Color and surface chemistry changes of extracted wood flour after heating at 120 °C     

Yao Chen  Mandla A. Tshabalala  Jianmin Gao  Nicole M. Stark  Yongming Fan 《Wood Science and Technology》2014,48(1):137-150

To investigate the effect of heat on color and surface chemistry of wood flour (WF), unextracted, extracted and delignified samples of commercial WF were heated at 120 °C for 24 h and analyzed by colorimetry, diffuse reflectance visible (DRV), attenuated total reflectance Fourier transform infrared (ATR-FTIR) and Fourier transform Raman (FT-Raman) spectroscopies. Unextracted samples showed a slight increase in CIEL^*a^*b^* color coordinates, a ^* and b ^*. Compared with unextracted samples, color changes of the extracted samples varied with composition of the extraction solvents with generally smaller increases in a ^* and larger decreases in b ^* values. Delignified samples were marked by even larger increases in both a ^* and b ^* values. The color changes could be explained by analysis of the respective DRV, FTIR and FT-Raman spectra of the samples before and after heating. Heating of the extracted WF at 120 °C resulted in a red shift of the absorption at 430 nm and increase in absorption in the violet-blue spectral region of visible light. Delignified samples showed even more pronounced absorption in this spectral region after heating.  相似文献   

Biofilm development within a larval rearing tank of the tropical rock lobster, Panulirus ornatus     

David G. Bourne  Lone Hj  Nicole S. Webster  Jennie Swan  Michael R. Hall 《Aquaculture (Amsterdam, Netherlands)》2006,260(1-4):27-38

The role of bacterial biofilms in disease processes is becoming increasingly recognised in both clinical and environmental settings. Biofilm development within a rearing tank of the tropical rock lobster Panulirus ornatus was studied to evaluate if the biofilm is a reservoir for potentially pathogenic bacteria that cause mass larval mortalities. Within a 5000 L larval rearing tank, fiberglass microscope slides were systematically distributed during a standard rearing attempt to assess biofilm development. Culture-based counts for two media types, TCBS and Marine Agar (MA), demonstrated increased bacterial densities until days 11 and 13 respectively. For both media types, a drop in the plate counts was followed by a subsequent increase towards the end of the experiment. Scanning electron microscopy (SEM) confirmed that cell densities decreased between days 13 and 17, most likely due to sloughing of the biofilm into the water column. SEM images revealed distinct changes in dominant morphologies reflecting a succession of bacterial populations. A dynamic succession of microbial species during biofilm development was also demonstrated using denaturing gradient gel electrophoresis (DGGE) profiling of bacterial 16S rRNA genes in combination with statistical ordination analysis. Prominent changes in the DGGE profiles coincided with the decrease in bacterial numbers observed by SEM and plating on MA between days 13 and 17. Fluorescence in situ hybridization (FISH) identified -Proteobacteria as being numerically abundant in the biofilm. This was supported by results from DGGE analysis, which retrieved only sequences affiliated with - and γ-Proteobacteria. DGGE bands affiliated with Vibrio became dominant towards the end of the larval run (days 21 to 24). A Vibrio harveyi strain isolated from the biofilm late in the larval rearing trial (day 24) demonstrated increased larval mortality in small scale phyllosoma survival studies. The detection of Vibrionaceae at the end of the larval trial coincided with mass phyllosoma mortality and show that the biofilm is a reservoir for potentially pathogenic bacteria.  相似文献   

Fillet shelf-life of Atlantic halibut Hippoglossus hippoglossus L. fed elevated levels of α-tocopheryl acetate     

Nicole Ruff  Richard D FitzGerald  Thomas F Cross  & Joe P Kerry 《Aquaculture Research》2002,33(13):1059-1071

Fish fillet quality may be influenced by the antioxidant level in preslaughter diet. Thus, the effects of dietary α‐tocopheryl acetate supplementation and feeding time on the flesh quality of farmed Atlantic halibut Hippoglossus hippoglossus L. were investigated. Halibut of mean initial weight of 312 ± 12.3 g were divided into two groups and fed commercial diets, supplemented with different levels of α‐tocopheryl acetate at the dietary inclusion levels of 189 and 613 mg kg^?1 diet. Fish were sampled after 6,9,12 and 24 weeks. Over the experimental period, they reached a final mean weight of 1320 ± 108.4 g. Tissue α‐tocopherol of fillet and liver was significantly affected by the levels of α‐tocopheryl acetate given with the diets (P < 0.001). In storage on ice, fillets of fish fed the diets high in α‐tocopheryl acetate exhibited significantly lower (P < 0.001) levels of lipid oxidation. The colour of fillets in all groups deteriorated slightly, but diet did not affect this process. Halibut fed the supplemented diets for longer periods were better protected against lipid oxidation (P < 0.001) and colour deterioration (P < 0.01) than those fed for shorter periods. However, after 9 days of storage, lipid oxidation levels were still extremely low [< 0.6 µg malondialdehyde (MDA) g^?1 fillet], even in fillets of fish fed the low α‐tocopheryl acetate diet for a short period preslaughter. Different slaughtering methods tested at the end of the trial showed that percussive stunning can delay the onset of rigor mortis by 8–12 h compared with bleeding of the fish. These results suggest that halibut fillets have enhanced shelf‐life stability even at low doses of dietary α‐tocopheryl acetate, and that other factors in the antioxidant defence mechanisms of the species might play a major role in the prevention of lipid oxidation.  相似文献   

Livestock and poultry fitness for transport — The veterinarian’s role     

Gord Doonan  Geneviève Benard  Nicole Cormier 《The Canadian veterinary journal. La revue veterinaire canadienne》2014,55(6):589-590

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Use of Best Management Practices and Pasture and Soil Quality on Maryland Horse Farms     

Nicole M. Fiorellino  MS  Joshua M. McGrath  Bahram Momen  Solomon K. Kariuki  Michael J. CalkinsAmy O. Burk  PhD 《Journal of Equine Veterinary Science》2014

Agricultural operations, including horse farms, can contribute nonpoint source (NPS) pollution to surface water. The use of best management practices (BMPs) is the most effective way to prevent the movement of pollutants to surface water from nonpoint source pollution. Previous mailed survey studies have assessed the use of BMPs at the county and state level, but a visual assessment of horse farms is necessary to validate survey results. An observational field study was conducted to assess BMP use and soil and pasture quality and to create a model to predict soil erosion on Maryland horse farms. Fifty-one farms were selected based on stocking density (acres per horse [ac horse^-1]), farm use, and presence of water on property. All farms were visited from September through November 2009. In each pasture with grazing horses, the correct use of BMPs was assessed, grass height and vegetative cover were measured, and composite soil samples were collected. Less than half of the 18 assessed BMPs were being used by participants. Although most participants maintained the recommended vegetative cover and grass height, soil erosion was a major problem in pastures. Most farms had optimum soil nutrient concentrations (Ca, K, and P), excessive Mg values, and basic soil pH. Vegetative cover and grass height measurements were positively correlated with stocking density (r = 0.345, P < .0001; and r = 0.291, P < .0001, respectively). Farm use was the only variable that predicted soil erosion on farms (P = .006). Farms used for pleasure were least likely to have soil erosion, whereas farms used for breeding were more likely to have soil erosion (P = .0058). Despite the low-to-moderate adoption of BMPs, the maintenance of recommended vegetative cover and grass height as well as optimum values of soil nutrients indicated participating Maryland horse farms have a low potential for nutrient movement and NPS pollution.  相似文献   

Ostreid herpesvirus type 1 replication and host response in adult Pacific oysters,Crassostrea gigas     

Amélie Segarra  Laury Baillon  Delphine Tourbiez  Abdellah Benabdelmouna  Nicole Faury  Nathalie Bourgougnon  Tristan Renault 《Veterinary research》2014,45(1)

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Evaluation of intraorbital prosthetic pigmentation using 0.3 and 1.5 Tesla magnetic resonance imaging and computed tomography     

D. Dustin Dees  Nicole E. MacLaren  Kevin J. Fritz  Michael R. Broome  Douglas W. Esson 《Veterinary ophthalmology》2014,17(3):184-189

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Resistance testing and the effectiveness of difenacoum against Norway rats (Rattus norvegicus) in a tyrosine139cysteine focus of anticoagulant resistance,Westphalia, Germany     

Alan Buckle  Stefan Endepols  Nicole Klemann  Jens Jacob 《Pest management science》2013,69(2):233-239

BACKGROUND: Anticoagulant resistance in Norway rats at foci in Belgium, Denmark, France, Germany, the Netherlands and the United Kingdom is genetically characterised by the same single nucleotide polymorphism (SNP) and consequent amino acid exchange from tyrosine to cysteine at location 139 of the vkorc1 gene (i.e. tyrosine139cysteine or Y139C). The purpose of this study was to assess the degree of resistance among rats at two infested farm sites in the Y139C focus in Westphalia, Germany, using blood clotting response (BCR) tests, and to determine the practical efficacy of applications of a commercial 50 ppm difenacoum bait (Neokil^?) against them. RESULTS: BCR tests showed that the difenacoum resistance factor (RF) among the Y139C rats was about 2.5. DNA analysis for the Y139C mutation revealed that it was present among rats at the two sites with a prevalence of 75 and 93%. Applications of difenacoum bait at the two sites achieved 86.8 and 59.9% control. The different outcomes did not appear to be due to differences either in the degree and prevalence of resistance or in the quantities of poisoned bait consumed. CONCLUSION: The study showed that, although the RF for difenacoum among rats carrying the Y139C SNP was apparently low, an acceptable level of control of resistant Norway rat infestations was not achieved using difenacoum. Continued use of anticoagulants against rats that are resistant to them will exacerbate resistance problems in terms of both increased severity and prevalence. These conclusions are likely to apply elsewhere in Europe where the Y139C SNP occurs. Copyright © 2012 Society of Chemical Industry  相似文献   

New sources of resistance to Meloidogyne incognita race 3 in wild cotton accessions and histological characterization of the defence mechanisms     

F. C. Mota  G. C. S. Alves  M. Giband  A. C. M. M. Gomes  F. R. Sousa  V. S. Mattos  V. H. S. Barbosa  P. A. V. Barroso  M. Nicole  J. R. Peixoto  M. R. Rocha  R. M. D. G. Carneiro 《Plant pathology》2013,62(5):1173-1183

Accessions of Gossypium spp., some of them never previously tested, were evaluated for resistance to a local isolate of Meloidogyne incognita race 3 in greenhouse experiments. Nematode infection was characterized based on the galling and egg mass indexes and the reproduction factors (RF). Root‐knot nematode reproduction among the newly tested accessions varied from highly susceptible (AS0188, AS0189) to moderately resistant (MT123 no. 3), and some accessions showed highly reduced nematode reproduction (CIR1343, CIR1348, Fai Mui). Histological observations of two resistant accessions (G. barbadense CIR1348 and G. hirsutum TX‐25, respectively) showed that resistance occurs through a two‐stage mechanism in the first accession and through a single‐stage mechanism in the second. Parasitism is blocked early after second‐stage juvenile (J2) penetration or during its initial tissue migration (CIR1348) and the development of later‐stage juveniles into female adults is suppressed at a later stage (TX‐25 and CIR1348). Fluorescence and bright light microscopy showed that root cells surrounding nematodes exhibit a hypersensitivity‐like reaction, with the accumulation of presumably phenolic compounds and the presence of necrotic cells that limit the development of nematodes and the formation of giant cells. Underdeveloped giant cells with degenerated cytoplasmic content were found in small numbers in CIR1348 and in large numbers in TX‐25, along with deformed nematodes. The full characterization of the defence mechanisms of novel sources of resistance to the root‐knot nematode in cotton constitutes a first step towards their use in crop improvement.  相似文献   

Development of two species-specific primer sets to detect the cereal cyst nematodes Heterodera avenae and Heterodera filipjevi   总被引：1，自引：0，他引：1  

Fateh Toumi  Lieven Waeyenberge  Nicole Viaene  Amer Dababat  Julie M. Nicol  Francis Ogbonnaya  Maurice Moens 《European journal of plant pathology / European Foundation for Plant Pathology》2013,136(3):613-624

Twelve Heterodera species are of major economic significance in wheat and barley. Of these, H. avenae, H. filipjevi and H. latipons are among the most important ones, and sometimes coexist. The identification of Heterodera species using morphological characteristics is time consuming, requires specialized skill and can be imprecise, especially when they occur mixed in field populations. Molecular techniques can provide a more accurate way for nematode identification. This study reports the results of experiments targeting the mitochondrial cytochrome oxidase subunit 1 (COI) gene to develop species-specific primers that could be used for the identification of H. avenae and H. filipjevi. The COI gene of 9 Heterodera spp. and Punctodera punctata was partially sequenced and the resultant sequences were aligned to find unique sites suitable for the design of primers. The alignment showed variability between H. avenae, H. filipjevi and other Heterodera species. Two sets of species-specific primers were identified for the identification of both species and the conditions for their use in PCR were optimised. The specificity of the designed primers was checked by comparison with one population of P. punctata and populations of 14 other Heterodera species, nine populations of H. avenae and 10 populations of H. filipjevi originating from different countries. To test the sensitivity, the PCR was run with DNA extracted from five second-stage juveniles (J2) of H. avenae or five J2 of H. filipjevi mixed with DNA extracted from varying numbers of J2 of H. latipons. It was possible to detect as few as five J2 of H. avenae or H. filipjevi among 100 J2 of H. latipons. The two primers sets allow the detection of H. avenae and H. filipjevi where they occur in mixed populations with other Heterodera spp.  相似文献