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51.
The effects of direct and microsomal activated aflatoxin B1 on chicken peritoneal macrophages in vitro. 总被引：1，自引：0，他引：1
Sephadex-elicited peritoneal exudate cells were cultured on glass coverslips in order to determine the effects of aflatoxin B1 (AFB1) on chicken macrophages. Adherent macrophage monolayers were exposed for 1 h to 5, 10, and 20 micrograms ml-1 of AFB1, directly or to 0.01, 0.1, 0.5, 1, and 5 micrograms ml-1 of AFB1 in the presence of a chicken microsomal mixed function oxidase system (MFO). After exposure, the macrophage cultures were washed and allowed to recover for 2 h in fresh culture medium. Parameters measured at 2 h post recovery period were the substrate adherence potential, morphological alterations, phagocytic ability, and number of sheep red blood cells (SRBC) internalized per phagocytic macrophage. Direct in vitro exposure to AFB1 resulted in a dose-dependent decrease in macrophage adherence potential, and an increase in cell damage as determined by nuclear disintegration and cytoplasmic blebbing, but no detrimental effects were observed on percent phagocytic cells or the number of internalized SRBC. However, significant reductions in adherence potential, increased morphological alterations, and reduced phagocytosis and internalization of SRBC were observed when MFOs were added to cultures treated with much lower doses of AFB1. Addition of piperonyl butoxide (a P-450 inhibitor) abrogated AFB1-MFO induced alterations. This study suggests that microsomal activated AFB1 causes significant alterations in chicken macrophage functions. 相似文献
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EM Stachowiak K Papis M Kruszewski T Iwane&#;ko T Bart&#;omiejczyk JA Modli&#;ski 《Reproduction in domestic animals》2009,44(4):653-658
It was suggested that the cryodamage to oocytes' DNA has been responsible for the compromised developmental competence of cryopreserved oocytes. Vitrification of bovine oocytes affected not only cellular components, but also nuclear material. A significant rate of DNA fragmentation was found in bovine frozen or vitrified oocytes analysed by Comet assay regardless of cryopreservation method. Our method of vitrification using droplet system after gentle pre-equilibration treatment is one of the most effective cryopreservation methods employed for bovine oocytes so far, making it possible to develop 30% blastocyst stage embryos. In this study, the extent of DNA damage in bovine oocytes vitrified using three vitrification methods (droplet system, Open Pulled Straw and traditional vitrification in 0.25 ml insemination straws) was compared using Comet assay. Vitrification in droplet system and Open Pull Straws vitrification did not result in detectable cryoinjuries of DNA of bovine oocytes. On the contrary, DNA fragmentation was found in four of 26 oocytes vitrified in 0.25 ml straws (15.4%, p ≤ 0.05 in comparison with the other vitrification methods). 相似文献
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CF Lúcio LCG Silva JA Rodrigues GAL Veiga CI Vannucchi 《Reproduction in domestic animals》2009,44(S2):208-210
There are limited data concerning blood gas parameters in neonatal dogs. Knowledge of the normal physiology may facilitate effective therapeutic intervention and potentially reduce neonatal mortality. This study examined acid–base parameters in pups born at normal parturition (n = 27) compared with those born after obstetrical assistance or caesarean operation (n = 13) and those born following oxytocin (OXY) administration for treatment of uterine inertia (n = 11). Pups were subjected to an objective scoring method of neonatal health adapted from use in humans (the Apgar score) at birth and again at 5 and 60 min after birth. Venous blood samples were collected at 5 and 60 min after birth for evaluation of blood gas parameters. At birth, all pups had low Apgar scores and a mixed acidosis. The base excess was lowest for pups delivered after OXY administration. The Apgar score improved for all pups after 5 min of birth and there was an improvement in carbon dioxide tension, base excess and venous blood pH at 1 h, although in all pups a metabolic acidosis persisted. These data provide an important insight into neonatal physiology and the variability of blood gas parameters in pups born at normal and abnormal parturition and provide the basis for clinical decision making following dystocia. 相似文献
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Ligation of the caudal mesenteric artery during resection and anastomosis of the colorectal junction for annular adenocarcinoma in two dogs 总被引：1，自引：1，他引：0
An 8-year-old terrier cross and a 10-year-old German Shorthaired Pointer presented to the University Veterinary Centre, Sydney, for investigation of long-standing tenesmus and dyschezia. Both patients had an annular adenocarcinoma at the colorectal junction. Exploratory laparotomy was performed and the affected large intestinal segment was removed by resection and anastomosis. In both dogs, the caudal mesenteric artery was intimately associated with the mass, necessitating its ligation and transection. Postoperatively, there was no evidence of anastomosis breakdown in either case and both animals recovered well from surgery. The dogs were euthanased 8 and 10 months, respectively, after surgery because of clinical signs relating to metastatic disease. 相似文献
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根据目前拖网渔船在节能方面的需要,采用导管螺旋桨以改善渔船的推进性能,并对导管螺旋桨的设计和具体应用的优的缺点作初步的探讨。 相似文献
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