排序方式： 共有19条查询结果，搜索用时 15 毫秒
11.
Direct intestinal absorption of red fruit anthocyanins, cyanidin-3-glucoside and cyanidin-3,5-diglucoside, into rats and humans. 总被引：7，自引：0，他引：7
T Miyazawa K Nakagawa M Kudo K Muraishi K Someya 《Journal of agricultural and food chemistry》1999,47(3):1083-1091
We determined red fruit anthocyanins, cyanidin-3-glucoside (Cy-g) and cyanidin-3,5-diglucoside (Cy-dg), incorporated into plasma and liver of rats and human plasma by UV-HPLC. Fifteen minutes after an oral supplementation of a mixture of 320 mg of Cy-g and 40 mg of Cy-dg/kg of body weight, rats showed an increase to a maximum of 1563 microg (3490 nmol) of Cy-g/L and 195 microg (320 nmol) of Cy-dg/L in plasma and 0.067 microg (0.15 nmol) of Cy-g/g and a trace of Cy-dg together with methylated metabolites such as peonidin-3-glucoside in liver. In human plasma, 30 min after intake (2.7 mg of Cy-g and 0.25 mg of Cy-dg/kg of body weight), an average of 11 microg (24 nmol) of Cy-g/L and a trace of Cy-dg were found. Cyanidin as aglycone of Cy-g and Cy-dg was not found in such plasma samples, neither were conjugated and methylated anthocyanins. The results indicated that anthocyanins are incorporated keeping structurally intact glycoside forms, from the digestive tract into the blood circulation system in mammals. 相似文献
12.
13.
Ozaki H Esaki H Takemoto K Ikeda A Nakatani Y Someya A Hirayama N Murase T 《Veterinary microbiology》2011,150(1-2):132-139
To investigate the effects of rearing practices of commercial broiler chickens on the incidence of antimicrobial resistance in commensal Escherichia coli isolates, fecal E. coli isolates obtained in 4 farms were screened for anitimicrobial resistance. Ten E. coli isolates were recovered from each of the fecal samples collected from 10 birds in the farms at the ages of 2 days, 14-17 days, and 47-50 days. In 2 out of the 4 farms, no antimicrobials were used during the rearing period. In the other two farms, following collection of the fecal samples at 14 and 15 days of age, oxytetracycline (OTC), sulfadimethoxine (SDMX), and tylosin were given to birds on one farm and SDMX was used in the other. Isolates resistant to ampicillin and OTC that were obtained from an untreated flock at different sampling times were closely related to each other by pulsed-field gel electrophoresis patterns (PFGE) of XbaI-digested chromosomal DNA. PFGE analysis together with in vitro conjugation experiments suggested that diversity of resistance phenotypes within a clone may be resulted from the acquisition and loss of R-plasmids in an untreated and a treated flock. The numbers of resistance phenotypes observed among fecal isolates increased during the growth of the chickens in all the farms. The results in the present study suggest that persistence of commensal E. coli strains resistant to antimicrobials even in the absence of antimicrobial administration. It is also hypothesized that horizontal transmission of resistance determinants resulted in the emergence of different resistance phenotypes in those farms. 相似文献
14.
Kubota K Someya Y Yoshida R Kobayashi A Morita T Koshino H 《Journal of agricultural and food chemistry》1999,47(2):685-689
(S)-(+)-O-methylmandelate esters of trans- and cis-1,3, 3-trimethyl-2-oxabicyclo[2.2.2]octan-5- and 6-ols (2- and 3-hydroxy-1,8-cineoles) were prepared, and eight diastereomers were separated. The absolute configuration of the asymmetric carbons of the cineole moiety of each diastereomer was determined by (1)H NMR data according to the Mosher theory. Each mandelate was reduced with LiAlH(4) to obtain optically pure hydroxy-1,8-cineoles, this being followed by acetylation to afford optically pure acetoxy-1, 8-cineoles. These acetates were subjected to chiral GC, using a cyclodextrin column, and the enantiomeric purity of trans- and cis-1, 3,3-trimethyl-2-oxabicyclo[2.2.2]octan-5- and 6-yl acetates in the aroma concentrate from the rhizomes of Alpinia galanga was determined as 93.9 (5S), 19.4 (5R), 63.5 (6R), and 100 (6R) % ee, respectively. The aroma character of each enantiomer was also evaluated by GC-sniffing. 相似文献
15.
Masuda T Yamada K Akiyama J Someya T Odaka Y Takeda Y Tori M Nakashima K Maekawa T Sone Y 《Journal of agricultural and food chemistry》2008,56(14):5947-5952
As a part of a research project on the elucidation of the chain-breaking antioxidation mechanism of natural phenols in food components, caffeic acid, a polyphenolic acid widely distributed in edible plants, was investigated. The identification and time course analysis of the antioxidation reaction products from methyl caffeate were carried out in the ethyl linoleate oxidation system. The antioxidation reaction produced a quinone derivative of methyl caffeate as an antioxidation product during the initial stage, which was identified by (13)C NMR. The quinone, however, was not the final product, and a further reaction occurred to produce several new peroxides. The isolation and structure determination of the peroxides revealed that they had tricyclic structures, which consisted of ethyl linoleate, methyl caffeate, and molecular oxygen. On the basis of the formation pathway of these products, an antioxidation reaction mechanism of methyl caffeate, including the redox reaction of the caffeate and Diels-Alder reaction of the produced peroxides, was proposed. 相似文献
16.
Nobutaka Someya Shinichi Numata Masami Nakajima Akira Hasebe Tadaaki Hibi Katsumi Akutsu 《Journal of General Plant Pathology》2003,69(4):276-282
The gene chiA, encoding for the endochitinase ChiA, was cloned from Serratia marcescens strain B2, a tomato epiphytic bacterium, and introduced into the epiphytic bacterium Erwinia ananas NR-1, isolated from rice phylloplane. The gene chiA was introduced under the control of two types of promoter into a broad-host-range plasmid vector. The vector contained various fragments with promoter activity isolated from E. ananas chromosomal DNA. The constructed vectors were designated pchiA-V1pcf9 and pchiA-V1pcf53 for their respective promoters. E. ananas NR-1 transformed with either of these vectors produced and secreted ChiA. The antifungal activity of ChiA produced by transformed E. ananas NR-1 was demonstrated in vitro by the inhibition of Pyricularia oryzae germ tube elongation such as bursting of the hyphal tip. Transformed E. ananas NR-1 suppressed the incidence of rice blast caused by P. oryzae under greenhouse conditions; however, the magnitude of the suppressive effect depended on which promoter was used. Both transformants and the nontransformant E. ananas NR-1 survived on rice phylloplane. It is expected that the rice epiphytic bacterium E. ananas NR-1 carrying a chitinolytic enzyme gene is an efficient biological control agent against rice blast. 相似文献
17.
Mitsuro Hyakumachi Hideki Takahashi Yoichi Matsubara Nobutaka Someya Masafumi Shimizu Kappei Kobayashi Masamichi Nishiguchi 《Journal of General Plant Pathology》2014,80(4):287-302
Microorganisms play an enormously important role in plant disease control. Research on biological control of plant pathogens has received major impetus and attracted many researchers during the past few decades due to the increased public concern on hazards associated with the use of synthetic pesticides. From research on utilizing specific antagonistic microorganisms, many effective biological control agents (BCAs) have been found and are increasingly implemented in integrated pest management strategies to control plant diseases. Here current research results on biological control against plant diseases carried out in Japan are reviewed by focusing on major categories of BCAs: fungi, bacteria and actinomycetes and attenuated viruses. 相似文献
18.
Shoshi Mizuta Yuuki Koizumi Shiori Inoue Chiaki Someya Masatomi Hosoi Yoshihiro Yokoyama Reiji Yoshinaka 《Fisheries Science》2013,79(5):833-839
A large size (400 kDa) non-collagenous protein was detected as a major component in the extract, with neutral salt solution, from the dermis of sea cucumber Apostichopus armata. On SDS-PAGE analysis, the 400 K component shifted to a lower molecular weight component (about 200 K) by reduction with 2-mercaptoethanol, and they were both reactive for periodic acid-Schiff (PAS) reaction staining. From these results, this protein was suggested to be a glycoprotein consisting of disulfide-bonded two subunits with almost equal molecular weight (200 K). In addition to relatively high contents (>100/1,000 residues) of aspartic and glutamic acids, cysteine was also detected (6.1/1,000 residues) in amino acid analyses of this protein partially purified by anion-exchange column chromatography. These combined results suggest the structural similarity of the 400 K component to fibronectins from other vertebrate and invertebrate animals. 相似文献
19.
Kujoth GC Hiona A Pugh TD Someya S Panzer K Wohlgemuth SE Hofer T Seo AY Sullivan R Jobling WA Morrow JD Van Remmen H Sedivy JM Yamasoba T Tanokura M Weindruch R Leeuwenburgh C Prolla TA 《Science (New York, N.Y.)》2005,309(5733):481-484
Mutations in mitochondrial DNA (mtDNA) accumulate in tissues of mammalian species and have been hypothesized to contribute to aging. We show that mice expressing a proofreading-deficient version of the mitochondrial DNA polymerase g (POLG) accumulate mtDNA mutations and display features of accelerated aging. Accumulation of mtDNA mutations was not associated with increased markers of oxidative stress or a defect in cellular proliferation, but was correlated with the induction of apoptotic markers, particularly in tissues characterized by rapid cellular turnover. The levels of apoptotic markers were also found to increase during aging in normal mice. Thus, accumulation of mtDNA mutations that promote apoptosis may be a central mechanism driving mammalian aging. 相似文献