Genotyping of Bacillus anthracis Isolated from Croatia and Bosnia and Herzegovina     

I. Steiner  I. Račić  S. Špičić  B. Habrun 《Zoonoses and public health》2013,60(3):202-208

Anthrax is a serious disease caused by Bacillus anthracis. Humans can become infected by handling products from infected animals, by breathing spores and rarely by eating undercooked meat from infected animals. The genome of B. anthracis is highly monomorphic and thus shows very low DNA sequence variation. We analysed the molecular characteristics of 12 B. anthracis isolates from outbreaks in Croatia and Bosnia and Herzegovina, which have occurred during the past 10 years along with two vaccine strains. Genotyping system based on variable‐number tandem repeat analysis at six loci revealed that six isolates belong to genotype from the A1.a cluster whilst six isolates relate to the B2 cluster, compared to 89 previously described genotypes. The distribution of two evolutionarily distant clusters suggests an introduction of B. anthracis to this area in at least two separate events.  相似文献   

Nitrogen retention and plant uptake on a highly weathered central Amazonian Ferralsol amended with compost and charcoal     

Christoph Steiner  Bruno Glaser  Wenceslau Geraldes Teixeira  Johannes Lehmann  Winfried E.H. Blum  Wolfgang Zech 《植物养料与土壤学杂志》2008,171(6):893-899

Leaching losses of N are a major limitation of crop production on permeable soils and under heavy rainfalls as in the humid tropics. We established a field trial in the central Amazon (near Manaus, Brazil) in order to study the influence of charcoal and compost on the retention of N. Fifteen months after organic‐matter admixing (0–0.1 m soil depth), we added ¹⁵N‐labeled (NH₄)₂SO₄ (27.5 kg N ha^–1 at 10 atom% excess). The tracer was measured in top soil (0–0.1 m) and plant samples taken at two successive sorghum (Sorghum bicolor L. Moench) harvests. The N recovery in biomass was significantly higher when the soil contained compost (14.7% of applied N) in comparison to only mineral‐fertilized plots (5.7%) due to significantly higher crop production during the first growth period. After the second harvest, the retention in soil was significantly higher in the charcoal‐amended plots (15.6%) in comparison to only mineral‐fertilized plots (9.7%) due to higher retention in soil. The total N recovery in soil, crop residues, and grains was significantly (p < 0.05) higher on compost (16.5%), charcoal (18.1%), and charcoal‐plus‐compost treatments (17.4%) in comparison to only mineral‐fertilized plots (10.9%). Organic amendments increased the retention of applied fertilizer N. One process in this retention was found to be the recycling of N taken up by the crop. The relevance of immobilization, reduced N leaching, and gaseous losses as well as other potential processes for increasing N retention should be unraveled in future studies.  相似文献   

Effects of brine salting with regard to raw material variation of Atlantic salmon (Salmo salar) muscle investigated by Fourier transform infrared microspectroscopy     

Böcker U  Kohler A  Aursand IG  Ofstad R 《Journal of agricultural and food chemistry》2008,56(13):5129-5137

Atlantic salmon fillets differing with regard to raw material characteristics (prerigor, postrigor, frozen/thawed) and salt content were investigated by FT-IR microspectroscopy and light microscopy. Local variation within each salmon fillet was further taken into account by sampling from the head and tail part separately as they vary in fat and moisture content. The highest salt uptake was achieved for frozen/thawed quality during brine-salting with 16% NaCl for 4 h, while the uptake was least for prerigor fish. At the same time, salting caused muscle fiber swelling of about 10% for both frozen/thawed and postrigor qualities. Differences in the FT-IR amide I spectral region were observed implying a change in the muscle protein secondary structure. Prerigor was least affected by brine-salting, having a final salt concentration of 2.2%, while postrigor had a NaCl content of 3.0% and frozen/thawed of 4.1%. Local variation within the fillets had an effect on the amide I absorption characteristics before as well as after salting. Salt uptake of the samples was affected by raw material quality and at the same time the degree of swelling of the myofibers was influenced by raw material character.  相似文献   

Charcoal and smoke extract stimulate the soil microbial community in a highly weathered xanthic Ferralsol   总被引：2，自引：0，他引：2  

Christoph Steiner  Keshav C. Das  Marcos Garcia  Bernhard Frster  Wolfgang Zech 《Pedobiologia》2008,51(5-6):359-366

The influence of charcoal and smoke condensates (pyroligneous acid, PA) on microbial activity in a highly weathered Amazonian upland soil was assessed via measurements of basal respiration (BR), substrate-induced respiration (SIR), and exponential population increase after substrate addition. PA extracts are commonly used for fertilizer or as pest control in Brazil, where phosphorus (P) availability and nitrogen (N) leaching are among the most severe limitations for agriculture. Microbes play an important role in nutrient cycling and solubilizing of phosphate. BR, microbial biomass, population growth and the microbe's efficiency (expressed by the metabolic quotient) increased linearly and significantly with increasing charcoal concentrations (50, 100 and 150 g kg⁻¹ soil). Application of PA caused a sharp increase in all parameters. We suppose that the condensates from smoke contain easily degradable substances and only small amounts of inhibitory agents, which could be utilized by the microbes for their metabolism.  相似文献   

Permanence of soil surface crusts on abandoned farmland in the Central Ebro Basin/Spain     

Johannes B. Ries  Ulrike Hirt 《CATENA》2008

Surface crusts are frequently found on fallow land in the semi-arid Ebro Basin (Spain) and are an important factor in land degradation. Soil surface sealing leads to a decrease in infiltration rates and a consequent increase in runoff, thereby accelerating sheet wash and rill erosion. Thin sections were used to analyse the development and structures of the different crusts found across the ridge/furrow field pattern. Rainfall simulations experiments and infiltration measurements show the runoff generation and the soil erosion rates on the crusts. The spatial distribution of crusts was documented using large-scale aerial photographs, taken from a remote-controlled hot air blimp.  相似文献   

Prevalence and identification of fungal DNA in the small intestine of healthy dogs and dogs with chronic enteropathies     

Suchodolski JS  Morris EK  Allenspach K  Jergens AE  Harmoinen JA  Westermarck E  Steiner JM 《Veterinary microbiology》2008,132(3-4):379-388

Limited information is available about the prevalence and phylogenetic classification of fungal organisms in the gastrointestinal tract of dogs. Also, the impact of fungal organisms on gastrointestinal health and disease is not well understood. The aim of this study was to evaluate the prevalence of fungal DNA in the small intestine of healthy dogs and dogs with chronic enteropathies. Small intestinal content was analyzed from 64 healthy and 71 diseased dogs from five different geographic locations in Europe and the USA. Fungal DNA was amplified with panfungal primers targeting the internal transcriber spacer (ITS) region. PCR amplicons were subjected to phylogenetic analysis. Fungal DNA was detected in 60.9% of healthy dogs and in 76.1% of dogs with chronic enteropathies. This prevalence was not significantly different between the two groups (p=0.065). Fungal DNA was significantly more prevalent in mucosal brush samples (82.8%) than in luminal samples (42.9%; p=0.002). Sequencing results revealed a total of 51 different phylotypes. All sequences belonged to two phyla and were classified as either Ascomycota (32 phylotypes) or Basidiomycota (19 phylotypes). Three major classes were identified: Saccharomycetes, Dothideomycetes, and Hymenomycetes. The most commonly observed sequences were classified as Pichia spp., Cryptococcus spp., Candida spp., and Trichosporon spp. Species believed to be clinically more important were more commonly observed in diseased dogs. These results indicate a high prevalence and diversity of fungal DNA in the small intestine of both healthy dogs and dogs with chronic enteropathies. The canine gastrointestinal tract of diseased dogs may harbor opportunistic fungal pathogens.  相似文献   

Humoral immune response to avian influenza vaccination over a six-month period in different species of captive wild birds     

Furger M  Hoop R  Steinmetz H  Eulenberger U  Hatt JM 《Avian diseases》2008,52(2):222-228

In December 2005, the four major Swiss zoos carried out the vaccination of selected zoo birds with the adjuvant inactivated vaccine H5N2 Nobilis influenza. Pre- and post-vaccination antibody titers were determined either by hemagglutination inhibition (HI) test (non-Galliformes) or by enzyme linked immunosorbent assay (ELISA) (Galliformes) at Week 0, 5, 10, and 26 (Day 0-1, 35-36, 70-71, and 182 respectively) to determine the humoral immune response to H5 antigen. After the first vaccination, the overall geometric mean titer of non-Galliformes was 65 (n = 142), which increased to 187 (n = 139) after booster vaccination and dropped to 74 (n = 65) six months after first vaccination. For the Galliformes group, the mean titers were found to be 2.09 at Week 5 (n = 119), 3.24 at Week 10 (n = 113), and 1.20 at Week 26 (n = 39). Within the non-Galliformes, significant differences in geometric mean titers were found among different species representatives. In general, the flamingos (Phoenicopteriformes) showed a strong response to vaccination, reaching a geometric mean titer of 659 at Week 10, while the Sphenisciformes did not show high antibody titers even after booster vaccination, reaching a maximum geometric mean titer of only 65. Based on the antibody titer profiles of all investigated species, we recommend at least annual revaccination for the species that we investigated.  相似文献   

Results of histopathology,immunohistochemistry, and molecular clonality testing of small intestinal biopsy specimens from clinically healthy client‐owned cats     

Sina Marsilio  Mark R. Ackermann  Jonathan A. Lidbury  Jan S. Suchodolski  Jrg M. Steiner 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(2):551-558

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Untargeted metabolomic profiling of serum from dogs with chronic hepatic disease     

Yuri A. Lawrence  Micah A. Bishop  Julia B. Honneffer  Audrey K. Cook  Aline Rodrigues‐Hoffmann  Jrg M. Steiner  Jan S. Suchodolski  Jonathan A. Lidbury 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(3):1344-1352

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Partial analytical validation of the VetScan cPL rapid test     

Joerg M. Steiner  Phillip Guadiano  Robynne R. Gomez  Jan S. Suchodolski  Jonathan A. Lidbury 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2019,48(4):683-690

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