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111.
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Kusuhara H Hohdatsu T Seta T Nemoto K Motokawa K Gemma T Watanabe R Huang C Arai S Koyama H 《Veterinary microbiology》2007,120(3-4):217-225
Feline immunodeficiency virus (FIV) vaccine, Fel-O-Vax FIV, was released for sale in the US in 2002. The antibodies of vaccinated cats interfere with serological assays by currently available FIV diagnostic kits. In this study, we investigated whether it is possible to distinguish serologically cats vaccinated with Fel-O-Vax FIV from cats experimentally or naturally infected with FIV. A total of 153 sera taken from 97 cats were used as serum samples. Enzyme linked immunosorbent assay (ELISA) was performed using whole FIV antigen and formalin treated whole FIV antigen, recombinant-gag (r-gag) antigen, and transmembrane (TM) peptide. Statistical analysis was performed using ELISA optical density (O.D.) values obtained with each antigen as variables. Except for the ELISA O.D. values obtained with r-gag antigen, a significant difference in ELISA O.D. values was observed between the vaccinated and the infected groups. However, it was not possible to distinguish both groups unequivocally. Using discriminant analysis, it was possible to distinguish the two groups with an accuracy of 97.1% with two discriminating variables (ELISA O.D. values obtained with formalin treated whole FIV antigen, and TM peptide), 97.8% with three discriminating variables (ELISA O.D. values obtained with whole FIV antigen, formalin treated whole FIV antigen, and TM peptide). Therefore, it was considered possible to distinguish cats vaccinated with Fel-O-Vax FIV from FIV-infected cats by ELISA using two types of antigens including formalin treated whole FIV antigen and TM peptide, or three types of antigens including formalin treated whole FIV antigen, TM peptide and whole FIV antigen. 相似文献
113.
Thi Thanh Huong CHU Takako MURANO Yukiko UNO Tatsufumi USUI Tsuyoshi YAMAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(11):1397-1403
Dermanyssus gallinae, the poultry red mite, is an obligatory
blood-sucking ectoparasite. The genetic diversity of D. gallinae has been
examined in some countries, but so far not in Asian countries. Here, we sequenced a part
of the mitochondrial cytochrome oxidase subunit I (COI) and16S rRNA genes and nuclear
internal transcribed spacers (ITS) region in 239 mite samples collected from 40
prefectures throughout Japan. The COI and 16S rRNA nucleotide sequences were classified
into 28 and 26 haplotypes, respectively. In phylogenetic trees, the haplotypes clustered
into 2 haplogroups corresponding to haplogroups A and B, which were previously reported.
Haplogroups A and B were further subdivided into sub-haplogroups AJ1 and AJ2, and BJ1 and
BJ2, respectively. In both trees, the sequences of haplotypes in AJ1 and BJ2 were
relatively distant from those reported in other countries, while some sequences in AJ2 and
BJ1 were identical to those in Europe. In addition, the ITS sequences were classified into
two sequences, and both sequences were closely related to the sequences found in European
countries. These findings indicate a possibility of international oversea transmission of
D. gallinae. 相似文献
114.
115.
Fumiko?KimuraEmail author Yasushi?Endo Kenshiro?Fujimoto Nobushige?Doisaki Tsuyoshi?Koriyama 《Fisheries Science》2005,71(2):431-440
Long-chain polyunsaturated fatty acids (LCPUFA) with 20 or 22 carbons are considered important to the development of infants
and sometimes added to infant formulae. In this study, two characteristic sources of n-3 LCPUFA (fish oil and microalgal oil)
were orally administrated to rat pups of mildly n-3 PUFA — deficient dams to compare the consequences of the administration.
The milk from the dams fed a n-3 PUFA — restricted diet contained less n-3 LCPUFA than that of the dams fed a control diet.
Pups were administered 1 mg/g weight of the test oil at the age of 5–7 days. At the age of 7 days, they were sacrificed before
or after the administration and fatty acid compositions of the stomach and serum lipid were studied. The administration changed
docosahexaenoic acid (DHA; 22:6n−3) levels in the stomach contents and serum lipids with time. Eicosapentaenoic acid (EPA;
20:5n−3) levels increased immediately after the administration of fish oil. The administration of microalgal oil also affected
the serum lipid EPA level, in spite of a lack of EPA. In this study, both oils effectively supplemented DHA. Fish oil returned
the serum EPA level close to the control value while microalgae oil had little effect. 相似文献
116.
Microalgae tend to accumulate lipids as an energy storage material in the specific organelle, oleosomes. Current studies have demonstrated that lipids derived from microalgal oleosomes are a promising source of biofuels, while the oleosome formation mechanism has not been fully elucidated. Oleosome-associated proteins have been identified from several microalgae to elucidate the fundamental mechanisms of oleosome formation, although understanding their functions is still in infancy. Recently, we discovered a diatom-oleosome-associated-protein 1 (DOAP1) from the oleaginous diatom, Fistulifera solaris JPCC DA0580. The DOAP1 sequence implied that this protein might be transported into the endoplasmic reticulum (ER) due to the signal sequence. To ensure this, we fused the signal sequence to green fluorescence protein. The fusion protein distributed around the chloroplast as like a meshwork membrane structure, indicating the ER localization. This result suggests that DOAP1 could firstly localize at the ER, then move to the oleosomes. This study also demonstrated that the DOAP1 signal sequence allowed recombinant proteins to be specifically expressed in the ER of the oleaginous diatom. It would be a useful technique for engineering the lipid synthesis pathways existing in the ER, and finally controlling the biofuel quality. 相似文献
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118.
Shibata Saho Ono Tsuyoshi Kobayashi Yoshiko Miyagawa Itsuha Mukai Akitsugu Ichioka Youzou Takenaka Izumi Nakano Hideto Hirooka Yuuri 《Journal of General Plant Pathology》2021,87(5):322-325
Journal of General Plant Pathology - In June 2017, deer-horn-like galls covered with a whitish, powdery fungal layer were found on the trunks of Cinnamomum pseudopedunculatum (Lauraceae) in... 相似文献
119.
Yoshiyuki Yanase Masahiro Miura Yoshihisa Fujii Shogo Okumura Tsuyoshi Yoshimura 《Journal of Wood Science》2013,59(3):243-248
A gas detection apparatus equipped with a semiconductor gas sensor was employed for qualitative and quantitative measurement of hydrogen and methane emitted by termites. A gas sample of 2.5 ml was injected into the semiconductor gas sensor through the gas detection apparatus, and the maximum voltage of the sensor was converted into gas concentration. The gas samples were collected from three distinct experiments: (1) five combinations of workers and soldiers of Coptotermes formosanus with and without a wood specimen; (2) C. formosanus under six temperature conditions; and (3) four different termite species, C. formosanus, Reticulitermes speratus, Incisitermes minor, and Zootermopsis nevadensis. The hydrogen and methane concentrations increased with an increase in the number of termites. Concentrations were higher in samples with a wood specimen than without it. Both hydrogen and methane concentrations were the highest for the samples at 35 °C and were lowest at 15 and 5 °C. The concentrations were very low at 45 °C because all the termites had died in a few hours. The concentrations of hydrogen and methane were highest for Z. nevadensis, the dampwood termite, among the four species, and no methane was detected for I. minor, the drywood termite, at 28 °C and 75 % RH. 相似文献
120.