Clinicopathological and immunohistochemical description of an intrapelvic hepatoid gland carcinoma in a 14-year-old Teckel dog     

Joelson Jardim  Priscila E. Kobayashi  Patrícia D. Cosentino  Ana Alcaraz  Renée Laufer-Amorim 《The Veterinary quarterly》2018,38(1):9-13

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Social challenge increases cortisol and hypothalamic monoamine levels in matrinxã (Brycon amazonicus)     

Carla Patrícia Bejo Wolkers  Mônica Serra  Elisabeth Criscuolo Urbinati 《Fish physiology and biochemistry》2015,41(6):1501-1508

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Qualitative and quantitative polymerase chain reaction (PCR) for detection of Leishmania in spleen samples from naturally infected dogs     

Solcà Mda S  Guedes CE  Nascimento EG  Oliveira GG  dos Santos WL  Fraga DB  Veras PS 《Veterinary parasitology》2012,184(2-4):133-140

Because infected dogs are widely considered to be the main domestic reservoir for Leishmania infantum (syn Leishmania chagasi) parasites in Brazil, the diagnosis of canine visceral leishmaniasis (CVL) must be made both accurately and promptly. The present study attempted to standardize a conventional polymerase chain reaction (cPCR) protocol for the detection of L. infantum DNA in canine spleen samples. Quantitative PCR (qPCR) technique was used to confirm the presence of Leishmania DNA in the canine spleen fragments. A comparison was made between the efficacies of these molecular diagnostic techniques and conventional parasitological and serological methods. cPCR protocols for spleen samples were standardized using primers that amplify a 145 bp fragment, located at the parasite kinetoplast minicircle. The genus specificity of the cPCR protocol was assessed by its inability to amplify the DNA of other common canine pathogens, such as Ehrlichia canis, Babesia canis, Toxoplasma gondii and Trypanosoma cruzi. cPCR protocol sensitivity was tested by assessing the reaction detection limit, determined to be 10 fg of L. infantum reference strain DNA, which corresponds to a range of 0.03-0.1 parasites per fragment. Standardized cPCR protocol was used to detect the presence of Leishmania in 45 dog spleen samples. Our results showed that 40% of the spleen fragment cultures were positive for Leishmania parasites, 58% of the dog serum samples tested positive using ELISA, and parasite DNA was detected in 44% using qPCR, while 47% of the spleen samples using cPCR. Diagnostic methods performance was assessed and revealed a better degree of ascertainment for cPCR when compared to other diagnostic methods. The sensitivity of ELISA was 83.3%, qPCR was 83.3%, and cPCR was 88.9%; PPV for ELISA was 57.7%, qPCR was 75% and cPCR was 76.2%; the Kappa coefficients were found to be 0.40 (fair) for ELISA, 0.64 (substantial) for qPCR and 0.68 (substantial) for cPCR. In both oligosymptomatic and polysymptomatic dogs, cPCR revealed the better performance analysis when compared to other diagnostic methods. The findings presented herein establish cPCR as the most indicated test to detect Leishmania when compared to the other two diagnostic methods evaluated. Despite the fact that the qPCR protocol provides a highly accurate quantification of parasites when targeting the SSU rRNA gene, this technique does not significantly improve the diagnosis of CVL when compared with the performance of the cPCR protocol, which focused on the kinetoplast minicircle.  相似文献   

Genetic characterisation of antibiotic resistance and virulence factors in vanA-containing enterococci from cattle, sheep and pigs subsequent to the discontinuation of the use of avoparcin     

Ramos S  Igrejas G  Rodrigues J  Capelo-Martinez JL  Poeta P 《Veterinary journal (London, England : 1997)》2012,193(1):301-303

The prevalence of vancomycin resistant-enterococci (VRE) in faecal samples from cattle, sheep and pigs slaughtered for human consumption was evaluated. Enterococci containing the vanA gene were detected in 25.3% and 2.7% of the porcine and ovine samples, respectively, and were identified as Enterococcus faecium. No vanA-containing enterococcal strains were detected in bovine samples. Enterococcal strains with intrinsic vancomycin resistance were detected in seven (9.9%) faecal samples from pigs and in two samples from both cattle and sheep (3.7% and 2.7%, respectively). All vanA-positive isolates from pigs were resistant to tetracycline and erythromycin, and the mobile element Tn916/Tn1545-like transposon was detected in 90.5% of the tetracycline-resistant isolates that contained the tet(M) gene. Although gelatinase and haemolytic activity were not detected, the hyl and cylB virulence genes were found within the VRE strains isolated.  相似文献   

Individual response of growing pigs to sulphur amino acid intake     

Heger J  Krízová L  Sustala M  Nitrayová S  Patrás P  Hampel D 《Journal of animal physiology and animal nutrition》2008,92(1):18-28

Two N balance experiments were conducted to study the individual response of growing pigs to limiting amino acid (AA) intake. Series of fifteen diets with increasing concentration of sulphur amino acids (SAA, Expt 1) or methionine in the presence of excess cystine (Expt 2) were fed sequentially to nine pigs during a 15-day experimental period. The concentration of the AA under test ranged from 50% to 140% of the requirement while other essential AA were given in a 25% excess relative to the limiting AA. N retention was related to the limiting AA intake using rectilinear and curvilinear models. In Expt 1, the quadratic-plateau model fitted the individual data significantly better (p = 0.01) than the linear-plateau model. No difference was found between the two models in Expt. 2, presumably due to the sparing effect of excess cystine on methionine utilization. Exponential, saturation kinetics or four-parameter logistic models fitted to data for all pigs showed that their goodness of fit was similar to those of quadratic-plateau or linear-plateau models. Significant differences (p < 0.05) were found between individual plateau values for N retention within each experiment while the slopes of the regression lines did not significantly differ either in Expt 1 (p = 0.07) or Expt 2 (p = 0.45). There was a positive correlation between the slope and plateau values of the linear-plateau model in Expt 1 (r = 0.74, p = 0.02) but no significant correlation was found in Expt 2 (r = -0.48, p = 0.13). Marginal efficiencies of SAA and methionine utilization derived from the linear-plateau model were 0.43 and 0.65 respectively. Based on linear-plateau and quadratic-plateau models, daily requirements of SAA and methionine for a 50 kg pig were estimated to be 13.0 and 5.9 g and 14.3 and 6.1 g respectively.  相似文献   

Serological survey of Toxoplasma gondii infection in domestic cats from northeastern Portugal   总被引：1，自引：0，他引：1  

Lopes AP  Cardoso L  Rodrigues M 《Veterinary parasitology》2008,155(3-4):184-189

Cats are very important hosts in the epidemiological cycle of Toxoplasma gondii, a zoonotic protozoan parasite that can infect humans and many other animal species worldwide. We report a serological survey of antibodies to T. gondii in domestic cats from northeastern Portugal, by means of the modified agglutination test. Three cats had titres of 20 (3.9%), 18 had titres of 40 (23.7%) and 55 animals had titres of > or =800 (72.4%). Results of three seropositive kittens with less than 4 months were not considered for determining the seroprevalence of infection, which was found to be 35.8% (73/204). Differences in the seroprevalence levels were not statistically significant between males (35.6%) and females (36.0%) or pure non-European (26.7%) and European or mixed-breed cats (39.6%). Animals aged 36-71 months and 72-180 months had the highest seroprevalences of infection, i.e. 51.7% and 51.2%, respectively, which significantly differ from the values observed in cats with 2-11 months (14.6%) and 12-35 months (26.3%). Infection levels were also significantly different between cats that lived totally indoors (7.7%) and those that had access to outdoors (45.4%), as well as between cats living alone (13.8%) and those that had contact with other cats (39.4%). Seroprevalence values in cats fed only commercial canned or dried food (22.9%) and animals whose diet included raw or undercooked viscera and/or meat (53.5%) were also significantly different. Furthermore, considering only 108 cats, differences of seropositivity to T. gondii were significant between feline immunodeficiency virus infected and non-infected animals, but this was not observed for feline leukaemia virus. Age, habitat and diet were identified as risk factors for the feline T. gondii infection by logistic regression analysis. Some control measures are suggested based on these findings.  相似文献   

Characterization of antibiosis to the silverleaf whitefly Bemisia tabaci biotype B (Hemiptera: Aleyrodidae) in cowpea entries     

Patrícia L. Cruz  Edson L. L. Baldin  Maria de Jesus P. de Castro 《Journal of pest science》2014,87(4):639-645

The silverleaf whitefly, Bemisia tabaci (Gennadius) biotype B (Hemiptera: Aleyrodidae), is considered one of the most important pests of cowpea (Vigna unguiculata L. Walp.), limiting the productivity of this crop worldwide. Chemical control is still the main strategy for the management of populations of this insect. However, due to the harmful effects of pesticides on the environment and to humans, less injurious alternatives have been investigated. Along this line, the use of resistant genotypes can be a valuable tool for the control of the silverleaf whitefly. In this paper, we investigate some biological aspects of B. tabaci biotype B confined on 14 genotypes of cowpea. We evaluated the incubation period, egg viability, duration of nymphal stages, total duration of the juvenile phase, instar mortality and total mortality of the immature stage. The genotype MNC 99-541 F21 exhibited antibiosis against the whitefly, prolonging the lifecycle of the insect. The genotypes Canapu, BRS-Urubuquara and TE97-304 G-4 also exhibited antibiosis, causing high nymphal mortality. These results may help in breeding programmes to develop cowpea lines with resistance to B. tabaci biotype B.  相似文献   

Effects of different mydriatics on intraocular pressure,pupil diameter,and ruminal and intestinal motility in healthy sheep     

Alexandre P. Ribeiro  Roberta M. Crivelaro  Pedro Paulo M. Teixeira  Dunia Y. Trujillo  Patrícia J. Guimarães  Wilter Ricardo R. Vicente  Bianca da C. Martins  José Luiz Laus 《Veterinary ophthalmology》2014,17(6):397-402

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Testicular shape,scrotal skin thickness and testicular artery blood flow changes in bulls of different ages     

Luís Afonso Marques Claus  Flávio Antônio Barca Junior  Celso Koetz Junior  Gabriel Ribas Pereira  Patrícia da Cruz Fávaro  Fabio Procópio Ferreira  Victor Hugo Gonçalves Galdioli  Marcelo Marcondes Seneda  Edson Luís de Azambuja Ribeiro 《Reproduction in domestic animals》2021,56(7):1034-1039

The aim of this study was to compare the biometric testicular characteristics, skin thickness and haemodynamics of the testicular artery of 12- and 24-month-old bulls using Doppler ultrasonography, the study was conducted using 48 indicus-taurus animals. The scrotal circumference (SC) and biometry characteristics of the bulls were measured to calculate the testicular volume. Doppler ultrasonography was used to obtain the haemodynamic values of the testicular artery. The skin thickness and volume were lower (p<.01) in the younger bulls (12 months:4.68 ± 0.68 mm; 168.76 ± 47.96 cm³) versus 24 months (5.05 ± 0.89; 499.73 ± 129.24 cm³) animals (p<.01). During diastole, mean velocity was lower in the 12 months (7.98 ± 3.83) than in the 24 months (11.37 ± 4.15) animals (p <.05). The 12-month-old animals had higher pulsatility and resistivity indices (0.49 ± 0.02; 0.51 ± 0.20) compared to the 24-month-old animals (0.32 ± 0.16; 0.40 ± 0.15) (p < .05). The final testicular end velocity was lower in animals with long/moderate-shaped (L/M) (7.31 ± 2.91) than in those moderate/oval-shaped (M/O) (11.48 ± 3.88) testicles (p < .05). Animals with L/M testes presented higher pulsatility values and resistivity indices (0.51 ± 0.05; 0.55 ± 0.04) compared to animals with M/O shape (0.29 ± 0.20; 0.36 ± 0.15). We showed that the blood flow of the supra testicular artery between the two evaluated ages differed, and that 24-month-old bulls presented better thermoregulation capacity. Animals with a long/moderate testicular format presented a greater vascular resistance, which was imposed on the blood flow due to the anatomical differences in the testicular artery, resulting in lower velocity, and indicating better heat dissipation in this format.  相似文献   

Bats,pangolins, minks and other animals - villains or victims of SARS-CoV-2?     

do Vale  Beatriz  Lopes  Ana Patrícia  Fontes  Maria da Conceição  Silvestre  Mário  Cardoso  Luís  Coelho  Ana Cláudia 《Veterinary research communications》2021,45(1):1-19

Veterinary Research Communications - Coronavirus disease-19 (COVID-19) is caused by the severe acute Respiratory syndrome coronavirus-2 (SARS-CoV-2), which has become unstoppable, spreading rapidly...  相似文献