This carbon was not utilized by soil microorganisms. Received: 30 August 1996  相似文献   

Complete or incomplete dominance of resistance of the oat cultivar Delphin as a function of crown rust culture     

J. Šebesta 《Euphytica》1979,28(3):807-809

Summary In oat cv. Delphin (Bonda x Carstens Vii) two complementary genes were found conferring resistance to sixteen cultures of ten crown rust (Puccinia coronata Cda. var. avenae Fraser ET Led.) races. The complementary genes showed full dominance to eight races and incomplete dominance to two races.  相似文献   

Properties and bioavailability of particulate and mineral‐associated organic matter in Arctic permafrost soils,Lower Kolyma Region,Russia          下载免费PDF全文

N. Gentsch  R. Mikutta  O. Shibistova  B. Wild  J. Schnecker  A. Richter  T. Urich  A. Gittel  H. Šantrůčková  J. Bárta  N. Lashchinskiy  C. W. Mueller  R. Fuß  G. Guggenberger 《European Journal of Soil Science》2015,66(4):722-734

Permafrost degradation may cause strong feedbacks of arctic ecosystems to global warming, but this will depend on if, and to what extent, organic matter (OM) is protected against biodegradation by mechanisms other than freezing and anoxia. Here, we report on the amount, chemical composition and bioavailability of particulate (POM) and mineral‐associated OM (MOM) in permafrost soils of the East Siberian Arctic. The average total organic carbon (OC) stock across all soils was 24.0 ± 6.7 kg m^?2 within 100 cm soil depth. Density fractionation (density cut‐off 1.6 g cm^?3) revealed that 54 ± 16% of the total soil OC and 64 ± 18% of OC in subsoil horizons was bound to minerals. As well as sorption of OM to clay‐sized minerals (R² = 0.80; P < 0.01), co‐precipitation of OM with hydrolyzable metals may also transfer carbon into the mineral‐bound fraction. Carbon:nitrogen ratios, stable carbon and nitrogen isotopes, ¹³C‐NMR and X‐ray photoelectron spectroscopy showed that OM is transformed in permafrost soils, which is a prerequisite for the formation of mineral‐organic associations. Mineral‐associated OM in deeper soil was enriched in ¹³C and ¹⁵N, and had narrow C:N and large alkyl C:(O‐/N‐alkyl C) ratios, indicating an advanced stage of decomposition. Despite being up to several thousands of years old, when incubated under favourable conditions (60% water‐holding capacity, 15°C, adequate nutrients, 90 days), only 1.5–5% of the mineral‐associated OC was released as CO₂. In the topsoils, POM had the largest mineralization but was even less bioavailable than the MOM in subsoil horizons. Our results suggest that the formation of mineral‐organic associations acts as an important additional factor in the stabilization of OM in permafrost soils. Although the majority of MOM was not prone to decomposition under favourable conditions, mineral‐organic associations host a readily accessible carbon fraction, which may actively participate in ecosystem carbon exchange.  相似文献   

High prevalence of metabolic syndrome in an elderly Croatian population - a multicentre study     

Pašalić D  Dodig S  Corović N  Pizent A  Jurasović J  Pavlović M 《Public health nutrition》2011,14(9):1650-1657

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