Influence of seminal plasma during different stages of bovine sperm cryopreservation     

Gabriela Bertaiolli Zoca  Eneiva Carla Carvalho Celeghini  Guilherme Pugliesi  Carla Patricia Teodoro de Carvalho  Mayra Elena Ortiz D'Avila Assumpção  Adriano Felipe Perez Siqueira  Leticia Zoccolaro Oliveira  Renata Lançoni  Rubens Paes de Arruda 《Reproduction in domestic animals》2021,56(6):872-883

This study aimed to evaluate the effect of seminal plasma on bovine sperm cryopreservation and to assess the integrity of plasma and acrosomal membranes, mitochondrial potential, remodelling of F-actin cytoskeleton and sperm chromatin fragmentation during the cooling, equilibrium and freezing/thawing stages. Six ejaculates collected from seven Nelore bulls (n = 42) were used in this study. Each ejaculate was divided into two aliquots (with seminal plasma = SP group; without seminal plasma = NSP group) and packed to a final concentration of 50 × 10⁶ sperm per straw. Statistical analyses were performed using SAS software (version 9.3), and p ≤ .05 was considered significant. A time effect was observed for all sperm characteristics (p < .05), except for chromatin fragmentation (p > .05). The presence of seminal plasma better preserved the acrosomal integrity (SP = 75.2% and NSP = 71.7%; p < .05) and also provided lower F-actin remodelling during cryopreservation process (SP = 29.9% and NSP = 32.4%; p < .05). Regarding to the cryopreservation stages, it was observed that cooling step induced higher remodelling of F-actin than the equilibrium and freezing/thawing stages (56.3%, 32.2% and 23.9%, respectively; p < .05). The equilibrium step had minor influence on overall sperm characteristics while the freezing/thawing stage was responsible for the highest percentage of damage in plasma membrane (−65.2%), acrosomal membrane (−34.0%) and mitochondrial potential (−48.1%). On the other hand, none of the cryopreservation stages affected chromatin integrity. It was concluded that the presence of seminal plasma provides increased acrosomal integrity and reduced remodelling of F-actin cytoskeleton. Higher F-actin remodelling is observed after the cooling step while the freezing/thawing step is most damaging to sperm membranes and mitochondrial potential during bovine sperm cryopreservation.  相似文献   

Degradation of the coffee flavor compound furfuryl mercaptan in model Fenton-type reaction systems     

Blank I  Pascual EC  Devaud S  Fay LB  Stadler RH  Yeretzian C  Goodman BA 《Journal of agricultural and food chemistry》2002,50(8):2356-2364

The stability of the coffee flavor compound furfuryl mercaptan has been investigated in aqueous solutions under Fenton-type reaction conditions. The impact of hydrogen peroxide, iron, ascorbic acid, and ethylenediaminetetraacetic acid was studied in various combinations of reagents and temperature. Furfuryl mercaptan reacts readily under Fenton-type reaction conditions, leading to up to 90% degradation within 1 h at 37 degrees C. The losses were lower when one or more of the reagents was omitted or the temperature decreased to 22 degrees C. Volatile reaction products identified were mainly dimers of furfuryl mercaptan, difurfuryl disulfide being the major compound. In addition, a large number of nonvolatile compounds was observed with molecular masses in the range of 92-510 Da. The formation of hydroxyl and carbon-centered radicals was indicated by electron paramagnetic resonance spectra using alpha-(4-pyridyl-1-oxide)-N-tert-butylnitrone or 5-(diethoxyphosphoryl)-5-methyl-1-pyrroline-N-oxide as spin traps. Whereas *OH was generated by Fenton-type reactions, the C-centered radical is probably a secondary product of the reaction of *OH with various organic molecules, the reaction with furfuryl mercaptan appearing to be the most important. No evidence for S-centered radicals was seen in the spin-trapping experiments, but a sulfur-containing radical was detected when measurements were made at 77 K in the absence of spin traps.  相似文献   

Alkylpyridiniums. 2. Isolation and quantification in roasted and ground coffees     

Stadler RH  Varga N  Milo C  Schilter B  Vera FA  Welti DH 《Journal of agricultural and food chemistry》2002,50(5):1200-1206

Recent model studies on trigonelline decomposition have identified nonvolatile alkylpyridiniums as major reaction products under certain physicochemical conditions. The quaternary base 1-methylpyridinium was isolated from roasted and ground coffee and purified by ion exchange and thin-layer chromatography. The compound was characterized by nuclear magnetic resonance spectroscopy ((1)H, (13)C) and mass spectrometry techniques. A liquid chromatography-electrospray ionization tandem mass spectrometry method was developed to quantify the alkaloid in coffee by isotope dilution mass spectrometry. The formation of alkylpyridiniums is positively correlated to the roasting degree in arabica coffee, and highest levels of 1-methylpyridinium, reaching up to 0.25% on a per weight basis, were found in dark roasted coffee beans. Analyses of coffee extracts also showed the presence of dimethylpyridinium, at concentrations ranging from 5 to 25 mg/kg. This is the first report on the isolation and quantification of alkylpyridiniums in coffee. These compounds, described here in detail for the first time, may have an impact on the flavor/aroma profile of coffee directly (e.g., bitterness), or indirectly as precursors, and potentially open new avenues in the flavor/aroma modulation of coffee.  相似文献   

Analysis of matrix-bound nitrofuran residues in worldwide-originated honeys by isotope dilution high-performance liquid chromatography-tandem mass spectrometry   总被引：1，自引：0，他引：1  

Khong SP  Gremaud E  Richoz J  Delatour T  Guy PA  Stadler RH  Mottier P 《Journal of agricultural and food chemistry》2004,52(17):5309-5315

A sensitive and selective isotope dilution liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESIMS/MS) method is presented for the simultaneous analysis of the metabolites of four nitrofuran veterinary drugs, that is, furazolidone, furaltadone, nitrofurantoin, and nitrofurazone, in honey samples. The method entails a combined hydrolysis of protein-bound drug metabolites and derivatization of the resulting metabolites with 2-nitrobenzaldehyde (NBA) during an overnight incubation, followed by a liquid-liquid extraction and a cleanup on a polymeric solid-phase extraction cartridge. Mass spectral acquisition is carried out in the positive ion mode by applying multiple reaction monitoring (MRM) of three diagnostic transition reactions for each analyte under survey. A reliable quantification is obtained by the use of one deuterated analogue per analyte (NBA-d(4) derivative). The method has been validated in honey according to the European Union criteria for the analysis of veterinary drug residues in food. Expressed in underivatized nitrofuran metabolite concentrations, the decision limits (CCalpha) ranged within 0.07-0.46 microg/kg, and the detection capabilities (CCbeta) were within 0.12-0.56 microg/kg. The method has been successfully applied in a survey of honeys of various geographical origins, showing that furazolidone is the main nitrofuran antibiotic administered to treat bacterial diseases of bees.  相似文献   

High‐field (3 Tesla) MRI of the navicular apparatus of sound horses shows good agreement to histopathology     

Lena K. Kottmeier  Frauke Seehusen  Martin Helweg  Karl Rohn  Peter Stadler  Maren Hellige 《Veterinary radiology & ultrasound》2020,61(1):48-57

Magnetic resonance imaging and the correlation to histopathological findings of the equine palmar foot of lame horses have been described previously, using 0.27 and 1.5 T systems. Compared to these, 3 T systems provide superior spatial resolution and imaging contrast. The aim of our prospective anatomic study was to characterize the imaging anatomy of the navicular region on 3 T MRI in comparison to histopathological findings. We hypothesized that 3 T MRI allows a good visualization of the entire navicular apparatus and reliable measurements of navicular cartilage and cortical bone thickness. Twenty front feet of sound horses were examined using a 3 T MRI system. For histopathological examination, sagittal tissue sections of the navicular bones and adjacent ligaments were prepared. Alterations in magnetic resonance signal were graded for each region and compared to corresponding histological slices. Overall, there was good visualization of the anatomical detail and a very good agreement between MRI and histology for compact bone and spongiosa, good agreement for the fibrocartilage and the distal sesamoidean impar ligament, but only moderate agreement for the hyaline cartilage and the collateral sesamoidean ligament. A comparative measurement of cartilage and cortical bone thickness on magnetic resonance images and histological sections was performed. In MRI, the hyaline cartilage of the articular surface appeared significantly thinner and the fibrocartilage of the flexor surface appeared significantly thicker compared to histology. Findings indicated that MRI at a field strength of 3 T allows reliable depiction of anatomic details of the navicular apparatus.  相似文献   

Milkfish seedling survival in raceways of freshwater recirculating systems     

Jose A. Carreon  Liberato V. Laureta  Firmo A. Estocapio  Teodoro U. Abalos 《Aquaculture (Amsterdam, Netherlands)》1984,36(3):257-272

Wooden and concrete raceways in closed recirculating freshwater systems were tested for rearing milkfish (Chanos chanos) fry up to 2 to 3 cm total length. Artificially produced detritus and natural plankton were tested separately as food. Growth, health and survival were good for larvae supplied natural plankton, but poor for those reared on detritus.Precipitation, temperature and intensity of solar insolation had no adverse effects on the production of natural plankton cultivated in the outdoor closed systems. Fish growth was better in experiments in June–July than in August and September.The concrete raceways, 86 m³ at fixed water depth, could produce an estimated 405 000 viable seedlings of 2 to 3 cm length in a 10-month period. Average survival was 70%. Water flow in the raceways was 10 cm/s.  相似文献   

Permeability of cork to gases     

Faria DP  Fonseca AL  Pereira H  Teodoro OM 《Journal of agricultural and food chemistry》2011,59(8):3590-3597

The permeability of gases through uncompressed cork was investigated. More than 100 samples were assessed from different plank qualities to provide a picture of the permeability distribution. A novel technique based on a mass spectrometer leak detector was used to directly measure the helium flow through the central area of small disks 10 mm in diameter and 2 mm thick. The permeability for nitrogen, oxygen, and other gases was measured by the pressure rise technique. Boiled and nonboiled cork samples from different sections were evaluated. An asymmetric frequency distribution ranging 3 orders of magnitude (roughly from 1 to 1000 μmol/(cm·atm·day)) for selected samples without macroscopic defects was found, having a peak below 100 μmol/(cm·atm·day). Correlation was found between density and permeability: higher density samples tend to show lower permeability. However, boiled cork showed a mean lower permeability despite having a lower density. The transport mechanism of gases through cork was also examined. Calculations suggest that gases permeate uncompressed cork mainly through small channels between cells under a molecular flow regime. The diameter of such channels was estimated to be in the range of 100 nm, in agreement with the plasmodesmata size in the cork cell walls.  相似文献   

Baseflow control on sediment flux connectivity: Insights from a nested catchment study in Central Mexico   总被引：1，自引：0，他引：1  

Clément Duvert  Nicolas Gratiot  Roberto Anguiano-Valencia  Julien Némery  Manuel E. Mendoza  Teodoro Carlón-Allende  Christian Prat  Michel Esteves 《CATENA》2011

In order to assess the extent of sediment connectivity between uplands and lowlands and to quantify the processes of in-channel deposition and remobilization, measurements of suspended sediment fluxes were conducted in a nested rural catchment of the Mexican Volcanic Belt. Data were collected over one year at three upland sites (3 to 12 km²) and two downstream stations (390–630 km²). Our results show that a structural discontinuity in the catchment (i.e. abrupt slope decrease at the junction between piedmonts and the alluvial plain from 2 to 10% to < 0.1%) could be compensated by functional continuity during floods. Direct conveyance of fine sediment to the outlet occurred when a high stream transport capacity was reached. Erosion of the streambed was observed on various occasions and accounted for up to 50% of the flux leaving the catchment during one event. Conversely, temporary in-channel storage was apparent on other occasions, amounting to up to 52% of the flux recorded upstream during one storm. These two distinct behaviours were approximately equally distributed along the rainy season and strongly driven by the extent of coupling between surface and subsurface water. This work indeed highlights the role of baseflow spatial variations in determining the extent of lowland sediment conveyance. Riverbed erosional processes occurred when large differences in pre-event baseflow values (i.e. at least a twofold longitudinal increase) were observed between the 5-km distant lowland stations. Our findings outline the importance of systematically taking into consideration the baseflow parameter in research focusing on fine sediment transport across scales.  相似文献   

Effect of dietary iron content on hematologic and other measures of iron adequacy in dogs     

B R Weeks  J E Smith  C K Stadler 《Journal of the American Veterinary Medical Association》1990,196(5):749-753

Eighteen 9- to 10-week old Beagles were fed casein-based diets (4,710 kcal of metabolizable energy/kg of body weight) containing either 12, 80, or 160 mg of iron/kg of diet. Growth and feed consumption were monitored throughout the 47-day study. Hematocrit (Hct), hemoglobin (Hb) concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), RBC numbers, erythrocyte protoporphyrin (EP) concentration, serum iron concentration, serum total iron-binding capacity (TIBC), and serum ferritin concentration were determined weekly. Growth rate and feed efficiency were not significantly influenced by dietary iron content. At 14 days, Hb concentration, Hct, MCV, MCH, RBC numbers, and serum iron concentration were significantly (P less than 0.05) lower in dogs fed the 12 mg/kg diet, and remained significantly low for the remainder of the study. Erythrocyte protoporphyrin concentration increased significantly (P less than 0.05) by 14 days in dogs fed the basal diet, and remained significantly high relative to that in dogs of the other dietary groups for the remainder of the study. Serum ferritin concentration decreased in dogs of the group fed the basal diet, with a significant (P less than 0.05) difference beyond day 42. Differences in Hct, MCH, MCV, or hemoglobin, serum iron, serum ferritin, or EP concentration were not found between groups fed 80 and 160 mg of iron/kg of diet. Liver nonheme iron content was significantly (P less than 0.05) affected by dietary iron content.  相似文献   

Prognosis of aboveground woody biomass in a central Brazilian Cerrado monitored for 27 years after the implementation of management systems     

de Azevedo  Gileno Brito  Rezende  Alba Valéria  Azevedo  Glauce Taís de Oliveira Sousa  Miguel  Eder Pereira  Aquino  Fabiana de Gois  Teodoro  Larissa Pereira Ribeiro  Teodoro  Paulo Eduardo 《European Journal of Forest Research》2022,141(1):1-15

Accurate estimation of biomass in natural vegetation sites remains a challenge. Modeling biomass growth and production in Cerrado areas is crucial to understanding the vegetation succession process, especially regarding the changes in biomass accumulation over time. Thus, our objective was to model the growth and production of woody aboveground biomass (living and total) in a cerrado stricto sensu monitored for 27 years after implementing management systems. As expected, the basal area (with a diameter taken at 30 cm from the ground level) is the most important predictor variable and showed a higher correlation with the biomass stocks and allowed accurate and consistent estimates of these accumulated stocks over time. Future estimates of biomass production, generated from growth models that estimate production as a function of parameters observed at previous ages, indicate that maximum stocks of living (25.86?±?0.15 Mg ha^?1 [mean?±?standard deviation]) and total aboveground biomass (26.11?±?0.15 Mg ha^?1) are expected for a period between 28 and 30 years after the implementation of the management systems, with maximum mean annual increment between 23 and 27 years. Furthermore, the systems of equations obtained simulated reductions up to 30% of biomass after the occurrence of a forest fire at 23 years. Thus, our study can be useful for the decision-making process and developing public policies and strategies for managing and conserving natural resources in the Cerrado biome.

  相似文献