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51.
The Cracidae family of neotropical birds is regarded as one of the most severely threatened in the world. They traditionally have been extensively hunted, and, thus, ex situ efforts for their conservation are recommended and involve the optimization of their care in captivity. Nutrition is a fundamental aspect of husbandry, which influences survival and reproduction in captivity. In this study, a total of 29 animals, including 3 species (Penelope obscura, Penelope superciliaris, and Aburria jacutinga), were subjected to monthly physical examination and blood sampling before and after dietary conversion from the traditional diet of broiler feed, fruits, and vegetables to a nutritionally balanced commercial diet specifically designed for wild Galliformes. The diet change produced differences in several parameters tested, including an increase (P < 0.05) in hemoglobin concentration for all species. Increases (P < 0.05) in erythrocyte count, packed cell volume, and body weight were observed in P. obscura, with a concomitant decrease in the standard deviation for such parameters that show improved uniformity. Globulins and lipase also were reduced (P < 0.05) in P. obscura. Although leukocyte count was lowered and eosinophils were increased in all 3 species after dietary conversion, only these 2 changes were significant (P < 0.05) in P. superciliaris. A. jacutinga had higher (P < 0.05) blood glucose concentrations than the other species, but diet had no effect on this parameter. Blood uric acid concentrations were higher (P < 0.05) after conversion to the commercial diet in P superciliaris. The provision of a commercial extruded diet as a single food source was beneficial, which led to a general improvement in clinical aspects and group uniformity in these 3 species of Cracidae.  相似文献   
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Genetic Resources and Crop Evolution - The biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in...  相似文献   
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Landscape Ecology - Multiscale approaches are essential for understanding ecological processes and detecting the scale of effect. However, nested multiscale approaches retain the effect of the...  相似文献   
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Antibiotic resistance is a global problem, and it is known that commensal bacteria can act as reservoir of antibiotic resistance genes of clinical importance. The aim of the present study was to determine the antibiotic resistance phenotype and mechanisms implicated in resistance of Escherichia coli and Enterococcus spp. isolates collected from fecal samples of 90 Lusitano horses from Portugal. Sixteen of the 71 E. coli isolates (22.5%) recovered showed resistance to at least one of the antibiotics tested. The number of E. coli isolates resistant to streptomycin, tetracycline, chloramphenicol, ampicillin, trimethoprim-sulfamethoxazole, and gentamicin was 9, 7, 6, 3, 2, and 1, respectively. The blaTEM-1 and blaOXA-1 genes were detected in ampicillin-resistant isolates and the sul2 and dfrA1 genes in trimethoprim-sulfamethoxazole-resistant, while the aac(3)-I, floR and tet(A) were found in the gentamicin, chloramphenicol and tetracycline-resistant isolates, respectively. Twenty-two of the 71 (31%) recovered enterococci showed antibiotic resistance for at least one of the tested antibiotics, and resistant isolates were identified as Enterococcus faecium (n = 14), E. faecalis (n = 3), E. hirae (n = 2), and Enterococcus spp. (n = 3). The erm(B) and erm(C) genes were identified in erythromycin-resistant enterococci and the tet(M) and/or tet(L) genes in tetracycline-resistant isolates. The slight prevalence of antibiotic resistance among commensal bacteria of healthy Lusitano horses can improve the treatment of upcoming infections in these horses because these microorganisms can be considered as antimicrobial indicator bacteria.  相似文献   
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OBJECTIVE: To determine M-mode and Doppler echocardiographic reference values in healthy New Zealand white rabbits. ANIMALS: 52 healthy male rabbits. PROCEDURES: The rabbits were anesthetized and M-mode measurements of the left ventricle, left atrium, and aorta and Doppler measurements of pulmonary and aortic outflow and mitral inflow were recorded. RESULTS: Mean +/- SD heart rate during echocardiographic examination was 155 +/- 29 beats/min. Mean +/- SD measurements in diastole and systole for the interventricular septum thickness, left ventricular internal diameter, and left ventricular free wall thickness were 2.03 +/- 0.37 mm and 3.05 +/- 0.45 mm; 14.37 +/- 1.49 mm and 10.25 +/- 1.22 mm; and 2.16 +/- 0.25 and 3.48 +/- 0.55 mm, respectively. Mean +/- SD left atrial-to-aortic diameter ratio was 1.17 +/- 0.14, and mean +/- SD mitral valve E-point-to-septal separation interval was 1.71 +/- 0.29 mm. Mean +/- SD for fractional shortening and ejection fraction were 30.13 +/- 2.98% and 61.29 +/- 4.66%, respectively. Mean +/- SD maximal aortic and pulmonary artery outflow velocities were 0.85 +/- 0.11 m/s and 0.59 +/- 0.10 m/s, respectively, and the peak E-to-peak A wave velocity ratio of the mitral valve was 2.19 +/- 0.46. CONCLUSIONS AND CLINICAL RELEVANCE: Results provide echocardiographic reference values for young adult male New Zealand white rabbits anesthetized with ketamine and medetomidine. Values obtained from unanesthetized rabbits, rabbits sedated with other agents, or rabbits of different size may differ from those reported here.  相似文献   
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The present study investigated the effects of isopulegol, a monoterpene alcohol, in PTZ-induced convulsions and verified possible involved mechanisms. Saline, isopulegol or diazepam were intraperitonealy injected 30 min before PTZ. The latency for development of convulsions and mortality, as well as the mortality protection percentage was recorded. For investigating the involvement of GABAergic system, flumazenil was utilized. The activity of antioxidant enzyme catalase as well as the levels of reduced glutathione and lipid peroxidation were measured in brain hippocampus. Similarly to diazepam, isopulegol significantly prolonged the latency for convulsions and mortality of mice. All animals were protected against mortality at higher dose of isopulegol. Flumazenil pretreatment decreased the prolongation of seizure latency induced by both diazepam and isopulegol, although it was not able to reverse the latency and protection percent for mortality. Isopulegol also significantly prevented PTZ-induced increase in lipid peroxidation, preserved catalase activity in normal levels, and prevented the PTZ-induced loss of GSH in hippocampus of mice. These results suggest that the anticonvulsant and bioprotective effects of isopulegol against PTZ-induced convulsions are possibly related to positive modulation of benzodiazepine-sensitive GABAA receptors and to antioxidant properties.  相似文献   
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