Detection of viruses associated with rugose wood in Japanese grapevines and analysis of genomic variability of Rupestris stem pitting-associated virus   总被引：1，自引：0，他引：1  

Ryoji Nakaune  Koji Inoue  Hideo Nasu  Katsura Kakogawa  Hiromichi Nitta  Jun Imada  Masaaki Nakano 《Journal of General Plant Pathology》2008,74(2):156-163

  相似文献   

A simple, rapid method for gizzerosine analysis in fish meal by paper electrophoresis     

Zhihua Tao  Minoru Sato  Kegang Wu  Hiromasa Kiyota  Toshiyasu Yamaguchi  Toshiki Nakano 《Fisheries Science》2012,78(4):923-926

We established a simple, rapid method for gizzerosine analysis in fish meal. Gizzerosine was extracted from fish meal with 0.1?N HCl solution. Samples and standard gizzerosine solutions were absorbed onto a paper disc, which was then set on electrophoresis paper for 18?min, and the paper was dried. Gizzerosine was visualized with Pauly??s reagent, and the intensity of the colored spots was digitized and calculated by image processing method software. We achieved successful separation of gizzerosine from other Pauly??s reagent-positive components in fish meal extracts. The linearity of gizzerosine estimation using this method was within the range 30?C1000?ng (R ²?=?0.99). Gizzerosine was satisfactorily detected and completely separated from histamine and other Pauly??s reagent-positive compounds. This method does not require expensive instruments or tedious pretreatment to eliminate interfering compounds, such as histamine or histidine. It also uses less reagent compared with high-performance liquid chromatography. Moreover, it is a simple, rapid, sensitive, and reproducible method. It is suitable for monitoring gizzerosine in fish meal products that contain as little as 10?ppm gizzerosine.  相似文献   

Infection of a group of boar-hunting dogs with Paragonimus westermani in Miyazaki Prefecture, Japan     

Kirino Y  Nakano N  Hagio M  Hidaka Y  Nakamura-Uchiyama F  Nawa Y  Horii Y 《Veterinary parasitology》2008,158(4):376-379

A 5-year-old male mixed-bred boar-hunting dog with a Plott hound background weighing 23 kg was brought to the Veterinary Teaching Hospital, University of Miyazaki, in October 2002. The dog was diagnosed with active infection with the lung fluke Paragonimus westermani by serological testing and also by detection of parasite eggs in his feces. Subsequent examination of four other dogs working with this dog as a boar-hunting team revealed that all five dogs were infected with P. westermani.  相似文献   

Quantitative trait loci (QTL) controlling plant architecture traits in a Solanum lycopersicum × S. pimpinellifolium cross     

H. Nakano  K. Sasaki  Y. Mine  K. Takahata  O. Lee  N. Sugiyama 《Euphytica》2016,211(3):353-367

  相似文献   

Meiotic chromosome pairing in intergeneric hybrids of colchicaceous ornamentals revealed by genomic in situ hybridization (GISH)     

Tomonari Kishimoto  Miki Yamakawa  Daisuke Nakazawa  Junji Amano  Sachiko Kuwayama  Masaru Nakano 《Euphytica》2014,200(2):251-257

Diploid and triploid intergeneric hybrids obtained by crosses among Gloriosa superba ‘Lutea’ (2n = 2x = 22), G. ‘Marron Gold’ (2n = 4x = 44), Littonia modesta (2n = 2x = 22), and Sandersonia aurantiaca (2n = 2x = 24) were analyzed for their meiotic chromosome pairing in pollen mother cells by genomic in situ hybridization (GISH) with digoxigenin-labeled total DNA of one parent as probe. Chromosomes from each parent could be clearly distinguished in pollen mother cells of all the five intergeneric hybrids by GISH. For three diploid hybrids, L. modesta × G. superba ‘Lutea’ (2n = 2x = 22), L. modesta × S. aurantiaca (2n = 2x = 23) and S. aurantiaca × G. superba ‘Lutea’ (2n = 2x = 23), 0.04?0.27 autosyndetic bivalents (intragenomic pairing of non-homologous chromosomes) and 0.13?0.36 allosyndetic bivalents (intergenomic chromosome pairing) were observed per pollen mother cell, indicating that there are some homologous chromosomal regions within each genome and among the genomes of Gloriosa, Littonia and Sandersonia. Differences in the average number of allosyndetic bivalents per pollen mother cell among different genome combinations may reflect the evolutionary distances among the three genera, and Gloriosa and Littonia may be closely related to each other, while Sandersonia may have relatively distant relationships with Gloriosa and Littonia. For two triploid hybrids, L. modesta × G. ‘Marron Gold’ (2n = 3x = 33) and S. aurantiaca × G. ‘Marron Gold’ (2n = 3x = 34), no allosyndetic bivalents were observed. Based on the results obtained in the present study, possible utilization of the diploid and triploid intergeneric hybrids for further breeding of colchicaceous ornamentals is discussed.  相似文献   

Comparison of the antioxidant enzymes of broccoli after cold or heat shock treatment at different storage temperatures   总被引：3，自引：0，他引：3  

Zi Zhang  Kohei Nakano  Shigenori Maezawa   《Postharvest Biology and Technology》2009,54(2):101-105

An experiment was conducted to study the association between cold shock treatment (CST) (0 °C, 1 h) and heat shock treatment (HST) (55 °C, 30 s) on antioxidant enzymes of broccoli at different storage temperatures (20, 10, or 5 °C). There was no difference in antioxidant enzyme activities between CST and HST under storage at 20 °C; superoxide dismutase (SOD) and catalase (CAT) activities were increased in comparison with the controls. However, activities of antioxidant enzymes, except for ascorbate peroxidase (APX), with CST were higher than those with HST during storage at 10 °C, while there were opposite results except for CAT activity at 5 °C. These results suggest that CST and HST have the same effect on the antioxidant enzymes of broccoli during 20 °C storage, but have different effects at below 10 °C, which become more pronounced as the temperature is lowered.  相似文献   

Water adsorption properties of bamboo in the longitudinal direction     

Yoshimi Ohmae  Takato Nakano 《Wood Science and Technology》2009,43(5-6):415-422

Differences in the water adsorption properties of moso bamboo (Phyllostachys pubescens) were examined by analyzing the isotherms. Hygroscopicity decreased from the bottom to the top of the culm, and this tendency was marked above about 80% relative humidity. Results of alkali extraction and the analysis of bundle sheath distribution revealed that the distribution of hygroscopic saccharides, for example, hemicelluloses and less-hygroscopic vascular bundles, affects the hygroscopicity, which varies depending on the position of the internode.  相似文献   

Snapshots Analyses for Turbidity Measurements in Water     

Antonio Cesar Godoy  Alberto Yoshihiro Nakano  David Antônio Brum Siepmann  Ricardo Schneider  Felipe Walter Dafico Pfrimer  Oscar Oliveira Santos 《Water, air, and soil pollution》2018,229(12):378

Turbidity has been used as an effective indicator of water quality and it is regulated by national control agencies as a water potability parameter. This work proposes a simple, low-cost, and easily reproducible method based on digitally processed snapshots of electromagnetic radiation beam through a scattering medium in order to measure turbidity. The formazin polymer was used as the standard reference in the experiments to prepare samples from 0.1 to 100 NTU for high turbidity range and from 0.02 to 10 FNU for low turbidity range. The device design is comprised of a webcam and an LED/laser as a light sensor and a radiation source, respectively. The captured scattered light snapshot can be decomposed digitally in color components values and correlated with the turbidity parameter. It is possible to obtain differently device performances changing the light sensor device configuration. The linear regression analyses have shown a distinct relationship among red, green, blue, and grayscale components and the turbidity. At high range, the green component present the best LOD and LOQ values 0.64 and 2.10, respectively, operating with an ordinary webcam and white LED. Nevertheless, the best device performances were obtained with dedicated Raspberry Pi camera modules and white LED for low range turbidity reaching LOD=0.027 and LOQ=0.087 FNU. The figures of merit show recovery between (97.50?101.95%), repeatability (2.11%), within-laboratory reproducibility (2.25%), and limits of quantification (0.087 FNU). The achieved performance device shows the effectiveness of digitally processed snapshots obtained with digital cameras for turbidity measurements.  相似文献   

Marker-Assisted Selection of Xa21 Conferring Resistance to Bacterial Leaf Blight in indica Rice Cultivar LT2     

Hue Thi Nguyen  Quang Hong Vu  Tan Van Mai  Thu Thi Nguyen  Lam Duc Vu  Tung Thanh Nguyen  Long Viet Nguyen  Hien Thu Thi Vu  Hue Thi Nong  Trung Nguyen Dinh  Nakano Toshitsugu  Liet Van Vu 《水稻科学》2018,25(1):52-56

Bacterial leaf blight of rice (BLB), caused by Xanthomonas oryzae pv. oryzae, is one of the most destructive diseases in Asian rice fields. A high-quality rice variety, LT2, was used as the recipient parent. IRBB21, which carries the Xa21 gene, was used as the donor parent. The resistance gene Xa21 was introduced into LT2 by marker-assisted backcrossing. Three Xoo races were used to inoculate the improved lines following the clipping method. Eleven BC₃F₃ lines carrying Xa21 were obtained based on molecular markers and agronomic performance. The 11 lines were then inoculated with the three Xoo races. All the 11 improved lines showed better resistance to BLB than the recipient parent LT2. Based on the level of resistance to BLB and their agronomic performance, five lines (BC₃F₃ 5.1.5.1, BC₃F₃ 5.1.5.12, BC₃F₃ 8.5.6.44, BC₃F₃ 9.5.4.1 and BC₃F₃ 9.5.4.23) were selected as the most promising for commercial release. These improved lines could contribute to rice production in terms of food security.  相似文献   

Greetings from the Editor-in-Chief of ��Paddy and Water Environment��     

Yoshisuke Nakano 《Paddy and Water Environment》2011,9(1):1-1

  相似文献