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951.
Diversity analysis was performed among 39 cultivated lentil (Lens culinaris Medik.) accessions of Central Asia and Caucasian origin using five highly polymorphic microsatellite markers. A total of 33 alleles determined ranging from 3 to 8 per locus. Estimated gene diversity value for 33 loci was 0.66. Genetic similarity indices among 39 accessions ranged from 0.24 to 1.0. Cluster analysis using the unweighted pair group method with arithmetic mean method classified accessions into six major groups at 0.5 similarity coefficient. More than half accessions from Tajikistan formed large cluster. On the other hand, a few accessions from each country showed unique genotypes. Overall, most of the accessions, except ones with closely related origin, were distinguished by the present high quality DNA fingerprinting. This molecular diversity information gives important basis for conservation strategy in gene bank and exotic germplasm introduction in breeding programs in Central Asia and Caucasian countries.  相似文献   
952.
Urea fertilizer‐induced N2O emissions from soils might be reduced by the addition of urease and nitrification inhibitors. Here, we investigated the effect of urea granule (2–3 mm) added with a new urease inhibitor, a nitrification inhibitor, and with a combined urease inhibitor and nitrification inhibitor on N2O emissions. For comparison, the urea granules supplied with or without inhibitors were also used to prepare corresponding supergranules. The pot experiments without vegetation were conducted with a loess soil at (20 ± 2)°C and 67% water‐filled pore space. Urea was added at a dose of 86 kg N ha–1 by surface application, by soil mixing of prills (<1 mm) and granules, and by point‐placement of supergranules (10 mm) at 5 cm soil depth. A second experiment was conducted with spring wheat grown for 70 d in a greenhouse. The second experiment included the application of urea prills and granules mixed with soil, the point‐placement of supergranules and the addition of the urease inhibitor, and the combined urease plus nitrification inhibitors at 88 kg N ha–1. In both experiments, maximum emissions of N2O appeared within 2 weeks after fertilization. In the pot experiments, N2O emissions after surface application of urea were less (0.45% to 0.48% of total fertilization) than from the application followed by mixing of the soil (0.54% to 1.14%). The N2O emissions from the point‐placed‐supergranule treatment amounted to 0.64% of total fertilization. In the pot experiment, the addition of the combined urease plus nitrification inhibitors, nitrification inhibitor, and urease inhibitor reduced N2O emissions by 79% to 87%, 81% to 83%, and 15% to 46%, respectively, at any size of urea application. Also, the N2O emissions from the surface application of the urease‐inhibitor treatment exceeded those of the granules mixed with soil and the point‐placed‐supergranule treatments receiving no inhibitors by 32% to 40%. In the wheat growth experiment, the N2O losses were generally smaller, ranging from 0.16% to 0.27% of the total fertilization, than in the pot experiment, and the application of the urease inhibitor and the combined urease plus nitrification inhibitors decreased N2O emissions by 23% to 59%. The point‐placed urea supergranule without inhibitors delayed N2O emissions up to 7 weeks but resulted in slightly higher emissions than application of the urease inhibitor and the urease plus nitrification inhibitors under cropped conditions. Our results imply that the application of urea fertilizer added with the combined urease and nitrification inhibitors can substantially reduce N2O emissions.  相似文献   
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955.

Purpose

Chickpea is generally cultivated after seed treatment with host-specific Mesorhizobium ciceri, the nitrogen-fixing bacterium forming root nodules. Some species of free-living cyanobacteria are capable of nitrogen fixation. We examined the rhizosphere microbiota changes and the potential for plant growth promotion by applying a free-living, nitrogen-fixing cyanobacterium and the biofilm formulation of cyanobacterium with M. ciceri, relative to M. ciceri applied singly, to two each of desi and kabuli varieties of chickpea.

Materials and methods

Denaturing gradient gel electrophoresis (DGGE) profiles of archaeal, bacterial and cyanobacterial communities and those of phospholipid fatty acids (PLFAs) were obtained to evaluate the changes of the microbial communities in the chickpea rhizosphere. Plant growth attributes, including the pod yields and the availabilities of soil macronutrients and micronutrients, were monitored.

Results and discussion

The DGGE profiles showed distinct and characteristic changes due to the microbial inoculation; varietal differences exerted a marked influence on the archaeal and cyanobacterial communities. However, bacterial communities were modulated more by the type of microbial inoculants. Abundance of Gram-negative bacteria (in terms of notional PLFAs) differed between the desi and the kabuli varieties inoculated with M. ciceri alone, and the principal component analysis of PLFA profiles confirmed the characteristic effect of microbial inoculants tested. Microbial inoculation led to increases in the 100-seed weight and differential effects on the concentrations of available nitrogen and phosphorus, and those of iron, zinc and copper, suggesting their increased cycling in the rhizosphere.

Conclusions

Microbial inoculation of chickpea brought out the characteristic changes in rhizosphere microbiota. Consequently, the growth promotion of chickpea and nutrient cycling in its rhizosphere distinctively differed. Further studies are needed to analyse the association and dynamic changes in the microbial communities to define the subset of microorganisms selected by chickpea in its rhizosphere and the influence of microbial inoculation.
  相似文献   
956.
Forests are among the most important carbon sinks on earth. However, their complex structure and vast areas preclude accurate estimation of forest carbon stocks. Data sets from forest monitoring using advanced satellite imagery are now used in international policy agreements. Data sets enable tracking of emissions of CO2 into the atmosphere caused by deforestation and other types of land-use changes. The aim of this study is to determine the capability of SPOT-HRG Satellite data to estimate aboveground carbon stock in a district of Darabkola research and training forest, Iran. Preprocessing to eliminate or reduce geometric error and atmospheric error were performed on the images. Using cluster sampling, 165 sample plots were taken. Of 165 plots, 81 were in natural habitats, and 84 were in forest plantations. Following the collection of ground data, biomass and carbon stocks were quantified for the sample plots on a per hectare basis. Nonparametric regression models such as support vector regression were used for modeling purposes with different kernels including linear, sigmoid, polynomial, and radial basis function. The results showed that a third-degree polynomial was the best model for the entire studied areas having an root mean square error, bias and accuracy, respectively, of 38.41, 5.31, and 62.2; 42.77, 16.58, and 57.3% for the best polynomial for natural forest; and 44.71, 2.31, and 64.3% for afforestation. Overall, these results indicate that SPOT-HRG satellite data and support vector machines are useful for estimating aboveground carbon stock.  相似文献   
957.
Water, Air, & Soil Pollution - Sustainable groundwater quality has become a major concern for the agro-based country like Bangladesh. Integrated approaches of various irrigation water quality...  相似文献   
958.
Nitrification inhibitory properties of plant substances offer potential benefits for agricultural applications. In this study, hexane-extracted seed oil, and 10, 20, and 30 mg/pot dry powder of seed exocarp and mesocarp from Acrocomia totai were applied in a quick nitrification bioassay for 24 or 50 hr, as well as for 4 weeks laboratory soil incubation, to investigate their effects on nitrification process. Results showed that applied oil concentrations significantly reduced nitrification rates. The extent of inhibition was increased with the oil concentrations from 5 to 25 µl in both experiments. The exocarp and mesocarp powders also inhibited nitrification, but it was not as high as oil-induced inhibition. Analysis of oil fatty acids showed that oil consisted of 71%, 26%, and 3% of saturated, monounsaturated, and polyunsaturated fatty acids, respectively. This suggests that fatty acids or some unknown ingredients in crude oil could be the responsible inhibiting compound.  相似文献   
959.
Iron (Fe) nanoparticles (NPs), with 30–40 nm diameter, were stabilized on sand. The resulting synthesized Fe/SiO2 NPs, with different Fe contents (0–25 mg kg?1) were employed as fertilizers in probing the mean germination time (MGT), growth and dry matter of barley and maize and their comparison with common Fe/SiO2 in a completely randomized design (CRD) experiment. The results showed that our fertilizers had significant effects on MGT, with the lowest of 0.58 day for barley and 0.79 day for maize; at 15 and 5 mg kg?1 nano Fe/SiO2, respectively. Application of 15 mg kg?1 of nano Fe/SiO2 increased the shoot length: 8.25% and 20.8% for barley and maize, respectively. However, the concentration of 25 mg kg?1 had a negative impact on shoot length in barley. Increasing the concentrations of both nano and common Fe/SiO2 particles, increased the root lengths in both plants, however this increase was higher with the application of nano Fe/SiO2. Likewise, seedling length enlarged with the concentration increase of both Fe/SiO2 particles and was more pronounced with nano Fe/SiO2. The application of nano Fe/SiO2 was more effective compared with the common Fe/SiO2 in encouraging barley and maize growth. The positive impact was higher in maize than barley.  相似文献   
960.
Soil contaminated by chromium (Cr) is a major concern for sustainable agriculture. Considering this as a basis, the present study was designed to isolate Cr(VI)-reducing and plant growth-promoting bacterial strain from contaminated sampling sources. In this study, Rhizobium strain ND2 was isolated from the root nodules of Phaseolus vulgaris grown in leather industrial effluent contaminated soil. The strain ND2 exhibited strong resistance to different heavy metals and reduced 30 and 50 µg ml?1 concentrations of Cr(VI) completely after 80 and 120 h of incubation, respectively, as well as chromium adsorption and immobilization were confirmed by scanning electron microscopic equipped with energy X-ray spectroscopy. In addition, the strain produced 21.73 and 36.86 µg ml?1 of indole-3-acetic acid at 50 and 100 µg ml?1 of L-tryptophan supplimentations, respectively. Strain ND2 positively affected the exo-polysaccharide, ammonia, protease and catalase production and stimulated root length of various test crops under Cr(VI) stress. Moreover, Rhizobium strain ND2 has the potential to colonize the diverse agricultural crops. Thus, the present findings strongly suggested that the multipotential properties of ND2 could be exploited for bioremediation of contaminated sites with Cr(VI) as well as potential bio fertilizer for enhancing the agricultural productivity.  相似文献   
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