Comparative study on free amino acid composition of wild edible mushroom species     

Ribeiro B  Andrade PB  Silva BM  Baptista P  Seabra RM  Valentão P 《Journal of agricultural and food chemistry》2008,56(22):10973-10979

A comparative study on the amino acid composition of 11 wild edible mushroom species (Suillus bellini, Suillus luteus, Suillus granulatus, Tricholomopsis rutilans, Hygrophorus agathosmus, Amanita rubescens, Russula cyanoxantha, Boletus edulis, Tricholoma equestre, Fistulina hepatica, and Cantharellus cibarius) was developed. To define the qualitative and quantitative profiles, a derivatization procedure with dabsyl chloride was performed, followed by HPLC-UV-vis analysis. Twenty free amino acids (aspartic acid, glutamic acid, asparagine, glutamine, serine, threonine, glycine, alanine, valine, proline, arginine, isoleucine, leucine, tryptophan, phenylalanine, cysteine, ornithine, lysine, histidine, and tyrosine) were determined. B. edulis and T. equestre were revealed to be the most nutritional species, whereas F. hepatica was the poorest. The different species exhibited distinct free amino acid profiles. The quantification of the identified compounds indicated that, in a general way, alanine was the major amino acid. The results show that the analyzed mushroom species possess moderate amino acid contents, which may be relevant from a nutritional point of view because these compounds are indispensable for human health. A combination of different mushroom species in the diet would offer good amounts of amino acids and a great diversity of palatable sensations.  相似文献   

Effect of Marcela Extract (Achyroclines satureiodes) on the Shelf Life of Minced Tilapia (Oreochromis niloticus) Sausages     

Silvério Nepomuceno de Lima  Cintia Cristina de Oliveira  Edevane Alves Castilho  Karina Aparecida Damasceno  Patrícia Leal de Almeida  Simone Cristina de Oliveira 《Journal Of Aquatic Food Product Technology》2017,26(2):140-147

The effect of marcela extract (ME) on the shelf life of minced tilapia sausages was evaluated in this study. The level of phenolic compounds in the ME was 114.1 ± 2.9 mg GAE/g extract, and the DPPH free radical scavenging activity was 94.95 ± 1.34%. The ME protected the samples from color changes during storage. The addition of 0.5 and 0.75% ME significantly decreased lipid oxidation and microbial growth. The ME did not impact the sausages’ sensory quality, and the addition of 0.75% prevented sensory rejection during storage.  相似文献   

Gram-negative bacterial diversity and evidence of international clones of multidrug-resistant strains in zoo animals     

João Pedro Rueda FURLAN  Micaela Santana RAMOS  Fábio Parra SELLERA  Irys Hany Lima GONZALEZ  Patrícia Locosque RAMOS  Eliana Guedes STEHLING 《Integrative zoology》2024,19(3):417-423

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Impact on C and N dynamics of simultaneous application of pig slurry and wheat straw, as affected by their initial locations in soil     

Celso Aita  Sylvie Recous  Regina Helena Osmari Cargnin  Laura Patrícia da Luz  Sandro José Giacomini 《Biology and Fertility of Soils》2012,48(6):633-642

The joint management of animal manures and plant biomass as straw on agricultural soils may be a viable option for reducing the environmental impacts associated with livestock production and recycling nutrients efficiently. To investigate this option, an incubation in controlled conditions examined how the simultaneous addition of ¹⁵N-labeled pig slurry and ¹³C-labeled wheat straw, either on the soil surface or incorporated into the soil, affected the mineralization of C from the organic materials and the soil N dynamics. Samples from a typic hapludalf were incubated for 95 days at 25°C with eight treatments: unamended soil (S), wheat straw left on the soil surface (Ws), wheat straw incorporated in the soil (Wi), pig slurry on the soil surface (Ps), pig slurry incorporated in the soil (Pi) and three combinations of the two amendments: Pi?+?Ws, Pi?+?Wi, and Ws?+?Ps. Carbon dioxide and ¹³CO₂ emissions and soil N content were measured throughout the incubation. Pig slurry stimulated the decomposition of straw C only when wheat straw and pig slurry were left together on the soil surface. Incorporation of both wheat straw and pig slurry did not modify straw C mineralization when compared to straw incorporation alone but this promoted a higher rate of N immobilization. The results suggest that when pig slurry is used in field under no-till conditions, the best strategy to preserve environmental quality with regard to CO₂ emissions would be to apply pig slurry underneath the crop residues.  相似文献   

Chromomycin A2 Induces Autophagy in Melanoma Cells     

Larissa Alves Guimar?es  Paula Christine Jimenez  Thiciana da Silva Sousa  Hozana Patrícia S. Freitas  Danilo Damasceno Rocha  Diego Veras Wilke  Jesús Martín  Fernando Reyes  Otília Deusdênia Loiola Pessoa  Letícia Veras Costa-Lotufo 《Marine drugs》2014,12(12):5839-5855

The present study highlights the biological effects of chromomycin A2 toward metastatic melanoma cells in culture. Besides chromomycin A2, chromomycin A3 and demethylchromomycin A2 were also identified from the extract derived from Streptomyces sp., recovered from Paracuru Beach, located in the northeast region of Brazil. The cytotoxic activity of chromomycin A2 was evaluated across a panel of human tumor cell lines, which found IC₅₀ values in the nM-range for exposures of 48 and 72 h. MALME-3M, a metastatic melanoma cell line, showed the highest sensitivity to chromomycin A2 after 48h incubation, and was chosen as a model to investigate this potent cytotoxic effect. Treatment with chromomycin A2 at 30 nM reduced cell proliferation, but had no significant effect upon cell viability. Additionally, chromomycin A2 induced accumulation of cells in G₀/G₁ phase of the cell cycle, with consequent reduction of S and G₂/M and unbalanced expression of cyclins. Chromomycin A2 treated cells depicted several cellular fragments resembling autophagosomes and increased expression of proteins LC3-A and LC3-B. Moreover, exposure to chromomycin A2 also induced the appearance of acidic vacuolar organelles in treated cells. These features combined are suggestive of the induction of autophagy promoted by chromomycin A2, a feature not previously described for chromomycins.  相似文献   

Phosphite determination in fertilizers after online sequential sample preparation in a flow injection system     

Dametto PR  Franzini VP  Gomes Neto JA 《Journal of agricultural and food chemistry》2007,55(15):5980-5983

A flow injection spectrophotometric system is proposed for phosphite determination in fertilizers by the molybdenum blue method after the processing of each sample two times on-line without and with an oxidizing step. The flow system was designed to add sulfuric acid or permanganate solutions alternately into the system by simply displacing the injector-commutator from one resting position to another, allowing the determination of phosphate and total phosphate, respectively. The concentration of phosphite is obtained then by difference between the two measurents. The influence of flow rates, sample volume, and dimension of flow line connecting the injector-commutator to the main analytical channel was evaluated. The proposed method was applied to phosphite determination in commercial liquid fertilizers. Results obtained with the proposed FIA system were not statistically different from those obtained by titrimetry at the 95% confidence level. In addition, recoveries within 94 and 100% of spiked fertilizers were found. The relative standard deviation (n = 12) related to the phosphite-converted-phosphate peak alone was 相似文献   

Variable selection, outlier detection, and figures of merit estimation in a partial least-squares regression multivariate calibration model. A case study for the determination of quality parameters in the alcohol industry by near-infrared spectroscopy     

Valderrama P  Braga JW  Poppi RJ 《Journal of agricultural and food chemistry》2007,55(21):8331-8338

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Echocardiographic evaluation including tissue Doppler imaging in New Zealand white rabbits sedated with ketamine and midazolam     

Fontes-Sousa AP  Moura C  Carneiro CS  Teixeira-Pinto A  Areias JC  Leite-Moreira AF 《Veterinary journal (London, England : 1997)》2009,181(3):326-331

Limited data are available on the use of more recent echocardiographic parameters in the rabbit. Echocardiographic examination, including conventional echocardiography and tissue Doppler imaging (TDI), was performed on 26 male New Zealand white rabbits under ketamine–midazolam sedation. Particular emphasis was placed on the more recent systolic and diastolic parameters, such as myocardial performance index (Tei index) and mitral annular motion (from septal and lateral sides of the left ventricle) obtained using pulsed TDI.Parameters that assessed systolic and diastolic function (fractional shortening, Tei index, and maximal mitral E- and A-wave velocities) were comparable to those reported in the literature for rabbits in the awake state. The less cardiodepressive anaesthetic protocol could offer a good alternative in performing echocardiographic evaluation whenever such caution is necessary. TDI is feasible in healthy rabbits and potentially suitable for the investigation of left ventricle systolic and diastolic function.  相似文献   

Quantitation of nine organic acids in wild mushrooms   总被引：3，自引：0，他引：3  

Valentão P  Lopes G  Valente M  Barbosa P  Andrade PB  Silva BM  Baptista P  Seabra RM 《Journal of agricultural and food chemistry》2005,53(9):3626-3630

The organic acids composition of six wild edible mushroom species (Amanita caesarea, Boletus edulis, Gyroporus castaneus, Lactarius deliciosus, Suillus collinitus, and Xerocomus chrysenteron) was determined by an HPLC-UV detector method. The results showed that all of the samples presented a profile composed of at least five organic acids: citric, ketoglutaric, malic, succinic, and fumaric acids. Several samples also contained oxalic, ascorbic, quinic, and shikimic acids. In a general way, the quantitation of the identified compounds indicated that malic acid, followed by the pair citric plus ketoglutaric acids, were the main compounds in the analyzed species, with the exception of A. caesarea, in which malic and ascorbic acids were the most abundant compounds. The relative amounts and the presence/absence of each identified compound may be useful for the differentiation of the species.  相似文献   

Antioxidant properties of prepared blueberry (Vaccinium myrtillus) extracts   总被引：3，自引：0，他引：3  

Faria A  Oliveira J  Neves P  Gameiro P  Santos-Buelga C  de Freitas V  Mateus N 《Journal of agricultural and food chemistry》2005,53(17):6896-6902

A blueberry extract (A) and two anthocyanin-derived extracts (B and C) were prepared. The contents of polyphenols, flavonoids, anthocyanins, and anthocyanin-derived pigments of the extracts were determined for the first time. The pigment profile of blueberry extract A corresponded to 15 anthocyanins, whereas extract B was mainly composed of anthocyanin-pyruvic acid adducts of the blueberry original anthocyanins and extract C was mainly composed of the respective vinylpyranoanthocyanin-catechins (portisins). The extracts' abilities to inhibit lipid peroxidation, induced by 2,2'-azobis(2-methyl-propanimidamide) dihydrochloride in a liposomal membrane system were examined. The antioxidant capacities of the extracts were evaluated through monitoring oxygen consumption and by measuring the formation of conjugated dienes. All of the extracts provided protection of membranes against peroxyl radicals by increasing the induction time of oxidation. This effect increased with the polyphenol content and with the structural complexity of the anthocyanin-derived pigments of the extracts. The pigments present in extract C seemed to induce a higher protection of the liposome membranes toward oxidation. In addition, the antiradical properties and the reducing power of the extracts were determined by using DPPH and FRAP methods, respectively. The results from these assays were in agreement with those obtained with the liposome membranes.  相似文献