Immunogenic and antigenic properties of recombinant soluble glycoprotein of rabies virus   总被引：2，自引：0，他引：2  

Gupta PK  Sharma S  Walunj SS  Chaturvedi VK  Raut AA  Patial S  Rai A  Pandey KD  Saini M 《Veterinary microbiology》2005,108(3-4):207-214

Rabies virus glycoprotein is a type I transmembrane protein exposed on the surface on the mature virus particle that induces virus neutralizing antibodies. In the present study, 60 amino acid C-terminal hydrophobic anchor (transmembrane) and cytoplasmic domains of glycoprotein were deleted from full-length glycoprotein and fused with polyhistidine tag. The N-terminal viral signal peptide was also replaced with CD33 signal peptide for efficient secretion in mammalian cells. Following transfection of Madin Darby bovine kidney (MDBK) cells with plasmid encoding this soluble form of glycoprotein, polyclonal populations of stably transfected resistant cells were obtained after G418 selection. The protein was expressed as a glycosylated protein and secreted outside the cells utilizing N-terminal CD33 signal peptide. The secreted soluble glycoprotein was purified from cell culture supernatant by Ni--agarose affinity chromatography utilizing C-terminal polyhistidine tag. Like full-length glycoprotein, the expressed recombinant soluble glycoprotein was found to be immunogenic when injected in rabbits. In this study, we have assessed the potential of recombinant soluble glycoprotein as diagnostic antigen in ELISA and found that this recombinant protein can be used as diagnostic antigen in ELISA for detecting anti-glycoprotein antibodies in immunized host.  相似文献   

A questionnaire survey of the management and use of anthelmintics in cattle and antelope in mixed farming systems in Zimbabwe     

Madzingira O  Mukaratirwa S  Pandey VS  Dorny P 《Journal of the South African Veterinary Association》2002,73(2):70-73

A survey of the management of mixed farming of cattle and antelope and use of anthelmintics was conducted on eleven farms between August and December 1999 by a self-administered questionnaire. Seventeen antelope species ranging from grey duikers (Sylvicapra grimmia) to eland (Taurotragus oryx) occurred on the farms. Impala (Aepyceros melampus) was the most abundant antelope on the farms. Seventy-five per cent of the antelope species on the farms were grazers and mixed feeders and shared grazing with cattle. Most farmers (n = 8) did not consider the stocking density for cattle and antelope as an important management factor. Fifty-four per cent of the farmers (n = 6) routinely dewormed both cattle and antelopes. Albendazole and fenbendazole were the most commonly used drugs for deworming cattle (72.7%) and antelope species (54.5%). The deworming of antelope was carried out during the dry season, using albendazole-, fenbendazole-and rafoxanide-medicated supplementary feed blocks. Doramectin injections were given to antelopes on two farms. Cattle were dewormed preventively and according to the general body condition of the animal. Few farmers (n = 4) followed the recommended deworming programme for cattle in Zimbabwe and only one farmer followed a specified dosing programme for game. However, results from the survey on the deworming of game indicate that farmers perceived helminth infections in antelope to be important.  相似文献   

Management ofMeloidogyne incognita inArtemisia pallens with bio-organics     

Rakesh Pandey 《Phytoparasitica》2005,33(3):304-308

The sedentary endoparasitic root-knot nematode [RKN],Meloidogyne incognita (Kofoid and White) Chitwood, is worldwide an economically important agri-pest, reducing the yield and quality of crops. The traditional method of RKN control is based mainly on chemical nematicides, which will not be frequently available from 2005 onwards; their use is highly objectionable due to their major contribution to ground water contamination, and dangers to the environment and to human and animal health. Consequently, new and environmentally safe tools for RKN management are urgently needed. The objective of the present investigation was to evaluate different bio-organic wastes on RKN infestation and the growth / oil yield ofArtemisia pallens Wall. The tested wastes were: 1. Distillation waste ofMentha arvensis (menthol mint); 2.Murraya koengii (meethi neem / curry leaf); 3.Cymbopogon flexuosus (lemongrass); 4.C. martinii (palmarosa); 5.C. winterianus (citronella); 6.Pelargonium graveolens (geranium); 7.Pogostemon patchouli (patchouli); 8.Tagetes minuta (marigold); 9. Vermicompost ofM. arvensis, Chrysanthemum cinerariaefolium (pyrethrum),Tagetes minuta andC. winterianus; 10.Trichoderma harzianum isolate U; 11. VA fungiGlomus aggregatum; G. fasciculatum. The results obtained with these different bio-organics were compared with three controls: untreated — uninoculated, untreated — inoculated and the chemical pesticide carbofuran. Results revealed a considerable enhancement of herbage biomass, flower bud and oil yields when plants were treated with the distillation waste ofC. martinii, C. winterianus, M. koengii, M. arvensis, vermicompost ofC. cinerariaefolium, T. minuta, M. arvensis and bio-agentT. harzianum. Least nematode infections were recorded with the distillation waste ofM. koengii, C. martinii, C. flexuosus, and vermicompost ofT. minuta, C. cinerariaefolium andM. arvensis. The results revealed that these environmentally sound bio-organics could be used for replacement of chemical nematicides. http://www.phytoparasitica.org posting May 20, 2005. The work was conducted at CIMAP-CSIR, Lucknow, India.  相似文献   

Localisation of parasites in the stomach of horses of the region of Settat (Morocco)]     

H Ouhelli  J Cabaret  V S Pandey  A Elkhalfane 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1979,32(4):347-352

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Genomics,genetics and breeding of tropical legumes for better livelihoods of smallholder farmers     

Chris Ojiewo  Emmanuel Monyo  Haile Desmae  Ousmane Boukar  Clare Mukankusi‐Mugisha  Mahendar Thudi  Manish K. Pandey  Rachit K. Saxena  Pooran M. Gaur  Sushil K. Chaturvedi  Asnake Fikre  NPVR Ganga Rao  CV SameerKumar  Patrick Okori  Pasupuleti Janila  Jean Claude Rubyogo  Chigeza Godfree  Essegbemon Akpo  Lucky Omoigui  Stanley Nkalubo  Berhanu Fenta  Papias Binagwa  Michael Kilango  Magdalena Williams  Omari Mponda  David Okello  Mekasha Chichaybelu  Amos Miningou  Joseph Bationo  Dramane Sako  Sory Diallo  Candidus Echekwu  Muhammad Lawan Umar  Richard Oteng‐Frimpong  Haruna Mohammed  Rajeev K. Varshney 《Plant Breeding》2019,138(4):487-499

Legumes are important components of sustainable agricultural production, food, nutrition and income systems of developing countries. In spite of their importance, legume crop production is challenged by a number of biotic (diseases and pests) and abiotic stresses (heat, frost, drought and salinity), edaphic factors (associated with soil nutrient deficits) and policy issues (where less emphasis is put on legumes compared to priority starchy staples). Significant research and development work have been done in the past decade on important grain legumes through collaborative bilateral and multilateral projects as well as the CGIAR Research Program on Grain Legumes (CRP‐GL). Through these initiatives, genomic resources and genomic tools such as draft genome sequence, resequencing data, large‐scale genomewide markers, dense genetic maps, quantitative trait loci (QTLs) and diagnostic markers have been developed for further use in multiple genetic and breeding applications. Also, these mega‐initiatives facilitated release of a number of new varieties and also dissemination of on‐the‐shelf varieties to the farmers. More efforts are needed to enhance genetic gains by reducing the time required in cultivar development through integration of genomics‐assisted breeding approaches and rapid generation advancement.  相似文献   

Synergisms in Science: Climate Change and Integrated Pest Management Through the Lens of Communication—2019 Student Debates     

Jocelyn R Holt  Lina Bernaola  Kadie E Britt  Chirs McCullough  Morgan Roth  Jennie Wagner  Max Ragozzino  Leslie Aviles  Zhilin Li  Forest Huval  Manoj Pandey  Benjamin W Lee  Megan Asche  Abigail Hayes  Abigail Cohen  Adrian Marshall  Hannah E Quellhorst  Rachel V Wilkins  Valerie Nguyen  Jacqueline Maille  Rachel K Skinner  John J Ternest  Sarah Anderson  Scott W Gula  Kayleigh Hauri  Julius Eason  Megan Mulcahy  Scott Lee  James Michael Villegas  Patricia Shorter 《Journal of insect science (Online)》2020,20(5)

Every year, the Student Debates Subcommittee (SDS) of the Student Affairs Committee (SAC) for the annual Entomological Society of America (ESA) meeting organizes the Student Debates. This year, the SAC selected topics based on their synergistic effect or ability to ignite exponential positive change when addressed as a whole. For the 2019 Student Debates, the SAC SDS identified these topic areas for teams to debate and unbiased introduction speakers to address: 1) how to better communicate science to engage the public, particularly in the area of integrated pest management (IPM), 2) the influential impacts of climate change on agriculturally and medically relevant insect pests, and 3) sustainable agriculture techniques that promote the use of IPM to promote food security. Three unbiased introduction speakers gave a foundation for our audience to understand each debate topic, while each of six debate teams provided a strong case to support their stance or perspective on a topic. Debate teams submitted for a competitive spot for the annual ESA Student Debates and trained for the better part of a year to showcase their talents in presenting logical arguments for a particular topic. Both the debate teams and unbiased introduction speakers provided their insight toward a better understanding of the complexities of each topic and established a foundation to delve further into the topics of science advocacy and communication, climate change, and the many facets of integrated pest management.  相似文献   

Identification of quantitative trait loci associated with iron deficiency chlorosis resistance in groundnut (Arachis hypogaea)     

Santosh K. Pattanashetti  Manish K. Pandey  Gopalakrishna K. Naidu  Manish K. Vishwakarma  Omprakash Kumar Singh  Yaduru Shasidhar  Ishwar H. Boodi  Basavaraj D. Biradar  Roma Rani Das  Abhishek Rathore  Rajeev K. Varshney 《Plant Breeding》2020,139(4):790-803

Iron deficiency chlorosis is an important abiotic stress affecting groundnut production worldwide in calcareous and alkaline soils with a pH of 7.5–8.5. To identify genomic regions controlling iron deficiency chlorosis resistance in groundnut, the recombinant inbred line population from the cross TAG 24 × ICGV 86031 was evaluated for associated traits like visual chlorosis rating and SPAD chlorophyll meter reading across three crop growth stages for two consecutive years. Thirty-two QTLs were identified for visual chlorosis rating (3.9%–31.8% phenotypic variance explained [PVE]) and SPAD chlorophyll meter reading [3.8%–11% PVE] across three stages over 2 years. This is the first report of identification of QTLs for iron deficiency chlorosis resistance-associated traits in groundnut. Three major QTLs (>10% PVE) were identified at severe stage, while majority of other QTLs were having small effects. Interestingly, two major QTLs for visual chlorosis rating at 60 days (2013) and 90 days (2014) were located at same position on LG AhXIII. The identified QTLs/markers after validation across diverse genetic material could be used in genomics-assisted breeding.  相似文献   

Determination of Chemical Composition and Nutritive Value with Fatty Acid Compositions of African Mangosteen (<Emphasis Type="Italic">Garcinia Livingstonei</Emphasis>)     

Kadanthottu Sebastian Joseph  Sushma Bolla  Kavita Joshi  Medha Bhat  Krupa Naik  Sridevi Patil  Sreevani Bendre  Brunda Gangappa  Vidya Haibatti  Shivanand Payamalle  Smita Shinde  Yaser Hassan Dewir  Hosakatte Niranjana Murthy 《Erwerbs-Obstbau》2017,59(3):195-202

African mangosteen (Garcinia livingstonei T. Anderson) is native to Africa and is exotic to other parts of the world. It is a fruit bearing tree with multiple uses. This study was purposed to determine, by analysis, the chemical compositions and nutritive value of the fruits of African mangosteen. Proximate compositions varied with the portions such as epicarp, mesocarp, endocarp and seed tested. All fruit portions contained carbohydrate (37.67–95.02%) and crude protein (0.65–31.76%) as their major components. Moisture (0.45–3.42%), crude fat (1.23–19.55%), crude fiber (2.93–21.13%) and ash (1.76–5.44%) were also found at different levels, depending upon the portions. All portions of fruit were rich in macro and micro elements. The fruits also possessed phenolics (174.02–10.725?mg GAE per g), flavonoid (19.25 to 99.98?µg QE per g) and alkaloid (1.56 to 9.49?mg/kg) contents. The constitution of tannins and oxalate which contribute towards the anti-nutritive value was also low, thus making the fruits beneficial for consumption. Quality attributes like relative amount of oleic acid, total unsaturated fatty acid, acid value, free fatty acid, peroxide value and iodine value showed that the seed oil of African mangosteen can become one among the oleic acid based vegetable oils for consumption.  相似文献   

硼酸处理对Sensatiou芒果成熟的影响          下载免费PDF全文

曾燕如  SrivastavaGC  PandeyM  PrasadNK. 《浙江农林大学学报》1996,13(3):263-269

用10mmolL-1 硼酸处理Sensatinn芒果果实,日的在于了解硼酸时延缓成熟过程,尤其对延缓那些与成熟有关的生理变化是否有一定的作用。8d的成熟过程所观浏的结果表明.①硼酸处理的鲜果质量下降不如对照明显,果皮中叶绿素含量的变化也类似,②硼酸处理的果实中,果胶甲脂酶及多聚半乳糖醛酸酶的活性高于时照,且果胶甲酷酶的活性比多聚半乳糖醛酸酶的活性早2d达到峰值,⑧与对照相比较,硼酸能降低果实的呼吸作用,而时果肉的总糖含量没有影响。  相似文献   

Effects of organic acids on growth and phosphorus utilization in rainbow trout <Emphasis Type="Italic">Oncorhynchus mykiss</Emphasis>     

Abhed Pandey  Shuichi Satoh 《Fisheries Science》2008,74(4):867-874

ABSTRACT:   An experiment was conducted to investigate the effect of various organic acid (OA) supplementation on phosphorus (P) and nitrogen (N) retention by rainbow trout fed low fishmeal-based diets. Six experimental diets were formulated, and diet 0.5P was arranged as a positive control diet with 0.5% calcium phosphate and 0P as a negative control without additional P. Diets CA and LA were supplemented with 1% citric (CA), and lactic (LA) acids, respectively, and diets MHA and LTE were supplemented with 1% methionine hydroxy analog (MHA) and 1% liquid trace elements (LTE), respectively. Fish fed CA and LTE showed similar growth to that of the positive control diet. P retention was lowest in the 0.5P group and was elevated with CA and LTE diets. Therefore, in this study it is suggested that it might not be necessary to supplement the low fishmeal-based diet of rainbow trout with P if certain organic acids such as CA are added.  相似文献