Anti‐Mullerian Hormone Concentration and Antral Ovarian Follicle Population in Murrah Heifers Compared to Holstein and Gyr Kept Under the Same Management     

JM Baldrighi  MF Sá Filho  EOS Batista  RNVR Lopes  JA Visintin  PS Baruselli  MEOA Assumpção 《Reproduction in domestic animals》2014,49(6):1015-1020

This study was performed to evaluate plasma concentrations of anti‐Mullerian hormone (AMH) and the ovarian antral follicle population (AFP) in different genetic groups. Cyclic heifers (13 Bubalus bubalis [Murrah]; 15 Bos taurus [Holstein] and 10 Bos indicus [Gyr]) were maintained under the same management and were synchronized with two doses of 150 μg IM d‐cloprostenol administered 14 days apart. After the second d‐cloprostenol treatment, heifers had their ovaries scanned daily by ultrasound to define the day of ovulation. On the same day, the AFP was determined and a plasma sample was collected to measure AMH. Murrah heifers had less AFP (25.6 ± 2.1 follicles; p = 0.01) and plasma AMH concentration (0.18 ± 0.03 ng/ml; p < 0.001) than Gyr (60.0 ± 12.2 follicles and 0.60 ± 0.12 ng/ml of AMH); however, data were similar when compared to Holstein (35.9 ± 6.8 follicles and 0.24 ± 0.06 ng/ml of AMH) heifers. Regardless of genetic background, there was a positive relationship between the AFP and plasmatic AMH concentration (Murrah [r = 0.62; p < 0.01], Holstein [r = 0.66; p < 0.001] and Gyr [r = 0.88; p < 0.001]). Also, when heifers were classified according to high‐ or low‐AMH concentration based on the average within each genetic group, high‐AMH heifers had greater (p < 0.0001) AFP than low‐AMH heifers. In conclusion, both Murrah and Holstein heifers presented lower plasma AMH concentration and AFP when compared to Gyr.  相似文献   

Vesicular glutamate transporters 1 and 2 target to functionally distinct synaptic release sites     

Fremeau RT  Kam K  Qureshi T  Johnson J  Copenhagen DR  Storm-Mathisen J  Chaudhry FA  Nicoll RA  Edwards RH 《Science (New York, N.Y.)》2004,304(5678):1815-1819

Vesicular glutamate transporters (VGLUTs) 1 and 2 show a mutually exclusive distribution in the adult brain that suggests specialization for synapses with different properties of release. Consistent with this distribution, inactivation of the VGLUT1 gene silenced a subset of excitatory neurons in the adult. However, the same cell populations exhibited VGLUT1-independent transmission early in life. Developing hippocampal neurons transiently coexpressed VGLUT2 and VGLUT1 at distinct synaptic sites with different short-term plasticity. The loss of VGLUT1 also reduced the reserve pool of synaptic vesicles. Thus, VGLUT1 plays an unanticipated role in membrane trafficking at the nerve terminal.  相似文献   

Reconstruction of Current Flow and Imaging of Current-Limiting Defects in Polycrystalline Superconducting Films     

AE Pashitski  A Gurevich  AA Polyanskii  DC Larbalestier  A Goyal  ED Specht  DM Kroeger  JA DeLuca  JE Tkaczyk 《Science (New York, N.Y.)》1997,275(5298):367-369

Magneto-optical imaging was used to visualize the inhomogeneous penetration of magnetic flux into polycrystalline TlBa2Ca2Cu3Ox films with high critical current densities, to reconstruct the local two-dimensional supercurrent flow patterns and to correlate inhomogeneities in this flow with the local crystallographic misorientation. The films have almost perfect c-axis alignment and considerable local a- and b-axis texture because the grains tend to form colonies with only slightly misaligned a and b axes. Current flows freely over these low-angle grain boundaries but is strongly reduced at intermittent colony boundaries of high misorientation. The local (<10-micrometer scale) critical current density Jc varies widely, being up to 10 times as great as the transport Jc (scale of approximately 1 millimeter), which itself varies by a factor of about 5 in different sections of the film. The combined experiments show that the magnitude of the transport Jc is largely determined by a few high-angle boundaries.  相似文献   

Gene flow from transgenic wheat and barley under field conditions     

Keith T. Gatford  Zainuddin Basri  Jane Edlington  Julia Lloyd  Javed A. Qureshi  Richard Brettell  Geoffrey B. Fincher 《Euphytica》2006,151(3):383-391

In this study the frequency and distance of gene flow from transgenic wheat (Triticum aestivum) and barley (Hordeum vulgare) to non-transgenic wheat and barley crops was investigated under local field conditions. Trials were conducted in the Australian Capital Territory (ACT) and in South Australia (SA). Gene flow from transgenic wheat was confirmed at frequencies of 0.012% and 0.0037% in the ACT and SA, respectively. In both trials gene flow occurred over distances of less than 12 m. Gene flow was also detected from transgenic barley at a frequency of 0.005%, over a distance of less than 12 m. The results show that under Australian field conditions, gene flow occurs at extremely low frequencies and over very short distances. Physical separation of transgenic and non-transgenic cereal crops by greater than 12 m should ensure that contamination of adjacent non-transgenic cereal crops remains less than 0.02%, well below the level permitted under Australian regulations.  相似文献   

Genetic relationship in Vigna mungo (L.) Hepper and V. radiata (L.) R. Wilczek based on morphological traits and SDS-PAGE     

Abdul Ghafoor  Zahoor Ahmad  Afsari Sharif Qureshi  Muhammad Bashir 《Euphytica》2002,123(3):367-378

Thirty seven diverse genotypes of Vigna mungo and three of V. radiataresembling to V. mungo for seed characters were studied to determine the extent of genetic variation based on morphological characters. In addition, 4black seeded genotypes in each species and8 green seeded V. radiata genotypes were included for SDS-PAGE analysis. Seed proteins were analyzed through slab type SDS-PAGE. High variance was observed for plant height, days to flowering, days to maturity, number of branches/plant, number of pods/plant, pod length, seeds per pod,biomass yield/plant, grain yield/plant and harvest index (%) during 2 consecutive years. First four components of PCA with eigenvalues >1 contributed 78.7 and79.1% of the total variance amongst 40genotypes during 1998 and 1999. The populations with high PC₁ values were expected to be high yielding characterised by earlyness, high seed weight and harvest index, all traits strictly related to reproductive phase. Four clusters of genotypes were observed during both the years and genetic diversity was in association for both the years. Based on SDS-PAGE, specific bands were suggested to be used for identifying Vigna radiatafrom mixed germplasm with Vigna mungo. The SDS-PAGE proved to be a powerful tool for differentiating Vigna radiata and Vigna mungo, whereas a low level inter-specific genetic diversity was observed and no clear differentiation was observed both for agronomic characteristics and for geographical origin. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Pathologic fracture healing after femoral limb salvage in a dog     

MA Hayes  S Jemilo  P Muir  R Sullivan  JA Bleedorn 《Australian veterinary journal》2020,98(3):84-89

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Isolation and identification of novel tocotrienols from rice bran with hypocholesterolemic, antioxidant, and antitumor properties   总被引：6，自引：0，他引：6  

Qureshi AA  Mo H  Packer L  Peterson DM 《Journal of agricultural and food chemistry》2000,48(8):3130-3140

Two novel tocotrienols were isolated from stabilized and heated rice bran, apart from the known alpha-, beta-, gamma-, and delta-tocopherols and tocotrienols. These new tocotrienols were separated by HPLC, using a normal phase silica column. Their structures were determined by ultraviolet, infrared, nuclear magnetic resonance, circular dichroism, and high-resolution mass spectroscopies and established as desmethyl tocotrienol [3, 4-dihydro-2-methyl-2-(4,8,12-trimethyltrideca-3'(E),7'(E), 11'-trienyl)-2H-1-benzopyran-6-ol] and didesmethy tocotrienol [3, 4-dihydro-2-(4,8,12-trimethyltrideca-3'(E),7'(E), 11'-trienyl)-2H-1-benzopyran-6-ol]. These tocotrienols significantly lowered serum total and LDL cholesterol levels and inhibited HMG-CoA reductase activity in chickens. They had much greater in vitro antioxidant activities and greater suppression of B16 melanoma cell proliferation than alpha-tocopherol and known tocotrienols. Results indicated that the number and position of methyl substituents in tocotrienols affect their hypocholesterolemic, antioxidant, and antitumor properties.  相似文献   

Identification of toxigenic Pasteurella multocida in atrophic rhinitis of pigs by in vitro characterisation     

GJ EAMENS  PD KIRKLAND  MJ TURNER  JA GARDNER†  MP WHITE‡  CL HORNITZKY 《Australian veterinary journal》1988,65(4):120-123

Toxigenic strains of Pasteurella multocida were readily differentiated from non-toxigenic strains by an agarose overlay method using bovine turbinate cells or bovine lung cells. Cells which were young and densely confluent were best suited to this assay. The incubation period required to distinguish toxigenic strains was dependent on the confluence of the monolayers, which was affected by the seeding rate, cell passage level and growth time prior to overlay. The agarose overlay method correctly identified 11 of 11 reference strains of Pasteurella multocida, and visible cytotoxic changes were present in the monolayers after 48 to 65 h. Outbreaks of the enzootic form of atrophic rhinitis in 2 New South Wales piggeries were associated with the isolation of toxigenic type D strains of P. multocida.  相似文献   

Detection of Tritrichomonas foetus antigens in formalin-fixed, paraffin embedded sections by the peroxidase antiperoxidase technique     

CM CAMPERO  PW LADDS  RG HIRST  JA VAUGHAN  DE EMERY 《Australian veterinary journal》1989,66(8):264-266

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Use of hair root as a source of DNA for the detection of heterozygotes for recessive defects in cattle     

PJ HEALY  JA DENNIS  JF MOULE 《Australian veterinary journal》1995,72(10):392-392

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