Association of intrauterine presence of Lactobacillus spp. with inflammation and pathogenic bacteria in the uterus in postpartum dairy cows     

Xinyue WU  Go KITAHARA  Tetsuya SUENAGA  Kanami NARAMOTO  Satoshi SEKIGUCHI  Yoshitaka GOTO  Takeshi OSAWA 《The Journal of reproduction and development》2021,67(6):340

The aim of this study was to clarify the influence of Lactobacillus spp. on the degree of endometrial inflammation in the postpartum period and the relationship between Lactobacillus spp. and pathogenic bacteria in the endometrium of postpartum dairy cows. Endometrial samples were collected from 41 Holstein-Friesian cows at 4 and 8 weeks postpartum using cytobrushes for polymorphonuclear neutrophil (PMN) count and bacterial culture to isolate Lactobacillus spp., Escherichiacoli, and Trueperella pyogenes. The 4-week samples were divided into four groups (E+L+), (E+L−), (E−L+), (E−L−) according to whether endometritis was diagnosed (E+) and Lactobacillus spp. was isolated (L+). The diagnostic criterion for cytological endometritis was > 18% PMN. The average PMN% in the E+L+ group was lower than that in the E+L-group (P < 0.05) at 8 weeks postpartum. There were no significant correlations between the number of colonies of Lactobacillus spp. and E. coli or between that of Lactobacillus spp. and T. pyogenes. Lactobacillus spp. could reduce PMN% in dairy cows with endometritis during the puerperal period. In conclusion, the intrauterine presence of Lactobacillus spp. may have a positive effect on uterine involution in postpartum dairy cows.  相似文献   

IMAGING DIAGNOSIS—NEURONAL CEROID LIPOFUSCINOSIS WITH A CHRONIC SUBDURAL HEMATOMA     

MIDORI GOTO ASAKAWA  EDWARD MACKILLOP  NATASHA J. OLBY  IAN D. ROBERTSON  JOHN M. CULLEN 《Veterinary radiology & ultrasound》2010,51(2):155-158

A subdural hematoma was found to accompany neuronal ceroid lipofuscinosis in an 11‐month‐old Dachshund. Results from clinical, magnetic resonance (MR) imaging, histopathologic, ultrastructural, and molecular assessments are described. The dog had a 3‐month history of progressive neurologic signs. In MR images, there was severe asymmetric cerebral atrophy with a subdural hematoma. Histopathologically, there was autofluorescent, periodic acid–Schiff‐positive lipopigment in neurons and transmission electron microscopy confirmed a typical curvilinear profile of the storage bodies. We hypothesize that rapid brain atrophy contributed to the subdural hematoma formation, a complication not described previously in dogs with neuronal lipofuscinosis.  相似文献   

Congenital malformations of the external and middle ear accompanied by temporal bone anomaly in a calf     

Naoyuki AIHARA  Anna MOMOKI  Nanase HATTORI  Hironobu MURAKAMI  Motoharu OISHI  Yoshiaki GOTO  Reiichiro SATO  Makoto NAGAI  Kazutaka YAMADA  Junichi KAMIIE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(2):302

A 7-month-old female Holstein calf presented with bilateral microtia and absent external acoustic meatus. The real-time polymerase chain reaction test was negative for bovine viral diarrhea virus and bovine leukemia virus. The calf’s dam had a normal reproductive history. Computed tomography confirmed bilateral atresia of external auditory canals, aplasia of tympanic cavities and the ossicular chain, and temporomandibular joint abnormality. Necropsy revealed a severe malformation of the temporal bone. In the tympanic region, the external acoustic pore, tympanic bulla, and muscular process were absent bilaterally. The bilateral inner ear structure was normal. Based on these findings, we diagnosed the present case as congenital malformations of the external and middle ear accompanied by temporal bone anomaly.  相似文献   

Effects of temperature on sex determination in marbled sole Limanda yokohamae   总被引：1，自引：0，他引：1  

R IE  GOTO  T AKAAKI  KAYABA  S HINJI  ADACHI  K OHEI  YAMAUCHI 《Fisheries Science》2000,66(2):400-402

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Equine nonneoplastic abnormal ovary in a draft mare with high serum anti-Müllerian hormone: a case study     

Akira GOTO  Masaaki TAGAMI  Fumiki KATO  Tsukasa SUZUKI  Takashi YAMAGA  Harutaka MURASE  Fumio SATO  Munkhtuul TSOGTGEREL  Tadamasa NIIKURA  Tomoe MORIYAMA  Akiko CHIBA  Ken-ichi WATANABE  Nao TSUZUKI  Yasuo NAMBO 《Journal of Equine Science》2021,32(4):147

We performed a standing hand-assisted laparoscopic ovariectomy in a draft mare that presented with high serum anti-Müllerian hormone (AMH) level and had an enlarged single cystic ovary. Histopathological examination revealed no tumor cell proliferation in the ovary, but the presence of a large ovarian cyst was confirmed. In the diagnosis of abnormal ovaries in mares, a comprehensive assessment should be performed, including the monitoring of ovarian morphology and biomarkers over time, to determine the disease prognosis and treatment plan. The case of this mare with a nonneoplastic abnormal ovary and increased serum AMH level was rare. We suggest that standing hand-assisted laparoscopic ovariectomy is useful for the removal of large ovaries in draft mares.  相似文献   

Numerical simulation of the migration and distribution of diamond squid (Thysanoteuthis rhombus) in the southwest Sea of Japan     

G. ONITSUKA  N. HIROSE  K. MIYAHARA  T. OTA  J. HATAYAMA  Y. MITSUNAGA  T. GOTO 《Fisheries Oceanography》2010,19(1):63-75

To elucidate the effects of hydrographic conditions on the migration and distribution of diamond squid ( Thysanoteuthis rhombus ) in the Sea of Japan, two numerical experiments were conducted using a Lagrangian particle-tracking model. First, Lagrangian simulations with different horizontal swimming speeds were conducted in the area off the San-in coast, the southwest Sea of Japan. The comparison results of simulations and experimental tagging records from 2003 suggest that horizontal movement of diamond squid over a few days is influenced by individual swimming, but long-term migration over more than about 10 days is dependent on the ambient current field. Secondly, further Lagrangian simulations with biological processes, forced by different hydrographic conditions in 2003–2005, were conducted. Temporal variations in distributions and sizes calculated by the model corresponded approximately to those of catch data. A large number of particles simulating squid were distributed over the nearshore region from the San-in coast to the Noto Peninsula during September–November every year. Differences in the migration route and distribution of particles among years were closely related to differences in hydrographic conditions such as the position of the main stream of the Tsushima Warm Current. Prediction of the catch off the San-in coast might be improved by considering hydrographic conditions in the southwest Sea of Japan.  相似文献   

Regeneration of protoplasts from hyphal strands ofVolvariella volvacea     

Renato G. Reyes  Fumio Eguchi  Tomoaki Iijima  Miyato Higaki 《Journal of Wood Science》1998,44(5):401-407

A series of experiments on the preparation and regeneration of protoplasts from hyphal strands ofVolvariella volvacea (Bull. ex. Fr.) Singer were conducted with the aim of optimizing the conditions for its efficient regeneration. One commercial (Vvcl) and two wild (EAAC-0001 and EAAC-0002) strains ofV. volvacea from the Philippines were used and subjected to varying conditions to determine the most efficient means for regeneration of their protoplasts. The effects of age and type of strain, pH, type and concentration of osmotic stabilizer, enzymatic composition, treatment time, temperature, reciprocal frequency during enzymatic lysis of the cell wall, and centrifugation conditions were investigated. Results showed that the three strains ofV. volvacea had varying responses in terms of yield, size, and ability of their protoplasts to regenerate into the protoplast regeneration medium. Among the three strains, EAAC-0002 had the highest rate of regeneration. The 5-day-old culture ofV. volvacea, when subjected to a combination of 2% Novozyme 234 and 0.2% chitinase in 0.6M mannitol (pH 6.0) for 3 h at 30°C, 90 strokes/min and centrifuged at 1100 g for 10 min; produced an efficient yield of protoplasts with a relatively high regeneration rate.Part of this paper was presented at the 47th annual meeting of the Japan Wood Research Society, Kochi, Japan, April 2–5, 1997  相似文献   

Sinapyl alcohol-specific peroxidase isoenzyme catalyzes the formation of the dehydrogenative polymer from sinapyl alcohol     

Wataru Aoyama  Shinya Sasaki  Shigeki Matsumura  Thoru Mitsunaga  Hirofumi Hirai  Yuji Tsutsumi  Tomoaki Nishida 《Journal of Wood Science》2002,48(6):497-504

Two peroxidases, CWPO-A and CWPO-C, were isolated from the cell walls of poplar (Populus alba L.) callus culture. The cationic CWPO-C showed a strong preference for sinapyl alcohol over coniferyl alcohol as substrate. Thus, the monolignol utilization of CWPO-C is unique compared with other peroxidases, including anionic CWPO-A and horseradish peroxidase (HRP). CWPO-C polymerized oligomeric sinapyl alcohol (S-oligo) and sinapyl alcohol, producing a polymer of greater molecular weight. In contrast, HRP, which is specific to coniferyl alcohol, produced sinapyl alcohol dimers, rather than catalyzing polymerization. Adding coniferyl alcohol as a radical mediator in the HRP-mediated reaction did not result in S-oligo polymerization. This report shows that CWPO-C is an isoenzyme specific to sinapyl alcohol that polymerizes oligomeric lignols. Its catalytic activity toward oligomeric lignols may be related to the lignification of angiosperm woody plant cell walls.Part of this study was presented at the 45th Lignin Symposium, Matsuyama, October 2000  相似文献   

Treatment of poplar callus with ferulic and sinapic acids II: effects on related monolignol biosynthetic enzyme activities     

Katsuyoshi Hamada  Yuji Tsutsumi  Tomoaki Nishida 《Journal of Wood Science》2003,49(4):366-370

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Regulation of granulosa cell apoptosis by death ligand–receptor signaling     

Fuko MATSUDA-MINEHATA  Akihisa MAEDA  Yuan CHENG  Takafumi SAI  Hiroshi GONDA  Yasufumi GOTO  Noboru MANABE 《Animal Science Journal》2008,79(1):1-10

Several hundred thousand primordial follicles are present in the mammalian ovary, however, only 1% develop to the preovulatory stage and finally ovulate. The remainder will be eliminated via a degenerative process called ‘atresia’. The endocrinological regulatory mechanisms involved in follicular development and atresia have largely been characterized but the precise temporal and molecular mechanisms involved in the regulation of these events remain unknown. Many recent studies suggest that apoptosis in ovarian granulosa cells plays a crucial role in follicular atresia. Notably, death ligand‐receptor interaction and subsequent intracellular signaling have been demonstrated to be the key mechanisms regulating granulosa cell apoptosis. In this review we provide an overview of granulosa cell apoptosis regulated by death ligand‐receptor signaling. The roles of death ligands and receptors [Fas ligand (FasL)]‐Fas, tumor necrosis factor α (TNFα)‐TNF receptor and TNFα‐related apoptosis‐inducing ligand (TRAIL)‐TRAIL receptor (TRAILR)] and intracellular death‐signal mediating molecules (Fas‐associated death domain protein), TNF receptor 1‐associated death domain protein, caspases, apoptotic protease‐activating factor 1, TNFR‐associated factor 2 and cellular FLICE‐like inhibitory protein in granulosa cells are discussed.  相似文献