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991.
992.
993.
Two Methods of Vitrification Followed by In Vitro Culture of the Ovine Ovary: Evaluation of the Follicular Development and Ovarian Extracellular Matrix
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FT Bandeira AA Carvalho SV Castro LF Lima DA Viana JSAM Evangelista MJS Pereira CC Campello JR Figueiredo APR Rodrigues 《Reproduction in domestic animals》2015,50(2):177-185
The aim of this study was to evaluate the influence of two vitrification techniques on the extra cellular matrix (ECM) and ovarian follicular development. The ovarian cortex was fragmented (9 mm3) and divided into six groups, viz. fresh control, cultured control, vitrified by the Ovarian Tissue Cryosystem (OTC) method, conventional solid surface vitrification (SSV) method, OTC/cultured and SSV/cultured. Follicles from all the fragments were analysed for morphology, development and viability. The ECM was evaluated based on the condition of collagen and reticular fibres and the immunolocalization of type I collagen and fibronectin. After 7 days of culture, the tissue vitrified by OTC revealed a higher percentage (p < 0.05) of morphologically normal (30.66%) and viable (60.00%) follicles when compared with those vitrified using the SSV technique (21.33% and 23.00%). In all the fragments cultured, regardless of the vitrification method, a significantly higher percentage of developing follicles was observed when compared with the non‐cultured tissue. Analysis of the type I collagen showed increased immunostaining after the in vitro culture in the vitrified fragments. In conclusion, the OTC is better for preserving the follicular viability and morphology and maintaining the integrity of the extracellular matrix components of the ovine ovary. 相似文献
994.
To evaluate the influence of irrigation and fertilization on the productivity ofE. globulus a field experiment was started in 1986 in Central Portugal. The nutrients accumulated in the biomass and their allocation
to the various biomass components as well as the changes in soil chemical characteristics were also followed. Irrigation and
fertilization resulted in a significant increase of biomass production during the first 2 yr. A similar trend occurred with
the net primary production and leaf Litterfall. The differences between treatments in the amounts of accumulated nutrients
in the aboveground biomass were similar to those of biomass accumulation. However, there was a small but consistent effect
related to the average concentration of nutrients in the plant tissues. This effect occurred essentially in the leaves and
branches. The concentration of N and P in the leaf Litterfall was higher in the fertilized treatments than in the others.
Fertilization alone induced a slight increase in the soil pH values, C content, exchangeable Ca and available P levels. Such
increase was more pronounced in the fertilized with dripping irrigation treatment. This was due to the accumulation of nutrients
in the wetted soil volume. 相似文献
995.
Bruna Pereira de Souza Felipe Paolinelli de Carvalho Marcelo Ehlers Loureiro Walas Permanhane Sturião 《Journal of plant nutrition》2020,43(16):2455-2465
AbstractThis research aims to evaluate the impact of nitrogen deprivation and water stress on gas exchange and chlorophyll fluorescence in young plants of five cultivars of Arabic coffee. A factorial experiment 5 (cultivars) × 3 (treatments: control without stress, water stress of ?1.5?MPa and stress of N – 0.0?mmol L?1 N) was carried out in a complete randomized block design with three replicates. Before being submitted to the treatments, the plants were grown in a greenhouse for 240?days, and then transferred to a growth chamber under controlled conditions. Subsequently, after the experimental period of 96?h we measured photosynthetic rate (A), stomatal conductance to water vapor (gs), transpiratory rate (E), internal and external carbon ratio (Ci/Ca), water use efficiency (A/E), electron transport rate (ETR), actual quantum yield of PS II electron transport (φFSII), and maximum photochemical efficiency of PS II (Fv/Fm). Water stress reduced A, gs, E, A/E, ETR, φFSII, and Fv/Fm. The nitrogen deficiency reduced ETR, φFSII, and Fv/Fm. Under short-term water stress Catuaí Vermelho maintain the A values due to better stomatal control, reduced water lost by transpiration (E) and better water use efficiency A/E, while Mundo Novo and Acauã show lower damage to Fv/Fm. Short-term nitrogen stress has low impact on A of young plants of Coffea arabica cultivars with adequate N-nutrition. 相似文献
996.
Benidire Leila Pereira Sofia I. A. Naylo Ahmed Castro Paula M. L. Boularbah Ali 《Journal of Soils and Sediments》2020,20(2):1003-1017
Journal of Soils and Sediments - Mining areas are low-quality habitats for macro- and microorganisms’ development, mainly due to the degradation of the soil quality by metal pollution. The... 相似文献
997.
Gomez Hector Alonzo Gomez Marques Marcia Ortiz Mayo Borges Cristine Vanz Minatel Igor Otavio Monteiro Gean Charles Ritschel Patricia Silva Zanus Mauro Celso Diamante Marla Silvia Kluge Ricardo Alfredo Lima Giuseppina Pace Pereira 《Plant foods for human nutrition (Dordrecht, Netherlands)》2020,75(2):258-264
Plant Foods for Human Nutrition - Some biogenic amines (BAs) are used as quality markers in grape-derived products. The prolife of 9 BAs was determined in juices and wines elaborated from hybrid... 相似文献
998.
Francielly de Cássia Pereira Adriano Teodoro Bruzi José Wilacildo de Matos Breno Alvarenga Rezende Luis Claudio Prado José Airton Rodrigues Nunes 《Plant Breeding》2017,136(5):679-687
The goal of this study was to verify the influence of the population effect in the estimates of genetic and phenotypic components and to identify the best soybean progeny or lines in a commercial soybean breeding programme. We evaluated 292 populations for grain yield and absolute maturity during three agricultural years. To quantify the efficiency of the inclusion of the population's effect in the model, we estimated genetic gain with the selection, the Spearman's correlation, the coincidence index, the realized gain and correlated response of selected genotypes with and without the effect of the population. It was found that the variance components, heritability and coefficient of experimental variation were better estimated when the effect of the population was included, providing greater gain with selection for grain yield and absolute maturity. Coincidence and ranking among the selected progeny with and without the effect of the population are of greater magnitude in more advanced inbreeding generations and at higher percentage of selected progeny. The use of the population effect has greater importance in earlier generations of inbreeding. 相似文献
999.
Eduardo S. Mendes Carlos Fonseca Sara F. Marques Daniela Maia Maria João Ramos Pereira 《Landscape Ecology》2017,32(2):295-311
Context
The conversion of natural environments into agricultural land has profound effects on the composition of the landscape, often resulting in a mosaic of human-altered and natural habitats. The response to these changes may however vary among organisms. Bats are highly vagile, and their requirements often imply the use of distinct habitats, which they select responding to both landscape and local features.Objectives
We aimed to identify which features influence bat richness and activity within Baixo Vouga Lagunar, a heterogeneous landscape located on the Central-North Portuguese coast, and to investigate if that influence varies across a gradient of focal scales.Methods
We sampled bats acoustically, while simultaneously sampling insects with light traps. We assessed the relationships between species richness, bat activity, and activity of eco-morphological guilds with landscape and local features, across four scales.Results
Our results revealed both scale- and guild-dependent responses of bats to landscape and local features. At broader scales we found positive associations between open-space foraging bats and habitat heterogeneity and between edge-space foraging bats and greater edge lengths. Woodland cover and water availability at an intermediate scale and weather conditions and insect abundance at a local scale were the factors that mostly influenced the response variables.Conclusions
Globally, our results suggest that bats are sensitive to local resource availability and distribution, while simultaneously reacting to landscape features acting at coarser scales. Finally, our results suggest that the responses given by bats are guild-dependent, and some habitats act as keystone structures for bats within this mosaic.1000.
Transport of juvenile dusky grouper Epinephelus marginatus under different packing densities: Metabolic and haematological responses
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Paola Milena Pereira‐Cardona Viviana Lisboa Luis André Luz Barbas Ricardo Berteaux Robaldo 《Aquaculture Research》2017,48(10):5356-5362
The aim of this study was to investigate a suitable packing density for the transport of juvenile dusky grouper, Epinephelus marginatus, based on the evaluation of stress responses in blood. After acclimation, fish were placed in plastic bags and transported for 8 hr on paved road at densities of 28, 45 and 64 g/L. Water quality was monitored before and after transport. Blood was collected before, upon arrival (0 hr), after 2 and 24 hr of transport. Plasma cortisol, blood glucose, partial pressures of O2 (pO2) and CO2 (pCO2), blood pH and HCO3? were evaluated. Blood smears were prepared for the verification of leucocyte profile and neutrophils:lymphocyte ratio (N:L). Blood pCO2, pH and HCO3? increased significantly after transport for all treatments compared with pre‐transport. Glucose levels increased at the higher density whereas no effects were observed on plasma cortisol and pO2 levels. Upon arrival, all treatments showed lymphopenia and neutrophilia which increased N:L ratio. Although lymphopenia was observed in higher densities until 2 hr after transport, haematological parameters were fully restored within 24 hr post transport. Furthermore, no mortalities were observed throughout the experimental period. Based on the transient physiological changes observed in this study, juvenile dusky grouper can be safely transported in plastic bags for 8 hr at a density of up to 64 g/L. 相似文献