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31.
Chhourn Orn Rieko Shishido Masahiro Akimoto Ryo Ishikawa Than Myint Htun Ken-Ichi Nonomura Yohei Koide Men Sarom Seng Vang Sakhan Sophany Ouk Makara Takashige Ishii 《Breeding Science》2015,65(5):430-437
A total of 448 samples in five natural populations of wild rice (Oryza rufipogon) were collected in Cambodia. They were examined using 12 SSR and two chloroplast markers to evaluate the degree of variation among populations and the genetic structure within populations. In the two annual populations, the number of plants with homozygous alleles at all 12 SSR loci were high (66.3% and 79.5%), suggesting that these plants propagate mainly through self-pollination. In the three perennial populations, no individuals had all homozygous genotypes, but redundant genotypes resulted from clonal propagation were observed. Percentages of the redundant genotypes were highly varied (3.6%, 29.2% and 86.0%). This may be due to the different stable levels of environmental conditions. As for chloroplast genome, most of the wild plants showed the same chloroplast types as most Indica-type cultivars have. However, plants with different chloroplast types were maintained, even in the same population. In tropical Asian countries, many wild rice populations were observed under similar ecological conditions examined in this study. Therefore, the present results concerning population structure will be important to further elucidate genetic features of wild rice, and will also give strong clues to utilize and conserve wild natural genetic resources. 相似文献
32.
Synthesis of haptens for development of antibodies to alkylphenols and evaluation and optimization of a selected antibody for ELISA development 总被引:1,自引:0,他引:1
Watanabe E Eun H Baba K Arao T Endo S Ueji M Ishii Y 《Journal of agricultural and food chemistry》2005,53(19):7395-7403
The development of an enzyme-linked immunosorbent assay (ELISA) based on polyclonal antibodies for a class of endocrine disrupting compounds, 4-nonylphenol, is described. The parent molecule was derivatized at the ortho position of the free phenolic hydroxyl group to obtain the hapten, NP1, and it was conjugated with keyhole limpet hemocyanin, which was used as an immunogen. Four antisera were generated and screened against three coating antigens. The most sensitive ELISA from the screening tests (antiserum NP03As, 1/1000, and coating antigen NP1-BSA, 1 microg/mL) was further optimized and characterized. The influence of various physicochemical factors (organic solvent, pH, ion strength) was investigated. Methanol as the additive organic solvent was found to be the best organic solvent for the ELISA, with optimal sensitivity observed at a concentration of 5%. The ELISA parameters were changed at more acidic or basic pH values, whereas higher ionic strengths strongly suppressed the I(50) value and the maximum absorbance. The most sensitive ELISA for 4-nonylphenol exhibited an I(50) value of 38.6 +/- 5.5 microg/L, with a dynamic range from 12 to 350 microg/L, and the lower limit of detection was 7.7 +/- 1.3 microg/L. The optimized ELISA displayed no significant cross-reaction against the parent compounds, nonylphenol ethoxylates, degradation products, carboxylates, and bisphenol A, except in 4-octylphenol. 相似文献
33.
34.
Takashi Yamasaki Ayumi Enomoto Atsushi Kato Tadashi Ishii Kazumasa Shimizu 《Journal of Wood Science》2011,57(1):76-84
Arabinoglucuronoxylans (AGXs) isolated from the holocellulose of sugi (Cryptomeria japonica) and hinoki (Chamaecyparis obtusa) contained one 4-O-methyl-d-glucopyranosyluronic acid (4-O-Me-d-GlcAp) residue per 6.2 d-xylopyranose (d-Xylp) residues and one 4-O-Me-d-GlcAp residue per 3.8 d-Xylp residues. These AGXs were subjected to partial acid hydrolysis. Analyses by size exclusion chromatography and electrospray-ionization
mass spectroscopy of the neutral sugar fractions in the hydrolysates showed the presence of xylooligosaccharides having a
degree of polymerization of 2-8 in addition to d-Xyl, suggesting that the AGXs from sugi and hinoki contained unsubstituted chains consisting of at least eight d-Xyl residues. The acidic sugars in the hydrolysates were separated into two series of aldouronic acids composed of 4-O-Me-d-GlcAp and d-Xylp by ion-exchange chromatography. The first series included aldouronic acids from aldobiouronic acid (4-O-Me-d-GlcAp-Xyl) to aldopentaouronic acids (4-O-Me-d-GlcAp-Xyl4). The second series were aldouronic acids composed of two 4-O-Me-d-GlcAp residues and 2-4 d-Xyl residues. In these acidic sugars, the uronic acid side chains were located on two contiguous d-Xyl residues. These facts indicated that AGXs from sugi and hinoki had a structural unit containing two 4-O-Me-d-GlcAp residues on two contiguous d-Xyl residues as well as AGXs from spruce and larch. 相似文献
35.
Watanabe E Eun H Baba K Arao T Ishii Y Endo S Ueji M 《Journal of agricultural and food chemistry》2004,52(10):2756-2762
The performance of a commercially available microtiter plate ELISA kit for the determination of the neonicotinoid insecticide imidacloprid was evaluated for sensitivity, selectivity, influence of organic solvent used for extraction procedure, matrix interference originated from agricultural sample, accuracy, and method comparison with conventional HPLC analysis. The limit of detection for the kit (0.1 or 0.5 ng/mL) was determined. The working range (1-39 ng/mL) experimentally calculated on the basis of a criterion, which is determined as the range from I(20) to I(80), was comparable to that established by the manufacturer (1-50 ng/mL). The linearity of the standard curve based on the kit-assembled standard solutions agreed with the one based on the self-made standard solutions. Specificity studies indicate that the imidacloprid monoclonal antibody can readily distinguish the target compound from other structurally related neonicotinoid analogues and some metabolites, with the exception of clothianidin, the cross-reactivity of which was approximately 12%. To extract imidacloprid from an agricultural sample (apple) as simply and rapidly as possible, some extraction methods were examined. Consequently, the extraction method with hand-shaking for 5 min was the best among the examined methods. For the analysis of imidacloprid in apple samples, it was extracted directly with methanol and the extracts were diluted 10-fold (100-fold in the well) with water prior to ELISA analysis. No significant matrix interference was observed with the dilution factor. Recoveries of imidacloprid from fortified apple samples ranged from 87.7 to 112.0%. The results obtained with the ELISA kit correlated well with those by the reference method (conventional HPLC analysis) for apple samples (r > 0.998). These findings strongly indicate that the ELISA kit may be employed routinely for an on-site imidacloprid residue analysis of apple samples. 相似文献
36.
Kazuyuki?Mori Yu?Sakamoto Nobuhiro?Mukojima Seiji?Tamiya Takashi?Nakao Takashige?Ishii Kazuyoshi?HosakaEmail author 《Euphytica》2011,180(3):347-355
Multiplex PCR is practically a reasonable choice for molecular marker-assisted selection in potato breeding. We had developed
and were using a multiplex PCR method for selection of resistance genes to cyst nematode (H1), Potato virus X (Rx1) and late blight (R1 and R2). Since then, more reliable and tightly linked markers for H1 and R2, and a new marker for resistance to Potato virus Y (Ry
chc
) were developed. In this article, all these superior markers, including a positive marker to eliminate PCR-failed samples,
were incorporated into one multiplex PCR assay. Using the newly developed multiplex PCR technique, five plants potentially
harboring all five resistance genes were selected from 96 hybrid plants approximately 5 h after DNA extraction, which is a
third of the operation time compared with separate PCR reactions for each marker. 相似文献
37.
Binding of the Sp1 transcription factor by the human Harvey ras1 proto-oncogene promoter 总被引:34,自引:0,他引:34
S Ishii J T Kadonaga R Tjian J N Brady G T Merlino I Pastan 《Science (New York, N.Y.)》1986,232(4756):1410-1413
38.
The metabolism of phosphoinositide-derived messenger molecules 总被引:64,自引:0,他引:64
P W Majerus T M Connolly H Deckmyn T S Ross T E Bross H Ishii V S Bansal D B Wilson 《Science (New York, N.Y.)》1986,234(4783):1519-1526
The phosphoinositides are minor phospholipids present in all eukaryotic cells. They are storage forms for messenger molecules that transmit signals across the cell membrane and evoke responses to extracellular agonists. The phosphoinositides break down to liberate messenger molecules or precursors of messenger molecules. Many different compounds are formed, although the functions of only a few are understood. Recent studies elaborating the pathways for formation of products from phosphoinositides and the factors controlling their metabolism are summarized here. 相似文献
39.
A MicroRNA feedback circuit in midbrain dopamine neurons 总被引:4,自引:0,他引:4
Kim J Inoue K Ishii J Vanti WB Voronov SV Murchison E Hannon G Abeliovich A 《Science (New York, N.Y.)》2007,317(5842):1220-1224
40.
Rice Full-Length cDNA Consortium;National Institute of Agrobiological Sciences Rice Full-Length cDNA Project Team Kikuchi S Satoh K Nagata T Kawagashira N Doi K Kishimoto N Yazaki J Ishikawa M Yamada H Ooka H Hotta I Kojima K Namiki T Ohneda E Yahagi W Suzuki K Li CJ Ohtsuki K Shishiki T;Foundation of Advancement of International Science Genome Sequencing & Analysis Group Otomo Y Murakami K Iida Y Sugano S Fujimura T Suzuki Y Tsunoda Y Kurosaki T Kodama T Masuda H Kobayashi M Xie Q Lu M 《Science (New York, N.Y.)》2003,301(5631):376-379