Cell mediated immunity in equine herpesvirus type 1 infection I. In vitro lymphocyte blastogenesis and serum neutralization antibody in normal parturient and aborting mares.   总被引：1，自引：1，他引：0       下载免费PDF全文

S K Dutta  D L Campbell 《Canadian journal of veterinary research》1977,41(4):404-408

Blastic transformation of peripheral blood mononuclear cells and serum neutralization antibody levels for equine herpesivurs type 1 were measured in 19 mares from three farms at the time of termination of their pregnancy by normal foaling or viral abortion. The stimulation indexes of lymphocytes obtained from the mares from two farms (Farm 1 and 2) which had virus abortions, ranged from 2.1 to 10.8. But there was no significant difference in stimulation index levels between the aborting and normal foaling mares on these two farms. Equine herpesvirus type 1 was isolated from the mononuclear cells of one mare (No. 5) about two months after she aborted. The stimulation index of lymphocytes from that mare was not significantly different from that of other mares on these farms. Stimulation index of lymphocytes from the mares on one farm (Farm 3) where there was no virus abortion or previous history of virus abortion but were exposed to virus antigen from vaccination, ranged from 1.6 to 2.9. The serum neutralization antibody levels were low in most mares ranging from 1/4 to 1/20 and in three mares these were higher. There was no direct correlation between the levels of serum neutralization antibody and stimulation index of lymphocytes from the mares on these farms.  相似文献   

Pathways of bacterial invasion and watermelon seed infection by Acidovorax citrulli          下载免费PDF全文

B. Dutta  Y. Ha  J. T. Lessl  U. Avci  A. C. Sparks  K. L. Johnson  R. R. Walcott 《Plant pathology》2015,64(3):537-544

This study explored the pathways of ingress of Acidovorax citrulli, the causal agent of bacterial fruit blotch of cucurbits, into watermelon seeds. Up until 7 days post‐inoculation (DPI), a significantly higher percentage of watermelon seeds was infected with A. citrulli when the bacteria were applied (c. 1 × 10⁶ colony‐forming units) to stigmas versus ovary pericarps of female flowers. Immunofluorescence microscopy showed that, with stigma inoculation, A. citrulli colonized style and ovary tissues by 1 DPI, and the bacteria co‐localized with pollen germ tubes in these tissues. With ovary pericarp inoculation, A. citrulli cells penetrated the epicarp and mesocarp tissues by 1 DPI but did not reach endocarp until 4 DPI. Finally, manual pollination followed by stigma inoculation led to >53% A. citrulli‐infected seed lots, while A. citrulli was not detected in seeds/ovules generated by stigma inoculation without pollination (chemically induced parthenocarpy). These results show that stigma inoculation results in faster colonization of watermelon ovules by A. citrulli than pericarp inoculation, even though there is no difference in the levels of infection in mature seeds. The data also indicate that pollen germ tubes play an important role in A. citrulli ingress into watermelon seeds via stigmas.  相似文献   

Remote sensing-based assessment of impact of Phailin cyclone on rice in Odisha,India     

Dipanwita Haldar  Rahul Nigam  C. Patnaik  Sujay Dutta  Bimal Bhattacharya 《Paddy and Water Environment》2016,14(4):451-461

Assessing the nature and extent of damage due to natural calamities remains one of the thrust areas in monitoring resource inventory through remote sensing. The effect of the cyclone Phailin and the post-incessant rains during second fortnight of October 2013 on coastal Odisha was studied in terms of rice area flooded, submerged and damaged. Multi-temporal SAR data were analysed to obtain the rice mask, and from this rice mask, the flood affected rice area was determined. Taluka-wise and district-wise crop loss proportion was estimated, and the overall production loss has been estimated. SAR data aided in delineation of flooded regions, while AWiFS NDVI data of subsequent dates showed both continued inundation and crop vigour status post-flood time period. The ground truth indicated that a major portion of the inundated region was not rice but was typha grasses and harvested rice field which should not be accounted as damage to rice crop. The damage on crop yield was difficult to assess; however, the inundation of the crop at panicle initiation and flowering would have impact on grain filling (results in chaffiness) and was considered as completely damaged. Most of the current inundated rice regions fall in this category. It was estimated that a total of 0.167 million hectares and 0.37 million tons of rice crop was lost in the cyclone and floods. The district-level percentage area of rice flooded was communicated to State Remote Sensing Centre in four days timeframe. The overall accuracy obtained for the validation of the ground truth sites was 91.5 %.  相似文献   

Long-term influence of different production systems on potassium buffering capacity of Typic Ustochrept soil     

Debashis Dutta  N. Ravisankar  R. P. Mishra  N. K. Jat  Amit Kumar  P. C. Ghasal 《Journal of plant nutrition》2019,42(13):1472-1482

Imbalanced application of nutrients in the intensively cropped areas results in deterioration of soil fertility. Application of recommended dose of potassium (K) is essential for improving the use efficiency of other nutrients. To assess the buffering capacity of soil, three composite soil samples were collected from the surface soil (0–30?cm) during 2015 from 3 production systems viz., organic, inorganic and integrated which was maintained from 2004 with basmati rice–wheat–Sesbania system under Network Project on Organic Farming at ICAR-Indian Institute of Farming Systems Research, Modipuram. Fractionation of potassium (K) was achieved by sequential extraction of soil samples with distilled water, ammonium acetate and nitric acid in the same order. The relationship between the adsorbed and equilibrium potassium concentration, quantity was determined by plotting Freundlich adsorption isotherms. This was used to determine the buffering capacity and the concentration levels of potassium adsorbed on un-specific sites in the soil. The suitability of the adsorption equation was determined by applying the least square regression analysis. The results revealed that available potassium in the soils ranged from 119.51 to 135.01?mg/kg with 126.02?±?5.24?mg/kg as mean (ammonium acetate method) while water soluble and nitric acid extracted potassium ranged from 28.51 to 29.05?mg/kg and 2594.49?±?19.33?mg/kg (mean) in various production systems. The mean free energy of replacement was found to be ?1998.01?±?28.38?cal/mol indicating that soils have comparatively higher potassium supply under organic system. The potassium buffering capacity of the soils was found to be 0.7462?±?0.16?mg/kg, 0.6295?±?0.20?mg/kg and 0.6774?±?0.09?mg/kg in organic, integrated and inorganic systems, respectively. The amount of potassium adsorbed on un-specific sites of the organic, integrated and inorganic systems was found to be 7.4730?±?1.81, 15.11?±?2.40 and 11.6 89?±?3.58?mg/kg, respectively. It can be concluded that long-term organic production system improves K⁺ buffering capacity of Typic Ustochrept soil as compare to the integrated as well as inorganic production systems.  相似文献   

Rules of benefit distribution: A dynamic impact on Nepalese community forestry organizations     

Bhagwan Dutta Yadav  Hugh Bigsby  Ian MacDonald 《Journal of Sustainable Forestry》2017,36(2):177-197

Participatory approach has been an official part of Community Forestry (CF) since 1989 when Master Plan for the Forestry Sector (1989) was introduced in Nepal. However, many problems related to benefit distribution from CF have emerged because of the way decision-making is influenced by social and institutional structures present at community level, particularly in terms of dominance by wealthy and caste elite to formulate rules. The study used a conceptual approach using elite theory with models that looked at Executive Committee (EC) that used to formulate distribution rules. The study uses Community Forestry User Groups (CFUGs) level data from 31 CFUGs and household data from 310 households in Baglung district, Nepal. The study examined the factors linked with rules of distribution that determined the relative distribution of forest products. The higher the representation of the poor and disadvantaged households on the EC, the greater the benefits to them in terms of greater quantities distributed and longer collection periods. The policy implication of this study is that the forestry organizations help the poor and underprivileged households to build up capacity to undertake leadership roles that influence the formulation of rules through which organizational elite models in favor to them become part of the elite decision-making.  相似文献   

Plasma Concentrations of 13, 14‐Dihydro‐15‐keto‐Prostaglandin F2α, Progesterone and Cortisol During Periparturient Period in Yaks (Poephagus grunniens L.)     

M Sarkar  BC Das  BK Dutta Borah  BS Prakash 《Reproduction in domestic animals》2010,45(3):433-438

An attempt was made to determine plasma concentrations of, 13, 14‐dihydro‐15‐keto‐prostaglandin F_2α (PGFM), cortisol and progesterone during periparturient period in yak. Plasma PGFM level showed an increasing trend beginning day 5 prior to parturition (0.48 ± 0.14 ng/ml) and increased steeply thereafter to reach a peak level (17.16 ± 1.31 ng/ml) on the day of parturition. The levels, then, declined sharply on day 1 postpartum to reach 1.20 ± 0.40 ng/ml and thereafter declined gradually over the days to reach 0.28 ± 0.09 ng/ml on day 20 postpartum and this level was maintained with fluctuation within narrow limits thereafter till conclusion of the blood sampling on day 90 post‐calving. The plasma progesterone concentration on days 7 and 6 before parturition was high (2.10 ± 0.10 and 2.12 ± 0.10 ng/ml, respectively). The level then decreased gradually and abrupt fall was observed 1–2 days before parturition and became basal on day of parturition (0.24 ± 0.04 ng/ml). This basal level was maintained till the end of the blood sampling on day 90 postpartum. Plasma cortisol level showed an increasing trend beginning day 2 prior to parturition (2.36 ± 0.65 ng/ml) and increased steeply thereafter to reach a peak level (26.65 ± 5.28 ng/ml) on the day of parturition. The levels, then, declined gradually over the days and touched 2.36 ± 0.25 ng/ml on day 3 postpartum and this level was maintained with fluctuation within narrow limits thereafter till day 7 post‐calving.  相似文献   

Efficacy of Ovsynch Protocol with Antiprolactin Treatment for Timed Artificial Insemination During Non‐breeding Seasons in Yaks (Poephagus grunniens L.)     

M Sarkar  BK Dutta Borah  BS Prakash 《Reproduction in domestic animals》2010,45(3):393-398

An attempt was undertaken to investigate the efficacy of Ovsynch protocol for timed artificial insemination (TAI) with or without Norprolac (antiprolactin) treatment during non‐breeding season (winter months) in yaks (n = 25). During non‐breeding season, plasma prolactin profile has been reported high due to cold and nutritional stress. The Norprolac dose of i.m. administration was standardized for prolactin suppression. Three different doses viz. 2.5, 5.0 and 7.5 mg were attempted and the dose of 7.5 mg Norprolac i.m. per animal was found to be suitable for suppression of prolactin levels up to 30 h. Ovsynch treatment with Norprolac induced more number of oestrous symptoms per animal (4.8 vs 2.1), higher LH peak concentration (24.01 vs 16.16 ng/ml), longer duration of LH surge (6.8 vs 5.2 h) and higher conception rate (70 vs 30%) in Ovsynch plus Norprolac treated animals compared with animals treated with Ovsynch alone. Therefore, this study clearly indicates the opportunity for practical application of the Ovsynch plus Norprolac protocol for TAI in yaks during non‐breeding seasons.  相似文献   

Isolation and characterization of an enterovirus from baby chicks having an enteric infection II. Physical and chemical characteristics and ultrastructure     

S K Dutta  B S Pomeroy 《Avian diseases》1967,11(1):9-14

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Highly variable ‘Arhar’ simple sequence repeat markers for molecular diversity and phylogenetic studies in pigeonpea [Cajanus cajan (L.) Millisp.]     

Sutapa Dutta  Ajay K. Mahato  Priti Sharma  Ranjeet S. Raje  Tilak R. Sharma  Nagendra K. Singh 《Plant Breeding》2013,132(2):191-196

Pigeonpea is an important tropical grain legume widely cultivated in South and South‐East Asia for versatile end usage as food, feed, fodder and fuel. Recent publication of draft genome sequence of pigeonpea (‘Arhar’) has allowed mining of large numbers of genomic simple sequence repeat (SSR) markers most of which are either not validated or show very little polymorphism in simple agarose gel–based assays. Here, we describe a special category of 370 validated highly variable ‘Arhar’ genomic SSR (HASSR) markers, which provide much higher level of polymorphism than a random set of SSR markers. These markers were validated for consistent amplification in a set of eight pigeonpea varieties and 152 of these (41%) showed allelic polymorphism on agarose gel electrophoresis. Twenty‐four highest polymorphic HASSR markers were used on a larger set of forty pigeonpea varieties and eight wild Cajanus species for the analysis of genetic diversity and phylogenetic relationship. Genomic HASSR markers described here are highly suitable for genetic diversity and phylogenic studies in pigeonpea.  相似文献   

Purification of vitellogenin from the air breathing catfish, Clarias gariepinus     

D. Manohar  G. Damodar Rao  G. Sreenivasulu  B. Senthilkumaran  Aparna Dutta Gupta 《Fish physiology and biochemistry》2005,31(2-3):235-239

Vitellogenin (Vtg) is a female specific glycophospholipoprotein which can be induced both in male and female with estradiol and xeno-estrogens. The basic theme behind the purification of vitellogenin from the fish is to understand the evolutionary relationship and for the purification and characterization of the Vtg receptor. The male catfish, Clarias gariepinus was administrated with estradiol over a period of time for the synthesis of Vtg and the serum was collected. The Vtg was purified from the serum using a two step chromatographic technique. The serum was passed on to DEAE-ion exchange column and the protein was eluted using a salt gradient. The fractions containing the Vtg were pooled and passed onto a gel permeation chromatography column and the pure protein was obtained. The molecular weight is around 200 kDa on the SDS-PAGE and around 520 kDa on the native gel electrophoresis.  相似文献