The Dnmt3b splice variant is specifically expressed in in vitro-manipulated blastocysts and their derivative ES cells     

Horii T  Suetake I  Yanagisawa E  Morita S  Kimura M  Nagao Y  Imai H  Tajima S  Hatada I 《The Journal of reproduction and development》2011,57(5):579-585

Manipulation of preimplantation embryos in vitro, such as in vitro fertilization (IVF), in vitro culture (IVC), intracytoplasmic sperm injection (ICSI), somatic cell nuclear transfer (SCNT) and other assisted reproduction technologies (ART), has contributed to the development of infertility treatment and new animal reproduction methods. However, such embryos often exhibit abnormal DNA methylation patterns in imprinted genes and centromeric satellite repeats. These DNA methylation patterns are established and maintained by three DNA methyltransferases: Dnmt1, Dnmt3a and Dnmt3b. Dnmt3b is responsible for the creation of methylation patterns during the early stage of embryogenesis and consists of many alternative splice variants that affect methylation activity; nevertheless, the roles of these variants have not yet been identified. In this study, we found an alternatively spliced variant of Dnmt3b lacking exon 6 (Dnmt3bΔ6) that is specific to mouse IVC embryos. Dnmt3bΔ6 also showed prominent expression in embryonic stem (ES) cells derived from in vitro manipulated embryos. Interestingly, IVC blastocysts were hypomethylated in centromeric satellite repeat regions that could be susceptible to methylation by Dnmt3b. In vitro methylation activity assays showed that Dnmt3bΔ6 had lower activity than normal Dnmt3b. Our findings suggest that Dnmt3bΔ6 could induce a hypomethylation status especially in in vitro manipulated embryos.  相似文献   

Pathological studies on a case of bovine skin leukosis     

H M Okada  Y Chihaya  K Matsukawa  T Kurosawa  K Takahashi  M Sonoda 《Nippon juigaku zasshi. The Japanese journal of veterinary science》1987,49(3):411-418

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Effect of activation treatments on actin filament distribution and in vitro development of miniature pig somatic cell nuclear transfer embryos     

Yamanaka K  Sugimura S  Wakai T  Shoji T  Kobayashi J  Sasada H  Sato E 《The Journal of reproduction and development》2007,53(4):791-800

In the present study, we investigated the effect of activation treatments on the actin filament distribution and in vitro development of somatic cell nuclear transfer (SCNT) embryos in miniature pigs. We combined three activation methods, ionomycin (ION), electrical stimulation (ES), and cycloheximide treatment (CH), to prepare seven activation treatments (ION, ES, CH, ION + CH, ION + ES, ES + CH and ION + ES + CH). First, we investigated the activation rate of oocytes and in vitro development of parthenotes. The activation rates of the oocytes in the ION, ES, CH, ION + CH, ION + ES, ES + CH, and ION + ES + CH groups were 42.9, 51.3, 0.0, 82.1, 80.6, 78.1 and 78.6%, respectively, showing that the rates of the combined treatment groups were significantly higher (P<0.05) than those of the single treatment groups. Although there were no significant differences in the activation rates of the combined treatment groups, the developmental rate to blastocysts in the ION + CH treatment group (36.1%) was significantly higher (P<0.05) than the other combined treatment groups (14.6-24.7%). Subsequently, we investigated the in vitro development and distribution of microfilaments in SCNT embryos. The developmental rate to blastcysts of the SCNT embryos in the ION + CH treatment group (11.3%) was significantly higher (P<0.05) than in the ES and ION + ES + CH treatment groups (4.5 and 5.2%, respectively). The rate of normal actin filament distribution in the SCNT embryos activated with ION + CH was significantly higher (P<0.05) than those activated with ES or ION + ES + CH treatment (63.3 vs. 46.8 or 46.4%). In addition, the fragmentation rate of the SCNT embryos activated with ION + CH was significantly lower (P<0.05) than those activated with ION + ES + CH (14.9 vs. 26.1%). The present results suggest that an activation treatment of ionomycin combined with cycloheximide may avoid physical damage to microfilaments and result in improved subsequent development of miniature pig SCNT embryos.  相似文献   

Volatile chemicals identified in extracts from leaves of Japanese mugwort (Artemisia princeps pamp.)     

Umano K  Hagi Y  Nakahara K  Shoji A  Shibamoto T 《Journal of agricultural and food chemistry》2000,48(8):3463-3469

Extracts from leaves of Japanese mugwort (Artemisia princeps Pamp.) were obtained using two methods: steam distillation under reduced pressure followed by dichloromethane extraction (DRP) and simultaneous purging and extraction (SPSE). A total of 192 volatile chemicals were identified in the extracts obtained by both methods using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). They included 47 monoterpenoids (oxygenated monoterpenes), 26 aromatic compounds, 19 aliphatic esters, 18 aliphatic alcohols, 17 monoterpenes (hydrocarbon monoterpenes), 17 sesquiterpenes (hydrocarbon sesquiterpenes), 13 sesquiterpenoids (oxygenated sesquiterpenes), 12 aliphatic aldehydes, 8 aliphatic hydrocarbons, 7 aliphatic ketones, and 9 miscellaneous compounds. The major volatile constituents of the extract by DRP were borneol (10.27 ppm), alpha-thujone (3.49 ppm), artemisia alcohol (2.17 ppm), verbenone (1.85 ppm), yomogi alcohol (1.50 ppm), and germacren-4-ol (1.43 ppm). The major volatile constituents of the extract by SPSE were 1,8-cineole (8.12 ppm), artemisia acetate (4.22 ppm), alpha-thujone (3.20 ppm), beta-caryophyllene (2.39 ppm), bornyl acetate (2.05 ppm), borneol (1.80 ppm), and trans-beta-farnesene (1. 78 ppm).  相似文献   

Applicability of a combined staining method with fluorochromes for the visualization of microbial cells in a soil thin section     

Chisa Maeda  Ueru Tanaka  Masaki Sonoda  Takashi Kosaki 《Soil Science and Plant Nutrition》2013,59(3):745-750

Staining of microbial cells in a soil thin section provides useful information about the role of soil as an ecological niche by relating the in situ distribution of microorganisms to the state of pores under undisturbed conditions. For technical improvement, we developed a procedure consisting of a combined staining method with 5-sulfofluorescein diacetate (SFDA), a metabolic type fluorochrome, and magnesium salt of l-anilino-8-naphthalene sulfonic acid (Mg-ANS), an adsorption type fluorochrome, in order to detect and differentiate the living microbial cells from the dead ones in a single soil thin section. Using images under a fluorescence microscope, we also examined the applicability of SFDA for staining microbial cells embedded in soil thin sections, which had seldom been attempted. Through this study, we confirmed the following facts: i) combined staining with SFDA and Mg-ANS can be achieved without major modification of the general procedure for thin section preparation, ii) staining with SFDA can be applied to soil thin sections, and iii) detection of in situ distribution and differential identification of the living and dead microbial cells may be possible by the proposed combined staining method.  相似文献   

Application of the vibration method with additional mass to timber guardrail beams     

Yoshitaka Kubojima  Satomi Sonoda  Hideo Kato 《Journal of Wood Science》2018,64(6):767-775

This study examined whether or not weight, density, and Young’s modulus can be accurately measured by vibration test without weighing beams for timber guardrails. Bending vibration tests with and without the concentrated mass were performed on small clear round bars without the pith of spruce and cedar and on actual size round bars with the pith of cedar for the timber guardrail. The following results were obtained. The vibration method with additional mass could be applied to the round bar as well as the rectangular bar. It is possible that resonance frequency was decreased by the sawn split in the horizontal tapping. It is believed that the free ends condition is easier to realize than the fixed ends condition for cross beams for timber guardrails. The weight of the cross beam for the timber guardrail could be accurately estimated by the vibration method with additional mass under several testing conditions.  相似文献   

Genetic relationships between meat quality traits and fatty acid composition in Japanese black cattle          下载免费PDF全文

Keiichi Inoue  Noriaki Shoji  Takeshi Honda  Kenji Oyama 《Animal Science Journal》2017,88(1):11-18

Genetic parameters were estimated to investigate the relationships between meat quality traits and fatty acid composition from 11 855 Japanese Black cattle. The meat quality traits included beef marbling score (BMS), beef color score, firmness of beef (FIR), texture of beef (TEX) and beef fat color score (BFS). The data on fatty acid composition included oleic acid and monounsaturated fatty acids (MUFA) contents, the ratio of MUFA to saturated fatty acids (MUS) and the ratio of elongation. The heritability estimates for meat quality traits ranged from moderate (0.30) to high (0.72). The strong genetic correlations between them were useful for simultaneous genetic improvement. In addition, high heritability estimates of fatty acid composition, ranging from 0.60 to 0.63, indicated that they could also be improved genetically. The genetic correlations of fatty acid composition with BMS, FIR and TEX were weak and negative. In contrast, positive and stronger genetic correlations were found between BFS and fatty acid composition, in particular, related to the level of unsaturation (0.77 and 0.79 for MUFA and MUS, respectively). This implies that improving the level of unsaturation makes fat darker (more yellow) and thus requires balancing with BFS.  相似文献   

Diverse morphological and cytogenetic variation and differentiation of the two subspecies in <Emphasis Type="Italic">Aegilops geniculata</Emphasis> Roth,a wild relative of wheat     

Shoji Ohta 《Genetic Resources and Crop Evolution》2017,64(8):2009-2020

Aegilops geniculata Roth, a wild relative of wheat (2n = 4x = 28, genome UUMM), is distributed over the Mediterranean basin and nearby areas. The species consists of two subspecies, subsp. geniculata and subsp. gibberosa (Zhuk.) Hammer. The former is distributed over the whole species area and has been genetically analyzed, and the latter is endemic to Spain and North Africa and has not been genetically evaluated. In this study, to clarify the genetic variation and delineation of the two subspecies from a biosystematic viewpoint, morphological variation among 23 accessions of subsp. geniculata and three of subsp. gibberosa and chromosome pairing at meiosis and fertility in their intra- and inter-subspecific F₁ hybrids were examined. A principal component analysis based on the 11 spike characteristics clearly divided the 26 accessions into two groups representing the two subspecies. The inter-subspecific F₁ hybrids showed significantly lower frequencies of chromosome pairing, significantly higher frequencies of multivalents, and significantly lower fertilities relative to those of the intra-subspecific F₁ hybrids. It was concluded that wide-ranging cytogenetic variation is included in subsp. geniculata, that subsp. gibberosa, the intra-subspecific variation of which is small, is morphologically and cytogenetically differentiated from subsp. geniculata beyond the range of the intra-subspecific variation of subsp. geniculata, and that the two subspecies are effectively isolated reproductively by hybrid sterility. The results strongly suggested that western North Africa is one of the important diversity centers of Ae. geniculata, where two subspecies were differentiated in the past and grow together in the present.  相似文献   

Collagen content and architecture of the pectoralis muscle in male chicks and broilers reared under various nutritional conditions     

Chamali DAS  Bimol Chandra ROY  Ichiro OSHIMA  Hideyuki MIYACHI  Shotaro NISHIMURA  Hisao IWAMOTO  Shoji TABATA 《Animal Science Journal》2010,81(2):252-263

Varying chicken growth rates were induced with different nutritional regimes, and the collagen content and architecture of M. pectoralis (PT) were compared among 21‐day‐old chicks and broilers at 80 or 95 days of age. The percentage of muscle weight to live weight was higher in rapid growing chicks (8.4%) than slow growing chicks (6.3%). The 80‐day‐old broilers engaged in compensatory growth after the early slow growth period producing PT muscle at 11% of live weight. The 80‐ and 95‐day‐old chicks with restricted late growth after an early rapid growth period showed PT weight at 8% and 9% of live weight, respectively. Collagen content of the PT muscle markedly decreased from the chicks to the broilers. The collagen concentration was higher in the late‐growth restricted broilers (1.67–1.88 mg/g) than the compensatory growth broilers (1.01–1.10 mg/g). Collagen concentration did not differ between the rapid and slow growing chicks (2.72 and 2.94 mg/g). Scanning electron micrographs showed thick and thin perimysia, and honeycomb endomysia. In the perimysia, a stack layer of collagen platelets and a reticular layer of collagen fiber cords were distinguished and collagen baskets of adipocytes were observed. The perimysial collagen fibers became thicker during growth of the chicks to broilers. However, in the late‐growth restricted broilers, the perimysial collagen fibers seemed to have retarded development compared with the compensatory growth birds. The PT muscle of chickens develops optimally when body growth is enhanced. The PT muscle of the compensatory growth broilers had improved collagen architecture regardless of the marked decrease in collagen content.  相似文献   

Life in the fast lane: Revisiting the fast growth—High survival paradigm during the early life stages of fishes     

Dominique Robert  Jun Shoji  Pascal Sirois  Akinori Takasuka  Ignacio A. Catalán  Arild Folkvord  Stuart A. Ludsin  Myron A. Peck  Su Sponaugle  Patricia M. Ayón  Richard D. Brodeur  Emily Y. Campbell  Evan K. D'Alessandro  John F. Dower  Louis Fortier  Alberto G. García  Klaus B. Huebert  Marc Hufnagl  Shin-ichi Ito  Mikimasa Joh  Francis Juanes  Mitsuo Nyuji  Yoshioki Oozeki  Guido Plaza  Motomitsu Takahashi  Yosuke Tanaka  Naoki Tojo  Shingo Watari  Naotaka Yasue  Pierre Pepin 《Fish and Fisheries》2023,24(5):863-888

Early life survival is critical to successful replenishment of fish populations, and hypotheses developed under the Growth-Survival Paradigm (GSP) have guided investigations of controlling processes. The GSP postulates that recruitment depends on growth and mortality rates during early life stages, as well as their duration, after which the mortality declines substantially. The GSP predicts a shift in the frequency distribution of growth histories with age towards faster growth rates relative to the initial population because slow-growing individuals are subject to high mortality (via starvation and predation). However, mortality data compiled from 387 cases published in 153 studies (1971–2022) showed that the GSP was only supported in 56% of cases. Selection against slow growth occurred in two-thirds of field studies, leaving a non-negligible fraction of cases showing either an absence of or inverse growth-selective survival, suggesting the growth-survival relationship is more complex than currently considered within the GSP framework. Stochastic simulations allowed us to assess the influence of key intrinsic and extrinsic factors on the characteristics of surviving larvae and identify knowledge gaps on the drivers of variability in growth-selective survival. We suggest caution when interpreting patterns of growth selection because changes in variance and autocorrelation of individual growth rates among cohorts can invalidate fundamental GSP assumptions. We argue that breakthroughs in recruitment research require a comprehensive, population-specific characterization of the role of predation and intrinsic factors in driving variability in the distribution and autocorrelation of larval growth rates, and of the life stage corresponding to the endpoint of pre-recruited life.  相似文献