Propagation of Asian isolates of canine distemper virus (CDV) in hamster cell lines     

Serageldeen Sultan  Nguyen Thi Lan  Toshiki Ueda  Ryoji Yamaguchi  Ken Maeda  Kazushige Kai 《Acta veterinaria Scandinavica》2009,51(1):38

Backgrounds

The aim of this study was to confirm the propagation of various canine distemper viruses (CDV) in hamster cell lines of HmLu and BHK, since only a little is known about the possibility of propagation of CDV in rodent cells irrespective of their epidemiological importance.

Methods

The growth of CDV in hamster cell lines was monitored by titration using Vero.dogSLAMtag (Vero-DST) cells that had been proven to be susceptible to almost all field isolates of CDV, with the preparations of cell-free and cell-associated virus from the cultures infected with recent Asian isolates of CDV (13 strains) and by observing the development of cytopathic effect (CPE) in infected cultures of hamster cell lines.

Results

Eleven of 13 strains grew in HmLu cells, and 12 of 13 strains grew in BHK cells with apparent CPE of cell fusion in the late stage of infection. Two strains and a strain of Asia 1 group could not grow in HmLu cells and BHK cells, respectively.

Conclusion

The present study demonstrates at the first time that hamster cell lines can propagate the majority of Asian field isolates of CDV. The usage of two hamster cell lines suggested to be useful to characterize the field isolates biologically.  相似文献   

Study of liquids absorption and retention capacities of new cellulosic materials and sodium cellulose carboxylmethylate prepared from Posidonia     

Khiari Ramzi  Ferchichi Ikhlass  M’henni Mohamed Farouk 《Fibers and Polymers》2010,11(4):593-597

The Posidonia is a sea plant which is abundant on the Tunisian coasts. Important quantities of Posidonia oceanica fragments are accumulated every year on the beaches which must be cleaned every summer. This plant contains an important ratio of cellulosic materials. On other hand, local sanitary articles industry use important quantities of absorbing materials. The aim of this work is to take advantage of this available biomass by using it as a raw material for the preparation of some of these absorbing materials. In this aim three cellulose qualities of various purities are prepared starting from Posidonia wastes and from these three cellulosic materials six qualities of sodium carboxymethylcellulose (CMCNa) are synthesised, these qualities differ by there substitution degree. The performances of all these materials are evaluated in term of absorption and retention capacities. With this end in view, three kinds of liquids are used: the deionized water, the synthetic urine and the salt solution. Then the evaluated performances are compared with those of some commercial absorbent materials used in local industries (industrial cellulosic pulp and superabsorbant). This comparison shows that the different prepared CMCNa have a retention capacity largely higher than those of the commercial cellulosic pulp. The difference can reaches 405 %. The evaluated absorption capacities of all the prepared CMCNa are generally similar to those of the commercial cellulosic pulp. However the tested superabsorbant presents, in all cases, the best performances in term of liquid absorption and retention.  相似文献   

Polyphenolics Profile, Antioxidant and Radical Scavenging Activity of Leaves and Stem of Raphanus sativus L.     

Syed Sultan Beevi  Mangamoori Lakshmi Narasu  Bandi Boje Gowda 《Plant foods for human nutrition (Dordrecht, Netherlands)》2010,65(1):8-17

Aerial parts (leaves and stem) of Raphanus sativus, which are usually discarded were found to possess potent antioxidant and radical scavenging activity, as measured by standard antioxidant assays. Methanolic and acetone extracts of R. sativus leaves had total polyphenolic content of 86.16 and 78.77 mg/g dry extract, which were comparable to the traditional rich sources such as green tea and black tea. HPLC identification of polyphenolics indicated the presence of catechin, protocatechuic acid, syringic acid, vanillic acid, ferulic acid, sinapic acid, o-coumaric acid, myricetin, and quercetin in leaves and stem. Among the different extraction solvents, methanolic extract of leaves and stem showed potent reductive capacity, significantly inhibited linoleic acid peroxidation and displayed metal chelating activity. Further, they scavenged free radicals effectively with IC₅₀ (half maximal inhibitory concentration) of 31 and 42 μg/ml for DPPH radical, 23 and 52 μg/ml for superoxide radical, 67 and 197 μg/ml for hydrogen peroxide, and 56 and 62 μg/ml for nitric oxide, respectively. Leaves showed most potent antioxidant and radical scavenging activity as compared to stem, which may be accounted for the high polyphenolic content. Leaves and stem of R. sativus, often under-utilized part of this vegetable, thus possessed considerable amount of polyphenolics. Hence, it should be regarded as a potential source of natural antioxidants and could be effectively employed as an ingredient in health or in functional food.  相似文献   

Acaricidal,repellent and oviposition deterrent activities of <Emphasis Type="Italic">Datura stramonium</Emphasis> L. against adult <Emphasis Type="Italic">Tetranychus urticae</Emphasis> (Koch)     

Nabi Alper Kumral  Sultan Çobanoğlu  Cem Yalcin 《Journal of pest science》2010,83(2):173-180

The ethanol extracts obtained from both leaf and seed in the Thorn apple (Datura stramonium L.) (Solanaceae) were investigated for acaricidal, repellent and oviposition deterrent properties against adult two-spotted spider mites (Tetranychus urticae Koch) (Acari: Tetranychidae) under laboratory conditions. Leaf and seed extracts, which were applied in 167,250 and 145,750 mg/l concentrations, respectively (using a Petri leaf disc-spray tower method), caused 98 and 25% mortality among spider mite adults after 48 h. The simple logistic regression analysis showed that the independent variable, an increase in the dose of leaf extract was associated with a significant increase in the death rate of T. urticae females, but an increase in the dosage of seed extracts did not have a significant effect. Using probit analysis and estimating the parameters with a confidence limit of 95%, we determined the LC₅₀ values of leaf extract to be 70,593 mg/l. According to Pearson’s χ² test, mites showed the strongest run off to bean leaf surfaces sprayed with both leaf and seed extracts (in sub-lethal doses: <7,500 mg/l and <25,000 mg/l, respectively) and moved towards surfaces that had not been sprayed with extracts. Furthermore, repeated measures ANOVA indicated a significant difference between the number of eggs laid on unsprayed bean leaves compared to bean leaves that were sprayed with leaf and seed extracts at sub-lethal doses, 2,500 and 25,000 mg/l concentrations, respectively. These results suggest that D. stramonium extracts could be used to manage the two-spotted spider mite.  相似文献   

Nutrient digestibility and feedlot performance of lambs fed diets varying protein and energy contents   总被引：1，自引：0，他引：1  

Sultan  Javed I.  Javaid  Asif  Aslam  Muhammad 《Tropical animal health and production》2010,42(5):941-946

Thirty-six Thalli male growing lambs were used in a completely randomized design with 2 × 2 factorial arrangement of treatments to evaluate the effect of varying levels of energy and protein on nutrient intake, digestibility, weight gain, and gain to feed ratio. Four experimental diets, i.e., low energy–low protein (LE-LP), low energy–high protein (LE-HP), high energy–low protein (HE-LP), and high energy–high protein (HE-HP) were formulated. The low- and high-energy diets contained 2.3 and 2.7 MJ/kg dry matter (DM) with 12% and 14% of crude protein. The lambs were fed ad libitum. Dietary energy and protein levels and their interactions influenced the nutrient intake. Maximum dry matter intake was noted in animals fed LE-HP diet followed by LE-LP, HE-HP, and HE-LP diets. Digestibility of DM and N increased (P < 0.01) and that of neutral detergent fiber decreased (P < 0.01) linearly with increasing levels of dietary energy and protein. Digestibility of N was only affected by protein level and interaction between energy and protein levels. Average daily gain was higher (P < 0.01) in lambs fed HE-HP diet followed by LE-HP, LE-LP, and HE-LP diets. Dietary energy and protein levels and their interaction had significant effect (P < 0.01) on gain to feed ratio.  相似文献   

Molecular characterization and marker based chemotaxonomic studies of Podophyllum hexandrum Royle     

Phalisteen Sultan  A.S. Shawl  Suriya Rehman  S. Fayaz Ahmed  P.W. Ramteke 《Fitoterapia》2010

Detailed chemical studies and RAPD analysis were done in different populations of Podophyllum hexandrum collected from high altitude regions of North Western Himalayas. Random amplified polymorphic DNA (RAPD) analysis revealed a high degree of genetic diversity among the 12 collected accessions, attributed to their geographical and climatic conditions. HPLC analysis also revealed variation in the concentration of two major marker compounds which lead to the identification of a chemotype. The study demonstrated that RAPD and chemical markers are very useful tools to compare the genetic relationship and pattern of variation among such prioritized and endangered medicinal plants.  相似文献   

Lodging-related morphological traits of hybrid rice in a tropical irrigated ecosystem   总被引：1，自引：0，他引：1  

M. Sirajul Islam  Shaobing Peng  Romeo M. Visperas  Nelzo Ereful  M. Sultan Uddin Bhuiya  A.W. Julfiquar 《Field Crops Research》2007

Hybrid rice (Oryza sativa L.) has been proven to be effective in increasing yield potential, but lodging often limits its yield in high-yielding environments. This study was conducted to determine genotypic variation in lodging resistance and lodging-related morphological traits among hybrid and non-hybrid check varieties, and to identify the key morphological traits associated with lodging resistance. Lodging behavior of 16 rice genotypes, including 12 hybrids and four inbreds, was studied at the International Rice Research Institute farm during the 2004 wet season (WS) and 2005 dry season (DS). Grain yield and visual score of lodging were determined at maturity. Lodging-related morphological traits were measured at 30 days after flowering. Large genotypic differences in lodging-related morphological traits were observed. Among these traits, dry weight per unit length, breaking resistance, and lodging index (bending moment/breaking resistance × 100) of lower internodes were significantly correlated with visual score of lodging. Several hybrids demonstrated high lodging resistance although their average plant height was over 120 cm. The lodging-related morphological traits and visual score of lodging in these hybrids were comparable with those of the non-hybrid check varieties with strong lodging resistance. These results suggest that increases in dry weight per unit length and breaking resistance of lower internodes are primary targets for reducing lodging index, thus improving overall lodging resistance of hybrid rice in a breeding program.  相似文献   

A scenario tree model for the Canadian Notifiable Avian Influenza Surveillance System and its application to estimation of probability of freedom and sample size determination     

Christensen J  Stryhn H  Vallières A  El Allaki F 《Preventive veterinary medicine》2011,99(2-4):161-175

In 2008, Canada designed and implemented the Canadian Notifiable Avian Influenza Surveillance System (CanNAISS) with six surveillance activities in a phased-in approach. CanNAISS was a surveillance system because it had more than one surveillance activity or component in 2008: passive surveillance; pre-slaughter surveillance; and voluntary enhanced notifiable avian influenza surveillance. Our objectives were to give a short overview of two active surveillance components in CanNAISS; describe the CanNAISS scenario tree model and its application to estimation of probability of populations being free of NAI virus infection and sample size determination. Our data from the pre-slaughter surveillance component included diagnostic test results from 6296 serum samples representing 601 commercial chicken and turkey farms collected from 25 August 2008 to 29 January 2009. In addition, we included data from a sub-population of farms with high biosecurity standards: 36,164 samples from 55 farms sampled repeatedly over the 24 months study period from January 2007 to December 2008. All submissions were negative for Notifiable Avian Influenza (NAI) virus infection. We developed the CanNAISS scenario tree model, so that it will estimate the surveillance component sensitivity and the probability of a population being free of NAI at the 0.01 farm-level and 0.3 within-farm-level prevalences. We propose that a general model, such as the CanNAISS scenario tree model, may have a broader application than more detailed models that require disease specific input parameters, such as relative risk estimates.  相似文献   

Equine pythiosis in Egypt: clinicopathological findings,detection, identification and genotyping of Pythium insidiosum     

Yasmine H. Tartor  Mohamed H. Hamad  Nasser Z. Abouzeid  Farouk A. El‐Belkemy 《Veterinary dermatology》2020,31(4):298-e73

  相似文献   

Genetic dissection of grain yield traits in a large collection of spring wheat (Triticum aestivum L.) germplasm     

Khan  Sultan Habibullah  Sajjad  Muhammad  Gulnaz  Saima  Waqar  Sundas  Shahzad  Munawar  Arain  Saima Mir 《Journal of Crop Science and Biotechnology》2022,25(2):215-223

Journal of Crop Science and Biotechnology - Understanding genetic architecture of a crop germplasm is necessary for designing a successful breeding program. Herein, we evaluated a large collection...  相似文献