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21.
Konosuke OTOMARU Yuki NAOI Kei HAGA Tsutomu OMATSU Takehiko UTO Motoya KOIZUMI Tsuneyuki MASUDA Hiroshi YAMASATO Hikaru TAKAI Hiroshi AOKI Shinobu TSUCHIAKA Kaori SANO Sachiko OKAZAKI Yukie KATAYAMA Mami OBA Tetsuya FURUYA Junsuke SHIRAI Kazuhiko KATAYAMA Tetsuya MIZUTANI Makoto NAGAI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2016,78(2):321-324
During surveillance for bovine diarrhea of unknown causes in Japanese black cattle in Kagoshima Prefecture,
Japan, we found two types of novel kobu-like viruses in fecal samples of calves. Sequence analyses revealed
that they had L protein and 2A protein with H-box/NC sequence motif, which are present in kobuviruses.
Phylogenetic analysis revealed that they were related to kobuviruses; however, they clustered apart from other
kobuviruses. In the prevalence study of two types of novel kobu-like viruses, 16.9% and 10.4% prevalence of
these viruses were observed in the feces of diarrheal calves in this area. 相似文献
22.
Naruto FURUYA Teruyuki MASUNAGA Abu Ashraf KHAN Kazuhiro IIYAMA Masaru MATSUMOTO Nobuaki MATSUYAMA 《Journal of General Plant Pathology》2000,66(4):316-322
A new bacterial disease of Russell prairie gentian (Eustoma grandiflorum) was found in Fukuoka Prefecture, Japan, in 1997. This disease was characterized by wilting and yellowing of the foliage.
A cross section of the stem of a diseased plant revealed a tan to yellow-brown discoloration of the vascular tissue. A nonfluorescent,
aerobic, Gram-negative bacterium was consistently isolated from infected plants. The bacteriological characteristics of 10
isolates of the bacterium coincided with those of the reference strains of Burkholderia caryophylli that were isolated from carnations. The bacterium, as well as the reference strains, attacked Russell prairie gentian and
carnation after artificial inoculation and reproduced the symptoms similar to those after natural infections. On the basis
of bacteriological characteristics and pathogenicities, the bacterium was identified as B. caryophylli. This is the first report of a disease caused by B. caryophylli on Russell prairie gentian ; therefore, bacterial wilt of Russell prairie gentian is proposed as the name of the disease.
Received 5 April 2000/ Accepted in revised form 11 July 2000 相似文献
23.
These studies demonstrate that epicuticular wax may effect the water balance of cabbage under high temperature conditions. In ten week old leaves we did not observe that the cristalline structured wax of the variety Kinsyun was reduced as in three week old leaves compared to Sousyu at high day and night temperatures. Neither the wax structure nor the amount of surface wax of eight varieties could be correlated with their ability to build a head. That head formation of the heat tolerant Sousyu stopped at 27/29°C (day/night) suddenly after starting between leaf stage 11 and 15 could not be explained with differences in the optimum of the photosynthesis or water stress.
Growth parameters such as leaf number, length, area and dry weight of additional varieties, Akiou and Braunschweiger, first recommended for summer growing in warm areas and later in a temperate climate, were determined after three month exposure to mean temperature regimes of 27/29, 27/24 and 24/24°C in growth cabins. 相似文献
Growth parameters such as leaf number, length, area and dry weight of additional varieties, Akiou and Braunschweiger, first recommended for summer growing in warm areas and later in a temperate climate, were determined after three month exposure to mean temperature regimes of 27/29, 27/24 and 24/24°C in growth cabins. 相似文献
24.
Sang-Tae?Seo Naruto?FuruyaEmail author Kazuhiro?Iiyama Minoru?Takeshita Yoichi?Takanami Kenichi?Tsuchiya Chun-Keun?Lim 《Journal of General Plant Pathology》2004,70(5):273-277
A total of 88 strains of Erwinia carotovora subsp. carotovora (Ecc) isolated from various host plants in several geographic regions were screened for production of antibacterial substances using the same strains as indicators. Of the 88 strains, 72 produced antibacterial substances. One of these 72 strains, a Brazilian strain Ecc 32, produced an antibacterial substance active against all tested Ecc strains on TSA medium. The antibacterial spectrum of the compound from Ecc 32 strain was limited to closely related strains of soft-rot Erwinia species. Such a narrow spectrum of activity is typical of bacteriocins. The compound produced by Ecc 32 strain, however, was resistant to some enzymes and detergents. Moreover, the compound was heat-stable and active over a wide pH range. The physical characteristics of the compound were not in agreement with those of bacteriocin or carotovoricin. 相似文献
25.
Htet Wai Wai Kyaw Kenichi Tsuchiya Masaru Matsumoto Kazuhiro Iiyama Seint San Aye Myo Zaw Daisuke Kurose Mitsuo Horita Naruto Furuya 《Journal of General Plant Pathology》2017,83(4):216-225
In 2013 and 2014, an extensive survey of bacterial wilt in Myanmar was performed, and 70 strains of Ralstonia solanacearum (Rs) were collected from wilting plants of tomato, potato, chili and eggplant. Myanmar Rs strains were characterized by traditional and molecular methods. Polymerase chain reaction (PCR) test using Rs-specific primer set amplified one specific band (281-bp) from template DNA of all strains. Pathogenicity tests on the four solanaceous plants differentiated the strains into six pathogenic groups. Biovar determination tests showed that biovar 3 strains predominated (63%) among all Rs strains. Biovar 4 strains (7%) were obtained from both tomato and chili strains, whereas biovar 2 (30%) strains were isolated only from potato. Multiplex-PCR analysis indicated that tomato, eggplant and chili strains belonged to phylotype I, whereas potato strains comprised phylotype I and phylotype II. Strains in phylotype I, which was suggested to have originated from Asia, were the most prevalent in all surveyed areas. Phylogenetic analysis based on the endoglucanase (egl) gene sequences revealed that Myanmar strains partitioned into two major clusters that corresponded to phylotype I and II. Strains in phylotype I were further divided into seven subclusters, each corresponding to a distinct sequevar (15, 17, 46, 47, 48, unknown 1 or unknown 2). All strains in phylotype II belonged to sequevar 1. This is the first comprehensive report of the presence of diverse Rs strains in Myanmar. 相似文献
26.
I.B. TONIAL F.B. STEVANATO M. MATSUSHITA N.E. DE SOUZA W.M. FURUYA J.V. VISENTAINER 《Aquaculture Nutrition》2009,15(6):564-568
This study evaluated the omega‐3 (n‐3) fatty acids and the proximate composition of muscle tissue of adult Nile tilapias to select the best feeding time length with a diet containing 70 (g kg?1 wt) flaxseed oil (FO). The results showed that dietary complementation with FO for 45 days is suitable for obtaining high levels of protein (164 g kg?1), total lipids (94 g kg?1), and ash (18 g kg?1). Furthermore, there was a significant difference (P < 0.05) in the reduction of n‐6 and an increase in the concentration of n‐3. With 45 days’ time of FO feeding, fish weight was 532 g and it was improved by the incorporation of total n‐3 (9.8%), consisting of alpha‐linolenic acid (LNA; 6.3%), and n‐3 very long‐chain polyunsaturated fatty acid (n‐3 VLC‐PUFA; 3.5%), and including docosahexaenoic acid (DHA; 1.2%). This gave a better n‐6/n‐3 ratio (1.1) of muscle tissue, a more desirable ratio than the present ratio sometimes as high as 1 : 20 in human diets. The concentrations of n‐3 VLC‐PUFA were higher than those of native Brazilian freshwater fish. Thus, 45 days is the shortest time period required for the inclusion of FO oil in tilapia feed to raise the nutritional value of adult Nile tilapia. 相似文献
27.
Mariko OKAMOTO Hirotaka FURUYA Ikuko SUGIMOTO Masahiro KUSUMOTO Daisuke TAKAMATSU 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(3):390
Paenibacillus larvae and Melissococcus plutonius are the causative agents of American and European foulbroods of honey bees, respectively. Since their virulence and resistance to disinfectants differ depending on the genotypes/phenotypes of the strains, the discrimination of strain types is important for the effective control of these diseases. Methods to detect and differentiate pathogens in honey are useful for surveying the contamination status of beehives/apiaries. In the present study, we selected a sequence (GenBank accession no. ) as the specific target for enterobacterial repetitive intergenic consensus (ERIC) II-type P. larvae strains for the first time and developed a novel multiplex PCR assay that precisely distinguishes between the major types of foulbrood pathogens (ERIC I and II P. larvae and typical and atypical M. plutonius) in one reaction. In addition, we found that commercially available kits designed for DNA extraction from Mycobacterium in feces efficiently extracted DNA from foulbrood pathogens in honey. Using the multiplex PCR assay and DNA extraction kits, all the targeted types of P. larvae and M. plutonius were detected in honey spiked with the pathogens at a concentration of 100 bacterial cells/strain/ml. Moreover, 94% of the Japanese honey samples examined in the present study were contaminated with one or more types of the foulbrood pathogens. These results indicate that the newly developed methods are useful for detecting foulbrood pathogens in honey. The epidemiological information obtained by these methods will contribute to the effective control of foulbroods in apiaries. FI763267相似文献
28.
Saya SHIMADA Makoto NAGAI Hiromitsu MORIYAMA Toshiyuki FUKUHARA Satoshi KOYAMA Tsutomu OMATSU Tetsuya FURUYA Junsuke SHIRAI Tetsuya MIZUTANI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(9):1163-1166
Metagenomic approach using next-generation DNA sequencing has facilitated the detection
of many pathogenic viruses from fecal samples. However, in many cases, majority of the
detected sequences originate from the host genome and bacterial flora in the gut. Here, to
improve efficiency of the detection of double-stranded (ds) RNA viruses from samples, we
evaluated the applicability of S1 nuclease on deep sequencing. Treating total RNA with S1
nuclease resulted in 1.5–28.4- and 10.1–208.9-fold increases in sequence reads of group A
rotavirus in fecal and viral culture samples, respectively. Moreover, increasing coverage
of mapping to reference sequences allowed for sufficient genotyping using analytical
software. These results suggest that library construction using S1 nuclease is useful for
deep sequencing in the detection of dsRNA viruses. 相似文献
29.
Hiroyuki Ura Naruto Furuya Kazuhiro Iiyama Masatomo Hidaka Kenichi Tsuchiya Nobuaki Matsuyama 《Journal of General Plant Pathology》2006,72(2):98-103
Rice plants with bacterial leaf-sheath browning and grain rot were observed in Fukuoka Prefecture in Japan during the autumn
seasons of 1995 and 1996. Burkholderia spp. were consistently isolated from the infected leaf sheaths and grains. These isolates were pathogenic and induced symptoms
of seedling rot, grain rot, and leaf-sheath browning in rice plants, as well as in some orchidaceous plants (cymbidium, dendrobium,
and oncidium leaves), gladiolus leaves, and onion bulbs. On the basis of morphological, physiological and pathological tests,
and species-specific polymerase chain reaction, the isolates were identified as belonging to either Burkholderia glumae or Burkholderia gladioli. B. gladioli, as well as B. glumae, attacked rice plants after artificial inoculation and reproduced the symptoms similar to those after natural infections.
We confirmed that rice is an additional natural host of B. gladioli. It is clarified that bacterial grain rot of rice is caused not only by B. glumae but also by B. gladioli. 相似文献
30.
Daisuke Kurose Long H. Hoang Naruto Furuya Minoru Takeshita Toyozo Sato Seiya Tsushima Kenichi Tsuchiya 《Journal of General Plant Pathology》2014,80(2):147-152
A preemergence damping-off of tobacco (Nicotiana tabacum L.) was found in Fukuoka, Japan, in 2009. A fungus repeatedly isolated from the black-rotted seeds reproduced the symptoms in tobacco seeds, and the preemergence damping-off developed after flower and soil inoculation with the fungus. Brown spots and blight developed on leaves of tobacco seedlings after inoculation with the isolate. The fungus was reisolated from the rotted seeds and blighted leaves. The causal fungus was identified as Stemphylium lycopersici based on morphology and phylogenetic analysis. This is the first report to confirm the pathogenicity of S. lycopersici in tobacco in the world. 相似文献