Endocrine disruption in a terrestrial isopod under exposure to bisphenol A and vinclozolin     

Marco F. L. Lemos  Cornelis A. M. van Gestel  Amadeu M. V. M. Soares 《Journal of Soils and Sediments》2009,9(5):492-500

Background, aim, and scope  

In the past decade there has been an increasing awareness about the possible consequences of human and wildlife exposure to endocrine disrupting compounds (EDCs). Bisphenol A (BPA) and vinclozolin (Vz) are EDCs which impacts on vertebrates have been largely investigated. Nevertheless, research on invertebrate effects, especially on soil organisms, are still largely under-represented. This work aims to extend the limited ecotoxicological datasets available and to provide tools to assess the effects of EDCs on the terrestrial species, using Porcellio scaber (Crustacea: Isopoda) as a model organism.  相似文献   

Ecotoxicological Assessment of Contaminated River Sites as a Proxy for the Water Framework Directive: an Acid Mine Drainage Case Study     

Tania Vidal  Joana Luísa Pereira  Nelson Abrantes  Amadeu M V M Soares  Fernando Gon?alves 《Water, air, and soil pollution》2012,223(9):6009-6023

Metal contamination of freshwater bodies resulting from mining activities or deactivated mines is a common problem worldwide such as in Portugal. Bra?al (galena ore) and Palhal (pyrrhotite, chalcopyrite, galena, sphalerite, and pyrite ore), located in a riverside position, are both examples of deactivated mining areas lacking implemented recovery plans since their shutdown in the early mid-1900s. In both mining areas, effluents still flow into two rivers. The purpose of this work was to evaluate the potential hazard posed by the mining effluents to freshwater communities. Therefore, short- and long-term ecotoxicological tests were performed on elutriates from river sediments collected at each site using standard test organisms that cover different functional levels (Vibrio fischeri, Pseudokirchneriella subcapitata, Lemna minor, and Daphnia sp.). The results show that elutriates from the sediments of Palhal were very toxic to all tested species, while in contrast, elutriates from Bra?al showed generally no toxicity for the tested species. Our study highlights the usefulness of using an ecotoxicological approach to help in the prioritization/scoring of the most critical areas impacted by deactivated mines. This ecotoxicological test battery can provide important information about the ecological status of each concerning site before investing in the application of time-consuming and costly methods defined by the Water Framework Directive or can stand as a meaningful complementary analysis.  相似文献   

Fatty acid profile of hair lambs and their crossbreds slaughtered at different weights     

Landim AV  Cardoso MT  Castanheira M  Fioravanti MC  Louvandini H  McManus C 《Tropical animal health and production》2011,43(8):1561-1566

This study aims to investigate the effect of breed and slaughter weight on fatty the acid profile of the Longissimus dorsi muscle in lambs. Twenty-four Santa Inês (SI), 24.5 Ile de France × 0.5 Santa Inês (Ile × SI) and 12.5 Texel × 0.5 Santa Inês (Te × SI), slaughtered at different weights (30, 35, 40, and 45 kg), were evaluated. The animals were reared with creep feeding to weaning and were feedlot finished, receiving a diet composed of 30% hay and 70% concentrate. The analyses of fatty acids were carried out on the Longissimus muscle of the 13th rib. The experiment was in a three by four factorial design. The total mean saturated fatty acid level was 44.88%, with 43.30% monounsaturated fats and 1.72% polyunsaturated. The major fatty acids found included oleic (43%), palmitic (22%), and stearic (18%). The concentration of desirable fatty acids varied from 61.56% to 66.78%, with Te × SI (66.78%) having the highest levels (P < 0.05). The slaughter weight affected (P < 0.05) the saturated and unsaturated (both mono and poly) fatty acid profiles. The 35-kg slaughter weight showed the most desirable fatty acid profile. The saturated myristic and stearic fatty acids decreased with an increase in the slaughter weight, but oleic acid increased. In the conditions of the present study, the meat from Santa Ines and Texel × Santa Ines lambs had fatty acid profiles more beneficial for human health due to the higher levels of unsaturated fatty acids.  相似文献   

Risk-based surveillance for avian influenza control along poultry market chains in South China: The value of social network analysis   总被引：1，自引：0，他引：1  

Martin V  Zhou X  Marshall E  Jia B  Fusheng G  FrancoDixon MA  DeHaan N  Pfeiffer DU  Soares Magalhães RJ  Gilbert M 《Preventive veterinary medicine》2011,102(3):196-205

Over the past two decades, the poultry sector in China went through a phase of tremendous growth as well as rapid intensification and concentration. Highly pathogenic avian influenza virus (HPAIV) subtype H5N1 was first detected in 1996 in Guangdong province, South China and started spreading throughout Asia in early 2004. Since then, control of the disease in China has relied heavily on wide-scale preventive vaccination combined with movement control, quarantine and stamping out. This strategy has been successful in drastically reducing the number of outbreaks during the past 5years. However, HPAIV H5N1 is still circulating and is regularly isolated in traditional live bird markets (LBMs) where viral infection can persist, which represent a public health hazard for people visiting them. The use of social network analysis in combination with epidemiological surveillance in South China has identified areas where the success of current strategies for HPAI control in the poultry production sector may benefit from better knowledge of poultry trading patterns and the LBM network configuration as well as their capacity for maintaining HPAIV H5N1 infection. We produced a set of LBM network maps and estimated the associated risk of HPAIV H5N1 within LBMs and along poultry market chains, providing new insights into how live poultry trade and infection are intertwined. More specifically, our study provides evidence that several biosecurity factors such as daily cage cleaning, daily cage disinfection or manure processing contribute to a reduction in HPAIV H5N1 presence in LBMs. Of significant importance is that the results of our study also show the association between social network indicators and the presence of HPAIV H5N1 in specific network configurations such as the one represented by the counties of origin of the birds traded in LBMs. This new information could be used to develop more targeted and effective control interventions.  相似文献   

Identification of Hammondia heydorni oocysts by a heminested-PCR (hnPCR-AP10) based on the H. heydorni RAPD fragment AP10     

Soares RM  Lopes EG  Keid LB  Sercundes MK  Martins J  Richtzenhain LJ 《Veterinary parasitology》2011,175(1-2):168-172

Toxoplasma gondii, Hammondia hammondi, Neospora caninum, Neospora hughesi and Hammondia heydorni are members of the Toxoplasmatinae sub-family. They are closely related coccidians with similarly sized oocysts. Molecular diagnostic techniques, especially those based on polymerase chain reaction (PCR), can be successfully applied for the differentiation of Hammondia-like oocysts. In this paper, we describe a rapid and simple method for the identification of H. heydorni oocysts among other members of the Toxoplasmatinae sub-family, using a heminested-PCR (hnPCR-AP10) based on a H. heydorni RAPD fragment available in molecular database. DNA of oocysts of H. heydorni yielded a specific fragment of 289-290 bp in the heminested-PCR assay. No product was yielded when the primers were used for the amplification of DNA extracted from T. gondii, N. caninum, N. hughesi and H. hammondi, thus allowing the differentiation of H. heydorni among other members of the Toxoplasmatinae sub-family. The hnPCR-AP10 was capable of detecting H. heydorni genetic sequences from suspensions with at least 10 oocysts. In conclusion, the hnPCR-AP10 proved to be a reliable method to be used in the identification of H. heydorni oocysts from feces of dogs.  相似文献   

Genotyping of potentially zoonotic Giardia duodenalis from exotic and wild animals kept in captivity in Brazil     

Soares RM  de Souza SL  Silveira LH  Funada MR  Richtzenhain LJ  Gennari SM 《Veterinary parasitology》2011,180(3-4):344-348

We have studied the variability of glutamate dehydrogenase (gdh) and small subunit ribosomal (SSU) rRNA coding genes of Giardia species in fecal samples isolated from wild and exotic animals in Brazil, and compared with homologous sequences of isolates from human and domestic animals characterized in previous studies. Cysts of Giardia duodenalis were obtained from feces of naturally infected monkeys (Alouatta fusca) (n=20), chinchillas (Chinchilla lanigera) (n=3), ostriches (Struthio camelus) (n=2) and jaguar (Panthera onca) (n=1). Assemblage AI was assigned to the unique isolate of jaguar. All the samples from monkeys, chinchillas, and ostriches were assigned to Assemblage B. There was little evolutionary divergence between the referred isolates and isolates described elsewhere. The Assemblage B isolates identified in this study were closely related to Assemblage BIV isolated from humans. The molecular identification of Assemblages A and B of G. duodenalis isolates from exotic and wild animals demonstrates that such hosts may be a potential reservoir for zoonotic transmission of G. duodenalis.  相似文献   

Combinations of cactus pear with different roughage sources on the production,chemical composition,and milk fatty acid profile of F1 Holstein/Zebu cows     

Soares  Camila  Rocha Júnior  Vicente Ribeiro  Monção  Flávio Pinto  Borges  Lucas Daniel Alcântara  Caldeira  Luciana Albuquerque  Costa  Natanael Mendes  Ruas  José Reinaldo Mendes  Rigueira  João Paulo Sampaio  da Cunha Siqueira Carvalho  Cinara  de Sales  Eleuza Clarete Junqueira  de Assis Pires  Daniel Ananias  Chamone  Julieta Maria Alencar 《Tropical animal health and production》2020,52(5):2567-2576

Tropical Animal Health and Production - The qualities of food, mainly of animal origin, have always been of concern to consumers. It is known that the composition of animals’ diets can...  相似文献   

YELLOW PASSION FRUIT PLANT NUTRITIONAL DIAGNOSIS AT DIFFERENT PHENOLOGICAL STAGES BY THE DIAGNOSIS AND RECOMMENDATION INTEGRATED SYSTEM METHOD     

Almy Junior Cordeiro de Carvalho  Patrícia Soares Furno Fontes  Marta Simone Mendonça Freitas  Pedro Henrique Monnerat  Alexandre Gomes Fontes 《Journal of plant nutrition》2013,36(4):614-626

This work was performed in the Rio de Janeiro State, Brazil, with the objective to evaluate the nutritional status of yellow passion fruit plants along different phenological stages, using the DRIS method. Fifty-four passion fruit cultivated areas with an annual yield productivity ranging from 6.95 to 33.8 t ha^?1 year^?1 and average productivity of 16.9 t ha^?1 year^?1 were selected in the region. The contents of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulfur (S), chlorine (Cl), iron (Fe), zinc (Zn), manganese (Mn), and boron (B) were evaluated. The reference standards were established (mean and variation coefficient) for the values of the nutrient concentration ratios, two by two, on samples from high yield productivity plantations and analyzed by the DRIS index of nutrients for the medium and low yield productivity areas. The established standards differed according with the phenological stage of the culture. In a general way, the mean content of the evaluated nutrients did not differed between the two productivity levels into each phonological stage. There was a difference for the Nutritional Limitation Order between different phenological stages of yellow passion fruit plants. The most negative DRIS indexes and the highest absolute values for the Average Nutritional Balance on yellow passion fruit plants in the region, were found for potassium in May, phosphorus in October and iron in January.  相似文献   

Genome-wide association study (GWAS) of Phaseolus vulgaris resistance to Xanthomonas citri pv. fuscans     

Ana Leticia R. Monteiro  Augusto S. L. Pantaleão  Jorge L. Badel  Pedro H. M. Soares  Vinicius Q. Carneiro  Pedro C. S. Carneiro  José Eustáquio S. Carneiro 《Plant pathology》2021,70(7):1733-1744

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Efficacy of Amblyseius largoensis (Muma) as a biocontrol agent of the red palm mite Raoiella indica (Acari: Tenuipalpidae)     

Lira  Vanessa de Araujo  Jumbo  Luis Oswaldo Viteri  de Freitas  Giselle Santos  Rêgo  Adriano Soares  Galvão  Andréia Serra  Teodoro  Adenir Vieira 《Phytoparasitica》2021,49(1):103-111

Phytoparasitica - The red palm mite, Raoiella indica Hirst (Acari: Tenuipalpidae), is a severe pest of coconut (Cocos nucifera L.), banana (Musa sp.), and some ornamental palms. The predatory mite...  相似文献