Genetic manipulation for enhancing calcium content in potato tuber     

Park S  Kang TS  Kim CK  Han JS  Kim S  Smith RH  Pike LM  Hirschi KD 《Journal of agricultural and food chemistry》2005,53(14):5598-5603

Increased calcium (Ca) in potatoes may increase the production rate by enhancing tuber quality and storability. Additionally, increased Ca levels in important agricultural crops may help ameliorate the incidence of osteoporosis. However, the capacity to alter Ca levels in potato tubers through genetic manipulations has not been previously addressed. Here we demonstrate that potato tubers expressing the Arabidopsis H+/Ca2+ transporter sCAX1 (N-terminal autoinhibitory domain truncated version of CAtion eXchanger 1) contain up to 3-fold more Ca than wild-type tubers. The increased Ca appears to be distributed throughout the tuber. The sCAX1-expressing potatoes have normally undergone the tuber/plant/tuber cycle for three generations; the trait appeared stable through successive generations. The expression of sCAX1 does not appear to alter potato growth and development. Furthermore, increased Ca levels in sCAX1-expressing tubers do not appear to alter tuber morphology or yield. Given the preponderance of potato consumption worldwide, these transgenic plants may be a means of marginally increasing Ca intake levels in the population. To our knowledge, this study represents the first attempts to use biotechnology to increase the Ca content of potatoes.  相似文献   

Investigating landscape phase transitions in Mediterranean rangelands by recurrence analysis     

Giovanni Zurlini  Norbert Marwan  Teodoro Semeraro  K. Bruce Jones  Roberta Aretano  Maria Rita Pasimeni  Donatella Valente  Christian Mulder  Irene Petrosillo 《Landscape Ecology》2018,33(9):1617-1631

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Ultrasonographic and histopathological features in 8 cats with fibrotic small intestinal stricture     

Andrew Holloway  Mauro Pivetta  Roberta Rasotto 《Veterinary radiology & ultrasound》2019,60(4):423-431

Benign stricture is an uncommon cause of chronic small intestinal obstruction in the cat. The purpose of this retrospective case series was to describe the ultrasonographic features, histopathological findings, and clinical presentation in a group of cats with benign small intestinal stricture. Inclusion criteria were cats presenting during the period 2010‐2017, and that had ultrasonography and small intestinal stricture confirmed at surgery. For each cat, clinical data and ultrasonographic findings were retrieved from the medical record, and histopathology, where available, was reviewed. Eight cats met the inclusion criteria. The location of strictures was duodenum (1/8), mid‐ to distal jejunum (4/8), and ileum (3/8). Ultrasonographic findings included gastric distension (8/8) and generalized (3/8) or segmental (5/8) intestinal dilation consistent with mechanical obstruction. Ingesta did not propagate beyond the strictured segment. Wall thickening was mild to moderate (3‐6 mm). Normal wall layering was disrupted in all cats. Strictures were predominantly hypoechoic (7/8) and associated with hyperechoic peri‐intestinal mesentery (6/8). Annular strictures (5/8) were less than 15 mm in length whereas long‐segment strictures (3/8) were greater than 15 mm in length. Histopathology showed transmural disease with fibrosis and inflammation (8/8), often (6/8) extending into the bordering mesentery. The mucosa was the most severely affected layer and epithelial injury accompanied the mucosal fibrosis/inflammation. Clinical presentation reflected delayed diagnosis of chronic bowel obstruction with debilitation (8/8), marked weight loss (8/8), and prerenal azotemia (5/8). Benign fibrostenotic stricture should be considered a differential diagnosis in debilitated young cats presenting with chronic bowel disease and ultrasonographic features of intestinal obstruction.  相似文献   

Effects of concanavalin A on the progesterone production by bovine steroidogenic luteal cells in vitro          下载免费PDF全文

FC Destro  I Martin  FDC Landim‐Alvarenga  JCP Ferreira  JL Pate 《Reproduction in domestic animals》2016,51(5):848-852

The aim of this study was to evaluate the effects of concanavalin A (CONA) on the progesterone (P4) production by bovine steroidogenic luteal cells (LCs) in vitro. Luteal cells were collected during the mid‐luteal stage (at 10–12 days following ovulation) and processed in the laboratory. Luteal cells were grown for 7 days in a humid atmosphere with 5% CO₂, with or without 10% foetal bovine serum, and were subjected to the following treatments: control: no treatment; CONA (10 μg/ml); LH (100 μg/ml); CONA + LH; LH (100 μg/ml) + prostaglandin F2α (PGF2α) (10 ng/ml); CONA + LH + PGF2α. Samples of the culture media were collected on days 1 (D1) and 7 (D7) for P4 quantification. The cells were counted on D7 of culture. Differences between treatments were considered statistically significant at p < .05. Culture in the presence of CONA decreased the P4‐secreting capacity of LCs on D7 of culture, particularly in the absence of serum. The cell numbers did not change between treatments.  相似文献   

A card agglutination test for dog erythrocyte antigen 1 (DEA 1) blood typing in donor dogs: Determining an appropriate cutoff to detect positivity using a receiver operating characteristic curve     

Daniela Proverbio  Roberta Perego  Luciana Baggiani  Eva Spada 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2019,48(4):630-635

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Correction to: The synergistic effect of organic acids,phytochemicals and a permeabilizing complex reduces <Emphasis Type="Italic">Salmonella</Emphasis> Typhimurium 1,4,[5],12:i-shedding in pigs     

Jessica Ruggeri  Fabio Foresti  Roberta Pavesi  Alessia Terrini  Francesca Giudici  Diego Padoan  Attilio Corradi  Maria Cristina Ossiprandi  Paolo Pasquali  Giovanni Loris Alborali 《Veterinary research communications》2018,42(3):219-219

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Relationships among neuroscore,magnetic resonance imaging features,and intracranial pressure in sheep affected by slow‐growing brain lesions          下载免费PDF全文

Maria A. Evangelisti  Roberta Deiana  Valentino Melosu  Giovanni P. Burrai  Isabella Ballocco  Antonio Varcasia  Antonio Scala  Maria L. Manunta 《Veterinary radiology & ultrasound》2018,59(3):305-311

Diagnosing high intracranial pressure by clinical and diagnostic imaging is particularly challenging for chronic or slow‐growing lesions. The aim of this prospective case‐control study is to determine whether the neuroscore and brain magnetic resonance imaging (MRI) are related to the direct measurement of intracranial pressure in sheep affected by intracranial slow‐growing lesions due to chronic cerebral coenurosis (Coenurus cerebralis). Seventeen affected and 10 control sheep were included. All animals underwent a neurological examination, MRI of the brain, and direct measurement of intracranial pressure. The severity of clinical signs and MRI findings were scored. Data were statistically analyzed. The invasive intracranial pressure value was higher in affected animals. A severely altered neuroscore is related to an increased intracranial pressure beyond the normal threshold (P < 0.05). The volume of the calvarium was larger in affected animals than in control animals (P = 0.0001) and was positively influenced by the presence and volume of the parasitic cyst (r = 0.7881, P < 0.01). Several degrees of deviation and deformation of both the ventricular system and brain parenchyma were detected by MRI. Subjective MRI findings were not associated with intracranial hypertension. In conclusion, this study shows that in sheep affected by slow‐growing lesions, severe alterations in the neuroscore and the results of objective MRI are related to an increased intracranial pressure beyond the normal threshold.  相似文献   

A new lupane caffeoyl ester from Hippocratea volubilis     

Nelson Alvarenga  Esteban A. Ferro 《Fitoterapia》2000,71(6)

The extract of the rootbark of Hippocratea volubilis afforded a new triterpene caffeoyl ester, lupeol caffeate (1). Its structure was determined by means of spectroscopic evidence.  相似文献   

Damage by Homalinotus depressus in commercial coconut palm crops in the Amazonian region of Brazil     

Rafael Coelho Ribeiro  Walkymário de Paulo Lemos  Paulo Manoel Pontes Lins  Roberta de Melo Valente  Antonio Agostinho Müller  Alexandre Mehl Lunz  José Cola Zanuncio 《Phytoparasitica》2012,40(3):239-241

The coconut palm (Cocos nucifera L., Arecaceae) has great economic and social importance for many industrial and non-industrial products. Pests can reduce its productivity and cause its death. In 2005, larvae and adults of a Curculionidae were observed damaging the floral stalk of coconut palms in commercial plantations in the municipality of Moju, Pará State, in the Brazilian Amazon. Insects were identified as the black coconut bunch weevil, Homalinotus depressus (L.) (Coleoptera: Curculionidae: Cholini). This is the first report of this pest damaging coconut palms in Brazil.  相似文献   

Statistical characterization of sicilian olive oils from the Peloritana and Maghrebian zones according to the fatty acid profile   总被引：1，自引：0，他引：1  

Di Bella G  Maisano R  La Pera L  Lo Turco V  Salvo F  Dugo G 《Journal of agricultural and food chemistry》2007,55(16):6568-6574

This paper deals with the characterization of 475 Sicilian virgin olive oils (VOO) produced in 10 different crop years (from 1993 to 2004), according to the cultivar and the geographical origin by means of multivariate statistical analysis applied to fatty acids. In particular, the studied VOOs came from the Peloritana and Maghrebian geological zones. The fatty acid composition was determined by using the official gas chromatographic method. The results suggest that although the effect of the cultivar is significant in the olive oil classification based on the fatty acid composition, a predominant and well-defined geographic effect is also present. This study demonstrated that it is possible to employ an official and inexpensive analytical method coupled with the statistical analysis to ascertain the geographical origin and the cultivar of an extra virgin olive oil.  相似文献