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81.
异噻唑类杂环化合物具有广泛的生物活性。为了扩大先导结构的发现范围,以3,4-二氯异噻唑-5-甲酸为原料合成了10个未见文献报道的3,4-二氯异噻唑-5-甲酰胺衍生物;其结构经核磁共振氢谱、红外光谱和元素分析确证。初步生物测试结果表明,化合物Ⅲe对9种病原真菌表现出了广谱的抑菌活性,在50 μg/mL下其抑制率均超过50%;在100 μg/mL下,大部分化合物展现了较好的抗烟草花叶病毒(tobacco mosaic virus,TMV)或诱导烟草抗TMV活性,其中化合物Ⅲa和Ⅲi的诱导活性分别为41.88%和42.92%。  相似文献   
82.
In the present study, the temporal and spatial variation of the abundance of the alkane monooxygenase gene alkB and 16S rRNA genes in different soil compartments was analysed in the presence or absence of 2-methyl-4-chlorophenoxyacetic acid (MCPA) after the addition of pea litter to soil in a microcosm study. Samples were analysed shortly after litter addition (T0) and 1?week (T1), 3?weeks (T3) and 6?weeks (T6) after the addition of litter. In addition also, the quantity and quality of litter-derived alkanes was analysed and measured. The results revealed a fast and complete degradation of MCPA in all compartments throughout the experiment. Nevertheless, significant changes in the distribution patterns of short- and middle-chained alkanes suggest an interaction of MCPA and alkane degradation. alkB gene copy numbers were highly influenced by the time point of analysis and by the investigated soil compartment. Overall, an increase in alkB gene copy numbers from T0 to T3 was visible in the upper soil compartments whereas a decrease compared to T0 was measured in the deeper soil compartments. MCPA addition resulted in an increase of alkB abundance at T6. Gene copy numbers of 16S rRNA were not influenced by sampling time and soil compartment. In contrast to the control treatments, a slight increase in 16S rRNA gene copy numbers was visible at T1 and T3 compared to T0 in all soil compartments.  相似文献   
83.
Tree canopies are believed to act as a sink of atmospheric ammonia (NH3). However, few studies have compared the uptake efficiency of different tree species. This study assessed the uptake of 15N-labelled NH3 at 5, 20, 50 and 100?ppbv by leaves and twigs of potted silver birch, European beech, pedunculate oak and Scots pine saplings in June, August and September 2008. Additionally, foliar uptake of 13C-labelled carbon dioxide (13CO2) and leaf stomatal characteristics were determined per species and treatment date and the relation with 15NH3 uptake and estimated stomatal 15NH3 uptake were assessed. Both 15NH3 and 13CO2 uptake were affected by tree species and treatment date, but only 15NH3 uptake was influenced by the applied NH3 concentration. Depending on the treatment date, 15NH3 uptake by leaves and twigs was highest at 5 (September), 20 (June) or 50 (August) ppbv. Birch, beech and oak leaves showed the highest uptake in August, while for pine needles this was in June and, except at 5?ppbv in June, the 15NH3 uptake was always higher for the deciduous species than for pine. For all species except beech 13CO2 uptake was highest in August and on every treatment date the 13CO2 uptake by leaves of deciduous species was significantly higher than by pine needles. Leaf characteristics and 13CO2 uptake did not provide a strong explanation for the observed differences in 15NH3 uptake. This study shows that on the short-term a high interspecific variability exists in NH3 uptake, which depends on the time in the growing season.  相似文献   
84.
The susceptibility of larvae ofThaumetopoea solitaria Freyer (Lepidoptera; Notodontidae, Thaumetopoeinae), fed on treated pistachio foliage, to three commercial control products derived fromBacillus thuringiensis (Bt), was evaluated in the laboratory. Both Dipel DF and Delfin WG were highly effective against 1 st and 2 nd instar larvae at relatively low concentrations (LC50: 0.01–0.04%), whereas mortality of the 3 rd instar larvae reached 50% only at a Dipel DF concentration of 0.2%. The third formulation, Foray 48B, was significantly less toxic toward all tested larval instars. The susceptibility ofT. solitaria toBr decreased progressively between molts, and then increased steeply during the first days following each molt. Use of the film-forming polymer Nu-Film as a wetting agent and spreader-sticker for the commercial formulation of Dipel DF improvedBt adhesion to extremely hydrophobic youngPistacia foliage, and significantly increasedBt persistence under ambient conditions. Addition of Nu-Film improved the leaf surface coverage and extended the duration of rainproofing.  相似文献   
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86.
Potentilla alba L. is a valuable medicinal plant widely used in folk and traditional medicine and particularly promising in complex treatment of thyroid pathology. Natural resources of this species are insufficient due to ever-growing use in contemporary medicine. Comprehensive investigations of different P. alba populations are essential for the successful extension of P. alba plantings. Aiming for a better understanding of karyotype structure, chromosome behaviour in meiosis and developing new diagnostic characters, we performed molecular cytogenetic characterization and leaf structure and ultrastructure analyses of two introduced P. alba samples originating from different habitats. Based on chromosome morphology, distribution of 45S/5S rDNA and DAPI-banding patterns, all chromosomes in the karyotypes were identified and the P. alba chromosomal idiogram was constructed. Our findings confirmed P. alba karyotype stability and also revealed several diagnostic characters of this species: the features of cells of upper and lower leaf epidermis, the presence of calcium oxalate druses and three types of leaf indumentum, essential for evaluation of genetic diversity in different populations, validation of raw materials and further selection progress. The meiotic abnormalities were detected probably related to low pollen activity and indicated the advantages of vegetative propagation in the development of a P. alba plantation system.  相似文献   
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89.
In aquaculture, nutrition and supplemented diets have been shown to affect broodstock reproductive performance. In this study, we investigated the effects of dietary supplementation with Cymbopogon flexuosus essential oil (CFEO) microcapsules on reproductive-related parameters in silver catfish (Rhamdia quelen) male broodfish. Adult male broodstocks were separated into three groups according to the concentrations of supplemented CFEO (0.0?=?control; 1.0 or 3.0 mL per kg of diet). After 20 days under experimental conditions, the animals were euthanized and the gonads were harvested for gonadosomatic index, sperm analysis, oxidative stress, and histopathology; testosterone levels were measured in the plasma; gene expression of prl, smtl, pomca, and pomcb was assessed in the pituitary gland by real-time PCR. The results showed no alterations on reproductive parameters in R. quelen males treated with Cymbopogon flexuosus essential oil compared to the control-diet animals. In conclusion, CFEO microcapsules supplied for 20 days in the concentrations of 1.00 or 3.00 mL per kilogram of diet did not affect the reproduction criteria evaluated in this study in male silver catfish.  相似文献   
90.
Fresh Atlantic cod is available in large amounts in Norway during the first 5 months of the year. Live-storage of cod may extend the marketing period of fresh cod products throughout the year. In addition, this concept makes pre-rigor processing possible. The main problem of keeping wild cod in captivity is that it does not easily accept formulated dry feed. The purpose of this study was, therefore, to investigate how long-term live-storage of mature Atlantic cod in the absence of feed (79 days) affects the onset and development of rigor mortis, as well as fillet quality by assessing hardness and water-holding capacity of the muscle, pH, protein, and water content. The results showed that starvation of Atlantic cod for 23 days reduces the pre-rigor time from 29 to 17 h. Further starvation did not decrease this period significantly, suggesting that live-stored cod deprived of feed for 79 days may still be industrially processed before the onset of rigor mortis. More than 51 days of starvation reduced protein concentration and increased water content of the muscle. After 51 days, the muscle texture was softer than in fish starved for a shorter period.  相似文献   
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