An improved vitrification protocol for equine immature oocytes,resulting in a first live foal          下载免费PDF全文

N. Ortiz‐Escribano  O. Bogado Pascottini  H. Woelders  L. Vandenberghe  C. De Schauwer  J. Govaere  E. Van den Abbeel  T. Vullers  C. Ververs  K. Roels  M. Van De Velde  A. Van Soom  K. Smits 《Equine veterinary journal》2018,50(3):391-397

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A Comparison of Two Herbaceous Cover Sampling Methods to Assess Ecosystem Services in High-Shrub Rangelands: Photography-Based Grid Point Intercept (GPI) Versus Quadrat Sampling     

Kristin B. Hulvey  Katherine Thomas  Eric Thacker 《Herpetological Monographs》2018,40(5):152-159

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Equine foetal gender determination in mid‐ to late gestational mares: A practical inquiry          下载免费PDF全文

Margot Van de Velde  Kim Roels  Cyrillus Ververs  Ilse Gerits  Jan Govaere 《Reproduction in domestic animals》2018,53(5):1027-1032

In recent years, the interest in equine foetal gender determination (FGD) during gestation increased remarkably. Ultrasonographic FGD can be performed in two different periods during gestation. The earliest examination can take place at a gestational age of 60–70 days, whereby the genital tubercle is used to differentiate between male and female foeti. The time window of the second approach is wider (120–210 days), and there are more characteristics to take into consideration. In this article, the feasibility and accuracy of ultrasonographic FGD in mid‐ to late gestation are evaluated. One hundred twenty‐one mares from different breeds with a pregnancy stage between 120 and 270 days were examined once, using B‐scale ultrasonography (Esaote MyLab™ClassC). None of the mares were sedated nor shaved, and the procedure was completed within 15 min. Diagnosis was firstly based on the gonads. The final judgement was made based on all visible foetal reproductive organs. In three cases with a pregnancy stage beyond 257 days, FGD was not possible. All of the examined mares in which a FGD could be performed gave birth to a healthy foal. In 98% of the examinations (116/118), the diagnosis was correctly made. In both cases of misdiagnosis, only one characteristic was seen during the procedure and wrongly interpreted. Beyond 210 days of pregnancy, the extremities can preclude a good visualization of the inguinal region. In conclusion, equine FGD in mid‐ to late gestation is an accessible and accurate technique, although a good ultrasound device is a prerequisite and experience and expertise is necessary.  相似文献   

Effect of weaning age and the use of different sized Artemia nauplii as first feed for jade perch Scortum barcoo     

Stijn Van Hoestenberghe  Mathieu Wille  Evelien De Swaef  Bruno M. Goddeeris  Nancy Nevejan 《Aquaculture International》2015,23(6):1539-1552

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The delay of flowering time in almond: a review of the combined effect of adaptation,mutation and breeding     

Pedro Martínez-Gómez  Angela S. Prudencio  Thomas M. Gradziel  Federico Dicenta 《Euphytica》2017,213(8):197

Regulation of flowering time in almond, as in other Prunus species, is a complex process involving both chill and heat requirements. Following exposure to appropriate consecutive periods of cold and warm temperatures, the buds break dormancy and sprout or flower depending on bud type. To maximize flowering and subsequent vegetative growth and fruit set, chilling and ensuing warm temperature requirements have to be fully satisfied. Because of its potential for very early flowering, flowering time in almond is a major determinant of its adaptation to new environments. In colder regions, Late-flowering is often necessary to avoid frost damage during and just after flowering. Consequently, the selection of delayed flowering times remains an important objective in almond improvement programs. Flowering time is considered a quantitative though highly heritable trait. In addition, a dominant gene (Late flowering, Lb), originally identified in a spontaneous mutation of the Californian almond cultivar ‘Nonpareil’, was also described. The objective of this review is a comparative analysis of the effects of regional adaptation, breeding and mutation on the delay of flowering time in new almond cultivars. Findings indicate that the adaptation of almonds from the Mediterranean basin to colder regions in Northern Europe and America has been mainly achieved through delayed flowering. These adapted late-flowering cultivars have usually been developed by selecting desired quantitative genes within each regional germplasm. Additional progress thus appears achievable with a more comprehensive understanding of the quantitative and qualitative genetics controlling this trait. The use of molecular markers for the early selection of genes conferring late flowering, including both spontaneous mutations as well as unique regional germplasm, should allow development of even later cultivars including ultra-late cultivars flowering as into April.  相似文献   

Comparison of mitochondrial gene expression and polysome loading in different tobacco tissues     

Muhammad Waqar Hameed  Ilona Juszczak  Ralph Bock  Joost Thomas van Dongen 《Plant methods》2017,13(1):112

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Impact of traditional soil burning (guie) on Planosol properties and land‐use intensification in south‐western Ethiopia          下载免费PDF全文

A. Verdoodt  M. Dumon  J. Deckers  E. Van Ranst 《Soil Use and Management》2015,31(2):330-336

In the Gilgel Gibe catchment in Ethiopia, local farmers intensify land use on Planosols by adjusting a traditional soil burning practice known as guie. The burning practice used to be applied in a cycle of shifting cultivation. However, more recently, farmers burn small plots to make fertile seedbeds for Eucalyptus seedlings in the first year before these trees are transplanted to larger plots. The purpose of this research was to assess the physico‐chemical properties of Planosols that have been subjected to burning over the last 10 yrs and evaluate the contribution of guie to land‐use intensification of these soils. Transect studies and interviews of local farmers, followed by chemical, physical and micromorphological analyses of samples from selected plots were used to compare the soil properties of recently (0–2 yrs) and formerly (3–10 yrs) burnt Planosols with those of unburnt Planosols. The analytical results show that the burning practice improved nutrient availability in the first 2 yrs after guie. Increased amounts of exchangeable aluminium (Al) were reported in the long term. Charge fingerprints illustrate that the nutrient‐buffering capacity of the soil was high shortly after the practice but subsequently decreased with time. Given the population pressure on the formerly extensively used Planosols, it is argued that the current application of guie on small, localized plots for raising Eucalyptus seedlings is well adapted to the local socio‐economic context and promotes land‐use intensification on the Planosols. The increased exchangeable Al content of former Eucalyptus seedbeds merits further in‐depth research into the biophysical sustainability of the burning practice.  相似文献   

Induced expression of selected plant defence related genes in pot azalea, <Emphasis Type="Italic">Rhododendron simsii</Emphasis> hybrid     

Gil Luypaert  Johan Witters  Johan Van Huylenbroeck  Patrick De Clercq  Jan De Riek  Ellen De Keyser 《Euphytica》2017,213(10):227

A set of putative marker genes to study plant defense responses against Polyphagotarsonemus latus, a key pest in the production of Rhododendron simsii hybrids, was selected and validated. Genes belonged to the biosynthetic pathway of phytohormones jasmonic acid (JA) (RsLOX, RsAOS, RsAOC, RsOPR3 and RsJMT) and salicylic acid (SA) (RsPAL and RsICS). Furthermore, RsPPO, a putative marker gene for oxidative stress response was successfully cloned from R. simsii. A CTAB-based extraction protocol was optimized to assure excellent RNA quality for subsequent RT-qPCR analysis. The RT-qPCR protocol was extensively tested and RsRG7 and RsRG14 were selected as reference genes from a geNorm pilot study. Validation of the marker genes was done after application with elicitors [methyl jasmonate (MeJA), coronatine, β-aminobutyric acid and acibenzolar-Smethyl] or wounding. Both 100 μM MeJA and 0.1 μM coronatine had a significant effect on the expression of all marker genes. Foliar application of MeJA on the shoots resulted in a significantly earlier response when compared to root application and subsequent sampling of the shoots. Expression patterns after MeJA treatment were generally the same in six R. simsii genotypes: ‘Nordlicht’, ‘Elien’, ‘Aiko Pink’, ‘Michelle Marie’, ‘Mevrouw Gerard Kint’ and ‘Sachsenstern’. Wounding resulted in the same expression patterns as MeJA treatment except for RsJMT. None of the genotypes showed a significant induction of the latter gene 6 h upon wounding. Findings of these experiments indicated that the tolerant genotype ‘Elien’ has low basal expression levels of RsPPO. This might be the first step towards the breeding of mite-tolerant genotypes.  相似文献   

A multi-method approach to delineate and validate migratory corridors     

Monica L. Bond  Curtis M. Bradley  Christian Kiffner  Thomas A. Morrison  Derek E. Lee 《Landscape Ecology》2017,32(8):1705-1721

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Journal of Horticultural Science and Biotechnology – Journal Prize 2019     

Brian Thomas  Geoffrey R. Dixon  Ms Jennifer Stokes 《The Journal of Horticultural Science and Biotechnology》2019,94(5)

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