Silk genes support the single origin of orb webs     

Garb JE  Dimauro T  Vo V  Hayashi CY 《Science (New York, N.Y.)》2006,312(5781):1762

The orb web is a spectacular evolutionary innovation that enables spiders to catch flying prey. This elegant, geometric structure is woven with silk fibers that are renowned for their superior mechanical properties. We used silk gland expression libraries to address a long-standing controversy concerning the evolution of the orb-web architecture. Contrary to the view that the orb-web design evolved multiple times, we found that the distribution and phylogeny of silk proteins support a single, ancient origin of the orb web at least 136 million years ago. Furthermore, we substantially expanded the repository of silk sequences that can be used for the synthesis of high-performance biomaterials.  相似文献   

Inheritance of rol-genes from Agrobacterium rhizogenes through two generations in Kalancho?     

Henrik Lütken  Sabá Victoria Wallstr?m  Erik Bj?rn Jensen  Brian Christensen  Renate Müller 《Euphytica》2012,188(3):397-407

Transformation of Kalancho? blossfeldiana ‘Molly’ using the naturally occurring bacterium Agrobacterium rhizogenes is a non-GMO strategy to breed compact plants. In the present study, crosses resembling a commercial breeding strategy were made to determine if the improved ornamental quality observed in the T1 generation was inherited to the offspring. F1 lines were produced by crossing the commercial Kalancho? cultivar ‘Sarah’ with a selected T1 line. Subsequently, F2 populations were produced by self-pollination of individual selected lines. The rol-genes were inherited to the progeny and the presence of rol-genes was confirmed in all F1 and many F2 plant lines exhibiting dwarfism. Screening of F1 and F2 plants showed that the rol-genes were inherited together. Besides decreased plant height, several F1 and F2 lines containing rol-genes exhibited changes in plant diameter, number of branches, flower diameter and time to first open flower and duration of flowering compared to the WT ‘Molly’. Furthermore, increased ethylene tolerance was observed in several lines containing rol-genes compared to the WT ‘Sarah’. Screening of three selected F2 populations derived from self-pollination of F1 lines containing rol-genes indicated a shift in distribution towards a lower mean plant height within the entire plant population compared to a control population of plants without rol-genes. Moreover, one of these F2 populations also exhibited earlier flowering compared to the control population. Compact potted plants and lines without delayed flowering and with improved ethylene tolerance were obtained and are valuable in commercial breeding programmes without using recombinant DNA technology.  相似文献   

Use of aerial thermal imaging to estimate water status of palm trees   总被引：1，自引：0，他引：1  

Y. Cohen  V. Alchanatis  A. Prigojin  A. Levi  V. Soroker  Y. Cohen 《Precision Agriculture》2012,13(1):123-140

A methodology to estimate water status of palm trees from aerial thermal images was developed. Deficit irrigation of 80% in three drip-irrigated date-palm plots in the northern Dead Sea region was manipulated during the winter of 2007 and 2008. An uncooled thermal camera was used for extensive aerial imaging to detect palm trees and pure-canopy pixels by using only aerial thermal images. An automatic procedure, based on watershed segmentation analysis, was developed which enabled detection of all palm trees in the thermal images. Two new methods were developed to select palm trees and pure pixels within them: basin-based and pixel-based. From the temperatures of pure-canopy pixels, significant differences were found between palm trees under commercial and deficit irrigation regimes, in all three plots. Automated detection of canopy, based on aerial thermal images, is a key step towards commercial mapping of within-plot water-status variability. A protocol, based on the developed methodology, was suggested for mapping water status variability in a palm plot, and for irrigation scheduling.  相似文献   

Serum protein concentrations as predictors of serum immunoglobulin G concentration in neonatal foals     

Adriel L. Matthews BS  Victoria H. L. Scott BVetMed  Jarred M. Williams DVM  MS  DACVS  Catherine W. Kohn VMD  DACVIM  Ramiro E. Toribio DVM  MS  PhD  DACVIM 《Journal of Veterinary Emergency and Critical Care》2012,22(5):573-579

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Direct intra‐abdominal pressures and abdominal perfusion pressures in unsedated normal horses     

Victoria H. L. Scott BVetMed 《Journal of Veterinary Emergency and Critical Care》2012,22(4):441-446

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Soil enzymatic activities and microbial biomass in an integrated agroforestry chronosequence compared to monoculture and a native forest of Colombia   总被引：1，自引：0，他引：1  

Victoria E. Vallejo  Fabio Roldan  Richard P. Dick 《Biology and Fertility of Soils》2010,46(6):577-587

Large tracts of land in South American native forests have been converted to monoculture for livestock production which could negatively affect soils and environmental quality. A proposed management alternative is to use agroforestry systems, but little information is available on how they affect the soils. The objective was to assess the effect of a silvopastoral chronosequence in a tropical region of Colombia on soil microbiological and physico/chemical properties. The systems (three replications) were: monoculture grass conventional pasture (CP), native forest (F), and a silvopastoral system (SS) chronosequence with ages of 3 to 6 (SS₃), 8 to 10 (SS₈), or 12 to 15 (SS₁₂) years. Soil responses to these land management were determined by measuring soil chemical (total C and pH), physical (penetrometer resistance and bulk density), and microbiological properties (activities of ß-glucosidase, urease, and alkaline and acid phosphatase and microbial biomass). Because of differences in soil texture across management treatments, microbiological properties were normalized on organic C content basis. SS₁₂ showed the highest microbial biomass and enzyme activities on a per unit C basis and was consistently and significantly different from CP. Additionally, microbiological to C ratios were significantly affected by SS establishment age (P?相似文献   

Comparative performance of tests using cytosolic or outer membrane antigens of Brucella for the serodiagnosis of canine brucellosis     

Wanke MM  Delpino MV  Baldi PC 《Veterinary microbiology》2002,88(4):367-375

Although some ELISA tests using cytoplasmic or outer membrane antigens of Brucella have been developed to improve the diagnosis of canine brucellosis, the performance of these assays has not been compared. In the present study three ELISA tests using lipopolysaccharide (LPS)-free cytoplasmic proteins (CPs) of Brucella abortus, the lumazine synthase (LS) of Brucella spp. or a hot-saline (HS) extract of Brucella canis containing outer membrane antigens were used to test sera from dogs with suspected or confirmed brucellosis (n=36) and from dogs with pathological conditions other than brucellosis (n=212). In the first group the proportion of positive results was 92, 92 and 81% for the ELISAs with HS, CP and LS, respectively, and 94% of the samples were positive by at least one ELISA test. Three dogs that were negative by agglutination (2ME-RSAT) had a positive result by at least one ELISA, and this discrepancy was attributed to the lower analytical sensitivity of agglutination tests. This hypothesis was confirmed by a serological follow-up of seven dogs recently infected with B. canis in three of which the illness was diagnosed earlier by one or more ELISA tests than by 2ME-RSAT. Among dogs having pathological conditions other than brucellosis, specificities were 94.3, 96.7 and 96.7% for the ELISAs with HS, CP and LS, respectively. This study shows that HS-ELISA and CP-ELISA are highly specific and sensitive for the diagnosis of canine brucellosis and can detect the infection by B. canis shortly after the exposure to the pathogen.  相似文献   

Measures of self-efficacy and norms for low-fat milk consumption are reliable and related to beverage consumption among 5th graders at school lunch     

Thompson VJ  Bachman C  Watson K  Baranowski T  Cullen KW 《Public health nutrition》2008,11(4):421-426

OBJECTIVE: To determine the reliability and validity of scales measuring low-fat milk consumption self-efficacy and norms during school lunch among a cohort of 5th graders. DESIGN: Two hundred and seventy-five students completed lunch food records and a psychosocial questionnaire measuring self-efficacy and norms for school lunch low-fat milk consumption during the fall and spring semesters of the 1998-1999 academic year. Test-retest reliability was assessed in participants who also completed the questionnaire in the spring semester (n = 262). Principal component analyses identified and confirmatory factor analyses confirmed latent variables. Bivariate correlations measured construct validity. SETTING: Houston-area middle school. SUBJECTS: Fifth graders (n = 275) from one middle school in southeast Texas. RESULTS: Two scales measuring psychosocial influences of low-fat milk consumption were identified and proved reliable in this population: milk self-efficacy and milk norms. Milk self-efficacy and norms were positively correlated with milk consumption and negatively correlated with consumption of sweetened beverages. CONCLUSIONS: These questionnaires can be used in similar interventions to measure the impact of self-efficacy and norms for drinking low-fat milk during school lunch.  相似文献   

Suitability of enzyme activities for the monitoring of soil quality improvement in organic agricultural systems   总被引：2，自引：1，他引：1  

Roberto García-Ruiz  Victoria Ochoa  M. Beln Hinojosa  Jose Antonio Carreira 《Soil biology & biochemistry》2008,40(9):2137-2145

There is growing interest in the applications of soil enzymes as early indicators of soil quality change under contrasting agricultural management practices. However, despite there being an abundant literature on this subject, most comparative assessments have been based on a limited number of experimental farms and, therefore, conclusions are not as robust as desired. In this study, we compare 18 pairs of organic and neighbouring conventional olive orchards in southern Spain. These sites were selected to allow the definition of the relative contributions of site-landscape features, soil type, and time since organic accreditation and tillage intensity, on the soil quality. Soils were analysed for physico-chemical properties, the activities of dehydrogenase, β-glucosidase, arylsulphatase, acid and alkaline phosphatase, and potential nitrification. The geometric mean of the assayed enzymes (GMea) was validated with an independently performed Principal Component Analysis (PCA), and used as a combined soil quality index. The effects of tillage intensity and time since organic accreditation on the improvement of soil quality were also evaluated within the subset of organic farms. Overall for the 18 sites, contrasted management practices did not differ in their impact on basic soil physico-chemical properties, except for loss of on ignition and available inorganic N which were higher and lower in organic farms, respectively. Organic management resulted in significantly higher soil enzyme activities. However, differences were not significant in some of the paired comparisons when considered individually. This highlights the need for extensive comparative assessment, as in this study, to draw clear conclusions concerning the changes to soil quality under sustainable management practices. The GMea was significantly correlated with the first axis of the PCA and shown to be appropriate for condensing the set of soil enzyme values to a sole numerical value. Soil quality changes in organic versus conventional farms, as measured by the GMea, ranged from −23% to 97%, and was highly dependent on time since organic accreditation (r = 0.88; P < 0.01). On the other hand, tillage intensity clearly tended to delay any progress in soil quality in the organic farms.  相似文献   

Evidence of the role of European wild boar as a reservoir of Mycobacterium tuberculosis complex     

Naranjo V  Gortazar C  Vicente J  de la Fuente J 《Veterinary microbiology》2008,127(1-2):1-9

Bovine tuberculosis (bTB) is caused by Mycobacterium bovis and closely related mycobacteria of the Mycobacterium tuberculosis complex. They have an extensive host range and may cause zoonotic TB. A major obstacle to bTB eradication in livestock is the implication of wildlife in the natural cycle of the pathogen. The identification of wildlife reservoir hosts is crucial for the implementation of effective control measures. The European wild boar (Sus scrofa) is frequently considered a spillover or dead end host rather than a true reservoir, and scientific evidence is conflicting outside Mediterranean Spain. The aim of this review is to update current scientific evidence of the wild boar as a TB reservoir and to underline those aspects that need further research. Evidences supporting that wild boar is a TB reservoir host include: (i) presence of common M. tuberculosis complex genotypes in wild boar, domestic and wild animals and humans, (ii) high prevalence of M. bovis among wild boar in estates fenced for decades in complete absence of contact with domestic livestock, and other wild ungulates (iii) TB lesions are frequently seen in thoracic lymph nodes and lungs, suggesting that respiratory infection and excretion may occur, and (iv) extensive tuberculous lesions in more than one anatomical region occur in a high proportion of juvenile wild boar that probably represents the main source of mycobacterial excretion. Hence, epidemiological, pathological and microbiological evidence strongly suggests that, at least in Spanish Mediterranean ecosystems, wild boar are able to maintain TB infection in the wild and are most probably able to transmit the disease to other species, acting as a true wildlife reservoir. These results expand the list of wildlife species that act as natural reservoirs of TB in different parts of the world and suggest the need to control the infection in wild boar populations for the complete eradication of the disease in Spain.  相似文献