Molecular evaluation of the incidence of Ehrlichia canis, Anaplasma platys and Babesia spp. in dogs from Ribeirão Preto, Brazil     

Santos F  Coppede JS  Pereira AL  Oliveira LP  Roberto PG  Benedetti RB  Zucoloto LB  Lucas F  Sobreira L  Marins M 《Veterinary journal (London, England : 1997)》2009,179(1):145-148

Canine monocytic ehrlichiosis caused by Ehrlichia canis is endemic in many regions of Brazil. Since thrombocytopenia is a common finding in infected dogs, many clinicians tend to use it as an indication for antibiotic treatment. Polymerase chain reaction (PCR) and nested PCR were used to study the presence of E. canis, Anaplasma platys and Babesia spp. in thrombocytopenic and non-thrombocytopenic dogs from Ribeirão Preto, Brazil. Despite the high prevalence of E. canis infection among thrombocytopenic dogs, 46.7% of the thrombocytopenic dogs studied were either infected with Babesia spp. or A. platys or not infected with any of the three pathogens. There was a high incidence (25.4%) of E. canis infection in non-thrombocytopenic dogs. Although infection with E. canis should be considered in thrombocytopenic dogs, the final diagnosis needs to be confirmed by complementary tests such as blood smears and PCR to avoid the unnecessary use of antibiotics.  相似文献   

Disseminated Mycobacterium avium subsp. avium infection in a Captive Richardson's Ground Squirrel (Spermophilus richardsonii)     

Carles Juan-Sallés  Rui Patrício  Joseba Garrido  Michael M. Garner 《Journal of Exotic Pet Medicine》2009,18(4):306-310

Although mycobacteria have been isolated from rodents, overt mycobacteriosis is rare in any rodent species. A pet adult male Richardson's ground squirrel (Spermophilus richardsonii) died after a short course of alopecia, anorexia, and weight loss. Necropsy and subsequent histopathological examination of tissue samples revealed disseminated granulomatous inflammation involving the lungs, lymph nodes, liver, intestine, adrenal gland, spleen, pleura, and peritoneum. Ziehl-Neelsen stain revealed high numbers of acid-fast bacilli within the cytoplasm of macrophages and multinucleated giant cells. Polymerase chain reaction performed on paraffin-embedded tissues was positive for Mycobacterium avium subsp. avium and negative for M. bovis-tuberculosis complex. Three females housed with this squirrel remained clinically healthy, and mycobacterial cultures of pooled feces from these animals were negative. To the authors' knowledge, this is the second report of disseminated mycobacteriosis in squirrels involving M. avium subsp. avium, with both cases described recently in the Iberian Peninsula.  相似文献   

Use of shrimp protein hydrolysate in Nile tilapia (Oreochromis niloticus, L.) feeds     

Albino Luciani Gonçalves Leal  Patrícia Fernandes de Castro  João Paulo Viana de Lima  Eudes de Souza Correia  Ranilson de Souza Bezerra 《Aquaculture International》2010,18(4):635-646

A 45-day feeding trial was carried out to evaluate the use of shrimp protein hydrolysate (SPH) in diets for Oreochromis niloticus, L. SPH was included in isonitrogenous diets replacing fish meal protein by 0, 5, 10, and 20% and offered to Nile tilapia juveniles (1.7 ± 0.4 g) stocked in 40-L glass aquaria. The inclusion of SPH produced no significant differences (P ≥ 0.05) in final weight, survival, weight gain, average daily gain, specific growth rate, feed conversion ratio, protein efficiency ratio, or apparent net protein utilization. The inclusion of SPH Nile tilapia diets significantly affected (P < 0.05) the final fish body composition. Protein and ash contents decreased and fat content increased slightly with the increase in SPH. This study has demonstrated that SPH is a promising protein feedstuff and could account for as much as 6% of Nile tilapia diets with no adverse effects on growth and nutrient utilization.  相似文献   

Nitrogen nutrition and changes in the chemical attributes of the soil for cultivars of Brachiaria brizantha intercropped with Stylosanthes in different forage systems     

Patrícia Soares Epifanio  Eduardo Da Costa Severiano  Gustavo André Simon  Valdevino Rodrigues Da Silva 《Archives of Agronomy and Soil Science》2020,66(8):1154-1169

ABSTRACT

Nitrogen in the soil-plant system is extremely complex, and any change in soil management or cultivated species can modify its dynamics. To minimize environmental problems and sustainably optimize agricultural production, it is necessary to understand the dynamics of nitrogen in the soil-plant system. Thus, the objective of this study was to evaluate plant nitrogen nutrition and changes in the chemical attributes of the soil for cultivars of Brachiaria brizantha intercropped with Stylosanthes in different forage systems. The experimental design consisted of randomized block experimental design was used, with three replicates. The treatments consisted of forage systems: Piata palisadegrass, Paiaguas palisadegrass, Stylosanthes cv. Campo Grande, row intercropping of Piata palisadegrass with Stylosanthes, mixed intercropping of Piata palisadegrass and Stylosanthes, row intercropping of Paiaguas palisadegrass and Stylosanthes, and mixed intercropping of Paiaguas palisadegrass and Stylosanthes. Evaluations were performed in winter, spring, summer, and autumn in the same plots and over a 2-year period. The intercropping of grasses and legumes positively influences the chemical characteristics of the soil and the nutritional status of the forage by improving the concentration of nutrients. Overall the row seeding method provided better nutrient concentrations. The second year provided higher nitrogen absorption but resulted in greater soil acidification.  相似文献   

Genetic monitoring of broodstocks of the marine shrimp Litopenaeus vannamei in a closed rearing system in Pernambuco,Brazil     

Ana Patrícia Souza De Lima  Andréa Carla Lira Dos Santos  Hozana Leite Dantas  Manoel Adrião Gomes Filho  Rodrigo Maggioni  Maria Raquel Moura Coimbra 《Aquaculture Research》2008,39(13):1461-1466

Loss of genetic variability can be detrimental to a population's survival traits and fitness. These effects are likely to be maximized in shrimp closed broodstock rearing systems where post‐larvae are often produced from crosses of breeders collected from an associated grow‐out farm after mass selection. Longtime broodstock management in closed systems is also expected to lead to reduction in or even complete eradication of genetic variability. The present work aimed at monitoring the genetic variability of a Litopenaeus vannamei hatchery in the state of Pernambuco (Brazil), during three successive replacements, using microsatellite markers. No significant genetic diversity losses have been observed through the values of mean heterozygosity (H_o=0.460 and H_e=0.660 in the first sample; H_o=0.420 and H_e=0.620 in the second sample; and H_o=0.600 and H_e=0.660 in the third sample). However, some alleles appear to have been lost after three replacements. The diversity level was considered to be high and is comparable to those reported for wild populations, suggesting that the original imported founder stock of Brazilian L. vannamei is likely to have had a high genetic diversity, possibly due to multiple origins.  相似文献