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In this work, water-extraction of Vitis Vinifera L. (Black Grenache) leaves and analysis of aqueous extracts for anthocyanins were investigated. Vegetable leaves were cultivated in North of Tunisia at different dates. Anthocyanins were identified as main constituents in these natural dyeing materials using high performance liquid chromatography (HPCL). Dyeability of wool and its fastness properties using Vitis Vinifera L. leaves extract were also determined. Aqueous extract of Vitis Vinifera L. leaves has markedly yielded shades with good fastness properties. Thus, affordability is a point favorable for Vitis Vinifera L. leaves. Effects of dye bath pH and temperature on dyeability and fastness proprieties of wool fabrics by aqueous extract of Vitis Vinifera L. leaves were evaluated. Experimental results showed that fastness properties of dyed fabrics ranged from average to very good. Vitis Vinifera L. leaves extracts showed potential to dyeing wool fibers under highly acidic conditions (pH ≤2). The results obtained here revealed that natural dyes extracted from Vitis Vinifera L. leaves can be cost-effective for dyeing wool fabrics.  相似文献   
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本文从拟南芥中克隆到基因At1g30210的全长读码框。多序列比对结果表明,在推导的氨基酸水平上,该基因编码的蛋白含有特征性的TCP保守结构域,与已知的玉米、金鱼草和水稻的TCP家族基因中都存在显著性的保守性。另外通过酵母单杂交实验证明该基因编码的蛋白在酵母体内具有转录激活功能。同时RT-PCR实验结果表明,该基因在拟南芥花组织中表达量相对较高,具有明显的组织表达特异性。花器官高表达转录因子的克隆将为研究TCP基因调控植物花发育和花形态建成提供新的物质基础。  相似文献   
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A 10-month study of surface waters in Canadian Creek (Ballarat, Victoria, Australia) showed the significant influence of historic gold mining waste material. The investigation focussed on the hydrogeochemistry of the surface waters and soils in order to: (1) document the levels and seasonal trends in major, minor and trace elements in the creek, (2) identify the process by which As is released from the soil/waste mining material to surface waters. For most dissolved major and trace elements (Na, Ca, Mg, K, and As) in surface waters, the concentrations decreased with the increasing rainfall and flow conditions except for Al and Fe. Two sites selected along the creek (< 1 km apart) allowed evaluation of the possibility that mining waste material is contributing to the elevated As concentrations (up to 145 μg/l) in downstream surface water. Arsenic concentration varied more than 28 fold seasonally and was highest in autumn and lowest in spring. Elevated concentrations of As (up to 1946 mg/kg) at the downstream site indicated the presence of a source of As concentration in both surface and subsurface soils. Oxidation of arsenic sulphides under aerobic conditions with redox fluctuations (7 to 201 mV) could cause elevated As levels in the creek. Significant statistical correlations among the major cations (Ca, Na and Mg) point to a common source(s) resulting in neutral to slightly alkaline (pH ~ 6.5 to 7.8) surface water. Fe and Al secondary phases under oxidising conditions are a significant controlling mechanism for the mobilization of As in highly contaminated soils (> 1500 mg/kg) in the study area. The large As adsorption capacity of Fe and Al could be limiting extreme mobilization into the water. Rainfall with relatively low pH is possibly causing mobilisation of Al, Fe and As from highly alkaline soils (pH ≈ 9.0) into the nearby creek.  相似文献   
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A fast and reliable method for the determination of polycyclic aromatic hydrocarbons (PAHs) in water samples by solid-phase extraction (SPE) using centrifugation has been developed and optimized. A silica-based C18 cartridge was used; parameters affecting the extraction procedure such as type and volume of the elution solvent, breakthrough volume of the percolated water sample, drying of the sorbent, and evaporation of the elute have been studied. The innovation of this work was the examination of the use of a centrifugation technique in both the drying and elution steps. When combined with centrifugation, the volume of the elution solvent was reduced to 1 mL and the time for sorbent drying decreased also to 10 min under vacuum. Under optimal conditions, recoveries for the 16 U.S. EPA PAHs were between 70 and 85% and the relative standard deviation varied between 1 and 14%. Surrogate standard recoveries were similarly between 61 and 94% with a relative standard deviation between 2 and 15%. The simplicity of the described method, use of less of organic solvent, short procedure time, and good recoveries demonstrate the advantages of this environmentally friendly approach for routine analysis of numerous samples.  相似文献   
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ABSTRACT:   In an attempt to initiate seafood biotechnology in the Sultanate of Oman, Middle East, using underutilized fish, fish burgers from two different formulas were developed and the quality and storage stability were evaluated during storage at −20°C for 3 months. Quality and storage stability were evaluated through total aerobic and coliform bacterial count, peroxide value, protein solubility, and color. Total aerobic bacteria were reduced significantly ( P  < 0.05) by 84% and 97% of the initial load in formulas 1 and 2, respectively, whereas coliforms were completely destroyed in both formulas at the end of storage. Peroxide value increased ( P  < 0.05) but did not reach detectable levels of rancidity. Salt-soluble protein content decreased remarkably during the storage period. The L value showed good stability during storage, keeping products bright for 3 months. Fish burgers from both formulas were acceptable for 3 months at −20°C. Storage stability was rationalized by the effectiveness of freezing, and antimicrobial and antioxidant properties of food additives.  相似文献   
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Raphanus sativus, a common cruciferous vegetable has been attributed to possess a number of pharmacological and therapeutic properties. It has been used in indigenous system of medicine for the treatment of various human ailments in India. This present study evaluated the chemopreventive efficacy of different parts of R. sativus such as root, stem and leaves, extracted with solvents of varying polarity and investigated the molecular mechanism leading to growth arrest and apoptotic cell death in human cancer cell lines. Of the different parts, significant growth inhibitory effect was observed with hexane extract of R. sativus root. Analysis of hexane extract by GC-MS revealed the presence of several isothiocyanates (ITCs) such as 4-(methylthio)-3-butenyl isothiocyanate (MTBITC), 4-(methylthio)-3-butyl isothiocyanate (erucin), 4-methylpentyl isothiocyanate, 4-pentenyl isothiocyanate and sulforaphene. R. sativus root extract induced cell death both in p53 proficient and p53 deficient cell lines through induction of apoptotic signaling pathway regardless of the p53 status of cells. The molecular mechanisms underlying R. sativus-induced apoptosis may involve interactions among Bcl2 family genes, as evidenced by up-regulation of pro-apoptotic genes and down-regulation of anti-apoptotic genes along with activation of Caspase-3. Our findings present the first evidence that hexane extract of R. sativus root exerts potential chemopreventive efficacy and induces apoptosis in cancer cell lines through modulation of genes involved in apoptotic signaling pathway.  相似文献   
60.
Prevalence of bovine tuberculosis in Jigawa State,northwestern Nigeria   总被引:1,自引:1,他引:0  
We determined the prevalence of bovine tuberculosis in Jigawa State, northwestern Nigeria using the comparative intradermal tuberculin test in 22 herds consisting of 922 cattle with overall and individual prevalence of 1.08% (10/922) and 45.45% (10/22) obtained, respectively.  相似文献   
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