光照对鸡产蛋性能的影响及其机理研究——Ⅲ.光照对鸡大脑皮质电位的影响     

胡仲明  柳巨雄  张玉生 《中国兽医学报》1987,(4)

选用22只“星杂579”成龄母鸡,以电生理方法观察了光照(30lx)及在麻醉状态下光照(30lx)和黑暗对其大脑皮质“Wulst”区电位的影响。结果,黑暗时的电位可因光照而抑制;注射氯胺酮麻醉后,电位明显变小,再给黑暗和光照,电位均没有变化。表明大脑皮质参与了光照对鸡的生理调节过程。  相似文献   

‘Plantibodies’: a flexible approach to design resistance against pathogens     

A. Schots  J. De Boer  A. Schouten  J. Roosien  J. F. Zil Verentant  H. Pomp  L. Bouwman-Smits  H. Overmars  F. J. Gommers  B. Visser  W. J. Stiekema  J. Bakker 《European journal of plant pathology / European Foundation for Plant Pathology》1992,98(2):183-191

Engineering resistance against various diseases and pests is hampered by the lack of suitable genes. To overcome this problem we started a research program aimed at obtaining resistance by transfecting plants with genes encoding monoclonal antibodies against pathogen specific proteins. The idea is that monoclonal antibodies will inhibit the biological activity of molecules that are essential for the pathogenesis. Potato cyst nematodes are chosen as a model and it is thought that monoclonal antibodies are able to block the function of the saliva proteins of this parasite. These proteins are, among others, responsible for the induction of multinucleate transfer cells upon which the nematode feeds. It is well documented that the ability of antibodies to bind molecules is sufficient to inactivate the function of an antigen and in view of the potential of animals to synthesize antibodies to almost any molecular structure, this strategy should be feasible for a wide range of diseases and pests.Antibodies have several desirable features with regard to protein engineering. The antibody (IgG) is a Y-shaped molecule, in which the domains forming the tips of the arms bind to antigen and those forming the stem are responsible for triggering effector functions (Fc fragments) that eliminate the antigen from the animal. Domains carrying the antigen-binding loops (Fv and Fab fragments) can be used separately from the Fc fragments without loss of affinity. The antigen-binding domains can also be endowed with new properties by fusing them to toxins or enzymes. Antibody engineering is also facilitated by the Polymerase Chain Reaction (PCR). A systematic comparison of the nucleotide sequence of more than 100 antibodies revealed that not only the 3′-ends, but also the 5′-ends of the antibody genes are relatively conserved. We were able to design a small set of primers with restriction sites for forced cloning, which allowed the amplification of genes encoding antibodies specific for the saliva proteins ofGlobodera rostochiensis. Complete heavy and light chain genes as well as single chain Fv fragments (scFv), in which the variable parts of the light (V_L) and heavy chain (V_H) are linked by a peptide, will be transferred to potato plants. A major challenge will be to establish a correct expression of the antibody genes with regard to three dimensional folding, assembly and intracellular location.  相似文献   

甜杂二号甜椒的选育   总被引：2，自引：0，他引：2  

胡洽  俞世敏 《华北农学报》1990,5(4):72-76

甜杂二号甜椒为新育成的一代杂种,其植株生长势旺、早熟、丰产、座果率高,连续结实性好,抗TMV病毒能力强.果实绿色灯笼形,味甜质脆,适宜保护地栽培,比对照茄门,同丰37、同丰16、早熟3号等增产31%以上,增值26%以上.1987年通过北京市品种审定.现已成为北京保护地甜椒生产主栽品种之一.全国20个省市均有试种,反映良好.  相似文献   

套袋对黄金梨果实品质的影响   总被引：7，自引：0，他引：7  

赵学常  胡学峰  徐桂云 《落叶果树》2005,37(3):11-12

对黄金梨果实进行了套袋试验表明，供试果袋均能改善黄金梨的果实品质，其中以套小林袋和山东省果树研究所生产的外浅褐内红双层纸袋效果较好，果实外观显著改善，果实硬度为对照的1．26倍；果实可溶性固形物含量略有下降，与对照相比下降0．88～1．06个百分点。  相似文献   

两种光周期下矮牵牛‘幻想粉红’生长发育特性的研究   总被引：3，自引：2，他引：3  

胡惠蓉  刘亚红  胡晓龙  包满珠 《园艺学报》2005,32(4):719-721

 将矮牵牛‘幻想粉红’分别培养在长日照和短日照条件下, 观测其始花期及株高、株幅、分枝数和开花数的变化; 将短日照下培养的‘幻想粉红’分别于2、4、6、8、10、12、14、16片真叶时移入长日照下进行培养, 观测从长日照处理至第1朵花开放的时间。结果分析表明: 长日照下, ‘幻想粉红’株型松散, 短日照下生长紧凑; ‘幻想粉红’为量性长日照植物, 短日照下要达到较大的营养生长量才能开花; ‘幻想粉红’的幼龄期约在8片真叶期结束。初步建议在8叶期之前采用短日照处理‘幻想粉红’,以得到紧凑的株型, 而在8叶期以后给予长日照, 以促进开花。  相似文献   

杨桃果实生长发育过程中营养品质的变化   总被引：8，自引：0，他引：8  

胡志群  周碧燕  陈杰忠  林小冰  蓝永辉 《园艺学报》2005,32(5):782-782

杨桃（Averrhoa arambola L．）为华南地区重要的热带水果。本试材为华南农业大学园艺分场种植的3年生盆栽马来西亚甜杨桃，砧木为酸杨桃。在杨桃的开花期，选择生长势、开花数量和时间较一致45株为试材，再选择受精时间一致的果实做标记。采样时问从开花后（2003年7月15日）至杨桃充分成熟（2003年10月25）。测定杨桃果实中的糖（蒽酮比色法）、有机酸（HPLC法）、维生素C（2，6-二氯靛酚钠滴定法）和单宁（高锰酸钾滴定法）的含量。  相似文献   

《动物防疫法》与当蓟工作不相适应的问题及建议     

胡书凌 《中国动物检疫》2005,22(3):7-7,14

《动物防疫法》自1998年1月1日实施至今已有7年了，其在加强动物防疫工作的管理及预防、控制和扑灭动物疫病，促进养殖业发展，保护人体健康方面发挥了积极的作用。但在实际工作中，我们常常感到《动物防疫法》中的一些条款规定不明确、不具体，造成可操作性差，难以落实；同时一些罚则设置不当，处罚力度小，与相关法律衔接不上，起不到应有的作用。  相似文献   

Cell proliferation and nestin expression in cortex and SEZ of MCAO rats     

CHEN Hu  CAI Ding-fang  LIANG Hui  CHENG Jie-shi 《园艺学报》2003,19(7):917-919

AIM:To explore the effect of brain ischemia injury on cell proliferation and nestin expression in cortex and subependymal zone (SEZ).METHODS:Using a local brain ischemia model(MCAO), BrdU positive cells of cortex and subependymal zone (SEZ), also nestin positive cells, were observed by immunohistochemistry.RESULTS:BrdU and nestin positive cells in SEZ of MCAO rats were obviously increased. In cortex, only nestin positive cells were observed.CONCLUSION:Neural stem cells in SEZ and cortex were activated after brain ischemia, it may be related with neural recovery after brain ischemia injury.  相似文献   

冷藏和气调处理对青花菜 Glucoraphanin 含量的影响     

汪俏梅  胡家恕 《园艺学报》2003,30(3):286-286

Glucoraphanin (4-甲基亚磺酰丁基芥子油苷 )在青花菜中含量丰富 ,其降解产物Sulforaphane具很强的抗癌活性。本试验以‘Marathon’青花菜为试材 ,将新鲜花球切成小花球后用 10 0mg/L的NaClO表面消毒 1min ,清水洗净。分别取 6 5 0g花球置于 0℃、 5℃和 10℃冷藏 ,并在 5℃下进行 5种气调处理 :1)Air,2 )Air+10 %CO2 ,3)Air+2 0 %CO2 ,4) 1%O2 ,5 ) 1%O2 +10 %CO2 。每一处理分别在处理当天、处理后 3、 6、 9、 12d取样 5 0g ,真空冷冻干燥 ,并研磨成粉末状贮藏于 4℃待用。将 0 .2g冷冻干燥样品在沸水中煮 10min ,35 0 0g离心 5m…  相似文献   

优质高产菜豆新品种‘无筋2号''   总被引：2，自引：0，他引：2  

毛伟海  包崇来  孙丽霞  胡天华  胡齐赞 《园艺学报》2003,30(4):501-501

‘无筋2号’为优质高产菜豆新品种，生长势旺盛，耐肥抗倒，播种至采鲜荚生育期45d左右，结荚性好，豆荚饱满，商品性好，适宜速冻加工出口，鲜荚产量17—18t/hm^2，高产地块可达20t/hm^2以上。  相似文献