Stable integration and expression of a cry1Ia gene conferring resistance to fall armyworm and boll weevil in cotton plants          下载免费PDF全文

Carliane RC Silva  Rose Monnerat  Liziane M Lima  Érica S Martins  Péricles A Melo Filho  Morganna PN Pinheiro  Roseane C Santos 《Pest management science》2016,72(8):1549-1557

  相似文献   

Economical and financial analysis of lamb finishing fed with diets formulated according to the NRC (1985) and the NRC (2007)     

Marcos Cláudio Pinheiro Rogério  Eliane Minervina de Castro  Espedito Cezário Martins  Jomar Patrício Monteiro  Kleibe de Moraes Silva  Magno José Duarte Cândido  Tereza Cristina Lacerda Gomes  Antoine Francis Roux Bloc  Ângela Maria de Vasconcelos  Eneas Reis Leite  Hélio Henrique Araújo Costa 《Tropical animal health and production》2012,45(1):259-266

This study compares both versions of the nutritional requirement system determined by the National Research Council (NRC) version 1985 (NRC85) and NRC version 2007 (NRC07), for finishing lambs in feedlots. Nineteen crossbred lambs were divided in four groups representing four experimental treatments: one diet according to NRC85 and three diets according to NRC07. The diets recommended by NRC07 considers crude protein intake relative to ruminal undegradable protein at 20, 40, and 60 % levels (NRC07/20, NRC07/40, and NRC07/60). Diets were composed of Brazilian semi-arid native grass silage, soybean meal, corn, annatto byproduct, and limestone. Purchases and sales of lambs were done according to average market prices in Brazil. The economic indicators considered pointed that all treatments were viable but NRC07/20 and NRC07/60 were more profitable with similar net present values (NPVs) and internal return rates (IRRs). NRC07/20 was the best option showing an IRR of 17.20 % and a payback period (PP) of 5.07 considering a fixed annual interest rate of 6 %. Sensitivity analysis considering a 10 % raise in variable costs showed negative NPVs, IRRs inferior to the opportunity cost rates adopted and PPs that exceeded the planning horizon of 7 years for both NRC85 and NRC07/40.  相似文献   

Adapting clonally propagated crops to climatic changes: a global approach for taro (<Emphasis Type="Italic">Colocasia esculenta</Emphasis> (L.) Schott)     

V. Lebot  V. Tuia  A. Ivancic  G. V. H. Jackson  F. Saborio  G. Reyes  S. Rodriguez  G. Robin  R. Traoré  L. Aboagye  J. Onyeka  W. van Rensburg  V. Andrianavalona  A. Mukherjee  M. S. Prana  D. Ferraren  B. Komolong  F. Lawac  S. Winter  M. A. A. Pinheiro de Carvalho  T. Iosefa 《Genetic Resources and Crop Evolution》2018,65(2):591-606

Clonally propagated crop species are less adaptable to environmental changes than those propagating sexually. DNA studies have shown that in all countries where taro (Colocasia esculenta (L.) Schott) has been introduced clonally its genetic base is narrow. As genetic variation is the most important source of adaptive potential, it appears interesting to attempt to increase genetic and phenotypic diversity to strengthen smallholders’ capacity to adapt to climatic changes. A global experiment, involving 14 countries from America, Africa, Asia and the Pacific was conducted to test this approach. Every country received a set of 50 indexed genotypes in vitro assembling significant genetic diversity. After on-station agronomic evaluation trials, the best genotypes were distributed to farmers for participatory on-farm evaluation. Results indicated that hybrids tolerant to taro leaf blight (TLB, Phytophthora colocasiae Raciborski), developed by Hawaii, Papua New Guinea and Samoa breeding programmes outperformed local cultivars in most locations. However, several elite cultivars from SE Asia, also tolerant to TLB, outperformed improved hybrids in four countries and in one country none of the introductions performed better than the local cultivars. Introduced genotypes were successfully crossed (controlled crossing) with local cultivars and new hybrids were produced. For the first time in the history of Aroids research, seeds were exchanged internationally injecting tremendous allelic diversity in different countries. If climatic changes are going to cause the problems envisaged, then breeding crops with wide genetic diversity appears to be an appropriate approach to overcome the disasters that will otherwise ensue.  相似文献   

Serum testosterone,sperm quality,cytological, physicochemical and biochemical characteristics of the prostatic fraction of dogs with prostatomegaly          下载免费PDF全文

A Aquino‐Cortez  BQ Pinheiro  HVR Silva  DBC Lima  TFP Silva  MB Souza  DA Viana  FAF Xavier Júnior  JSAM Evangelista  FZ Brandão  LDM Silva 《Reproduction in domestic animals》2017,52(6):998-1003

Prostatomegaly is a common finding in older non‐neutered dogs. This study compared the serum testosterone, sperm quality and characteristics of the prostatic fraction between healthy dogs and dogs with prostatomegaly. Blood samples of ten dogs (five dogs from each group) were taken for serum testosterone measurement. Sperm motility, vigour, concentration, viability, membrane functionality and morphology were analysed in sperm‐rich fraction. Osmolality, pH, cell types, and albumin, haemoglobin, acid phosphatase, alkaline phosphatase, glucose, triglycerides, cholesterol, calcium, phosphorus, magnesium and chloride were analysed in prostatic fraction. Dogs with prostatomegaly have the lowest sperm motility, vigour, concentration and functional membrane. Dogs with prostatomegaly have the highest glucose, triglycerides and cholesterol. Glucose was the only constituent positively correlated with serum testosterone and prostate volume. It can be concluded that dogs with prostatomegaly have poorer sperm quality, and glucose, triglycerides and cholesterol in prostatic fraction can be used as prostatomegaly biomarkers.  相似文献   

Development of a multiplex RT-PCR assay for simultaneous detection of the major viruses that affect rainbow trout (Oncorhynchus mykiss)     

Ana C. A. S. Pinheiro  Enrico Volpe  Donatella Principi  Santino Prosperi  Sara Ciulli 《Aquaculture International》2016,24(1):115-125

The major viral diseases that affect rainbow trout (Oncorhynchus mykiss) are viral haemorrhagic septicaemia, infectious haematopoietic necrosis, infectious pancreatic necrosis and sleeping disease. In the presented study, we developed a multiplex RT-PCR (mRT-PCR) assay for the simultaneous detection of these four rainbow trout viruses in a single assay. The choice of primers was carried out based on the expected size of the fragments, the temperature and time required for the amplification, and the specificity for the target sequence. Firstly, the method was optimised using reference strains of viral haemorrhagic septicaemia virus (VHSV), infectious haematopoietic necrosis virus (IHNV), infectious pancreatic necrosis virus (IPNV) and sleeping disease virus (SDV) cultivated with permissive cell culture lines; subsequently, the method was used for the identification of these viral infections in rainbow trout samples. Twenty-two samples of rainbow trout, clinically suspected of having viruses, were analysed by the developed method to detect the presence of the four viruses, by directly analysing the animal tissues. The mRT-PCR method was able to efficiently detect the viral RNA in infected cell culture supernatants and in tissue samples, highlighting the presence of single infections as well as co-infections in rainbow trout samples. VHSV/SDV and IHNV/SDV co-infections were demonstrated for the first time in rainbow trout. The mRT-PCR method was revealed to be an accurate and fast method to support traditional diagnostic techniques in the diagnosis of major viral diseases of rainbow trout.  相似文献   

Evaluation of the maize (<Emphasis Type="Italic">Zea mays</Emphasis> L.) diversity on the Archipelago of Madeira     

Miguel Ângelo A. Pinheiro de Carvalho  José Filipe Teixeira Ganança  Ivo Abreu  Nélia F. Sousa  Teresa M. Marques dos Santos  Maria Rita Clemente Vieira  Mario Motto 《Genetic Resources and Crop Evolution》2008,55(2):221-233

  相似文献   

Effects of aflatoxin B 1 on performance and health of tambaqui fingerlings ( Colossoma macropomum )     

Etelvina M. C. G. Nunes  Maria M. G. Pereira  Amilton P. R. Costa  Mar&#;a N. B. A. Araripe  Rodrigo M. Calvet  Carina M. Pereyra  Maria L. X. Azevedo  Raizza E. E. Pinheiro  Verbena C. Alves  Airton M. Conde J&#;nior  Lidiane S. N. Ramos  Joao B. Lopes  Camila G. Marinho  Mar&#;a C. S. Muratori 《International Aquatic Research》2019,11(1):73-83

The detection of mycotoxins in feeds and their ingredients in aquaculture gained prominence due to losses caused in production and animal health, mainly the occurrence of aflatoxin (AFB1). The aim of this study was to evaluate the effects of AFB1 on the performance of tambaqui fingerlings (Colossoma macropomum). Four hundred tambaqui were used. Four different treatments were evaluated: treatment T1, considered as the control treatment (CT) with 3.84 μg kg−1; treatment T2, treatment T3 and treatment T4 with 500, 1000 and 2000 μg kg−1 of AFB1, respectively. The AFB1 of the samples (muscle, liver and kidney) was detected by high-pressure liquid chromatography. Four fingerlings from each treatment for histological analysis were examined. Moreover, the performance parameters (weight gain, feed conversion and feed intake) were studied. The levels of toxins used in T2, T3 and T4 represent a reduction in the growth of 14%, 35% and 45%, respectively. The T3 and T4 showed the lowest weight gain (78%) and the worst feed conversion. Aflatoxin B1 in muscle (3.28 μg kg−1) and kidneys (8.8 μg kg−1) in the T3, as well as liver (4.4 μg kg−1) and kidney (4.08 μg kg−1) in T4, was detected. Histopathological changes in liver and kidney tissues of fingerlings were more pronounced in T3 and T4. Fingerlings that consume feed contaminated with AFB1 in concentrations higher than 500 μg kg−1 present decreases in growth, reduction in weight gain and feed intake with increased feed conversion. The consumption of feed contaminated with 1000 and 2000 μg kg−1 of AFB1 caused severe deterioration in the hepatic and renal tissues.  相似文献   

Tributaries as biodiversity preserves: An ichthyoplankton perspective from the severely impounded Upper Paraná River     

Patricia Sarai da Silva  Leandro Esteban Miranda  Sergio Makrakis  Lucileine de Assumpo  Joo Henrique Pinheiro Dias  Maristela Cavicchioli Makrakis 《水产资源保护:海洋与淡水生态系统》2019,29(2):258-269

Nonlinear regression for description of strawberry (Fragaria x ananassa) production     

Maria Inês Diel  Bruno Giacomini Sari  Dionatan Ketzer Krysczun  Tiago Olivoto  Marcos Vinícius Marques Pinheiro  Daniela Meira 《The Journal of Horticultural Science and Biotechnology》2019,94(2):259-273

The aim of this study was to select growth models to describe strawberry (Fragaria x ananassa)fruit production. To do this, data on 16 treatments (combination of 2 cultivars [Albion and Camarosa], 2 origins [National and Imported], and 4 organic substrates mixed [70% crushed sugar cane residue + 30% organic compost, 70% crushed sugar cane residue + 30% commercial substrate, 70% burnt rice husk + 30% organic compost, and 70% burnt rice husk + 30% commercial substrate]) conducted in a Randomized Complete Block Design (RCBD) with 4 replicates were used. Different parameterizations of the Logistic, Gompertz, and von Bertalanffy models were adjusted for number and weight of fruits per plant (g) accumulated in multiple harvests. The model adjustment and parameter estimation were obtained by ordinary least squares, using a Gauss Newton algorithm. The selection of the best model was chosen by intrinsic and parametric nonlinearity. Among the adjusted nonlinear models, the best adjustment for both variables was achieved by the parameterization 2 of the Logistic model and parameterization 1 of the Gompertz model, because they had lower results with less parametric and intrinsic nonlinearity. However, care should be taken when using the Gompertz model because it tends to overestimate the production estimate and may cause misunderstandings in interpretation.  相似文献   

Genetic variability of high molecular weight glutenin subunits in bread wheat from continental Portugal, Madeira and Canary Islands     

Teresa M. M. dos Santos  Humberto Nóbrega  José Filipe Teixeira Ganan?a  Emanuel Silva  Desire Afonso  Abílio F. M. Gutiérres  Jan J. Slaski  Manhaz Khadem  Miguel ?. A. Pinheiro de Carvalho 《Genetic Resources and Crop Evolution》2012,59(7):1377-1388

The genetic variability of high molecular weight glutenin subunits (HMWGS) composition at the Glu-1 loci in bread wheat (Triticum aestivum L.) was studied electrophoretically using the SDS–PAGE in 3,470 individuals representing 159 populations originated from the Canary Islands (Spain), the Archipelago of Madeira (Portugal) and the continental Portugal. A total of 25 alleles were detected, resulting in 69 different allele combinations. The geographical distribution of the high molecular weight glutenin alleles confirms historical data regarding circulation of wheat germplasm between the Iberian Peninsula and Madeira and between Madeira and the Canary Islands and vice versa.  相似文献