Tricyclic Guanidine Alkaloids from the Marine Sponge Acanthella cavernosa that Stabilize the Tumor Suppressor PDCD4     

Tanja Grkovic  Johanna S. Blees  Magdalena M. Bayer  Nancy H. Colburn  Cheryl L. Thomas  Curtis J. Henrich  Megan L. Peach  James B. McMahon  Tobias Schmid  Kirk R. Gustafson 《Marine drugs》2014,12(8):4593-4601

A cell-based high-throughput screen that assessed the cellular stability of a tumor suppressor protein PDCD4 (Programmed cell death 4) was used to identify a new guanidine-containing marine alkaloid mirabilin K (3), as well as the known compounds mirabilin G (1) and netamine M (2). The structures of these tricyclic guanidine alkaloids were established from extensive spectroscopic analyses. Compounds 1 and 2 inhibited cellular degradation of PDCD4 with EC₅₀ values of 1.8 μg/mL and 2.8 μg/mL, respectively. Mirabilin G (1) and netamine M (2) are the first marine natural products reported to stabilize PDCD4 under tumor promoting conditions.  相似文献   

The use of historical collections to estimate population trends: A case study using Swedish longhorn beetles (Coleoptera: Cerambycidae)     

Tobias Jeppsson  Anders Lindhe  Pär Forslund 《Biological conservation》2010,143(9):1940-1950

Long term data to estimate population trends among species are generally lacking. However, Natural History Collections (NHCs) can provide such information, but may suffer from biases due to varying sampling effort. To analyze population trends and range-abundance dynamics of Swedish longhorn beetles (Coleoptera: Cerambycidae), we used collections of 108 species stretching over 100 years. We controlled for varying sampling effort by using the total number of database records as a reference for non-red-listed species. Because the general frequency of red-listed species increased over time, a separate estimate of sampling effort was used for that group. We observed large interspecific variation in population changes, from declines of 60% to several hundred percent increases. Most species showed stable or increasing ranges, whereas few seemed to decline in range. Among increasing species, rare species seemed to expand their range more than common species did, but this pattern was not observed in declining species. Historically, rare species did not seem to be at larger risk of local extinction, and population declines were mostly due to lower population density and not loss of sub-populations. We also evaluated the species’ declines under IUCN red-list criterion A, and four currently not red-listed species meet the suggested threshold for Near Threatened (NT). The results also suggested that species’ declines may be overlooked if estimated only from changes in species range.  相似文献   

European Bison habitat in the Carpathian Mountains     

Tobias Kuemmerle  Kajetan Perzanowski  Oleh Chaskovskyy 《Biological conservation》2010,143(4):908-916

European Bison (Bison bonasus) barely escaped extinction in the early 20th century and now only occur in small isolated herds scattered across Central and Eastern Europe. The species’ survival in the wild depends on identifying suitable habitat for establishing bison metapopulations via reintroductions of new herds. We assessed European Bison habitat across the Carpathian Mountains, a stronghold of European Bison and one of the only places where a viable bison metapopulation may be possible. We used maximum entropy models to analyze herd range maps and habitat use data from radio-collared bison to identify key habitat variables and map European Bison habitat across the entire Carpathian ecoregion (210,000 km²). Forest cover (primarily core and perforated forests) and variables linked to human disturbance best predict bison habitat suitability. Bison show no clear preference for particular forest types but prefer managed grasslands over fallow and abandoned fields. Several large, suitable, but currently unoccupied habitat patches exist, particularly in the eastern Carpathians. This available suitable habitat suggests that European Bison have an opportunity to establish a viable Carpathian metapopulation, especially if recent trends of declining human pressure and reforestation of abandoned farmland continue. Our results also confirm the suitability of a proposed Romanian reintroduction site. Establishing the first European Bison metapopulation would be a milestone in efforts to conserve this species in the wild and demonstrate a significant and hopeful step towards conserving large grazers and their ecological roles in human-dominated landscapes across the globe.  相似文献   

Modelling experts’ judgments on soil compaction to derive decision rules for soil protection—A case study from Switzerland     

Silvia Tobias  Olaf Tietje 《Soil & Tillage Research》2007,92(1-2):129-143

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Interlinked fast and slow positive feedback loops drive reliable cell decisions     

Brandman O  Ferrell JE  Li R  Meyer T 《Science (New York, N.Y.)》2005,310(5747):496-498

Positive feedback is a ubiquitous signal transduction motif that allows systems to convert graded inputs into decisive, all-or-none outputs. Here we investigate why the positive feedback switches that regulate polarization of budding yeast, calcium signaling, Xenopus oocyte maturation, and various other processes use multiple interlinked loops rather than single positive feedback loops. Mathematical simulations revealed that linking fast and slow positive feedback loops creates a "dual-time" switch that is both rapidly inducible and resistant to noise in the upstream signaling system.  相似文献   

Temperature Dependence of Wing Abnormality in Tribolium confusum     

Slater JV  Rescigno A  Amer NM  Tobias CA 《Science (New York, N.Y.)》1963,140(3565):408-409

When Tribolium confusum pupae were exposed continuously to temperatures from 10 degrees C to 40 degrees C, the adults which finally emerged exhibited wing abnormalities typical of those obtained with x-irradiation. The data indicate the implication of two processes. Two mathematical models are suggested to account for this phenomenon.  相似文献   

Endocranial features of Australopithecus africanus revealed by 2- and 3-D computed tomography   总被引：4，自引：0，他引：4  

G C Conroy  M W Vannier  P V Tobias 《Science (New York, N.Y.)》1990,247(4944):838-841

The earliest hominid from South Africa, Australopithecus africanus, is known from only six specimens in which accurate assessment of endocranial capacity and cranial venous outflow pattern can be obtained. This places a severe limit on a number of hypotheses concerning early hominid evolution, particularly those involving brain-body size relationships and adaptations of the circulatory system to evolving upright posture. Advances in high-resolution two- and three-dimensional computed tomography (CT) now allow the inclusion of another important specimen to this list, MLD 37/38 from Makapansgat. A new computer imaging technique is described that "reconstructs" the missing portions of the endocranial cavity in order to determine endocranial capacity. In addition, CT evaluation allows assessment of cranial venous outflow pattern even in cases where the endocranial cavity is completely filled with stone matrix. Results show that endocranial capacity in this specimen is less than originally proposed and also support the view that gracile and robust australopithecines evolved different cranial venous outflow patterns in response to upright postures.  相似文献   

Axonal neuregulin-1 regulates myelin sheath thickness     

Michailov GV  Sereda MW  Brinkmann BG  Fischer TM  Haug B  Birchmeier C  Role L  Lai C  Schwab MH  Nave KA 《Science (New York, N.Y.)》2004,304(5671):700-703

In the nervous system of vertebrates, myelination is essential for rapid and accurate impulse conduction. Myelin thickness depends on axon fiber size. We use mutant and transgenic mouse lines to show that axonal Neuregulin-1 (Nrg1) signals information about axon size to Schwann cells. Reduced Nrg1 expression causes hypomyelination and reduced nerve conduction velocity. Neuronal overexpression of Nrg1 induces hypermyelination and demonstrates that Nrg1 type III is the responsible isoform. We suggest a model by which myelin-forming Schwann cells integrate axonal Nrg1 signals as a biochemical measure of axon size.  相似文献   

Response: faunal evidence and sterkfontein member 2 foot bones of early hominid     

Tobias PV  Clarke RJ 《Science (New York, N.Y.)》1996,271(5253):1301b-1302b

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Dynamic visualization of thrombopoiesis within bone marrow     

Junt T  Schulze H  Chen Z  Massberg S  Goerge T  Krueger A  Wagner DD  Graf T  Italiano JE  Shivdasani RA  von Andrian UH 《Science (New York, N.Y.)》2007,317(5845):1767-1770

Platelets are generated from megakaryocytes (MKs) in mammalian bone marrow (BM) by mechanisms that remain poorly understood. Here we describe the use of multiphoton intravital microscopy in intact BM to visualize platelet generation in mice. MKs were observed as sessile cells that extended dynamic proplatelet-like protrusions into microvessels. These intravascular extensions appeared to be sheared from their transendothelial stems by flowing blood, resulting in the appearance of proplatelets in peripheral blood. In vitro, proplatelet production from differentiating MKs was enhanced by fluid shear. These results confirm the concept of proplatelet formation in vivo and are consistent with the possibility that blood flow-induced hydrodynamic shear stress is a biophysical determinant of thrombopoiesis.  相似文献