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11.
12.
Sea urchin, Strongylocentrotus intermedius, transplanted from Japan in 1989, has been widely cultured along the coasts of Liaoning and Shandong Provinces and has become the dominant and most economically important maricultured species in North China. However, a lesion syndrome symptom of S. intermedius broke out frequently these years, showing lethargy in activities, blackish peristomial membrane and body well lesions, and brought about high mortality eventually. Six representative prominent bacterial strains were isolated from diseased sea urchin from September 2009 to January 2010. By means of API 20NE and 16S rRNA gene sequences analysis, isolates were identified as Shewanella aquimarina, Pseudoalteromonas tetraodonis, Vibrio shilonii, V. harveyi, V. fortis and V. splendidus. Bacterial challenge tests showed that their representative isolates were virulent to S. intermedius with LD50 values ranging from 9.2 × 104 to 3.4 × 106 CFU/g body weight, among which S. aquimarina , V. fortis and P. tetraodonis were highly virulent, and the other three isolates showed moderate virulence. The results indicated that a variety of bacteria including Shewanella, Pseudoalteromonas and Vibrio were involved in the mortality of S. intermedius, and the six isolates were opportunistic pathogens of sea urchins. All isolates reported herein were sensitive to ampicillin, enrofloxacin, ofloxacin, doxycycline and florfenicol.  相似文献   
13.
LINTUL3 is a crop model that calculates biomass production based on intercepted photosynthetically active radiation (PAR) and light use efficiency (LUE). It is an adapted version of LINTUL2 (that simulates potential and water-limited crop growth), including nitrogen limitation. Nitrogen stress in the model is defined through the nitrogen nutrition index (NNI): the ratio of actual nitrogen concentration and critical nitrogen concentration in the plant. The effect of nitrogen stress on crop growth is tested in the model either through a reduction in LUE or leaf area (LA) or a combination of these two and further evaluated with independent datasets. However, water limitation is not considered in the present study as the crop is paddy rice. This paper describes the model for the case of rice, test the hypotheses of N stress on crop growth and details of model calibration and testing using independent data sets of nitrogen treatments (with fertilizer rates of 0–400 kg N ha?1) under varying environmental conditions in Asia. Results of calibration and testing are compared graphically, through Root Mean Square Deviation (RMSD), and by Average Absolute Deviation (AAD). Overall average absolute deviation values for calibration and testing of total aboveground biomass show less than 26% mean deviation from the observations though the values for individual experiments show a higher deviation up to 41%. In general, the model responded well to nitrogen stress in all the treatments without fertilizer application as observed, but between fertilized treatments the response was varying.  相似文献   
14.
Biology, damage pattern, incidence levels and status of the leaf miner Aphanisticus aeneus Kerremans (Coleoptera: Buprestidae) were studied in accessions of Saccharum spp. Egg, grub and pupal periods of the miner lasted 6.0 ± 0.8, 18.5 ± 1.9 and 7.0 ± 1.3 days, respectively; adults lived for 8–10 days. The grub tunneled the leaf on the lower surface just above the epidermis forming a linear channel; infested tissues showed brownish discoloration. The extent of leaf miner attack among the 331 accessions of Saccharum spp. ranged 4.00%–76.47% on cane basis and 7.14%–100.00% on leaf basis; the mined leaf area ranged 3.22–11.00 cm2. When the accessions were grouped on the basis of an infestation index developed with the above three parameters, 267 occupied very low category, 51 were in low category, 11 were in moderate category and 2 were placed in high incidence category. All accessions showed miner attack in at least one study year. Within each of the four Saccharum spp., accessions differed significantly among the three study years for all infestation parameters. Among the four species, while S. robustum accessions recorded significantly highest infestation index, the other three species remained on par.  相似文献   
15.
A field experiment was conducted to evaluate the effect of integrated use of farmyard manure and bio-inoculants on wheat productivity for two years in succession. Increasing levels of farmyard manure (FYM) up to 15 t ha?1 significantly (p ≤ 0.05) improved the dry matter accumulation, effective tillers per m row length, and grain weight per spike in both the years. Application of 15 t ha?1 FYM caused significant increase in spikelets per spike and grains per spike over control and 5 t ha?1 during two consecutive years. Inoculation with MSX-9 strain of Azotobacter chroococcum produced significantly higher dry matter accumulation to 25.63, 13.33, 7.78 and 23.66, 8.35, 5.50% over uninoculation, Azospirillum brasilense (SP-7) and Azospirillum lipoferum (A-5) at harvest during 1999–2000 and 2000–2001, respectively. Incorporation of 15 t ha?1 FYM significantly (p ≤ 0.05) enhanced grain and straw yield to 62.45 and 38.05%; 56.66 and 36.28%; 59.42 and 37.52% over control in 1999–2000, 2000–2001 and pooled analysis, respectively. The grain and straw yield of wheat significantly (p ≤ 0.05) enhanced to 26.51, 10.10, 7.54 and 14.45, 5.77, 3.16% through A. chroococcum (MSX-9), A. brasilense (SP-7) and A. lipoferum (A-5) over uninoculation.  相似文献   
16.
Simulation modeling of perennial crops has immense potential for generating information for plantation managers. We report the development of the InfoCrop-coconut model and its application to coconut (Cocos nucifera L.) growing in diverse tropical and subtropical environments. The model is based on the generic crop model InfoCrop that simulates various annual crops in tropical and subtropical regions. The InfoCrop-coconut model was calibrated and validated with data compiled from published studies comprising many physiological, agronomical and nutritional experiments conducted between 1978 and 2005 in diverse geographic locations throughout India. The treatments included various water and nutrient regimes and varieties of coconut. Time to first flowering varied between 4 and 6 years, leaf production varied from 8 to 15 leaves year(-1) and nut yield ranged from 3000 to 27,000 nuts ha(-1) year(-1). The genetic coefficients used for calibration and validation were generated from field experiments conducted during 1995-2005. Model efficiency and validation performance were analyzed statistically. Simulated trends in phenological development, total dry mass and its partitioning, and nut yield agreed closely with observed values, although a 15% error was observed in a few cases. Considering that field measurements have an experimental error of 10-15% and wide variation existed within treatments, the model adequately simulated the effects of management practices and agro-climatic conditions over short periods. For a range of agro-climatic zones, simulated potential yields varied from 26 to 30 Mg ha(-1) year(-1) and potential annual dry mass production varied from 52 to 62 Mg ha(-1), depending on environment. We conclude that InfoCrop-coconut can be used to increase the efficiency of agronomic experiments designed to aid coconut crop management.  相似文献   
17.
Equine luteinizing hormone (eLH), equine follicle stimulating hormone (eFSH) and equne chorionic gonadotropin (eCG) were desialylated and their plasma disappearance, tissue uptake and degradation determined. Both native hormones and their desialylated derivatives were radioiodinated and injected intravenously into male rats. Plasma samples were taken at different time intervals and examined for both total and trichloroacetic acid (TCA) precipitable radioactivity. Disappearance curves for each hormone consisted of two exponential components: a fast clearance followed by a slow phase. The apparent t12 of the fast component for native eCG, eLH and eFSH were 18.3 min, 17.0 min and 17.1 min, respectively; this is in contrast to their desialylated derivatives which had a t12 of 2.8 min, 4.9 min and 7.1 min, respectively. The slower component for native and desialylated eCG had at t12 of 355.5 min and 171.2 respectively. After TCA precipitation more than 90 percent of the radioactivity in plasma was in the pellet, whereas in urine almost 95 percent of the radioactivity was in the TCA supernatant. Coincident with the disappearance of the hormone from the plasma its uptake and release was observed in liver, kidney, testis, and muscle tissue. Gel filtration data on plasma and urine samples indicated that the former had mostly intact hormone in contrast with the latter. Together these results indicate that eLH, eFSH and eCG disappear from the plasma of male rats in a biphasic manner with a rate slower than their desialylated derivatives.  相似文献   
18.
The present study was undertaken to estimate effect of various genetic and non-genetic factors on persistency of milk production and to identify the most appropriate persistency method that fits best in our environment. In the present study, effects of different non-genetic factors, viz. year, season, days to attain peak yield, and genetic group based on the level of exotic inheritance on persistency of milk yield in crossbred cattle were studied. Data comprised of 686 first lactation daily milk yield records of crossbred cattle that were maintained at GADVASU dairy farm over a period of 25 years from 1991 to 2015 were utilized to calculate persistency coefficients by four methods, viz., Ludwick and Peterson method (P1), Mahadevan method (P2), ratio method (P3), and Prasad et al. method (P4). Overall least squares means for persistency by Ludwick and Peterson method (P1), Mahadevan method (P2), ratio method (P3), and Prasad et al. method (P4) were 0.896?±?0.096, 1.385?±?0.224, 187.207?±?26.398, and 0.621?±?0.098, respectively. Effect of sires was significant (P?<?0.05) on P2 and P4 methods. Effect of genetic group on all four methods was non-significant. Period of calving had significant (P?<?0.01) effect on persistency of milk yield (P2, P3, and P4 methods). Effect of season of calving on persistency of milk yield was found to be significant in all estimates obtained by the four methods. Summer and autumn calvers were most persistent whereas spring and winter calvers were least persistent for (P2, P3, and P4 methods). Persistency of milk yield was significantly (P?<?0.05) affected by days to attain peak yield in P1 and P2 methods. Maximum persistency was obtained in animals attaining peak at 41–57 days of lactation and minimum in <?41 days for Mahadevan method and ratio method. The highest heritability of persistency and minimum value of standard error was estimated as 0.275?±?0.11 for the Mahadevan method followed by the Prasad method (0.197?±?0.10) by half sib correlation method. The maximum coefficient of variation which indicates available variability was estimated as 20.788% for persistency by the Mahadevan method followed by 18.969% for the Prasad method. The highest correlation was also observed between P1 and P3 methods by Spearman’s and Pearson’s correlation for least squares breeding value of the sires. On the basis of heritability, standard error of heritability, and coefficient of variation, it can be concluded that the Mahadevan method followed by the Prasad method suits best to our environment for animals in first lactation as well as they can be utilized for effective selection for higher persistency in crossbred animals of Punjab.  相似文献   
19.
The effects of transglutaminase (TG) on the properties of semolina dough and pasta cooking properties in durum‐only and fiber‐enriched pasta were investigated. TG was blended at levels 0, 0.05, 0.1, 0.25, 0.5, and 1% of semolina weight with semolina and semolina‐pollard (60% w/w) and semolina‐guar gum (15%) mixtures. The addition of TG increased dough maximal resistance, making the dough inextensible at >1%. Optimum effects on dough strength were obtained at 0.5% TG; this dough gave the firmest and least sticky pasta. A more extensive and thicker protein matrix was observed in the TG pasta by confocal scanning laser microscopy, indicating more cross‐links were formed, a finding supported by measuring percentage of unextracted polymeric protein. TG was unable to overcome the negative effect of 60% pollard on cooking loss or 15% guar gum on stickiness. Gluten was generally more effective than TG in restoring the properties of pastas with added fiber.  相似文献   
20.
In laboratory incubations, the time to 50% loss of napropamide was approximately 60; 21 and 8 days in soil treated for the first, second and third time respectively. In a survey of soils from commercial fields, there was evidence that enhanced biodegradation of the compound had been induced by normal field applications—in some soils by a single previous treatment. Confirmation of the observations of rapid rates of loss in pre-treated soil was obtained in experiments with three formulations of napropamide. The rate of degradation in enhanced soils was unaffected by treatment of the soils with the antifungal antibiotic cycloheximide, but was inhibited by the antibacterial antibiotic chloramphenicol. Mixed bacterial cultures able to degrade the herbicide were obtained from three rapid-degrading soils by enrichment culture. Isolates from two of them were able to degrade the herbicide in pure culture. These bacteria have, as yet, not been characterised.  相似文献   
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