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11.
RAMJI KUMAR BHANDARI IKUO USHIKOSHI HIDEO FUKUOKA NOBUHISA KOIDE KOHEI YAMAUCHI HIROSHI UEDA 《Fisheries Science》2002,68(4):776-782
ABSTRACT: The filamentous fungi Rhizopus , like many fungal species, possesses physiologically active substances. Rhizopus extract (RU) is reported to be effective for various aspects of growth and reproduction in many vertebrates. The effects of RU administration on body growth and plasma levels of steroid hormones were investigated in lacustrine sockeye salmon Oncorhynchus nerka . One-year-old fish were fed daily with RU (20 mg/kg feed) from July 1999 to October 2000 for 15 months. Fish were sampled every month and plasma levels of testosterone (T), 11-ketotestosterone (11-KT), estradiol-17β (E2 ) and 17α,20β-dihydroxy-4-pregnen-3-one (DHP) were measured by time-resolved fluoroimmunoassay. Body growth of RU-fed fish of both sexes increased significantly in 1+ and 2+ October, and 2+ January–March and July. All RU-fed males and one female matured in 2+ October. RU-fed 1+ precociously mature males showed increased plasma levels of T, 11-KT and DHP in 1+ October. In 2+ males, RU significantly elevated plasma levels of T from May to June, 11-KT from June to July, and DHP in October. In sockeye salmon, administration of RU accelerated body growth of both sexes and sexual maturation in males, suggesting physiologically active substances present in RU enhance somatic growth and sexual maturation by sex-specific mechanisms. 相似文献
12.
Takeshi TSUKA Naoki YAMAMOTO Makoto SANESHIGE Takehito MORITA Yuji SUNDEN Yusuke MURAHATA Kazuo AZUMA Tomohiro OSAKI Norihito ITO Yoshiharu OKAMOTO Tomohiro IMAGAWA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(12):1689-1691
A 2-month-old male Japanese Black calf was presented with a 30-day history of progressive
ataxia. Antemortem examination using computed tomography (CT) revealed narrowing of the
disc spaces due to destruction of intervertebral structures between the first and second
thoracic vertebrae and between the second and third thoracic vertebrae. Osteolysis was
evident as irregular hypoattenuating lesions within the opposing end plates of the first,
second and third thoracic vertebrae. Pseudomonas aeruginosa was detected as the causative
bacteria, and discospondylitis was diagnosed. To the best of our knowledge, this is the
first bovine case report describing the application of CT for the diagnosis of
discospondylitis. 相似文献
13.
Takuya HIROSE Takayuki OGURA Keisuke TANAKA Jun MINAGUCHI Takeshi YAMAUCHI Toshiyuki FUKADA Yoh-ichi KOYAMA Kazushige TAKEHANA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1385-1389
Ehlers-Danlos syndrome (EDS) is a group of disorders caused by abnormalities that are
identified in the extracellular matrix. Transforming growth factor-β1 (TGF-β1) plays a
crucial role in formation of the extracellular matrix. It has been reported that the loss
of function of zinc transporter ZRT/IRT-like protein 13 (ZIP13) causes the spondylocheiro
dysplastic form of EDS (SCD-EDS: OMIM 612350), in which dysregulation of the TGF-β1
signaling pathway is observed, although the relationship between the dermis abnormalities
and peripheral TGF-β1 level has been unclear. We investigated the characteristics of the
dermis of the Zip13-knockout (KO) mouse, an animal model for SCD-EDS.
Both the ratio of dermatan sulfate (DS) in glycosaminoglycan (GAG) components and the
amount of collagen were decreased, and there were very few collagen fibrils with diameters
of more than 150 nm in Zip13-KO mice dermis. We also found that the
TGF-β1 level was significantly higher in Zip13-KO mice serum. These
results suggest that collagen synthesis and collagen fibril fusion might be impaired in
Zip13-KO mice and that the possible decrease of decorin level by
reduction of the DS ratio probably caused an increase of free TGF-β1 in
Zip13-KO mice. In conclusion, skin fragility due to defective
ZIP13 protein may be attributable to impaired extracellular matrix
synthesis accompanied by abnormal peripheral TGF-β homeostasis. 相似文献
14.
Masaharu Kubota Osamu Tamura Yasuhiro Nomura Noriko Orihara Norihito Yamauchi Kengo Yonemoto Kenichiro Chiba Nobutaka Someya 《Journal of General Plant Pathology》2017,83(2):117-120
Isolates of spinach downy mildew fungus, Peronospora farinosa f. sp. spinaciae, collected at seven sites in Japan from December 2013 to March 2016, were identified as belonging to races 8, 10, 12 and 13, and one was unauthorized. Races 10, 12, 13 and the unauthorized were first found in Japan. 相似文献
15.
NAOTAKA OMOTO MAMORU MAEBAYASHI ERI MITSUHASHI KOJI YOSHITOMI SHINJI ADACHI KOHEI YAMAUCHI 《Fisheries Science》2002,68(5):1047-1054
The effects of giving oral estradiol-17β (E2 ) and 17α-methyltestosterone (MT) on gonadal sex differentiation in the F2 hybrid sturgeon, the bester ( Huso huso female × Acipenser ruthenus male), are investigated. Giving E2 at 10 μg/g diet to fish from 14 months until 31 months of age induced incomplete feminization and resulted in approximately 40% abnormal ovary development in which oocytes were observed without ovarian lamellar structures and gonadal shape was similar to normal testis. Giving MT at 25 μg/g diet for the same duration failed to induce masculinization, and resulted in approximately 30% undeveloped gonads even at 30–37 months of age. In contrast, E2 and MT at only 1 μg/g diet given from 3 to 18 months of age was sufficient to induce feminization and masculinization, respectively. In these fish, feminization and masculinization were observed at 9 months, when most putative ovaries and testes were histologically distinguishable by the shape of the gonadal surface. These results indicate that sex reversal can be induced in these fish by hormone treatment that is started at 3 months age, before morphological differentiation occurs on the stroma of the gonads. 相似文献
16.
Fukui Y Togawa M Abe N Takano Y Asada M Okada A Iida K Ishikawa H Ohsumi S 《The Journal of reproduction and development》2004,50(1):147-154
The object of the present study was to investigate the validation of the sperm quality analyzer (SQA) and the hypo-osmotic swelling (HOS) test with standard sperm analysis methods in frozen-thawed ram and minke whale spermatozoa. In rams, highly significant correlations were observed in the percentage of motile spermatozoa (P<0.01) and sperm concentration (P<0.01) between the standard and SQA methods. But, the percentage of morphologically normal spermatozoa did not significantly correlate between the standard and SQA methods. The percentages of swollen spermatozoa at 15 minutes by the HOS test were significantly correlated with the motility by the standard (P<0.05) and by the SQA (P<0.05) methods. For minke whale spermatozoa, the SVI (sperm viability index) values by the standard method were significantly (P<0.001) correlated with the sperm motility index (SMI) values by SQA. The percentage of motile spermatozoa was also significantly correlated (P<0.01) with the motility measured by SQA. Using different hypo-osmotic solutions and incubation times, the HOS test with 25, 100 and 150 mOsM did not show significant variations. Motility observed by the standard method and the percentage of swollen spermatozoa were significantly correlated (P<0.05). These results indicate that the SQA and HOS test can be utilized to assess the post-thawing motility of ram and minke whale spermatozoa, and that the SQA and HOS test values are significantly correlated in ram spermatozoa. However, sperm concentration and morphologically normal spermatozoa are not assessed accurately by SQA in minke whales. 相似文献
17.
Norihito Yamauchi Hayato Horinouchi Kazuhiko Sakai Kengo Yonemoto Mamoru Satou Takashi Shirakawa 《Journal of General Plant Pathology》2011,77(4):260-262
The race of field isolates of Peronospora farinosa f. sp. spinaciae (Pfs), causal agent of spinach downy mildew, were identified using race-differential cultivars. One isolate was similar to
race Pfs:6. Three isolates were identified as race Pfs:8, the first time the race has been reported in Japan as far as we
know. The differential reaction caused by the other two isolates did not match any known to be caused by races Pfs:1 through
Pfs:11; thus, this strain appears to a new pathogenic strain in Japan. 相似文献
18.
以盒维数法分形分析水稻根系形态特征及初探其与锌吸收积累的关系 总被引：10，自引：0，他引：10
利用特定根盒装土, 培养4个水稻品种(MADHUKAR、IR8192-200、IR26、IR8192-31)植株, 用钉板法结合透明塑料膜固定获得近似原位根系样品, 扫描得到根系的二维平面图像, 以分形理论为基础, 利用盒维数法结合根系图像分形分析程序计算根系构型的分形维数和分形丰度, 比较各品种根系的形态特征, 并对分形参数、根系长度和 植株锌含量间的相关关系做了初步探讨。结果表明, 根系分形维数和分形丰度以MADHUKAR最大, IR8192-200最小, 说明MADHUKAR根系分支多, 在土壤中拓展体积大。分形维数、分形丰度与根系总长度之间均呈明显正相关, 而且根系总长度与分形丰度相关系数高于与分形维数的相关系数。分形维数和分形丰度与植株地上部干重、单位Zn浓度所产出的地上部生物量、地上部Zn吸收总量之间均呈显著正相关, 与地上部Zn浓度呈负相关。水稻根系形态和构型的变化影响植株生长, 影响植株Zn吸收积累及体内Zn的利用效率。盒维数法分形分析模型可用于研究水稻根系形态和构型, 为其提供新方法。 相似文献
19.
Akiyoshi EGASHIRA Nobuhiko YAMAUCHI Keiko TANAKA Chihiro MINE Hitomi OTSUBO Masao MURAKAMI Md. Rashedul ISLAM Misako OHTSUKA Naomi YOSHIOKA Takashi KURAMOTO 《The Journal of reproduction and development》2015,61(6):595-600
The presence of multinucleated blastomeres (MNBs) in embryos is associated with poor developmental
competence in assisted reproductive technologies. This phenomenon is observed not only in humans but also in
other animal species. The purpose of the present study was to investigate the characteristics of embryos with
MNBs (MNB embryos) that could be utilized in embryo transfer. The developmental rate of MNB embryos to the
blastocyst stage (50.8%) was significantly lower than that of normal embryos (73.3%) (P < 0.05). The
clinical pregnancy rates of fresh embryo transfer (ET) using day 2 or day 3 embryos were significantly lower
in MNB embryos (5.1%) compared with normal embryos (24.0%) (P < 0.05). In the case of frozen-thawed ET
using a single vitrified/warmed blastocyst, however, the clinical pregnancy rate of MNB embryos was close to
that of normal embryos (59.1% vs. 52.8%). Thus, the findings of the present study suggest
that the frozen-thawed ET of MNB embryos might improve the potential for implantation followed by successful
pregnancy. 相似文献
20.
Norihito Yamauchi Mamoru Satou Jyuichi Shimazu Takashi Shirakawa Seizo Horiuchi 《Journal of General Plant Pathology》2007,73(1):29-34
Race 2 isolates of Fusarium oxysporum f. sp. lactucae have been recognized as biotin auxotrophs and consequently have restricted growth on Puhalla's minimal medium (MM), which
contains no biotin. Biotin-autotrophic isolates were raised from race 2 isolates through cultural mutation that grew as well
on MM as they did on MM supplemented with biotin. These autotrophs were identical to the parental isolates in pathogenicity
on race differential cultivars of lettuce (Patriot, Banchu Red Fire, and Costa Rica No. 4), and thus were designated as race
2. A vegetative compatibility test indicated that the autotrophic isolates fell into the same vegetative compatibility group
as the parents. Culture filtrates of the autotrophs allowed abundant growth of the parental auxotroph on MM, and, through
a competitive enzyme-binding assay, biotin was detected in the culture filtrates. These results suggest that biotin auxotrophy
in the natural race 2 isolates has no direct relation to pathogenicity, qualitatively defined as physiological race, or to
vegetative compatibility. 相似文献