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11.
Allelopathy, secondary metabolite-mediated plant-to-plant interaction, is gaining application in current agricultural science as well as in invasion ecology. However, the role of sorption to soil in modifying the bioavailability of components in complex allelochemical mixtures is still obscure. Hence, the role of preferential sorption to soil in altering the chemical composition of plant exudates was studied in a silt loam soil using representative mixtures of plant phenolic acids, namely, hydroxybenzoic acid, vanillic acid, coumaric acid, and ferulic acid. The experiment was conducted using a batch equilibration technique, and data were fitted to a Freundlich isotherm. The concentration-dependent sorption coefficient (K(d)) at 10 microg mL(-)(1) was used to assess the sorption affinity of phenolic acids across different systems. Along with solid phase dissolution, all of the phenolic acids exhibited strong site-specific sorption, as evident from their nonlinear isotherms. Removal of organic matter substantially decreased the sorption affinity of all phenolic acids. Direct competition for sorption sites was observed even at low concentrations of phenolic acids. The K(d) of hydroxybenzoic acid was decreased more than 90% in the presence of coumaric acid. About 95% of sorbed vanillic acid was displaced into the soil solution in the presence of ferulic acid. Hydroxybenzoic acid did not affect the sorption affinity of other phenolic acids significantly, whereas ferulic acid showed low displacement by other phenolic acids. The displacement pattern indicated directional sorption of phenolic acids with -OH and -COOH groups. Soil organic matter was associated with preferential sorption. This is the first study to elucidate competitive sorption characteristics of plant secondary metabolites in soil matrix. The results demonstrate that preferential sorption to soil can alter the availability of plant exudates in mixtures and thus may mediate their phytotoxic effects. 相似文献
12.
Trent W. Biggs Prasanta K. Mishra Hugh Turral 《Agricultural and Forest Meteorology》2008,148(10):1585-1597
The long-term probability of soil moisture stress in rainfed crops was mapped at 0.5° resolution over the Krishna River basin in southern India (258,948 km2). Measurements of actual evapotranspiration (Ea) from 90 lysimeter experiments at four locations in the basin were used to calibrate a non-linear regression model that predicted the combined crop coefficient (KcKs) as a function of the ratio of seasonal precipitation (P) to potential evapotranspiration (Ep). Crops included sorghum, pulses (mung bean, chickpea, soybean, pigeonpea) and oilseeds (safflower and sunflower). Ep was calculated with the Penman–Monteith equation using net radiation derived from two methods: (1) a surface radiation budget calculated from satellite imagery (EpSRB) and (2) empirical equations that use data from meteorological stations (EpGBE). The model of Ks as a function P/Ep was combined with a gridded time series of precipitation (0.5° resolution, 1901–2000) and maps of EpSRB to define the probability distributions of P, P/Ep and Ks for sorghum at each 0.5° cell over the basin. Sorghum, a C4 crop, had higher Ea and Ks values than the C3 plants (oilseeds, pulses) when precipitation was low (P < 1 mm d−1) but lower maximum Ea rates (3.3–4.5 mm d−1) compared with C3 crops (oilseeds and pulses, 4.3–4.9 mm d−1). The crop coefficient under adequate soil moisture (Kc) was higher than the FAO-56 crop coefficients by up to 56% for oilseeds and pulses. The seasonal soil moisture coefficient (Ks) for sorghum ranged from 1.0 under high rainfall (July–October) to 0.45 in dry seasons (November–March), showing strong soil moisture controls on Ea. EpSRB calculated at the lysimeter stations was 4–20% lower than EpGBE, with the largest difference in the dry season. Kc derived from EpSRB was only slightly (2–4%) higher than Kc derived from EpSRB, because the maximum Ea occurred during the monsoon when the differences between EpSRB and EpGBE were small. Approximately 20% of the basin area was expected to experience mild or greater soil moisture stress (Ks < 0.80) during the monsoon cropping season 1 year in every 2 years, while 70% of the basin experienced mild or greater stress 1 year in 10. The maps of soil moisture stress provide the basis for estimating the probability of drought and the benefits of supplemental irrigation. 相似文献
13.
Azita Khorsandi Dai Shi Andrea K. Stone Aarti Bhagwat Yuping Lu Caishuang Xu Prem Prakash Das Brittany Polley Leonid Akhov Jessica Gerein Xiumei Han Pankaj Bhowmik L. Irina Zaharia James D. House Nandhakishore Rajagopalan Takuji Tanaka Darren R. Korber Michael T. Nickerson 《Cereal Chemistry》2024,101(1):131-143
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Avinash Talukdar Ashutosh Dharmendra Deo Narrotam Prasad Sahu Parimal Sardar Muhammad Aklakur Vungarala Harikrishna Satya Prakash Nazeema Shamna Prasanta Jana 《Aquaculture Nutrition》2021,27(1):77-90
A 60‐day feeding trial was conducted to illustrate the effect of dietary protein levels on the growth and physio‐metabolic responses of juvenile Litopenaeus vannamei reared in inland saline water (ISW). Six isoenergetic (15 MJ/kg) and isolipidic (60 g/kg) diets with graded level of crude protein viz., 240 (T240), 260 (T260), 280 (T280), 300 (T300), 320 (T320) and 340 (T340) g/kg diet were formulated. Significantly higher (p < .05) weight gain (%), specific growth rate, with lower food conversion ratio were found in T320 and T340 groups. The protein utilizing efficiency and whole‐body protein content were significantly higher (p < .05) in the T320 group. Trypsin activity increased with the increasing dietary CP level but amylase activity decreased with the increasing dietary CP level. Transaminase enzymes, haemolymph protein and haemocyanin were elevated in T320 and T340 groups. The lactate dehydrogenase and malate dehydrogenase activities were significantly higher (p < .05) in the T240 group. Shrimp of T240 group had the lowest (p < .05) glycogen and total haemocyte count with highest (p < .05) haemolymph glucose and antioxidant enzymes activities than the other groups. Based on the results, feeding 320 g CP/kg is found to be optimum for supporting maximum growth and health status of L. vannamei reared in ISW at 8 g/L salinity. The finding of the present study will help in developing a low‐cost feed for L. vannamei reared in ISW. 相似文献
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Prasanta K. Subudhi Gerard B. Magpantay Ratna Karan 《Genetic Resources and Crop Evolution》2013,60(4):1263-1273
A genomic DNA fragment (pAA7-2) amplified by a random amplified polymorphic DNA primer from the rice cultivar IR68 was used to assess the genetic variation and genetic relationships among the species of genus Oryza. A Southern hybridization experiment of diverse upland rice accessions using a single restriction enzyme HindIII generated unique DNA fingerprint for each accession. The differential hybridization pattern reflecting the copy number variation of pAA7-2 in a collection of wild species and cultivated species of rice provided insight about the genetic relationships among them. All AA genome species exhibited clear banding pattern suggesting presence of fewer copies of this sequence. Strongest hybridization signal was obtained in species belonging to BB, CC, GG, BBCC, CCDD genomes, whereas weakest hybridization signal was visible in EE, FF, and HHJJ genome species. Oryza brachyantha was the most divergent species. Clear difference in banding pattern was evident between Oryza schlechteri and Oryza coarctata belonging to HHKK genome. Although pAA7-2 had no repetitive sequences often associated with hypervariable loci, homology to a putative unclassified expressed retrotransposon distributed over several rice chromosomes was responsible for the complex banding patterns. There were more sites homologous to pAA7-2 sequence in corn and sorghum genome compared with the rice genome. The study demonstrates the potential of pAA7-2 as a powerful molecular tool for DNA fingerprinting, genetic diversity, phylogenetic, and evolutionary studies in Oryza sativa and its wild relatives and other grasses. 相似文献
17.
Sheikh M. Masum Mohammad Amzad Hossain Hikaru Akamine Jun‐Ichi Sakagami Prasanta C. Bhowmik 《Weed Biology and Management》2016,16(3):119-131
A series of experiments was conducted in the laboratory and greenhouse of the Subtropical Field Science Center, University of the Ryukyus, Japan, from April to October 2015 to assess the allelopathic potential of 50 indigenous Bangladeshi rice varieties by using the donor–receiver bioassay, equal compartment agar method (ECAM), plant residue extract method and pot culture method. Lettuce (Lactuca sativa L.), cress (Lepidium sativum L.), radish (Raphanus sativus L.), barnyard grass (Echinochloa crus‐galli L. Beauv.) and jungle rice (Echinochloa colona L.) were used as the test plants. The highest inhibition effect was given by Boterswar, while the stimulating effect was given by Kartikbalam and Panbira in the donor–receiver bioassay and ECAM tests. Boterswar, Goria, Biron and Kartiksail were selected as the highest allelopathic‐potential varieties by the donor–receiver bioassay and ECAM. In the methanol extract test, Boterswar gave the strongest inhibitory effect on both barnyard grass and jungle rice, while Kartiksail gave the highest inhibitory effect on the jungle rice shoot. The growth parameters and total dry matter of barnyard grass in the greenhouse pot experiment were significantly reduced as a result of the application of aqueous extracts of the selected rice varieties, which was similar to the results of the laboratory experiments. The varieties of Boterswar, Goria, Biron and Kartiksail were selected as the most allelopathic among the 50 indigenous Bangladeshi rice varieties. These rice varieties could be used for the isolation and identification of allelochemicals and to further develop new varieties that are tolerant to weeds. 相似文献
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R Chinnaswamy H Lakshmi SS Kumari CM Anuradha CS Kumar 《Journal of insect science (Online)》2012,12(3):1-18
Abstract The activity of sericulture is declining due the reduction of mulberry production area in sericulture practicing countries lead to adverse effects on silkworm rearing and cocoon production. Screening for nutrigenetic traits in silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) is an essential prerequisite for better understanding and development of nutritionally efficient breeds/hybrids, which show less food consumption with higher efficiency conversion. The aim of this study was to identify nutritionally efficient polyvoltine silkworm strains using the germplasm breeds RMW(2), RMW(3), RMW(4), RMG(3), RMG(1), RMG(4), RMG(5), RMG(6) and APM(1) as the control. The 1(st) day of 5(th) stage silkworm larvae of polyvoltine strains were subjected to standard gravimetric analysis until spinning for three consecutive generations covering three different seasons on 19 nutrigenetic traits. Highly significant (p ≤ 0.001) differences were found among all nutrigenetic traits of polyvoltine silkworm strains in the experimental groups. The nutritionally efficient polvoltine silkworm strains were resulted by utilizing nutrition consumption index and efficiency of conversion of ingesta/cocoon traits as the index. Higher nutritional efficiency conversions were found in the polyvoltine silkworm strains on efficiency of conversion of ingesta to cocoon and shell than control. Comparatively smaller consumption index, respiration, metabolic rate with superior relative growth rate, and quantum of food ingesta and digesta requisite per gram of cocoon and shell were shown; the lowest amount was in new polyvoltine strains compared to the control. Furthermore, based on the overall nutrigenetic traits utilized as index or 'biomarkers', three polyvoltine silkworm strains (RMG(4), RMW(2), and RMW(3)) were identified as having the potential for nutrition efficiency conversion. The data from the present study advances our knowledge for the development of nutritionally efficient silkworm breeds/hybrids and their effective commercial utilization in the sericulture industry. 相似文献
19.
The fate of allelochemicals in the soil environment largely determines the expression of allelopathy in the natural environment. In allelopathy research, the sorption of allelochemicals onto soil particles has been less well studied than their degradation. A study was carried out to evaluate the growth of cucumber (Cucumis sativus var Marketmore 76) and radish (Raphanus sativus var Crimson giant) in soil amended with 1, 5, 10 and 20 mg l–1 benzoic acid as model allelopathic substance. Growth of both cucumber and radish was not inhibited in soil amended with benzoic acid. A labeled study indicates that sorption of benzoic acid onto soil particles increases with concentration. Benzoic acid isotherms of both soils were non-linear, with an N value of 0.875 for a garden soil and 0.891 for a garden soil + sand, and they may explain the reason for the limited allelopathic effect of benzoic acid at concentrations often recorded in natural soil. 相似文献
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Niladri Mukherjee Prasanta SainiSuprabhat Mukherjee Priya RoyPrajna Gayen Santi P. Sinha Babu 《Research in veterinary science》2014
Dirofilaria immitis is the causative agent of cardiopulmonary dirofilariasis in the Canine family. The aim of the study was to evaluate the efficacy of the ethanolic extract of Azadirachta indica leaves (EEA) against the microfilaria (mf) of D. immitis in vitro. EEA was evaluated for different compound classes through HPTLC. Relative motility, mortality and morphological alterations were observed in the mf after exposure to EEA. The effect of EEA on redox status in the treated mf was evaluated by some key enzymatic and non-enzymatic parameters. An enhanced reactive oxygen species (ROS) level in the treated mf along with altered redox status was evident. DNA fragmentation and terminal-deoxynucleotidyl-transferase dUTP nick end labeling (TUNEL) confirmed apoptosis. In addition, western blotting revealed down-regulation of anti-apoptotic protein and up-regulation of pro-apoptotic proteins. Taken together, the microfilaricidal activity of EEA can be attributed to its capacity to inflict oxidative stress culminating in apoptosis. 相似文献