14-3-3 protein down-regulates key enzyme activities of nitrate and carbohydrate metabolism in potato plants   总被引：1，自引：0，他引：1  

Zuk M  Weber R  Szopa J 《Journal of agricultural and food chemistry》2005,53(9):3454-3460

The 14-3-3 protein is one of the best candidates for coordinating all plant metabolic pathways. To verify this suggestion transgenic potato plants with repression of one (J4 and J5 plants), two (G1 plants), and six (G3 plants) constitutive 14-3-3 protein isoforms as well as plants overexpressing the 14-3-3 protein were studied. Reduction in the 14-3-3 protein level in the J4 and J5 transformants, the G1 transformants, and the G3 transformants was close to 29, 41.5, 38, and 55%, respectively. In the case of the 14-3-3 overexpressing plants (J2), a 30% increase in protein content was detected. Changes in nitrate reductase (NR), sucrose phosphate synthase (SPS), and starch synthase (SS) activities in the transgenic plants perfectly reflect the overall 14-3-3 protein level. The highest increase in enzyme activities was observed for the G3 plants and the lowest for the J4 transformants. The same was detected for the measured metabolites. The highest increase in the protein, starch, and sucrose levels was detected in the tubers from the G3 transgenic plants. Because there was almost no change in the isoform ratio in the transgenic plants when compared to the control, it is suggested that it is the overall content of the 14-3-3 protein, rather than the content of particular isoforms, which plays a crucial role in the regulation of enzyme activities and thus in metabolite synthesis. The properties of the 14-3-3 overexpressing plants are very similar to those of the control ones, suggesting that the protein is in excess in the nontransformants and a further increase in its content is not recognized by cell metabolism. A considerable influence of the 14-3-3 protein level on potato plant metabolism was demonstrated. This effect was observed in key metabolic enzyme activities and metabolite content as well. A high variability between mean values, representing individual transgenes, with respect to nitrate reductase, sucrose phosphate synthase, and starch synthase activities in the examined genotypes was noted. These changes were closely correlated with metabolite levels, among them protein, starch, reducing sugars, and sucrose. The results obtained for the five types of transgenic potato plants in comparison with the control were statistically assessed using discriminate function and cluster analyses.  相似文献   

Noack,S.: Statistische Auswertung von Me& und Versuchsdaten mit Taschenrechner und Tischcomputer. 582 S., 58 Abb., 35 Tab. für Programme, 77 Tab., Leineneinband,DM 49,-, Verlag de Gruyter,Berlin 1980.     

W. E. Weber 《植物养料与土壤学杂志》1980,143(6):743-743

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The detection of Mycobacterium bovis reinfection in a cattle herd     

A Weber  H Lutz  K Bauer 《Berliner und Münchener tier?rztliche Wochenschrift》1988,101(9):302-307

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The rapid identification of Escherichia coli using Bactident E. coli     

A Weber  R Sch?fer 《Berliner und Münchener tier?rztliche Wochenschrift》1989,102(1):4-5

165 (93.7%) of 175 E. coli strains isolated from various materials of different animal species were correctly identified as E. coli with help of commercially available Bactident E. coli (E. Merck, D-6100 Darmstadt). Further 52 strains of the family of Enterobacteriaceae (2x Providencia sp., 2x Salmonella sp., 7 Citrobacter sp., 9 Proteus sp., 15 Klebsiella sp., 17 Enterobacter sp.) were correctly not identified as E. coli by this test. Bactident E. coli is a suitable test for rapid identification of E. coli in veterinary bacteriology.  相似文献   

Biochemical behavior and sensitivity to antibiotics of Klebsiella pathogens, isolated from horse test material]     

A Weber  U Neumeier  T Schilesser 《Berliner und Münchener tier?rztliche Wochenschrift》1975,88(7):121-123

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Comparison of sire evaluation methods for carcass gain using field data     

M. Schneeberger  C. Gaillard  N. Künzi  F. Weber 《Livestock Production Science》1977,4(1):11-17

Four methods of predicting breeding values for carcass gain are compared: “regressed” least squares (RLSQ), mixed model (MM), mixed model with relationships among sires considered (MMR) and contemporary-comparison (CC). Very small changes in the ranking of the sires were observed from one method to another (rank correlations > 0.96). Consideration of the relationships among the sires or deleting the breed-group factor from the model did not lead to substantially different breeding values. The good correspondence of the predictors is mainly due to the well balanced distribution of the progeny over herds.  相似文献   

In vitro viral expression as a criterion for development of control procedures for enzootic bovine leukosis     

A F Weber  J C Meiske  E C Hooker  D L Haggard  A M Domagala  D K Sorensen  L C Buoen 《American journal of veterinary research》1987,48(6):899-903

In a university beef herd of 304 cattle in which six died of lymphosarcoma between 1980 and 1984, 77% of the Angus and 26% of the Charolais cattle were determined to be infected with bovine leukemia virus (BLV). Changes in iatrogenic procedures were initiated as early control measures. In vitro viral expression (VE) was used as a criterion to identify cattle for subsequent segregation or culling. This involved determinations of percentages of BLV-associated lymphocyte profiles among thin-sectioned Ficoll-Paque-isolated blood lymphocytes that were processed into plastic after culture for 48 h. Cattle retained until completion of nutritional studies or as breeding stock were separated into two groups. The BLV-seronegative cattle, BLV-seropositive cattle with 0% VE, and BLV-seropositive cattle with 1% to 4% VE were placed in group 1. Seropositive cattle with greater than or equal to 5% VE were placed in group 2. In 1985, evaluation of in vitro VE in 108 mature BLV-seropositive cattle retained for breeding revealed 36 (33%) had no observable VE. In 1986, 58 of 108 cattle were available to be reexamined, and 21 (36%) had 0% VE in both years. The VE expression values for individual cattle were generally comparable over the 2-year period. Of 48 initial seronegative breeding stock housed in group 1 with BLV-seropositive cattle with low or no VE, 21 (44%) seroconverted during 1985 to 1986. A positive correlation of 0.585 was found between VE and age-related absolute lymphocyte number.  相似文献   

Biochemical and serological identification of Yersinia from birds     

A Weber  G Glünder  K H Hinz 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1987,34(2):148-154

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Isolierung von Brucella canis aus Citratblut über das embryonierte Hühnerei*     

A. Weber  Th. Schliesser 《Zoonoses and public health》1977,24(7):576-581

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Studies on the Maturation and Spawning of Milkfish Chanos chanos Forsskal in a Photoperiod-Controlled Room     

Cheng-Sheng  Lee Clyde S.  Tamaru G. M. Weber 《Journal of the World Aquaculture Society》1987,18(4):253-259

Progress in propagation of milkfish has been impeded by the shortage of mature broodstock. This study was a first step toward revealing the environmental cues for the maturation of milkfish. Experiments were carried out during 1978-1985 in an indoor tank (5.2 × 4.8 × 13 m) under photoperiod control and in an outdoor tank (63 × 5.4 × 13 m). In two controlled, long-photoperiod regime experiments, 86 and 83% of the fish matured, the highest percentages yet reported. In these experiments, milkfish matured one month earlier than the normal spawning season, two months after the fish were exposed to the long daylight regime. Only 21% of the fish under a natural photoperiod regime in the outdoor tank matured. Results of the current study suggest that a change and increase in photoperiod are necessary for the onset of maturation.  相似文献