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51.
52.
Establishment of a Model of Spontaneously-Running-Tokushima-Shikoku Rats with Left Atrial Thrombosis
Takamasa Ohnishi Fumiko Hisaoka Masaki Morishima Akira Takahashi Nagakatsu Harada Kazuaki Mawatari Hidekazu Arai Emiko Yoshioka Satomi Toda Izumi Keisuke Yutaka Nakaya 《Journal of toxicologic pathology》2014,27(1):51-56
Studies that investigate the underlying mechanisms of disease and treatment options typically require the use of a suitable animal model. Few suitable animal models exist for left atrial thrombosis. Here, we demonstrated that the Spontaneously-Running-Tokushima-Shikoku (SPORTS) rat — a Wistar strain known for its running ability—is predisposed to the development of thrombi in the left atrium. We investigated the incidence of left atrial thrombosis in male (n = 16) and female (n = 17) SPORTS rats and observed organized atrial thrombosis in 57% and 38% of males and female rats, respectively. In the male rats, systolic blood pressures and heart rates were significantly higher in SPORTS rats than in control Wistar rats. We could not find any evidence of arrhythmias, such as atrial fibrillation, during electrocardiographic examination of SPORTS rats. We believe that the SPORTS rat could serve as a new research model for left atrial thrombosis; further, it may be suitable for research investigating the development of new antithrombotic approaches for the control of atrial thrombosis or familial thrombophilia in humans. 相似文献
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Toshio Akiyama Takeshi Murai Yasuhiro Hirasawa Takeshi Nose 《Aquaculture (Amsterdam, Netherlands)》1984,37(3):217-222
In a 6-week feeding experiment, chum salmon, Oncorhynchus keta, swim-up fry, were fed fish meal diets supplemented with silkworm pupae powder (5%), dried beef liver (5%), krill meal (5%) or earthworm powder (5%) at the expense of fish meal, or substituting glucose (13%) for dextrin. Fish fed the diet with earthworm powder showed the best growth performance (675% weight gain in 6 weeks) and feed efficiency (117%). The growth rate and feed efficiency of the krill meal group were also significantly better than those of the control group (without supplement or substitution). Substitution of glucose and supplementation of silkworm pupae or beef liver failed to improve the growth rate, but significantly improved feed efficiency. Food consumption of fish fed the diet supplemented with earthworms was lowest, and none of the dietary treatments appreciably increased food intake of the fish over that of the control group. The dietary group receiving earthworm powder also showed significantly higher body fat content than the remaining dietary groups. No significant differences were noted in body protein and ash contents among all dietary treatments. 相似文献
54.
Effects of high dose of vitamin A on reproduction and egg quality of Japanese flounder Paralichthys olivaceus 总被引:2,自引:0,他引:2
Hirofumi Furuita Hideki Tanaka Takeshi Yamamoto Manabu Shiraishi Toshio Takeuchi 《Fisheries Science》2001,67(4):606-613
ABSTRACT: The present study was conducted to investigate the effects of dietary vitamin A on reproduction and egg quality in Japanese flounder Paralichthys olivaceus . Broodstock were fed experimental pellets containing two levels of vitamin A [11 × 103 IU/100 g (control diet; CD), 337 × 103 IU/100 g (experimental diet; ED)] for approximately 2 months before spawning and during the spawning period. Two groups of five females (average weight 1.4 kg) and 10 males (average weight 0.7 kg) were randomly allocated to two 30 m3 indoor tanks. Total egg production of the CD group was slightly higher than the ED group. Percentage of buoyant eggs and hatching rate of the ED group were significantly higher than the CD. In other egg quality parameters, such as percentage abnormal larvae and starvation tolerance of larvae, no notable difference was found between these two groups. At the end of the experiment, the skin color of broodstock in the ED group was darker than that of the CD group. Vitamin A content in eggs of the ED group was significantly higher than that of the CD group. However, the difference in vitamin A content in eggs between the ED and CD groups was much smaller than that in the liver of the females between the two groups. These results indicate that feeding broodstock a higher level of vitamin A increases the vitamin A content in eggs but does not affect egg quality in Japanese flounder because excess dietary vitamin A was stored mainly in the broodstocks' liver. 相似文献
55.
ABSTRACT: Sporophyll formation in two alariaceous plants, Undaria pinnatifida and Alaria crassifolia , was studied in relation to the nutrient requirements. The sporophylls of U. pinnatifida formed zoosporangia when they had N and P contents greater than 1.4 kgN/m3 and 0.74 kgP/m3 . This indicates that these values are the critical nutrient levels for zoospore formation in U. pinnatifida . In the sporophytes of A. crassifolia , many sporophylls with zoosporangia showed nutrient contents higher than 6.25 kgN/m3 and 1.70 kgP/m3 . These results suggest that the U. pinnatifida can form zoospores at lower levels of N and P contents than A. crassifolia . Both species often formed zoosporangial sori on the blades in the late period of each reproductive season. The fertile parts of the blade showed N and P contents higher than the critical levels. This phenomenon indicates that the blades have the capability to form zoosporangial sori if there is a sufficient accumulation of nutrients for zoospore formation. The zoospore formation on the blade seems to be accomplished by an overflow of excess nutrients from sporophylls into the blade, or by accumulating sufficient nutrients to form sori even if the sporophylls are not formed. 相似文献
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Spermatogenesis and its endocrine regulation 总被引:2,自引:2,他引:2
Three major phases compose spermatogenesis: mitotic proliferation of spermatogonia, meiosis of spermatocytes, and spermiogenesis,
the restructuring of spermatids into flagellated spermatozoa. The process is fuelled by stem cells that, when dividing, either
self-renew or produce spermatogonia that are committed to proliferation, meiosis, and spermiogenesis. During all phases, germ
cells are in close contact with and require the structural and functional support of Sertoli cells. In contrast to germ cells,
these somatic cells express receptors for sex steroids and follicle-stimulating hormone (FSH), the most important hormones
that regulate spermatogenesis. A typical Sertoli cell response to an endocrine stimulus would be to change the release of
a growth factor that would then mediate the hormone's effect to the germ cells. Recent studies in the Japanese eel have shown,
for example, that in the absence of gonadotropin Sertoli cells produce a growth factor (an orthologue of anti-Müllerian hormone)
that restricts stem cell divisions to the self-renewal pathway; also estrogens stimulate stem cell renewal divisions but not
spermatogonial proliferation. Gonadotropin or 11-ketotestosterone (11-KT) stimulation, however, induces spermatogonial proliferation,
which is in part mimicked by another Sertoli cell-derived growth factor (activin B). Since FSH (besides luteinizing hormone,
LH) stimulates steroidogenesis in fish, and since FSH is the only gonadotropin detected in the plasma of sexually immature
salmonids, increased FSH signalling may be sufficient to initiate spermatogenesis by activating both Sertoli cell functions
and 11-KT production. Another important androgen is testosterone (T), which seems to act via feedback mechanisms that can
compromise FSH-dependent signalling or steroidogenesis. The testicular production of T and 11-KT therefore needs to be balanced
adequately. Further research is required to elucidate in what way(s) 11-KT stimulates later stages of development, such as
entry into meiosis and spermiogenesis. At this period, LH becomes increasingly important for the regulation of androgen production.
Results from mammalian models suggest that during the later phases, the control of germ cell apoptosis via Sertoli cell factors
is an important regulatory mechanism. In many species, sperm cells cannot fertilize eggs until having passed a maturation
process known as capacitation, which includes the acquisition of motility. Progestins that are produced under the influence
of LH appear to play an important role in this context, which involves the control of the composition of the seminal plasma
(e.g., pH values).
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献