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131.
132.
Biochemical marker endopeptidase EP-1 was used to determine that an eyespot resistance gene, transferred from Aegilops ventricosa to the hexaploid wheat line ‘H-93-70’ is located on the long arm of the chromosome 7D. 相似文献
133.
Usama Ramadan Abdelmohsen Cheng Cheng Christina Viegelmann Tong Zhang Tanja Grkovic Safwat Ahmed Ronald J. Quinn Ute Hentschel RuAngelie Edrada-Ebel 《Marine drugs》2014,12(3):1220-1244
High resolution Fourier transform mass spectrometry (HRFTMS) and nuclear magnetic resonance (NMR) spectroscopy were employed as complementary metabolomic tools to dereplicate the chemical profile of the new and antitrypanosomally active sponge-associated bacterium Actinokineospora sp. EG49 extract. Principal Component (PCA), hierarchical clustering (HCA), and orthogonal partial least square-discriminant analysis (OPLS-DA) were used to evaluate the HRFTMS and NMR data of crude extracts from four different fermentation approaches. Statistical analysis identified the best culture one-strain-many-compounds (OSMAC) condition and extraction procedure, which was used for the isolation of novel bioactive metabolites. As a result, two new O-glycosylated angucyclines, named actinosporins A (1) and B (2), were isolated from the broth culture of Actinokineospora sp. strain EG49, which was cultivated from the Red Sea sponge Spheciospongia vagabunda. The structures of actinosporins A and B were determined by 1D- and 2D-NMR techniques, as well as high resolution tandem mass spectrometry. Testing for antiparasitic properties showed that actinosporin A exhibited activity against Trypanosoma brucei brucei with an IC50 value of 15 µM; however no activity was detected against Leishmania major and Plasmodium falciparum, therefore suggesting its selectivity against the parasite Trypanosoma brucei brucei; the causative agent of sleeping sickness. 相似文献
134.
Bournaud F Duc PA Brinks E Boquien M Amram P Lisenfeld U Koribalski BS Walter F Charmandaris V 《Science (New York, N.Y.)》2007,316(5828):1166-1169
Recycled dwarf galaxies can form in the collisional debris of massive galaxies. Theoretical models predict that, contrary to classical galaxies, these recycled galaxies should be free of nonbaryonic dark matter. By analyzing the observed gas kinematics of such recycled galaxies with the help of a numerical model, we demonstrate that they do contain a massive dark component amounting to about twice the visible matter. Staying within the standard cosmological framework, this result most likely indicates the presence of large amounts of unseen, presumably cold, molecular gas. This additional mass should be present in the disks of their progenitor spiral galaxies, accounting for a substantial part of the so-called missing baryons. 相似文献