Use of Agrobacterium tumefaciens-mediated transformation for the inactivation of pectinase genes in Colletotrichum lindemuthianum     

da Silva  Leandro Lopes  Rosa  Rafael Oliveira  Garcia  Ediones Amaro  Teixeira  Janaina Aparecida  Mendes  Tiago Antônio de Oliveira  de Queiroz  Marisa Vieira 《European journal of plant pathology / European Foundation for Plant Pathology》2022,162(3):595-608

European Journal of Plant Pathology - The fungus Colletotrichum lindemuthianum has genes that encode different enzymes involved in plant cell wall degradation such as pectinases, which are...  相似文献   

Structural biology. Brazil network sees the light     

Vieira CL 《Science (New York, N.Y.)》2001,293(5535):1578

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Brazil. Tough placebo rules leave scientists out in the cold     

Vieira CL 《Science (New York, N.Y.)》2002,295(5553):264

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Detection of a novel hemoplasma based on 16S rRNA gene DNA in captive and free-ranging capybaras (Hydrochaeris hydrochaeris)     

Rafael F.C. Vieira  Marcelo B. Molento  Leonilda C. dos Santos  Wanderlei Moraes  Zalmir S. Cubas  Andrea P. Santos  Ana M.S. Guimaraes  Ahmed Mohamed  Ivan R. Barros Filho  Alexander W. Biondo  Joanne B. Messick 《Veterinary microbiology》2009,139(3-4):410-413

Two different species of hemoplasmas, Mycoplasma coccoides and M. haemomuris, are known to infect small rodents such as mice and rats. However, there are no previous reports of hemoplasma infection in capybara (Hydrochaeris hydrochaeris). The aim of our study was to determine whether these hemoplasmas might infect capybaras from Southern Brazil. Blood samples from 31 animals: 10 captive and 21 free-ranging capybaras were collected and packed cell volume and total plasma protein were measured. DNA was extracted and PCR assays for M. coccoides and M. haemomuris were performed. Using the M. coccoides-PCR assay 64% of the capybaras were positive, 80% free-ranging and 30% from captive animals. The prevalence of infection between the groups was significantly different (p = 0.001). Sequencing of the nearly entire 16S rRNA gene from the positive samples suggested a novel hemoplasma isolate with identity of 92% with M. coccoides and 86% with M. haemomuris. All capybara samples were negative for M. haemomuris infection. DNA of a housekeeping gene was successfully amplified from all samples. This is the first evidence of a hemoplasma infection in capybaras.  相似文献   

Anthelmintic activity of Cocos nucifera L. against sheep gastrointestinal nematodes     

L.M.B. Oliveira  C.M.L. Bevilaqua  C.T.C. Costa  I.T.F. Macedo  R.S. Barros  A.C.M. Rodrigues  A.L.F. Camurça-Vasconcelos  S.M. Morais  Y.C. Lima  L.S. Vieira  A.M.C. Navarro 《Veterinary parasitology》2009

The development of anthelmintic resistance has made the search for alternatives to control gastrointestinal nematodes of small ruminants imperative. Among these alternatives are several medicinal plants traditionally used as anthelmintics. This work evaluated the efficacy of Cocos nucifera fruit on sheep gastrointestinal parasites. The ethyl acetate extract obtained from the liquid of green coconut husk fiber (LGCHF) was submitted to in vitro and in vivo tests. The in vitro assay was based on egg hatching (EHT) and larval development tests (LDT) with Haemonchus contortus. The concentrations tested in the EHT were 0.31, 0.62, 1.25, 2.5 and 5 mg ml⁻¹, while in the LDT they were 5, 10, 20, 40 and 80 mg ml⁻¹. The in vivo assay was a controlled test. In this experiment, 18 sheep infected with gastrointestinal nematodes were divided into three groups (n = 6), with the following doses administered: G1—400 mg kg⁻¹ LGCHF ethyl acetate extract, G2—0.2 mg kg⁻¹ moxidectin (Cydectin^®) and G3—3% DMSO. The worm burden was analyzed. The results of the in vitro and in vivo tests were submitted to ANOVA and analyzed by the Tukey and Kruskal–Wallis tests, respectively. The extract efficacy in the EHT and LDT, at the highest concentrations tested, was 100% on egg hatching and 99.77% on larval development. The parameters evaluated in the controlled test were not statistically different, showing that despite the significant results of the in vitro tests, the LGCHF ethyl acetate extract showed no activity against sheep gastrointestinal nematodes.  相似文献   

Reproductive Performance of Gilts with Similar Age but with Different Growth Rate at the Onset of Puberty Stimulation   总被引：1，自引：0，他引：1  

R Kummer  ML Bernardi  AC Schenkel  WS Amaral Filha  I Wentz  FP Bortolozzo 《Reproduction in domestic animals》2009,44(2):255-259

The aim of this study was to evaluate the reproductive performance of gilts that had a similar age but different weights at the onset of puberty stimulation by boar exposure at 144 days. Gilts were divided into two groups according to their lifetime growth rate from birth to approximately 144 days of age. Mean growth rates at this moment were 577 and 724 g/day for group 1 (G1; n = 58) and group 2 (G2; n = 58), respectively. After selection, gilts were weighed at approximately 155, 165 and 175 days of age, on the insemination day and at slaughter. Gilts were inseminated, on average, at 193 days of age and were slaughtered 32 days after insemination, when the number of corpora lutea and embryos were recorded. Higher growth rate gilts (G2) reached puberty earlier (155.3 vs 164.1 days; p < 0.01). More gilts of G2 group attained puberty by 190 days of age (p = 0.004) than G1 gilts (95%; 55/58 vs 76%; 44/58). The anoestrous rate, until 60 days after the onset of boar exposure was higher (p < 0.01) in G1 (19.0%; 11/58) than in G2 (3.4%; 2/58) group. However, there were no differences in the pregnancy rate (90.7 vs 94.5), ovulation rate (15.9 vs 16.5), total embryos (12.9 vs 11.7), viable embryos (12.0 vs 11.1) and embryo survival (73.7% vs 68.5%), between G1 gilts and G2 gilts, respectively (p > 0.05). High growth rate gilts attain puberty earlier and have a lower anoestrous rate than low growth rate gilts.  相似文献