SSR-based genetic linkage analysis of resistance to crown rust (Puccinia coronata f. sp. lolii) in perennial ryegrass (Lolium perenne)   总被引：2，自引：0，他引：2  

J. L. Dumsday  K. F. Smith  J. W. Forster †  E. S. Jones ‡ 《Plant pathology》2003,52(5):628-637

Crown rust (caused by Puccinia coronata f. sp. lolii) is a serious foliar disease of the pasture and turfgrass perennial ryegrass (Lolium perenne). Previous genetic studies have detected both qualitative and quantitative resistance mechanisms, and interpretation of the genetic system is complicated by variation within the sexually reproducing pathogen. Resistant and susceptible parental genotypes of ryegrass were identified using a composite urediniospore population collected from three geographically distinct locations. A two-way pseudo-testcross mapping population was obtained as the F₁ progeny of the pair-cross between ryegrass parental genotypes Vedette₆ and Victorian₉. Both parents showed intermediate resistance against a pathogen population collected in a single geographical zone (Hamilton, Victoria), but in the F₁ population, significant variation for a range of resistance-associated characters was detected. Statistical analysis of phenotypic data suggested a major gene effect, hence bulked segregant analysis with map-assigned simple sequence repeat (SSR) markers was used to scan the genome. A marker showing strong association with resistance was assigned to linkage group (LG) 2 of perennial ryegrass. Analysis of 11 LG2 SSR markers defined an interval between loci xlpssrh03f03 and xlpssrk02e02 as containing the gene or genes (LpPc1) conferring crown rust resistance. Resistance gene determinants were inherited from both parents, with up to 80% of the total phenotypic variation explained by markers segregating from Vedette₆ and up to 26% of the variation explained by markers segregating from Victorian₉. The two contributions together resulted in an additive increase in effect, with fully resistant individuals requiring determinants from both parents. A conserved syntenic relationship was observed with linkage group B of Avena strigosa, which is the location of a cluster of resistance genes to the oat form of crown rust. The implications of this study for marker-assisted selection of disease resistance in perennial ryegrass are discussed.  相似文献   

Laboratory evaluation of the insect growth regulator lufenuron againstHelicoverpa armigera on cotton     

Butter  N. S.  Singh  Gurmeet  Dhawan  A. K. 《Phytoparasitica》2003,31(2):200-203

An insect growth regulator (IGR), lufenuron (Match 5EC), was tested for its toxicity toHelicoverpa armigera on cotton. Potency of the IGR against the larval stage of the pest was demonstrated with respect to larval instars; the LC₉₀ values of 1st, 2nd, 3rd, 4th and 5th instar larvae were 5.63, 7.89, 8.03, 11.39 and 14.76 mg a.i.l ⁻¹, respectively. However, different larval instars did not differ significantly with respect to LC₅₀ and LC₁₀. IGR-treated larvae had swollen heads and were significantly smaller (1.5–2.3 mm) than the untreated control (2.9 mm). Larval weight was significantly reduced from 190 mg in the control to 50–70 mg in the lufenuron treatment. IGR treatment in the larval stage significantly affected both pupal length and pupal weight. Pupal duration of the test insect was significantly extended by IGR treatment. Pupal deformities, including an inability to shed the last larval skin and formation of larval-pupal intermediates, occurred following treatment. A significant reduction in adult emergence was recorded. In addition, abnormalities in the form of development of cavities in the forewings of adult were evident. A significant decline in fecundity was noted in the studies. http://www.phytoparasitica.org posting Feb. 3, 2003.  相似文献   

Association of a Geminivirus with a Leaf Curl Disease of Sunn Hemp (Crotalaria juncea) in India     

S.K. Raj  R. Singh  S.K. Pandey  B.P. Singh 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(5):467-470

Natural occurrence of a geminivirus causing severe leaf curl disease on sunn hemp (Crotalaria juncea) was recorded in India. The association of a geminivirus with the disease was demonstrated by whitefly transmission tests and polymerase chain reaction (PCR) amplification of DNA fragments of expected sizes with three pairs of degenerate geminivirus primers. The PCR-amplified viral DNA fragments were further characterized by Southern hybridization with a geminivirus probe consisting of the cloned coat protein (CP) gene of Indian tomato leaf curl virus (ITLCV). Restriction fragment length polymorphism analysis of a PCR-amplified CP fragment revealed that the geminivirus from sunn hemp was different than ITLCV.  相似文献   

Breakdown of resistance in cotton to cotton leaf curl disease in Pakistan   总被引：5，自引：0，他引：5  

S. Mansoor  I. Amin  S. Iram  M. Hussain  Y. Zafar  K. A. Malik  R. W. Briddon 《Plant pathology》2003,52(6):784-784

  相似文献   

拮抗细菌菌株Bacillus spp.间的相互作用及其对水稻纹枯病的抑制   总被引：5，自引：0，他引：5       下载免费PDF全文

李湘民  胡白石  许志刚  Mew T. W. 《植物保护学报》2003,30(3):273-278

测定了6个Bacillus spp.和1个Pseudomonas sp.P-13(G-对照菌株)在PPM培养基上的相互作用。在总共42个组合中，有27个组合是可亲和性的。在以B5423为单剂之一的5个组合中，亲和性的组合有4个。在温室条件下通过分批播种，在水稻分蘖盛期喷以B5423-R(B5423的标记菌株，是利福平抗性的突变体)为单剂之一的4个亲和性组合的菌悬浮液，以检测该组合中B5423-R在水稻茎基部的种群数量。结果发现，与单剂B5423-R相比，在应用1～7天内，组合B5423-R B45中，B5423-R的群体数量显著性降低；组合B5423-R B95、B5423-R B77中，B5423-R的群体数量与单剂无显著差异；组合B5423-R B4313中，B5423-R的群体数量在应用1～4天内与单剂无显著性差异，但在应用第7天显著地高于单剂。离体叶片纹枯病痛斑面积的抑制试验表明，与各自的单剂相比，组合B95 B5423及B95 Er77显著地抑制了纹枯病病斑面积；而组合B45 B80、B45 B5423和B45 B95纹枯病病斑面积显著地高于各自的单剂；其余10个组合与各自的单剂无显著差异。  相似文献   

云南省马铃薯晚疫病菌的交配型、抗药性及生理小种分布的研究   总被引：17，自引：2，他引：17  

K.Y.Ryu  罗文富  杨艳丽  国立耘  郭华春  王毅  陈海如 《植物病理学报》2003,33(2):126-131

 从云南省陆良县、建水县和南涧县采集2000年秋播的马铃薯晚疫病标本,分离获得124个致病疫霉菌株,连同1999~2000年分离自昆明、曲靖等地的春播马铃薯晚疫病的10个菌株共134个,采用常规技术对这些菌株进行了交配型、抗药性及生理小种的研究。交配型测定结果为:陆良县的18个菌株中有3个为A₂交配型,其余15个菌株为A₁交配型,建水县和南涧县的106个菌株及昆明、曲靖1999~2000年的10个菌株都是A₁交配型。测定了83个菌株对瑞毒霉(metalaxyl)和烯酰吗啉(dimethomorph)的抗药性,结果有12.0%的菌株对瑞毒霉表现抗性,16.9%表现中抗,71.1%表现敏感,其中陆良、昆明和曲靖的抗瑞毒霉菌株的比例大于建水和南涧的抗药性菌株的比例;所测定的83个菌株都对烯酰吗啉表现敏感。生理小种鉴定结果表明,云南晚疫病菌为3.4、0、3和4生理小种,分别占鉴定菌株总数的48.0%、32.5%、15.6%和3.9%。但南涧仅发现3.4和0小种,而昆明有0、3、3.4和4小种,陆良有0、3和3.4小种,可见昆明和陆良地区的晚疫病菌生理小种比南涧地区的生理小种的组成更趋多样。  相似文献   

膨胀素在果实完熟中的作用   总被引：4，自引：0，他引：4  

马俊莲  张子德  Ann L.T.Powel  Alan B.Bennett 《园艺学报》2003,30(4):492-495

 概述了细胞壁蛋白质膨胀素(Expansin)的发现、作用机制、基因家族、功能和研究现状。重点论述了膨胀素在果实完熟中的作用及其与细胞壁酶之间的相互关系，提出了利用转基因技术调节果实完熟和软化的新策略。关键词：中图分类号：  相似文献   

Production and characterization of a monoclonal antibody specific to the M serotype of plum pox potyvirus     

D. Boscia  H. Zeramdini  M. Cambra  O. Potere  M.T. Gorris  A. Myrta  B. Di Terlizzi  V. Savino 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(5):477-480

A monoclonal antibody to an Albanian isolate of plum pox potyvirus (PPV) was obtained (MAbAL), that specifically recognized strain M of this virus. The specificity of MAbAL, assessed by comparative ELISA on 130 PPV isolates of different geographical origin, 22 of which were also tested by comparative IC-PCR, gave consistent and highly reproducible results. MAbAL seems to be elicited by a stable surface determinant that makes it particularly suitable for successful use under a wide range of conditions. MAbAL is an useful addition to the panel of PPV-specific MAbs available to date.  相似文献   

Non-structural plum pox potyvirus proteins detected by immunogold labelling     

Martin  M.T.  G&#;lie  B. 《European journal of plant pathology / European Foundation for Plant Pathology》1997,103(5):427-431

Plum pox potyvirus (PPV) induces in infected Nicotiana clevelandii cells characteristic crystalline inclusions known as nuclear inclusions (NI) when located in the nucleus and as dense material (Dm) when located in the cytoplasm. Crystalline inclusions contain protease (NIa) and RNA-dependent RNA polymerase (NIb) proteins. It is now well established for all potyviruses that cylindrical inclusions contain CI helicase ATPase protein (Martin et al., 1992). The intracellular location of other non-structural PPV proteins remains unknown. Using Escherichia coli expression vectors, specific antibodies were obtained against P1, P3, 6K2 and NIb PPV proteins for which antibodies were not yet available. As expected, NIb antiserum labelled crystalline inclusions. P1, P3 and 6K2 proteins were present in both types of crystalline inclusions found in the nucleus and in the cytoplasm of PPV-infected leaves of N. clevelandii, suggesting that nuclear inclusions and dense material were composed of the same proteins. This composition is discussed.  相似文献   

Herbicide options for weed control in papaya     

R.K. NISHIMOTO 《Integrated Pest Management Reviews》1997,2(3):109-111

This paper reviews the literature on weeds and weed control in papaya. There is limited research on this topic, and nearly all of the research focuses on herbicides. Effective use of paraquat and glyphosate in papaya is dependent on avoidance of spray contact to green bark and foliage. Pre-emergence herbicide tolerance is dependent on papaya age, size and maturity, and soil type. Only one herbicide, oryzalin is shown to be tolerated by papaya immediately after transplanting. Herbicides such as diuron and oxyfluorfen with a broader spectrum of weed control generally injure young papaya, however they can be effectively used if the initial application of these herbicides is delayed until papaya attains certain size or maturity indices. There is a need for further research on weeds and weed control to improve the efficiency of papaya production  相似文献