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ABSTRACT: The macrolide class of antibiotics, including tylosin and tilmicosin, is widely used in the veterinary field for prophylaxis and treatment of mycoplasmosis. In vitro susceptibility testing of 50 strains of M. gallisepticum isolated in Israel during the period 1997-2010 revealed that acquired resistance to tylosin as well as to tilmicosin was present in 50% of them. Moreover, 72% (13/18) of the strains isolated from clinical samples since 2006 showed acquired resistance to enrofloxacin, tylosin and tilmicosin. Molecular typing of the field isolates, performed by gene-target sequencing (GTS), detected 13 molecular types (I-XIII). Type II was the predominant type prior to 2006 whereas type X, first detected in 2008, is currently prevalent. All ten type X strains were resistant to both fluoroquinolones and macrolides, suggesting selective pressure leading to clonal dissemination of resistance. However, this was not a unique event since resistant strains with other GTS molecular types were also found. Concurrently, the molecular basis for macrolide resistance in M. gallisepticum was identified. Our results revealed a clear-cut correlation between single point mutations A2058G or A2059G in domain V of the gene encoding 23S rRNA (rrnA, MGA_01) and acquired macrolide resistance in M. gallisepticum. Indeed, all isolates with MIC ≥ 0.63 μg/mL to tylosin and with MIC ≥ 1.25 μg/mL to tilmicosin possess one of these mutations, suggesting an essential role in decreased susceptibility of M. gallisepticum to 16-membered macrolides. 相似文献
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A canine distemper virus infection of badgers in a hunting range in Austria is described. A badger which was shot after showing symptoms of rabies infection and one which was found dead were examined by gross pathology and parasitological, histological, bacteriological and virological methods. The examination for rabies was negative in both cases. The badger which was found dead histologically showed signs of a non purulent panencephalitis, the shot animal showed hyperaemia and oedema of the brain. No cytoplasmatic or nuclear inclusion bodies could be observed. The aetiologic viral diagnosis was achieved by immunofluorescence. Using two canine distemper-specific conjugates a typical granular fluorescence of different strength could be observed in organ sections. The bacterial examination showed in both cases a secondary infection with opportunistic pathogenic bacteria (haem. E. coli and Pseudomonas aeruginosa). 相似文献