Nitrogen-fixing microoganisms in paddy soils XVI     

Tadashi Katayama  Michiharu Kobayashi  Azuma Okuda 《Soil Science and Plant Nutrition》2013,59(4):31-35

1. It was proved that under aerobic shaking conditions of the system of R. capsulatus mixed with Az. agilis or R. capsulatus mixed with B. megaterium, the bacterial growth of both and the nitrogen fixation were promoted markedly, comp:ired with the non-shaking culture. However, in the system of R. capsulatus mixed with B. subtilis under aerobic shaking conditions. such remarkable stimulative effects were not recognized altough some slow effects were recognized in the nonshaking culture.

2. It was also proved that under aerobic shaking conditions of the system of R. capsulatus mixed with Az. agilis or R. capsulatus mixed with B. megaterium the assimilative activity of carbon dioxide increased over 10 times more than in the separate pure culture.

From these results, it is conceivable that a very good environment was created for the internal metabolism of R. capsulatus in such mixing systems (R-Az and R-Bm).  相似文献   

Nucleic acids in the culture fluids of Rhodopseudomonas capsulatus and other heterotrophic bacteria     

Tadashi Katayama  Michiharu Kobayashi  Azuma Okuda 《Soil Science and Plant Nutrition》2013,59(4):101-106

In previous papers (1,2), it was reported that in cultures of R. capsulatus mixed with other heterotrophic bacteria nitrogen fixation is accelerated and there is some production of slime substances (3), exchange of pyruvic acid (4–6), amino acids, carbohydrates (7) and others in these mixed systems. There is also symbiotic association between R. capsulatus and other heterotrophic bacteria (Azotobacter) in nature (8).  相似文献   

Studies on availability and fixation of phosphorus in soil (part 4)     

Tadashi Nakayama  Takashi Yamashita 《Soil Science and Plant Nutrition》2013,59(5):128-132

It Is known that inorganic phosphorus is contained in soil mainly in the form of calcium phosphates, aluminium phosphates and iron phosphates.  相似文献   

Prediction of grain protein content in winter wheat through leaf color measurements using a chlorophyll meter     

Teruo Matsunaka  Yuji Watanabe  Tadashi Miyawaki  Nobuo Ichikawa 《Soil Science and Plant Nutrition》2013,59(1):127-134

A field trial was conducted over a 3-year period at the Hokkaido Kitami Agricultural Experiment Station to examine whether the grain protein content (GPC) of a winter wheat cultivar (Triticum aestivum L. cv. Chihokukomugi) suitable for Japanese noodle-making could be predicted before harvest. The prediction of the GPC was accurate based on the color of the second leaf (just below the flag leaf) at the end of the emergence of the inflorescence, when nitrogen application was graded. In order to evaluate the reliability of this test, a survey of 95 wheat fields in the eastern part of Hokkaido was also carried out during a 3-year period. The prediction of the GPC for this cultivar based on the color of the second leaf was less accurate across many sites. The results of this survey, however, suggested that the leaf color could be used as an index for ranking the GPC as low or high in relation to processing requirements. When the leaf color value of the second leaf measured with a chlorophyll meter at the end of the emergence of the inflorescence was less than 40, it was predicted that the GPC would be lower than the processing requirement. This index could be applied to the cultivars grown in the eastern part of Hokkaido, except for those grown on peat soils.  相似文献   

Effect of presowing seed treatments on germination and early seedling growth of wheat varieties under saline conditions     

Maqsood Ahmed Kamboh  Yoko Oki  Adachi Tadashi 《Soil Science and Plant Nutrition》2013,59(1):249-255

The effect of presowing wheat seed treatments was investigated for the salt-sensitive variety Blue Silver and relatively salt-tolerant variety PARI-73 at the germination and early seedling stages. Seeds were treated with distilled water (DW) or 10/50 mm KCl, KNO₃ , CaCl₂, Ca(NO₃)₂ and then germinated in DW or 200 mm NaCl. Treatment with calcium (Ca) or potassium (K) did not lead to a significantly higher rate for final germination than the DW treatment. Ca salt treatments significantly improved shoot growth during the early seedling establishment stage in both varieties, especially in the salt-sensitive variety Blue Silver. There were significant differences in the Ca content of seeds after various presowing treatments. There were also significant differences between both varieties in the ion contents after seed treatment. However, these differences appeared to be related to the improvement of shoot growth during the early seedling establishment stage and not to the effectiveness of pres owing seed treatments in increasing germination.  相似文献   

Responses of antioxidant enzymes in the needles of hinoki cypress (Chamaecyparis obtusa) seedlings to nutrient solutions containing various calcium/aluminum ratios     

Tadashi Ogawa  Chisato Takenaka  Takafumi Tezuka 《Journal of Forest Research》2000,5(4):259-263

An attempt was made to examine the possible connection between the various ratios of calcium/aluminum (Ca/Al) in the nutrient solution of plant cultures and the active oxygen scavenging system of hinoki cypress (Chamaecyparis obtusa) seedlings. The hinoki cypress seedlings were transferred to nutrient solutions containing 5 mM AlCl₃ together with various concentrations of Ca(NO₃)₂ in pots containing glass beads and Teflon tips. The activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) in the needles were estimated at several stages during the 7-day treatment. The samples treated with the lower Ca/Al solutions show the highest SOD activities. The activities of APX and GR, both of them involved in the ascorbate-glutathione cycle, showed the same tendency with decrease to higher Ca/Al ratio. These results indicate that rhizospheric Ca might compete with Al and ameliorate Al toxicity on and in the roots, the Al stress is not transformed to the needles after a few days, and the ascorbate-glutathione cycle in the hinoki cypress needles might fluctuate and be suppressed by the rhizospheric Al stress during the 7 days. This work was supported in part by funding from the Japan Science & Technology Corporation, the CREST program 1996–2001, and the Center for Forest Decline Studies.  相似文献   

Automatic detection of a damaged cutting tool during machining I: method to detect damaged bandsaw teeth during sawing     

Nanfeng Zhu  Chiaki Tanaka  Tadashi Ohtani  Hiroshi Usuki 《Journal of Wood Science》2000,46(6):437-443

This paper presents an on-line method for detecting damaged teeth in the bandsaw using acoustic emission (AE) signal energy. The method is based on an analysis of differences in AE energies generated by normal and damaged teeth during sawing. Because of the difference in the amount of sawing, the AE energy was low for sawing by the damaged tooth and high for sawing by the normal tooth immediately after the damaged tooth. The ratio of AE energy for two successive teeth — a normal tooth immediately following a damaged tooth — was much greater than 1, whereas the ratio of AE energy for two successive normal teeth was close to 1. The results demonstrate that the technique using the AE energy ratio for two successive teeth is effective for on-line detection of damaged bandsaw teeth.Part of this paper was presented at the 9th Annual Meeting of the Chugoku Skikoku Branch of the Japan Wood Research Society, Tottori, October 1997  相似文献   

Purification and characterization of two anionic trypsins from the hepatopancreas of carp   总被引：8，自引：0，他引：8  

Min-Jie Cao  Kiyoshi Osatomi  Miho Suzuki  Kenji Hara  Katsuyasu Tachibana and  Tadashi Ishihara 《Fisheries Science》2000,66(6):1172-1179

SUMMARY: Two trypsins, designated as trypsin A and trypsin B, have been purified from the hepatopancreas of carp. The purification procedures consisted of ammonium sulfate fractionation, and chromatographies on DEAE-Sephacel, Ultrogel AcA54 and Q-Sepharose. Trypsin A was purified to homogeneity with the molecular mass of approximately 28 kDa, while trypsin B gave two close bands of 28.5 kDa and 28 kDa on sodium dodecylsulfate polyacrylamide gel electrophoresis both under reducing and non-reducing conditions. On native-PAGE, both trypsin A and trypsin B showed a single band. Trypsin A and trypsin B revealed optimum temperature of 40°C and 45°C, respectively, and shared the same optimum pH 9.0 using Boc-Phe-Ser-Arg-MCA as substrate. Both enzymes were effectively inhibited by trypsin inhibitors and their susceptibilities were similar. The NH₂-terminal amino acid sequences of trypsin A and trypsin B were determined to 37th and 40th amino acid residue, respectively. Their sequences were very homologous, but not identical to that of a trypsin-type serine proteinase from carp muscle and these of other trypsins. Immunoblotting test using the antibody raised against trypsin A cross-reacted with trypsin B positively.  相似文献   

Mesh selectivity of a sweeping trammel net for Japanese whiting Sillago japonica   总被引：2，自引：0，他引：2  

Ari Purbayanto  Seiji Akiyama  Tadashi Tokai and  Takafumi Arimoto 《Fisheries Science》2000,66(1):97-103

  相似文献   

Synthesis and Structure–Activity Relationships of Miticidal 4,5-Dihydropyrazole-5-thiones     

Isao Tada  Minoru Motoki  Nobuyoshi Takahashi  Tetsuji Miyata  Tomoko Takechi  Toshiro Uchida  Yasushi Takagi 《Pest management science》1996,48(2):165-173

A series of novel 4,5-dihydropyrazole-5-thiones (DHPs) was synthesised by treating the corresponding dihydropyrazolones with ‘Lawesson’s reagent and evaluated for miticidal activity against two-spotted spider mites (Tetranychus urticae Koch). Of these, 3-(4-chlorophenyl)-4,4-dimethyl-1-phenyl-4,5-dihydropyrazole-5-thione, 3-(4-chlorophenyl)-4-ethyl-4-methyl-1-phenyl-4,5-dihydropyrazole-5-thione, 3-(4-chlorophenyl)-1-phenyl-4,5-dihydropyrazole-5-thione-4-spirocyclopentane and 4,4-dimethyl-1-phenyl-3-(4-trifluoromethyl-phenyl)-4,5-dihydropyrazole-5-thione were highly active (pEC₅₀>4·0) and were more effective than the miticide dicofol (pEC₅₀=3·879), which has traditionally been used for the control of phytophagous mites. Structure–activity relationship (SAR) studies were performed on each position of the pyrazole ring of DHPs. The results indicated that the unsubstituted phenyl, 4-substituted phenyl and thioxo groups on the 1-, 3- and 5-positions of DHPs respectively were required for activity. Quantitative SAR studies using physicochemical parameters of substituents and the capacity factor k′ as a hydrophobicity index suggested that: (a) the activities of all types of DHPs examined were mainly dominated by hydrophobicity, (b) the bulkiness of 4-substituents of the 3-phenyl ring favoured the activity and (c) the log k′ optimum for all DHPs was 1·675, equivalent to a log P_ow value of c. 5·0.  相似文献