Residues of polychlorinated biphenyl in products from poultry fed Aroclor 1254   总被引：3，自引：0，他引：3  

R H Teske  B H Armbrecht  R J Condon  H J Paulin 《Journal of agricultural and food chemistry》1974,22(5):900-904

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Relationships between electrolyte leakage from Pyrus communis and virulence of Erwinia amylovora     

M.N. Brisset  J.P. Paulin   《Physiological and Molecular Plant Pathology》1991,38(6)

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Acibenzolar-S-methyl Induces the Accumulation of Defense-related Enzymes in Apple and Protects from Fire Blight   总被引：4，自引：0，他引：4  

Marie-Noëlle Brisset  Sophie Cesbron  Sherman V. Thomson  Jean-Pierre Paulin 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(6):529-536

Acibenzolar-S-methyl (Novartis) is a chemical inducer of systemic acquired resistance in several annual plants. The ability of this novel chemical to induce resistance was studied in a perennial plant (apple) affected by fire blight caused by Erwinia amylovora. Acibenzolar-S-methyl (100 and 200mg/l active ingredient) protected Golden Delicious seedlings, scions and trees from artificial infection when applied before inoculation. The protection of apple seedlings was similar to the protection obtained with the standard for fire blight control, plantomycin (100mg/l streptomycin sulfate), applied immediately before inoculation. The mean levels of control in scions in the greenhouse and in trees in orchards were approximately 69% and 50%, respectively. The protection of apple seedlings was constantly associated with the activation of two families of defense-related enzymes, peroxidases and -1,3-glucanases. Accumulation of both enzymes was induced locally in treated leaves and systemically, especially for -1,3-glucanases, in upper untreated leaves, and was sustained for at least 17 days. These results suggest that acibenzolar-S-methyl promotes induced systemic resistance in apple by increasing defense-related compounds. This chemical could provide a new approach of control of fire blight but its practical use needs further investigation.  相似文献   

Response of living tissues of Pinus sylvestris to the saprotrophic biocontrol fungus Phlebiopsis gigantea     

Sun H  Paulin L  Alatalo E  Asiegbu FO 《Tree physiology》2011,31(4):438-451

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Fungal arthritis due to infection by Candida famata in a horse     

CB RILEY  JV YOVICH  JP ROBERTSON  FL O''HARA 《Australian veterinary journal》1992,69(3):65-66

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Pathology of experimentally-induced, acute toxoplasmosis in macropods   总被引：1，自引：0，他引：1  

GL REDDACLIFF  WJ HARTLEY  JP DUBEY  DW COOPER 《Australian veterinary journal》1993,70(1):4-6

SUMMARY Thirteen Tammar wallabies (Macropus eugenii) were dosed orally with 500, 1000 or 10 000 oocysts of Toxoplasma gondii, as part of a vaccination trial. Eleven animals died of acute toxoplasmosis 9 to 15 days after challenge. The lesions were similar in all animals, consisting of foci of necrosis and inflammation in the intestines, lymphoid tissue, adrenal cortex, heart, skeletal muscle and brain, and severe generalised pulmonary congestion and oedema. Free and intracellular tachyzoites of Toxoplasma were associated with the lesions. The remaining 2 animals had shown no signs of disease when euthanased four months after challenge. Small, focal, non-suppurative inflammatory lesions were seen in brain, heart and skeletal muscle of these animals and chronic Toxoplasma infection was confirmed by mouse inoculation.  相似文献   

PCR cloning, DNA sequencing and phylogenetic analysis of a xylanase gene from the phytopathogenic fungusAscochyta pisiLib.     

P.S. Lübeck  L. Paulin  Y. Degefu  M. Lübeck  I. Alekhina  S.A. Bulat  D.B. Collinge 《Physiological and Molecular Plant Pathology》1997,51(6):377-389

A gene encoding a xylanase,xyl1, was isolated from the phytopathogenic fungusAscochyta pisiLib. by PCR cloning using degenerate primers. DNA sequence analysis revealed an open reading frame of 736 bp interrupted by an intron of 55 bp. The ORF encodes a predicted protein of 227 amino acids. The precise splicing site of the intron was identified from the sequence of a PCR product obtained using the same degenerated primers on a cDNA template. The cDNA product and a northern blot demonstrated that the gene is transcribed into mRNA when the fungus is cultured in media containing xylan as sole carbon source. The Neighbour-Joining method using the Clustal W(1.5) program demonstrated that theA. pisixylanase is a member of the family 11 glycosyl hydrolases, and that this family represents at least five phylogenetically consistent groups. The family 11 glycosyl hydrolases can be linked with family 10 glycosyl hydrolyses through bifunctional enzymes fromRuminococcus flavefaciensand, to a lesser extentNeocallimastix patriciarum.  相似文献   

Ejaculation does not contribute to the stress response to electroejaculation in sheep          下载免费PDF全文

S Abril‐Sánchez  A Freitas‐de‐Melo  JP Damián  J Giriboni  A Villagrá‐García  R Ungerfeld 《Reproduction in domestic animals》2017,52(3):403-408

Electroejaculation procedures (EEPs) provoke stress; nevertheless, ejaculation produces physiological changes similar as those usually used to measure stress responses. The application of EEP to animals that cannot ejaculate—as ewes—may be useful to discriminate the responses induced by ejaculation from those provoked by EEP. The aim was to determine the stress response to EEP in rams and ewes. The EEPs were applied to 10 rams and 10 ewes during the non‐breeding season, and the number of vocalizations, the heart rate, rectal temperature, serum cortisol concentration, biochemical and haematological parameters were measured. Overall, EEP provoked increases in cortisol concentration, glycaemia, rectal temperature and concentration of creatine kinase (all them: p < .0001) as well as relative concentration of granulocytes (p = .003) and absolute granulocyte concentration (p = .0002) in both, rams and ewes. Heart rate, relative concentration of lymphocytes (p = .001), haematocrit (p = .02) and haemoglobin (p = .045) decreased in animals from both genders after EEP. Besides, cortisol (p < .0001), rectal temperature (p = .002) and glycaemia (p = .001) were greater in ewes than rams, and creatine kinase also tended to be greater in ewes than rams (p = .054). On the other hand, the number of animals that vocalized (p = .006), white blood cells (p = .02) and absolute lymphocytes (p = .02) were greater in rams than ewes. The general trends show a similar pattern of stress responses in animals from both genders. Therefore, we concluded that ejaculation does not contribute to the stress response provoked by the EEP. This procedure also provokes muscular damage and probably pain.  相似文献   

Off-axis crustal thickness across and along the east pacific rise within the MELT area   总被引：1，自引：0，他引：1  

JP Canales  RS Detrick  S Bazin  AJ Harding  JA Orcutt 《Science (New York, N.Y.)》1998,280(5367):1218-1221

Wide-angle seismic data along the Mantle Electromagnetic and Tomography (MELT) arrays show that the thickness of 0.5- to 1. 5-million-year-old crust of the Nazca Plate is not resolvably different from that of the Pacific Plate, despite an asymmetry in depth and gravity across this portion of the East Pacific Rise. Crustal thickness on similarly aged crust on the Nazca plate near a magmatically robust part of the East Pacific Rise at 17 degrees15'S is slightly thinner (5.1 to 5.7 kilometers) than at the 15 degrees55'S overlapping spreading center (5.8 to 6.3 kilometers). This small north-south off-axis crustal thickness difference may reflect along-axis temporal variations in magma supply, whereas the across-axis asymmetry in depth and gravity must be caused by density variations in the underlying mantle.  相似文献   

Twenty years of agronomic evaluation of wild cocoa trees (Theobroma cacao L.) from French Guiana     

Ph. Lachenaud  D. Paulin  M. Ducamp  J.-M. Thevenin 《Scientia Horticulturae》2007

Almost 500 clones of wild cocoa trees (Theobroma cacao L.) grown from pods collected in 1987 from wild mother-trees in the Camopi and Tanpok river basins (southeastern French Guiana) have been distributed in around fifteen cocoa producing countries since 1988. The name of those clones always bears the GU prefix (for “Guyane”, i.e. French Guiana). All the germplasm of the same geographical origin present in the CIRAD collection at Paracou-Combi (Sinnamary, French Guiana), i.e. more than 1600 trees, has been abundantly studied for its morphological characterization, its agronomic assessment or its genetic diversity. Other assessment work, primarily on resistance to certain diseases, has been carried out by CIRAD in Montpellier, or in various countries by other organizations.  相似文献