Effect of calcium ion on the thermal denaturation of subfragment-1 and rod regions of squid myosin upon the heating of myofibrils     

Kazuyo Abe  Yasunori Kinoshita  Kunihiko Konno 《Fisheries Science》2012,78(1):163-168

Thermal inactivation of Ca²⁺ ATPase of squid myofibrils was significantly suppressed in the presence of Ca²⁺. Monomeric myosin content decreased much faster than Ca²⁺ ATPase inactivation in Ca medium, which was well explained by fast rod denaturation. In contrast, rod denaturation was slower than S-1 in EDTA medium. The decrease in monomeric myosin content was explained by faster S-1 denaturation. Comparing the S-1 and rod denaturation rates at a fixed temperature, it was concluded that S-1 denaturation was suppressed by Ca²⁺ whereas the rod denaturation was not. An unfolding experiment with isolated myosin rod confirmed that there was no stabilizing effect of Ca²⁺ on myosin rod. It was concluded that significant stabilization of the S-1 portion by Ca²⁺ generated the apparently different myosin denaturation patterns in the two media.  相似文献   

Nitrate removal efficiency and bacterial community of polycaprolactone-packed bioreactors treating water from a recirculating aquaculture system     

Guozhi Luo  Zefeng Liu  Jinfang Gao  Zhiwei Hou  Hongxin Tan 《Aquaculture International》2018,26(3):773-784

A denitrification reactor packed with polycaprolactone (PCL) as a carbon source and biofilm carrier was developed to remove nitrate nitrogen (\( {\mathrm{NO}}_3^{-}-\mathrm{N} \)) from the water of a recirculating aquaculture system for 115 days. The hydraulic retention time was set to 6 h, and the water flow rate was 0.95 L h^?1. The removal rates of \( {\mathrm{NO}}_3^{-}-\mathrm{N} \) ranged from 34.67 to 155.7 g \( {\mathrm{NO}}_3^{-}-\mathrm{N} \) m^?3 day^?1 when the effluent \( {\mathrm{NO}}_3^{-}-\mathrm{N} \) concentrations ranged between 1.62 and 72.25 mg L^?1. No obvious changes in the Fourier transform infrared spectra of the PCL before and after use were observed. Bacterial community structure in the biofilm of the PCL granules was analyzed using Illumina MiSeq sequencing technology. Proteobacteria and Bacteroidetes were predominant in the biofilm, with relative abundances of 63.9 and 27.4%, respectively. Three genera, Acidovorax, Azospira, and Diaphorobacter, were capable of both denitrification and PCL degradation. This study indicates that PCL-packed reactors may be used and optimized for removing nitrate from aquaculture effluents.  相似文献   

Plasma levels of immune factors and sex steroids in the male seahorse <Emphasis Type="Italic">Hippocampus erectus</Emphasis> during a breeding cycle     

Tingting Lin  Xin Liu  Dongxue Xiao  Dong Zhang 《Fish physiology and biochemistry》2017,43(3):889-899

To better understand the endocrine- and immune-response pattern during reproduction in a fish species having parental care behaviors and also to accumulate the endocrine- and immune-related data for future explanations of the low reproductive efficiency in seahorse species, the variations of immune factors and sex steroids in the plasma of the male lined seahorse Hippocampus erectus at different breeding stages, i.e., pre-pregnancy, pregnancy (early, middle, and late periods), and post-pregnancy, were investigated in the present study. The immune factors included monocytes/leucocytes (M/L), leucocyte phagocytic rate (LPR), immunoglobulin M (Ig M), interleukin-2 (IL-2), interferon-α (IFN-α), and lysozyme (LZM). The sex steroids included testosterone (T), 11-ketotestosterone (11-KT), 11β-hydroxytestosterone (11β-OHT), 17α-methyltestosterone (17α-MT), 17β-estradiol (E2), and 17α-hydroxy-20β-dihydroprogesterone (17α-20β-P). Moreover, the immune metabolic activity of epithelium cells in the brood pouch at different breeding stages was also analyzed through ultrastructural observations of the abundance of cytoplasmic granules, mitochondria, endoplasmic reticulum, lysosomes, and exocytosis. The results show that a higher immune level was observed during pregnancy, particularly in the early and middle periods, and a lower immune level was noted during pre-pregnancy. Correspondingly, the epithelium cells in the brood pouch also showed a stronger immune metabolic activity during pregnancy and weaker activity during pre-pregnancy. Four sex steroids of T, 11β-OHT, 17α-MT, and E2 were higher during pre-pregnancy and lower during post-pregnancy, whereas 11-KT and 17α-20β-P, which were positively correlated with part immune factors, were higher during pregnancy. No negative correlations between sex steroids and immune factors were observed. In conclusion, the higher immune competence during pregnancy may indicate that parental care could improve immunity, which may be the major factor for no immunosuppressive effect of sex steroids during reproduction in the seahorse H. erectus, unlike noncaregiving fishes in which inhibitions of sex steroids on immunity are frequently observed. Moreover, higher 11-KT and 17α-20β-P during pregnancy than during pre-pregnancy and post-pregnancy may suggest that these two steroids are also involved in parental care regulation.  相似文献   

Comparative analysis of using cereal grains and compound feed in semi-intensive common carp pond production     

Zoran Marković  Marko Stanković  Božidar Rašković  Zorka Dulić  Ivana Živić  Vesna Poleksić 《Aquaculture International》2016,24(6):1699-1723

The semi-intensive system of carp farming is dominant in inland aquaculture. Although it appears to be simple, this system has a whole range of options, from those based on traditional rearing methods with use of plants from the region where the fish are reared to production based on the use of high-quality concentrated feeds. In this paper, we conduct a comparative analysis of the effect of using cereal grains as opposed to compound feed (pelleted and extruded) on the quantity of fish produced, flesh quality, histological changes in the digestive tract, water quality, and structure of the biocoenosis in the pond ecosystem. When the fish are fed cereal grains, production ranges from 500 to 1500 kg/ha, while using compound feed gives production that is twice as great. The proportions of protein and water are higher and that of lipids lower in flesh of common carp reared on compound feed in relation to fish fed cereal grains. Use of extruded feed gives better content of n-3 polyunsaturated fatty acids and a better n-3/n-6 ratio than when pelleted feed and cereal grains are used. Comparison of water quality in carp ponds under conditions of using various types of feed indicates that there are no significant differences. Although it was expected that feed with a higher percentage of proteins would cause disturbances in the aquatic ecosystem, their continual incorporation into the cycling of matter in the water contributes to maintenance of ecological equilibrium and even indirectly promotes growth of the common carp.  相似文献   

Post-molt processes of cuticle formation and calcification in the Japanese mitten crab <Emphasis Type="Italic">Eriocheir japonicus</Emphasis>     

Konomi Sakamoto  Wataru Honto  Masaharu Iguchi  Nobuhiro Ogawa  Kazuhiro Ura  Yasuaki Takagi 《Fisheries Science》2009,75(1):91-98

This study examines the following in the Japanese mitten crab: (1) the structure of the exoskeleton with special reference to its calcification; (2) the progression of post-molt cuticle formation and calcification. In the crab, the structure and calcification state of the exoskeleton at the molt and during the inter-molt stage were similar to those of other crustaceans. During the inter-molt, the exoskeleton consisted of four cuticle layers; the outermost epicuticle, the exocuticle, the endocuticle and the innermost membrane layer. Intense calcification was observed in the exo- and endocuticle. At the molt, the synthesis of the epi- and exocuticle was already complete, and the addition of the endocuticle began after the molt. Calcification of the exocuticle initiated soon after the molt, but there was a delay between endocuticle matrix synthesis and calcification. Histology showed that the process of calcification was similar to that in other crustaceans. However, calcium concentrations within the exoskeleton continued to increase and never reached the levels of the inter-molt stage at the end of the experiment. This suggests that the Japanese mitten crab is relatively slow to calcify compared to other crustaceans.  相似文献   

Polymorphisms in the 5′ flanking region of the insulin-like growth factor I gene are associated with growth traits in largemouth bass <Emphasis Type="Italic">Micropterus salmoides</Emphasis>     

Xiao Hui Li  Jun Jie Bai  Xing Ye  Yin Chang Hu  Sheng Jie Li  Ling Yun Yu 《Fisheries Science》2009,75(2):351-358

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Relative potencies of natural estrogens on vitellogenin and choriogenin levels in the Indian freshwater spotted snakehead, <Emphasis Type="Italic">Channa punctata</Emphasis>: in vivo and in vitro studies     

K. V. Rani  N. Sehgal  S. V. Goswami  Om Prakash 《Fish physiology and biochemistry》2010,36(3):587-595

The relative efficacies of three natural estrogens viz., estrone (E₁), estradiol-17β (E₂) and estriol (E₃) to induce synthesis of vitellogenin (Vg) and choriogenin (Chg) were assessed in primary hepatocyte cultures of the Indian freshwater spotted snakehead, Channa punctata. Hepatocytes were isolated from the spotted snakehead liver by a non-enzymatic protocol. Optimum culture conditions were standardized for ensuring their viability and functioning. Isolated hepatocytes were cultured for 48 h for monolayer formation and then exposed to various concentrations (0.001–10 μM) of the three estrogens. Competitive homologous ELISAs, developed and validated for spotted snakehead Vg and Chg were employed to determine the amounts of these two proteins secreted into the culture medium after 48 h of incubation. The results reveal that although all the three estrogens were effective in inducing the production of Vg and Chg in a dose-dependent manner, there were differences in their relative potencies. Of three estrogens, E₁ was the least potent and could induce synthesis of Vg and Chg only at a minimum concentration of 0.5 μM; whereas significant levels of both the proteins were quantified in culture medium by exposing the hepatocytes to E₂ or E₃ even at a concentration of 0.001 μM. All three estrogens were effective in inducing synthesis of Vg and Chg in vivo also. These results suggest the possibility of employing the above in vitro experimental design to monitor the presence of estrogens/estrogen-like chemicals in natural waters, which could interfere with the estrogen receptor system of fish. This study further points to the possibility of using Chg, in addition to Vg, as a parameter for screening various chemicals for their estrogenic activity.  相似文献   

Guanine-cytosine contents of the host and symbiont cDNA in a symbiotic coral     

Shin -Ichi Kii  Jiro Tanaka  Toshiki Watanabe 《Fisheries Science》2007,73(6):1362-1372

ABSTRACT:   Hermatypic (reef-building) corals harbor dinoflagellate endo-symbionts Symbiodinium spp. In studying gene expression in such symbiotic corals, problems arise regarding how to distinguish the coral and symbiont mRNA, and how to estimate their fractions in the mRNA population of the holobiont (symbiotic complex of the coral and Symbiodinium cells). In this study, these issues were addressed using juveniles of hermatypic coral Acropora tenuis in symbiosis with Symbiodinium cells of strain PL-TS-1. First, the guanine-cytosine (GC) contents were determined in expressed sequence tags (EST) from PL-TS-1 cells cultured in vitro and symbiont-free larvae of A. tenuis , and their average GC contents were found to be significantly different. The average GC content of the EST from the holobiont was much closer to that of A. tenuis larvae, suggesting that the majority (>90%) of mRNA isolated from the holobiont originated in the host. In protein-coding sequences, little overlap was observed between the GC-content distributions of PL-TS-1 cells and A. tenuis larvae. All of the coding sequences ( n  = 59) found in the A. tenuis EST had GC contents below 0.5, whereas the GC content exceeded 0.5 in the majority (43/44) of coding sequences from the nuclear genome of PL-TS-1 cells.  相似文献   

Microsatellite marker isolation and cultured strain identification in <Emphasis Type="Italic">Carassius auratus gibelio</Emphasis>     

Wei Guo  Jian-Fang Gui 《Aquaculture International》2008,16(6):497-510

Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp.  相似文献   

Relationship between zooplankton abundance and the early marine life history of juvenile chum salmon <Emphasis Type="Italic">Oncorhynchus keta</Emphasis> in eastern Hokkaido,Japan     

Toshihiko Saito  Ikutaro Shimizu  Jiro Seki  Kazuya Nagasawa 《Fisheries Science》2009,75(2):303-316

Interannual variations in abundance, timing of outmigration from rivers, growth rate and condition of juvenile chum salmon (Oncorhynchus keta) were studied in the Nemuro Strait (eastern Hokkaido, Japan) during 1999–2002 to establish a possible relationship to zooplankton abundance. The otolith microstructure of juveniles was examined each year in late June to determine their time and size at sea entry (i.e., outmigration), and to estimate the early marine growth rates. Salmon outmigration peaked in mid- or late May, which coincided, in three of the four study years, with the peak release of juveniles into rivers within the study area. Abundance, growth rate and condition of fish were higher in 2001, when—compared to other years—smaller fish experienced higher growth rates, coinciding with greater zooplankton abundance for that year. Our results suggest that high zooplankton abundance positively influenced juvenile chum salmon growth and the condition of the fish during their early marine life despite their small size at sea entry.  相似文献