Distribution of three algicidal <Emphasis Type="Italic">Alteromonas</Emphasis> sp. strains in seagrass beds and surrounding areas in the Seto Inland Sea,Japan     

Tomoko Sakami  Setsuko Sakamoto  Shuzo Takagi  Nobuharu Inaba  Ichiro Imai 《Fisheries Science》2017,83(1):113-121

Harmful algal blooms (HABs) often cause great damage to the fish aquaculture industry in the western part of Japan. Bacteria that effectively kill such phytoplankton are found in coastal seawater and are considered to influence the occurrence of HABs. Algicidal bacteria are found in abundance in seagrass beds; however, the distribution of particular bacterial strains has not yet been clarified. In this study, we determined the abundance of three algicidal Alteromonas sp. strains that were isolated as Chattonella antiqua-killing bacteria in seagrass beds and their surrounding areas in summers of 2013–2015. The strains were detected using a quantitative polymerase chain reaction (qPCR) amplification method. Two of the three algicidal bacterial strains were remarkably abundant in the seawater of an enclosed seagrass bed compared to the surrounding areas. In addition, the abundance of the three algicidal bacteria decreased when the eelgrass withered in late summer. These results suggest that growth of these algicidal bacteria was stimulated by eelgrass. The bacterial abundance estimated by qPCR was much greater than that determined by the culture-dependent method in June 2015 when the eelgrass grew thickly. This implies that the qPCR assay could be a sensitive tool to evaluate algicidal bacteria in natural environments.  相似文献   

Rapid and convenient method for preparing aurapten-enriched product from hassaku peel oil: implications for cancer-preventive food additives     

Takahashi Y  Inaba N  Kuwahara S  Kuki W  Yamane K  Murakami A 《Journal of agricultural and food chemistry》2002,50(11):3193-3196

Aurapten (7-geranyloxycoumarin) has been reported to be an effective inhibitor of chemical carcinogenesis in some rodent models. In the present study, a method for preparing an aurapten-enriched agricultural product has been established. Out of 17 Rutaceae varieties, the aurapten content in hassaku (Citrus hassaku Hort ex Y. Tanaka) fruit peel was marked, as well as that in natsumikan (C. natsudaidai) and grapefruit (C. paradisi). The aurapten content in hassaku peel was most abundant in April. Hassaku fruit peel oil, which was dissolved by heating precipitates including aurapten which had formed after freezing the peel oil at -20 degrees C, was used. After adsorbing aurapten from peel oil onto synthetic adsorbent SP70, the adsorbent was washed with 40% (v/v) ethanol in water to remove essential oils and pigments remaining on the adsorbent. Aurapten was then eluted with 80% (v/v) ethanol. In a laboratory-scale test, the recovery rates of aurapten and total carotenoids from the eluates were 74.3 and 4.6%, respectively. In a pilot-scale test, the recovery rate of aurapten in the aurapten-enriched preparation from dissolved hassaku oil was 91.0%, and its concentration was 64.1% (w/w). When stored for 180 days under sunlight, aurapten in powder form remained at 88.0-89.0% of the initial level, but only 31.3-43.8% in ethanol. The stability of aurapten in the aurapten-enriched preparation was higher than that of purified aurapten. These results suggest that aurapten is readily recovered from hassaku peel oil using SP70, and thus may be used as a food additive.  相似文献   

Parasitological survey on wild carnivora in north-western Tohoku, Japan.     

H Sato  T Inaba  Y Ihama  H Kamiya 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1999,61(9):1023-1026

In the winter of 1997-1998, we collected parasitological data from 60 wild carnivora in the north-western part of Tohoku region, Japan. These included 7 foxes (Vulpes vulpes japonica), 20 raccoon dogs (Nyctereutes procyonoides viverrinus), 29 martens (Martes melampus melampus), 3 weasels (two Mustela sibirica itatsi and one M. nivalis namiyei), and one Japanese badger (Meles meles anakuma). Roundworms (Toxocara canis in foxes and Toxocara tanuki in raccoon dogs), hookworms (Ancylostoma kusimaense and Arthrostoma miyazakiense) and Molineus sp. in the small intestine were the most prevalent in foxes and raccoon dogs. In martens, Aonchotheca putorii in the stomach, Concinnum ten in the pancreatic duct, Molineus sp. and Euryhelmis costaricensis in the small intestine were the most prevalent. Collected parasites include some new helminth species for this region or Japan; the strobilar stage of Taenia polyacantha from foxes, Pygidliopsis summa from a raccoon dog, Eucoleus aerophilus, A. putorii, and Soholiphyme baturini from martens.  相似文献   

Inhibitory effects of CIDR-based ovulation-synchronization protocols on uterine PGF2alpha secretion at the following luteal phase in early postpartum non-cycling beef cows     

Sakase M  Kawate N  Nakagawa C  Fukushima M  Noda M  Takeda K  Ueno S  Inaba T  Kida K  Tamada H  Sawada T 《The Journal of reproduction and development》2006,52(4):497-502

We investigated whether CIDR-based ovulation-synchronization protocols inhibit secretion of prostaglandin (PG) F2alpha from the uterus in the following luteal phase in non-cycling beef cows. Ten early (a month) postpartum non-cycling Japanese Black beef cows were treated with (1) Ovsynch (GnRH analogue on Day 0, PGF2alpha analogue on Day 7, and GnRH analogue on Day 9; n=3), (2) Ovsynch+CIDR (Ovsynch protocol plus a CIDR for 7 days from Day 0; n=4), or (3) estradiol benzoate (EB) Ovsynch+CIDR (EB on Day 0 in lieu of the first GnRH treatment followed by the Ovsynch+CIDR protocol; n=3). An oxytocin challenge was administered on Day 24 to examine uterine PGF2alpha secretion. Plasma concentrations of 13,14-dihydro-15-keto- PGF2alpha were lower at 30-120 min after oxytocin administration in the Ovsynch+CIDR group and 75 min after administration in the EB Ovsynch+CIDR group than in the Ovsynch group (P<0.05). Plasma progesterone concentrations were higher from Days 1 to 7 in the Ovsynch+CIDR group and from Days 1 to 5 in the EB Ovsynch+CIDR group than in the Ovsynch group (P<0.05). The progesterone concentrations were higher on Days 27 and 29 in both CIDR-treated groups than in the Ovsynch group (P<0.05). In conclusion, in non-cycling beef cows, CIDR-based ovulation-synchronization protocols inhibit uterine PGF2alpha secretion in the following luteal phase and prevent premature luteolysis as is seen with the Ovsynch protocol.  相似文献   

Azospirillum brasilense PII proteins GlnB and GlnZ do not form heterotrimers and GlnB shows a unique trimeric uridylylation pattern     

Juliana Inaba  Luciano F. Huergo  Ana C. Bonatto  Leda S. Chubatsu  Rose A. Monteiro  M. Berenice Steffens  Giseli Klassen  Liu U. Rigo  Fábio O. Pedrosa  Emanuel M. Souza 《European Journal of Soil Biology》2009,45(1):94-99

In many organisms, nitrogen metabolism is co-ordinated by a class of highly conserved proteins from the P_II family. In Gram-negative bacteria P_II proteins are trimers that can be covalently modified by uridylylation according to the cellular nitrogen status. Several prokaryotes have more than one gene that code for P_II proteins. In Escherichia coli it was shown that the two P_II proteins (GlnB and GlnK) can form heterotrimers and it was suggested that heterotrimerization of P_II proteins could be widespread in Bacteria. The nitrogen-fixing plant-associative bacteria Azospirillum brasilense code for two P_II proteins, GlnB and GlnZ. The expression of glnB and glnZ genes are induced under nitrogen fixing conditions and these proteins control both the expression and the activity of the nitrogenase enzyme. Here we show that unlike E. coli P_II proteins, A. brasilense GlnB and GlnZ, do not form heterotrimers in vitro. Our data also suggest that A. brasilense GlnB shows a unique uridylylation pattern.  相似文献   

Cytoskeletal and mitochondrial properties of bovine oocytes obtained by Ovum Pick‐Up: the effects of follicle stimulation and in vitro maturation          下载免费PDF全文

Tamás Somfai  Satoko Matoba  Yasushi Inaba  Michiko Nakai  Kei Imai  Takashi Nagai  Masaya Geshi 《Animal Science Journal》2015,86(12):970-980

Follicle stimulation by follicular stimulating hormone (FSH) is known to improve developmental competence of bovine oocytes obtained by Ovum Pick‐Up (OPU); however, the exact factors in oocytes affected by this treatment have remained unclear. We compared in vitro matured (IVM) oocytes obtained at the immature stage from cows by OPU either without or with stimulation with FSH (non‐stimulated and stimulated OPU, respectively) to those obtained by superstimulation and in vivo maturation in terms of cytoskeleton morphology, mitochondrial distribution, intracellular adenosine triphosphate (ATP) content and H₂O₂ levels at the metaphase‐II stage and intracellular Ca²⁺ levels after in vitro fertilization (IVF). Confocal microscopy after immunostaining revealed reduced size of the meiotic spindle, associated with increased tendencies of microfilament degradation and insufficient mitochondrial re‐distribution in non‐stimulated OPU‐derived IVM oocytes compared with those collected by stimulated OPU, which in turn resembled in vivo matured oocytes. However, there was no difference in mitochondrial functions between oocytes obtained by stimulated or non‐stimulated OPU in terms of ATP content, cytoplasmic H₂O₂ levels, base Ca²⁺ levels and the frequencies and amplitudes of Ca²⁺ oscillations after IVF. Larger size of metaphase spindles in oocytes obtained by stimulated OPU may reflect and potentially contribute to their high developmental competence.  相似文献   

Physicochemical properties of pseudorabies virus hemagglutinin.     

J Kataoka  Y Inaba  N Tetsu  I Shibata  K Yoshiki  T Hirahara  A Izumida 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》1991,53(6):981-987

Pseudorabies virus hemagglutinin was readily adsorbed on mouse erythrocytes at 4, 22, or 37 degrees C, but not on cattle erythrocytes. The adsorbed hemagglutinin could not be eluted from the cells by resuspending in phosphate-buffered saline (PBS), by incubating at 37 or 50 degrees C, or by incubating in the presence of neuraminidase. The receptor on mouse erythrocytes for the hemagglutinin was inactivated by trypsin, but not by neuraminidase, sodium deoxycholate (DOC), potassium periodate (KIO4), dithiothreitol (DTT), 2-mercaptoethanol (2-ME) and formalin. The hemagglutinin was inactivated by trypsin, alpha-amylase, pepsin, DOC, KIO4, and ethylendiamine-tetraacetic acid (EDTA), but not by papain, beta-glucosidase, phospholipase C, neuraminidase, DTT, 2-ME, Tween-80, ethylether, chloroform, trichloro-trifluoroethane, beta-propiolactone and formalin, suggesting that the hemagglutinin active component involved glycoproteins. The hemagglutinin was stable at 37 degrees C for lower temperatures but not at 60 degrees C or higher. The hemagglutinin activity was resistant to ultraviolet irradiation, while the infectivity was very susceptible. The hemagglutinin and the infectivity were readily sedimented by ultracentrifugation at 48,000 x g for 3 hr. In rate zonal centrifugation of the preparation on a sucrose density gradient, the hemagglutination (HA) activity showed a sharp peak at 1.22 g/ml coinciding with the peak of infectivity. The HA activity in the peak fraction seemed to be structually associated with virus particles. After fractionation of the virus by Nonidet P-40, the HA activity was found only in the fraction of the envelope material, indicating that the hemagglutinin is situated in the viral envelop.  相似文献   

Improved hemagglutination of simbu group arboviruses with higher sodium chloride molarity diluent     

Y. Goto  Y. Inaba  H. Kurogi  E. Takahashi  K. Sato  T. Omori  T. Hanaki  H. Sazawa  M. Matumoto 《Veterinary microbiology》1976,1(4):449-458

Hemagglutination (HA) with the Simbu group arboviruses is found to be dependent on pH as well as NaCl molarity of the diluent. The current method of arbovirus HA by Clarke and Casals (1958) was modified by using a diluent with 0.4 M NaCl, 0.2 M phosphate at pH 6.0–6.2 for HA and HA inhibition with these viruses.  相似文献   

Antibodies to Akabane virus in horses,sheep and goats in Japan     

Y. Furuya  H. Shoji  Y. Inaba  M. Matumoto 《Veterinary microbiology》1980,5(3):239-242

High prevalences of neutralizing (NT) antibody to Akabane virus were obtained with horse (72%), sheep (38%) and goat (67%) serum samples collected in Chiba Prefecture, where outbreaks of abortion and congenital deformities caused by Akabane virus occurred among cattle. In these animal sera, titers of hemagglutination-inhibiting (HI) antibody to Akabane virus and of NT antibody were closely correlated.  相似文献   

IBARAKI DISEASE AND ITS RELATIONSHIP TO BLUETONGUE   总被引：3，自引：0，他引：3  

Yuji  Inaba D.V.M.  Ph.D. 《Australian veterinary journal》1975,51(4):178-185

Ibaraki disease, an epizootic disease of cattle in Japan resembling bluetongue, is characterized by fever and lesions affecting the mucous membranes, the skin, the musculature and vascular system. Degeneration of striated muscular tissue is observed in the oesophagus, larynx, pharynx, tongue and the skeletal muscles. Oedema and haemorrhage are marked in the mouth, lips, abomasum, around the coronets, etc., and are occasionally followed by degeneration of the epithelium leading to erosions or ulcerations. Severe lesions affecting the oesophageal and laryngopharyngeal musculature cause difficulty in swallowing which in turn produces dehydration and emaciation, and occasionally the aspiration pneumonia, which constitute the major causes of death of affected animals. These clinical and pathological findings indicate the similarity of the disease to bluetongue in sheep and cattle. Ibaraki disease was first recognised in Japan in 1959 and 1960. Seasonally its occurrence is limited to late summer and autumn, and geographically to the central and western parts of Japan, roughly south of 37 degrees north latitude. It is absent from the higher altitudes. The seasonal and geographical incidence suggests the possibility of an arthropod vector; but direct evidence for such a vector is still lacking. Serological data suggest the presence of Ibaraki virus on Bali Island in Indonesia and in Taiwan. The disease can be transmitted serially in calves by the intravenous inoculation of blood obtained at the height of a febrile reaction. Ibaraki virus can be isolated in bovine cell cultures from both natural and experimentally produced cases of the disease. The virus multiplies and induces cytopathic effects in primary cultures of bovine, sheep and hamster lung origin, and L cells; but it does not grow in primary cultures of horse and swine kidney nor in HeLa cell cultures. The virus is readily passaged serially in 4 to 5-day-old eggs by yolk-sac inoculation and incubation at 33.5 degrees C. It multiplies in the brains of mice of any age after incracerebral inoculation but younger mice give a better viral growth and develop encephalitis. No evidence has been obtained that rabbits and guinea pigs are susceptible to Ibaraki virus...  相似文献